TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE8g03.yg.2.1
(360 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV283626.1|CV283626 WS0187.B21_N13 PTxD-IL-N-A-9 Populus... 90 9e-028
gb|BU873883.1|BU873883 Q061A02 Populus flower cDNA library ... 90 9e-028
gb|CK095962.1|CK095962 UA38BPF06.3pR Populus dormant cambiu... 90 2e-027
gb|BI122326.1|BI122326 I005P20P Populus leaf cDNA library P... 90 2e-025
gb|BU819039.1|BU819039 UA38BPF06 Populus tremula cambium cD... 78 6e-022
>gb|CV283626.1|CV283626 WS0187.B21_N13 PTxD-IL-N-A-9 Populus trichocarpa x Populus
deltoides cDNA clone WS0187_N13 3', mRNA sequence
Length = 607
Score = 90.0 bits (190), Expect(3) = 9e-028
Identities = 35/53 (66%), Positives = 44/53 (83%)
Frame = -3 / -1
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGVTEKGSEGLLRS 56
RYLFVRW L V NM++I GE+GDTD+EEM+SG HKT+I++ V KGSE LLR+
Sbjct: 484 RYLFVRWRLNVANMFVILGENGDTDYEEMISGAHKTIILKDVVTKGSEDLLRT 326
Score = 43.7 bits (89), Expect(3) = 9e-028
Identities = 17/21 (80%), Positives = 20/21 (95%)
Frame = -3 / -1
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTRLQ++P LASR+QALR
Sbjct: 544 CRNSTRLQIIPHLASRAQALR 482
Score = 24.9 bits (48), Expect(3) = 9e-028
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -3 / -1
Query: 40 KDDVVPSETPLAA 2
+DD+VP E+PL A
Sbjct: 316 RDDIVPKESPLIA 278
>gb|BU873883.1|BU873883 Q061A02 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 616
Score = 90.0 bits (190), Expect(3) = 9e-028
Identities = 35/53 (66%), Positives = 44/53 (83%)
Frame = -3 / +1
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGVTEKGSEGLLRS 56
RYLFVRW L V NM++I GE+GDTD+EEM+SG HKT+I++ V KGSE LLR+
Sbjct: 199 RYLFVRWRLNVANMFVILGENGDTDYEEMISGAHKTIILKDVVTKGSEDLLRA 357
Score = 43.7 bits (89), Expect(3) = 9e-028
Identities = 17/21 (80%), Positives = 20/21 (95%)
Frame = -3 / +1
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTRLQ++P LASR+QALR
Sbjct: 139 CRNSTRLQIIPHLASRAQALR 201
Score = 24.9 bits (48), Expect(3) = 9e-028
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -3 / +1
Query: 40 KDDVVPSETPLAA 2
+DD+VP E+PL A
Sbjct: 367 RDDIVPKESPLIA 405
>gb|CK095962.1|CK095962 UA38BPF06.3pR Populus dormant cambium cDNA library Populus tremula
cDNA clone UA38BPF06 3', mRNA sequence
Length = 764
Score = 90.0 bits (190), Expect(3) = 2e-027
Identities = 35/53 (66%), Positives = 44/53 (83%)
Frame = -3 / -1
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGVTEKGSEGLLRS 56
RYLFVRW L V NM++I GE+GDTD+EEM+SG HKT+I++ V KGSE LLR+
Sbjct: 473 RYLFVRWRLNVANMFVILGENGDTDYEEMISGAHKTIILKDVVTKGSEDLLRA 315
Score = 43.7 bits (89), Expect(3) = 2e-027
Identities = 17/21 (80%), Positives = 20/21 (95%)
Frame = -3 / -1
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTRLQ++P LASR+QALR
Sbjct: 533 CRNSTRLQIIPHLASRAQALR 471
Score = 24.9 bits (48), Expect(3) = 2e-027
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -3 / -1
Query: 40 KDDVVPSETPLAA 2
+DD+VP E+PL A
Sbjct: 305 RDDIVPKESPLIA 267
>gb|BI122326.1|BI122326 I005P20P Populus leaf cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 486
Score = 90.0 bits (190), Expect(2) = 2e-025
Identities = 35/53 (66%), Positives = 44/53 (83%)
Frame = -3 / +3
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGVTEKGSEGLLRS 56
RYLFVRW L V NM++I GE+GDTD+EEM+SG HKT+I++ V KGSE LLR+
Sbjct: 300 RYLFVRWRLNVANMFVILGENGDTDYEEMISGAHKTIILKDVVTKGSEDLLRA 458
Score = 43.7 bits (89), Expect(2) = 2e-025
Identities = 17/21 (80%), Positives = 20/21 (95%)
Frame = -3 / +3
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTRLQ++P LASR+QALR
Sbjct: 240 CRNSTRLQIIPHLASRAQALR 302
>gb|BU819039.1|BU819039 UA38BPF06 Populus tremula cambium cDNA library Populus tremula cDNA
5 prime, mRNA sequence
Length = 511
Score = 78.0 bits (164), Expect(2) = 6e-022
Identities = 29/42 (69%), Positives = 36/42 (85%)
Frame = -3 / +1
Query: 214 RYLFVRWGLYVGNMYLITGEHGDTDHEEMLSGLHKTVIVRGV 89
RYLFVRW L V NM++I GE+GDTDHEEM+SG HKT+I++ V
Sbjct: 382 RYLFVRWRLNVANMFVILGENGDTDHEEMISGAHKTIILKDV 507
Score = 43.7 bits (89), Expect(2) = 6e-022
Identities = 17/21 (80%), Positives = 20/21 (95%)
Frame = -3 / +1
Query: 358 CRNSTRLQVVPLLASRSQALR 296
CRNSTRLQ++P LASR+QALR
Sbjct: 322 CRNSTRLQIIPHLASRAQALR 384
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 189,910,232
Number of Sequences: 369679
Number of extensions: 2682957
Number of successful extensions: 127716
Number of sequences better than 1.0e-020: 5
length of database: 67,802,888
effective HSP length: 49
effective length of database: 49,688,617
effective search space used: 3478203190
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)