TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF13b04.yg.2.1
(370 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6... 138 1e-032
gb|AJ776567.1|AJ776567 AJ776567 Populus euphratica root 3-6... 133 3e-031
gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, compl... 81 4e-029
gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZA... 120 5e-027
gb|AI166257.1|AI166257 xylem.est.107 Poplar xylem Lambda ZA... 116 4e-026
gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Po... 107 3e-023
>gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6 months, adult Populus
euphratica cDNA clone P0001100006E01F1, mRNA sequence
Length = 295
Score = 138 bits (297), Expect = 1e-032
Identities = 55/69 (79%), Positives = 62/69 (89%)
Frame = +3 / -1
Query: 144 IGVFTAVPMILGFILESGVLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKY 323
IG+FTAVPM+LGFILE G L A V FITMQ Q+CSVFFTFSLGT++HYFGR ILHGGA+Y
Sbjct: 295 IGIFTAVPMVLGFILELGFLRAVVSFITMQLQLCSVFFTFSLGTKSHYFGRTILHGGARY 116
Query: 324 RATGRGFVV 350
+ATGRGFVV
Sbjct: 115 QATGRGFVV 89
>gb|AJ776567.1|AJ776567 AJ776567 Populus euphratica root 3-6 months Populus euphratica cDNA
clone P0000400015B12F1, mRNA sequence
Length = 468
Score = 133 bits (286), Expect = 3e-031
Identities = 55/111 (49%), Positives = 71/111 (63%)
Frame = +3 / +3
Query: 18 YIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESG 197
Y+FLYG++YL LSG+ + + + I +Q QIG A+PM++ LE G
Sbjct: 72 YVFLYGRLYLVLSGLEKGLSTQRAIRDNKALQVALASQSFVQIGFLMALPMMMEIGLEKG 251
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
A FI MQ Q+ VFFTFSLGT+THY+GR +LHGG+ YRATGRGFVV
Sbjct: 252 FHNALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGSAYRATGRGFVV 404
>gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, complete sequence
Length = 96953
Score = 81.3 bits (171), Expect(3) = 4e-029
Identities = 31/41 (75%), Positives = 36/41 (87%)
Frame = +3 / +2
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAK 320
+L A V FITMQ Q+CSVFFTFSLGT++HYFGR ILHGGA+
Sbjct: 27899 MLQAVVNFITMQLQLCSVFFTFSLGTKSHYFGRTILHGGAR 28021
Score = 73.0 bits (153), Expect(3) = 4e-029
Identities = 32/55 (58%), Positives = 38/55 (69%)
Frame = +3 / +1
Query: 39 MYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESGVL 203
++ ALSGVGE ++ RA I TQFLFQIG+FTAVPM+LGFILE G L
Sbjct: 27157 LF*ALSGVGEEVEIRALITKNNALSAALNTQFLFQIGIFTAVPMVLGFILELGFL 27321
Score = 24.9 bits (48), Expect(3) = 4e-029
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +3 / +3
Query: 321 YRATGRGFVV 350
Y+ATGRGFVV
Sbjct: 28110 YQATGRGFVV 28139
>gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZAPII library Populus trichocarpa
cDNA 5', mRNA sequence
Length = 645
Score = 120 bits (256), Expect = 5e-027
Identities = 51/102 (50%), Positives = 65/102 (63%)
Frame = +3 / +1
Query: 45 LALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESGVLTAFVQFI 224
L LSG+ E + + I +Q QIG A+PM++ LE G TA +F+
Sbjct: 1 LVLSGLEEGLSTQKAIRDNKPLQVALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFL 180
Query: 225 TMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
MQ Q+ VFFTFSLGT+THY+GR +LHGGAKYR+TGRGFVV
Sbjct: 181 LMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVV 306
>gb|AI166257.1|AI166257 xylem.est.107 Poplar xylem Lambda ZAPII library Populus trichocarpa
cDNA 5', mRNA sequence
Length = 583
Score = 116 bits (249), Expect = 4e-026
Identities = 48/101 (47%), Positives = 64/101 (63%)
Frame = +3 / +2
Query: 18 YIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESG 197
Y+FLYG++YL LSG+ E + + I +Q QIG A+PM++ LE G
Sbjct: 272 YVFLYGRLYLVLSGLEEGLSTQKAIRDNKPLQVALASQSFVQIGFLMALPMLMEIGLERG 451
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAK 320
TA +FI MQ Q+ VFFTFSLGT+THY+GR +LHGGA+
Sbjct: 452 FRTALSEFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAE 574
>gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 467
Score = 107 bits (229), Expect = 3e-023
Identities = 44/75 (58%), Positives = 53/75 (70%)
Frame = +3 / +2
Query: 126 TQFLFQIGVFTAVPMILGFILESGVLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAIL 305
+Q QIG A+PM++ LE G A FI MQ Q+ VFFTFSLGT+THY+GR +L
Sbjct: 29 SQSFVQIGFLMALPMMMEIGLEKGFRNALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLL 208
Query: 306 HGGAKYRATGRGFVV 350
HGG+ YRATGRGFVV
Sbjct: 209 HGGSAYRATGRGFVV 253
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 206,907,766
Number of Sequences: 369679
Number of extensions: 3052324
Number of successful extensions: 156861
Number of sequences better than 1.0e-020: 6
length of database: 67,802,888
effective HSP length: 49
effective length of database: 49,688,617
effective search space used: 3627269041
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)