TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBD3a09.xg.3.1
(1105 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DN484173.1|DN484173 C028P10.3pR Populus strain T89 leave... 147 3e-061
gb|CK089218.1|CK089218 B005P52.3pR Hybrid aspen plasmid lib... 131 1e-056
gb|DN493529.1|DN493529 C028P10.5pR Populus strain T89 leave... 119 3e-026
gb|BU881837.1|BU881837 UM68TB10 Populus flower cDNA library... 110 2e-023
>gb|DN484173.1|DN484173 C028P10.3pR Populus strain T89 leaves Populus tremula x Populus
tremuloides cDNA clone C028P10 3', mRNA sequence
Length = 642
Score = 147 bits (315), Expect(2) = 3e-061
Identities = 62/100 (62%), Positives = 79/100 (79%)
Frame = +1 / -2
Query: 352 SVVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAI 531
++VE FL+E G+DE +K +I+AIIKGMGFK E++ + E + EF +VQDADRLDAIGA+
Sbjct: 560 TIVENFLEEEGVDEVKKMKILAIIKGMGFKEELAGHANGEFSPEFRVVQDADRLDAIGAV 381
Query: 532 GIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQT 651
GIARCFT+GGS+N LHDP I PR LSK +YM KEE+ T
Sbjct: 380 GIARCFTFGGSRNRLLHDPAIHPRSDLSKAQYMKKEEQTT 261
Score = 109 bits (232), Expect(2) = 3e-061
Identities = 40/54 (74%), Positives = 51/54 (94%)
Frame = +1 / -2
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTEAGKRRAEKRHRFMEEFVAEFYEEWSGRA* 795
K+E+QT++NHFHEKL KLKD+MKT+AG+RRAE+RH+FME+F+ EFYEEW GRA*
Sbjct: 281 KKEEQTTVNHFHEKLLKLKDLMKTKAGQRRAERRHKFMEDFLKEFYEEWDGRA* 120
Score = 107 bits (229), Expect(2) = 1e-034
Identities = 56/102 (54%), Positives = 64/102 (62%)
Frame = -3 / +1
Query: 650 VCFSSLDMYFSFDKLSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVG 471
V SS +Y +FDK RG I GS + LFLLPP VKH AIP APIAS RSASCT NS
Sbjct: 262 VVCSSFFIYCAFDKSERG*IAGS*SNLFLLPPKVKHRAIPTAPIASSRSASCTTLNSGEN 441
Query: 470 STIDLFETSFLNPIPLIIATISSFWPSSNPTS*RNISTTLIS 345
S +S L PIPL+IA I F+ SS P+S R ST + S
Sbjct: 442 SPFACPASSSLKPIPLMIARIFIFFTSSTPSSSRKFSTIVSS 567
Score = 59.3 bits (123), Expect(2) = 1e-034
Identities = 22/49 (44%), Positives = 30/49 (61%)
Frame = -2 / +3
Query: 795 SSPATPLFVEFSHKFLHEPMPFLSSPFPGLCLHHVLQFKKLLMKMID*C 649
SS + P F+E K LHE + LS P PGLC H +LQ +K M+++ C
Sbjct: 120 SSSSIPFFIELFQKILHELVSSLSPPLPGLCFHQILQLEKFFMEVVHSC 266
>gb|CK089218.1|CK089218 B005P52.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone B005P52 3', mRNA sequence
Length = 569
Score = 131 bits (281), Expect(2) = 1e-056
Identities = 56/87 (64%), Positives = 68/87 (78%)
Frame = +1 / -3
Query: 391 EGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIGIARCFTYGGSKN 570
E +K +I+AIIKGMGFK E++ + E + EF +VQDADRLD IGA+GIARCFT+GGS+N
Sbjct: 567 EVKKMKILAIIKGMGFKEELAGHANGEFSPEFRVVQDADRLDPIGAVGIARCFTFGGSRN 388
Query: 571 SALHDPRILPRDSLSKEKYMSKEEKQT 651
LHDP I PR LSKEKYM KEE+ T
Sbjct: 387 RMLHDPAIHPRSDLSKEKYMKKEEQTT 307
Score = 109 bits (232), Expect(2) = 1e-056
Identities = 40/54 (74%), Positives = 51/54 (94%)
Frame = +1 / -3
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTEAGKRRAEKRHRFMEEFVAEFYEEWSGRA* 795
K+E+QT++NHFHEKL KLKD+MKT+AG+RRAE+RH+FME+F+ EFYEEW GRA*
Sbjct: 327 KKEEQTTVNHFHEKLLKLKDLMKTKAGQRRAERRHKFMEDFLKEFYEEWDGRA* 166
Score = 101 bits (215), Expect(2) = 3e-031
Identities = 51/87 (58%), Positives = 56/87 (64%)
Frame = -3 / +2
Query: 650 VCFSSLDMYFSFDKLSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVG 471
V SS +YFSFDK RG I GS + LFLLPP VKHLAIP API S RSASCT NS
Sbjct: 308 VVCSSFFIYFSFDKSERG*IAGS*SILFLLPPKVKHLAIPTAPIGSSRSASCTTLNSGEN 487
Query: 470 STIDLFETSFLNPIPLIIATISSFWPS 390
S +S L PIPL+IA I F+ S
Sbjct: 488 SPFACPASSSLKPIPLMIARIFIFFTS 568
Score = 54.7 bits (113), Expect(2) = 3e-031
Identities = 20/49 (40%), Positives = 28/49 (57%)
Frame = -2 / +1
Query: 795 SSPATPLFVEFSHKFLHEPMPFLSSPFPGLCLHHVLQFKKLLMKMID*C 649
SS + P F+E K LHE + L P P LC H +LQ +K M+++ C
Sbjct: 166 SSSSIPFFIELFQKILHELVSSLGPPLPSLCFHQILQLEKFFMEVVHSC 312
>gb|DN493529.1|DN493529 C028P10.5pR Populus strain T89 leaves Populus tremula x Populus
tremuloides cDNA clone C028P10 5', mRNA sequence
Length = 599
Score = 119 bits (255), Expect = 3e-026
Identities = 50/78 (64%), Positives = 64/78 (82%)
Frame = +1 / +1
Query: 355 VVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIGAIG 534
+VE FL+E G+DE +K +I+AIIKGMGFK E++ + E + EF +VQDADRLDAIGA+G
Sbjct: 364 IVENFLEEEGVDEVKKMKILAIIKGMGFKEELAGHANGEFSPEFRVVQDADRLDAIGAVG 543
Query: 535 IARCFTYGGSKNSALHDP 588
IARCFT+GGS+N LHDP
Sbjct: 544 IARCFTFGGSRNRWLHDP 597
>gb|BU881837.1|BU881837 UM68TB10 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 337
Score = 110 bits (235), Expect = 2e-023
Identities = 41/54 (75%), Positives = 51/54 (94%)
Frame = +1 / +2
Query: 634 KEEKQTSINHFHEKLFKLKDMMKTEAGKRRAEKRHRFMEEFVAEFYEEWSGRA* 795
K+E+QT++NHFHEKL KLKD+MKT+AG+RRAE+RH+FMEEF+ EFYEEW GRA*
Sbjct: 14 KKEEQTTVNHFHEKLLKLKDLMKTKAGQRRAERRHKFMEEFLKEFYEEWDGRA* 175
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 513,700,380
Number of Sequences: 369679
Number of extensions: 7125068
Number of successful extensions: 49002
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 53
effective length of database: 48,209,901
effective search space used: 15137908914
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)