TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBE1h10.xg.2.1
(463 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK320389.1|CK320389 L3P05d10 Populus stem seasonal libra... 141 2e-034
gb|DT517940.1|DT517940 WS02435.B21_L21 PTxD-ICC-N-A-14 Popu... 139 6e-034
gb|DT516146.1|DT516146 WS02430.B21_I16 PTxD-ICC-N-A-14 Popu... 139 6e-034
gb|DT514871.1|DT514871 WS02426.B21_A05 PTxD-ICC-N-A-14 Popu... 139 6e-034
gb|DT509002.1|DT509002 WS02422.BR_I07 PTxD-ICC-N-A-14 Popul... 139 6e-034
gb|DT513609.1|DT513609 WS02422.B21_I07 PTxD-ICC-N-A-14 Popu... 139 6e-034
gb|DN493003.1|DN493003 X051B06.3pR Populus wood cDNA librar... 121 4e-027
>gb|CK320389.1|CK320389 L3P05d10 Populus stem seasonal library Populus deltoides cDNA, mRNA
sequence
Length = 832
Score = 141 bits (303), Expect(2) = 2e-034
Identities = 58/101 (57%), Positives = 70/101 (69%)
Frame = +2 / +2
Query: 68 KPKVLMCTDPTKXXLSPDLYKPTFXXFVDADISXXXITLRSLIDRSXVESFGAGGKTCIL 247
K KVL+C+D L LYKP+F FVD D++ ++LRSLID S VESFGAGG+T I
Sbjct: 347 KHKVLLCSDARSSSLGEGLYKPSFAGFVDVDLTDKKLSLRSLIDHSVVESFGAGGRTAIT 526
Query: 248 SRVYPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMN 370
SRVYP+IAV + AHLYVFNNG +T L AW M P+MN
Sbjct: 527 SRVYPTIAVFEKAHLYVFNNGSETITVENLNAWSMNLPVMN 649
Score = 23.5 bits (45), Expect(2) = 2e-034
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +2 / +2
Query: 5 ASEDLQERXXXFFRVFR 55
ASE+L+E FF+VF+
Sbjct: 287 ASENLEEFTPVFFKVFK 337
>gb|DT517940.1|DT517940 WS02435.B21_L21 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02435_L21 3', mRNA sequence
Length = 904
Score = 139 bits (298), Expect(2) = 6e-034
Identities = 60/101 (59%), Positives = 68/101 (67%)
Frame = +2 / -2
Query: 68 KPKVLMCTDPTKXXLSPDLYKPTFXXFVDADISXXXITLRSLIDRSXVESFGAGGKTCIL 247
K KVL+C+D L LYKP F FVD D++ +TLRSLID S VESFGAGG+T I
Sbjct: 438 KHKVLLCSDARSSSLGVGLYKPPFAGFVDVDLTDKKLTLRSLIDHSVVESFGAGGRTVIT 259
Query: 248 SRVYPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMN 370
SRVYP IAV AHL+VFNNG VT L AW MK P+MN
Sbjct: 258 SRVYPIIAVFDKAHLFVFNNGSETVTVETLNAWSMKVPVMN 136
Score = 24.0 bits (46), Expect(2) = 6e-034
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / -2
Query: 5 ASEDLQERXXXFFRVFR 55
ASE L+E FFRVF+
Sbjct: 498 ASEKLEEFTPVFFRVFK 448
>gb|DT516146.1|DT516146 WS02430.B21_I16 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02430_I16 3', mRNA sequence
Length = 893
Score = 139 bits (298), Expect(2) = 6e-034
Identities = 60/101 (59%), Positives = 68/101 (67%)
Frame = +2 / -2
Query: 68 KPKVLMCTDPTKXXLSPDLYKPTFXXFVDADISXXXITLRSLIDRSXVESFGAGGKTCIL 247
K KVL+C+D L LYKP F FVD D++ +TLRSLID S VESFGAGG+T I
Sbjct: 436 KHKVLLCSDARSSSLGVGLYKPPFAGFVDVDLTDKKLTLRSLIDHSVVESFGAGGRTVIT 257
Query: 248 SRVYPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMN 370
SRVYP IAV AHL+VFNNG VT L AW MK P+MN
Sbjct: 256 SRVYPIIAVFDKAHLFVFNNGSETVTVETLNAWSMKVPVMN 134
Score = 24.0 bits (46), Expect(2) = 6e-034
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / -2
Query: 5 ASEDLQERXXXFFRVFR 55
ASE L+E FFRVF+
Sbjct: 496 ASEKLEEFTPVFFRVFK 446
>gb|DT514871.1|DT514871 WS02426.B21_A05 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02426_A05 3', mRNA sequence
Length = 857
Score = 139 bits (298), Expect(2) = 6e-034
Identities = 60/101 (59%), Positives = 68/101 (67%)
Frame = +2 / -1
Query: 68 KPKVLMCTDPTKXXLSPDLYKPTFXXFVDADISXXXITLRSLIDRSXVESFGAGGKTCIL 247
K KVL+C+D L LYKP F FVD D++ +TLRSLID S VESFGAGG+T I
Sbjct: 431 KHKVLLCSDARSSSLGVGLYKPPFAGFVDVDLTDKKLTLRSLIDHSVVESFGAGGRTVIT 252
Query: 248 SRVYPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMN 370
SRVYP IAV AHL+VFNNG VT L AW MK P+MN
Sbjct: 251 SRVYPIIAVFDKAHLFVFNNGSETVTVETLNAWSMKVPVMN 129
Score = 24.0 bits (46), Expect(2) = 6e-034
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / -1
Query: 5 ASEDLQERXXXFFRVFR 55
ASE L+E FFRVF+
Sbjct: 491 ASEKLEEFTPVFFRVFK 441
>gb|DT509002.1|DT509002 WS02422.BR_I07 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02422_I07 5', mRNA sequence
Length = 634
Score = 139 bits (298), Expect(2) = 6e-034
Identities = 60/101 (59%), Positives = 68/101 (67%)
Frame = +2 / +2
Query: 68 KPKVLMCTDPTKXXLSPDLYKPTFXXFVDADISXXXITLRSLIDRSXVESFGAGGKTCIL 247
K KVL+C+D L LYKP F FVD D++ +TLRSLID S VESFGAGG+T I
Sbjct: 209 KHKVLLCSDARSSSLGVGLYKPPFAGFVDVDLTDKKLTLRSLIDHSVVESFGAGGRTVIT 388
Query: 248 SRVYPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMN 370
SRVYP IAV AHL+VFNNG VT L AW MK P+MN
Sbjct: 389 SRVYPIIAVFDKAHLFVFNNGSETVTVETLNAWSMKVPVMN 511
Score = 24.0 bits (46), Expect(2) = 6e-034
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / +2
Query: 5 ASEDLQERXXXFFRVFR 55
ASE L+E FFRVF+
Sbjct: 149 ASEKLEEFTPVFFRVFK 199
>gb|DT513609.1|DT513609 WS02422.B21_I07 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02422_I07 3', mRNA sequence
Length = 634
Score = 139 bits (298), Expect(2) = 6e-034
Identities = 60/101 (59%), Positives = 68/101 (67%)
Frame = +2 / -2
Query: 68 KPKVLMCTDPTKXXLSPDLYKPTFXXFVDADISXXXITLRSLIDRSXVESFGAGGKTCIL 247
K KVL+C+D L LYKP F FVD D++ +TLRSLID S VESFGAGG+T I
Sbjct: 426 KHKVLLCSDARSSSLGVGLYKPPFAGFVDVDLTDKKLTLRSLIDHSVVESFGAGGRTVIT 247
Query: 248 SRVYPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMN 370
SRVYP IAV AHL+VFNNG VT L AW MK P+MN
Sbjct: 246 SRVYPIIAVFDKAHLFVFNNGSETVTVETLNAWSMKVPVMN 124
Score = 24.0 bits (46), Expect(2) = 6e-034
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / -2
Query: 5 ASEDLQERXXXFFRVFR 55
ASE L+E FFRVF+
Sbjct: 486 ASEKLEEFTPVFFRVFK 436
>gb|DN493003.1|DN493003 X051B06.3pR Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA clone X051B06 3', mRNA sequence
Length = 780
Score = 121 bits (258), Expect = 4e-027
Identities = 47/98 (47%), Positives = 65/98 (66%)
Frame = +2 / -2
Query: 77 VLMCTDPTKXXLSPDLYKPTFXXFVDADISXXXITLRSLIDRSXVESFGAGGKTCILSRV 256
VLMC+D ++ L ++ K T+ F+D D I+LRSLID S +ESFG G+ CI +RV
Sbjct: 638 VLMCSDQSRSALRDEVDKTTYGAFIDIDPQRENISLRSLIDHSIIESFGGEGRACITNRV 459
Query: 257 YPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMN 370
YP +A+ ++AHL++FNNG VT S L AW M K +N
Sbjct: 458 YPKLAIHEEAHLFIFNNGTLSVTISSLNAWSMNKAQIN 345
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 139,826,506
Number of Sequences: 369679
Number of extensions: 1121446
Number of successful extensions: 28854
Number of sequences better than 1.0e-020: 7
length of database: 67,802,888
effective HSP length: 50
effective length of database: 49,318,938
effective search space used: 5079850614
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)