TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE37h07.yg.2.1
(642 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, compl... 89 4e-042
gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZA... 100 6e-041
gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Po... 154 5e-037
gb|AI164628.1|AI164628 A066P21U Hybrid aspen plasmid librar... 136 2e-031
gb|CF232861.1|CF232861 PtaJXO0016F7F0711 Poplar cDNA librar... 87 9e-030
gb|CK099401.1|CK099401 A066P21.5pR Hybrid aspen plasmid lib... 119 9e-030
gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6... 125 2e-028
gb|AJ772088.1|AJ772088 AJ772088 Populus euphratica shoot in... 114 6e-025
gb|CK116341.1|CK116341 B010P30 Hybrid aspen plasmid library... 105 2e-022
>gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, complete sequence
Length = 96953
Score = 88.6 bits (187), Expect(4) = 4e-042
Identities = 32/53 (60%), Positives = 40/53 (75%)
Frame = -2 / +2
Query: 341 LEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQK 183
LEV +AY GG+ S+IL+T+SSWF+ +SWLFAPY+FNPSGFEWQK
Sbjct: 28280 LEVVLLLVVYLAYGYNDGGALSYILLTVSSWFMALSWLFAPYLFNPSGFEWQK 28438
Score = 62.5 bits (130), Expect(4) = 5e-022
Identities = 28/58 (48%), Positives = 35/58 (60%)
Frame = +1 / -3
Query: 169 KSSTVFCHSKPEGLKM*GAKSQDITRNQLLIVIRINEDEPPXXXXYAXXXIRRRATSS 342
++S FCHS P+GL *GAKSQD NQLL V R+ ++ PP YA + TSS
Sbjct: 28452 RNSAYFCHSNPDGLNK*GAKSQDRAINQLLTVRRM*DNAPPSLYPYARYTTSKSTTSS 28279
Score = 58.8 bits (122), Expect(4) = 4e-042
Identities = 23/29 (79%), Positives = 25/29 (86%)
Frame = -2 / +3
Query: 431 YRATXRGFVVXHIXFAENYRLYSXSHFVK 345
Y+AT RGFVV HI F+ENYRLYS SHFVK
Sbjct: 28110 YQATGRGFVVRHIRFSENYRLYSRSHFVK 28196
Score = 47.3 bits (97), Expect(4) = 4e-042
Identities = 21/40 (52%), Positives = 23/40 (57%)
Frame = -2 / +2
Query: 551 MXXVXXFITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAK 432
+ V FITMQLQ CSVF FSL T G ILH A+
Sbjct: 27902 LQAVVNFITMQLQLCSVFFTFSLGTKSHYFGRTILHGGAR 28021
Score = 33.6 bits (67), Expect(4) = 5e-022
Identities = 17/33 (51%), Positives = 20/33 (60%)
Frame = +1 / -3
Query: 445 *RIXLPX*XXXVXSEKXKNTEQXWSCIVIKXXT 543
*R+ LP * V +E K TEQ SCIV+K T
Sbjct: 28008 *RMVLPK*CDFVPNENVKKTEQS*SCIVMKLTT 27910
Score = 32.7 bits (65), Expect(4) = 4e-042
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -2 / +2
Query: 185 KTVEDFDDWTXWXXY 141
+TVEDF DWT W Y
Sbjct: 28514 RTVEDFSDWTNWLFY 28558
Score = 32.2 bits (64), Expect(4) = 5e-022
Identities = 18/29 (62%), Positives = 19/29 (65%)
Frame = +1 / -2
Query: 346 LTK*LXE*SL*FSANXMCXTTKPLXVARY 432
LTK L E*SL*FS N + TT P VA Y
Sbjct: 28195 LTKWLRE*SL*FSENLIWRTTNPRPVA*Y 28109
Score = 29.9 bits (59), Expect(4) = 5e-022
Identities = 18/33 (54%), Positives = 18/33 (54%)
Frame = +1 / -3
Query: 85 SSSHQDSQLXXXXXXXXXL*XXXXVQSSKSSTV 183
SSSHQ SQL L* VQS KSSTV
Sbjct: 28614 SSSHQASQLSCPLTPIPPL*KSQFVQSLKSSTV 28516
>gb|AI166267.1|AI166267 xylem.est.116 Poplar xylem Lambda ZAPII library Populus trichocarpa
cDNA 5', mRNA sequence
Length = 645
Score = 100 bits (212), Expect(2) = 6e-041
Identities = 46/101 (45%), Positives = 56/101 (55%)
Frame = -2 / +1
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q V G + M+M L + F+ MQLQ VF FSL T G
Sbjct: 73 ALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFLLMQLQLAPVFFTFSLGTKTHYYGR 252
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEV 333
+LH AKYR+T RGFVV H FA+NYRLYS SHFVK +E+
Sbjct: 253 TLLHGGAKYRSTGRGFVVFHAKFADNYRLYSRSHFVKGIEM 375
Score = 87.7 bits (185), Expect(2) = 6e-041
Identities = 28/43 (65%), Positives = 37/43 (86%)
Frame = -2 / +1
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
+++LITIS WF+V +WLFAP++FNPSGFEWQK V+D+ DW W
Sbjct: 430 AYVLITISMWFMVGTWLFAPFLFNPSGFEWQKIVDDWSDWNKW 558
Score = 60.2 bits (125), Expect(3) = 6e-024
Identities = 33/101 (32%), Positives = 46/101 (45%)
Frame = +3 / -1
Query: 321 QEESNLKRFDKVTXRIEPIIFSKXYVPXNKTSXSSTILCXXMKDXXTXXXXXCXKRKXXK 500
++ +L DKVT I+PI+ SK + +KTS S I C M+ + C KRK K
Sbjct: 387 KQNHHLNTLDKVTAGIKPIVVSKFGMEDHKTSTSGPIFCPTMQQCPSIVVGLCAKRKCEK 208
Query: 501 HRTXLELHCNKXXNXXHXXQXQNXXHYHRXXXXNTXLXQKL 623
+R L LH K Q H HR + L ++L
Sbjct: 207 YRC*L*LHQ*KFT*CSAETPFQANFHQHRQCHQKSYLNKRL 85
Score = 58.3 bits (121), Expect(3) = 6e-024
Identities = 26/37 (70%), Positives = 29/37 (78%)
Frame = +1 / -2
Query: 160 QSSKSSTVFCHSKPEGLKM*GAKSQDITRNQLLIVIR 270
QS +SST+FCHSKPEGLK GAKSQ T N + IVIR
Sbjct: 548 QSDQSSTIFCHSKPEGLKRNGAKSQVPTINHIDIVIR 438
Score = 29.5 bits (58), Expect(3) = 6e-024
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +1 / -2
Query: 76 CACSSSHQDSQL 111
C+CSSSH DSQL
Sbjct: 632 CSCSSSHHDSQL 597
>gb|BU896476.1|BU896476 X041D11 Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 467
Score = 154 bits (331), Expect = 5e-037
Identities = 68/149 (45%), Positives = 85/149 (57%)
Frame = -2 / +2
Query: 635 AXNAQFLVXXGIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGX 456
A +Q V G + M+M L + FI MQLQ VF FSL T G
Sbjct: 20 ALASQSFVQIGFLMALPMMMEIGLEKGFRNALSDFILMQLQLAPVFFTFSLGTKTHYYGR 199
Query: 455 XILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSS 276
+LH + YRAT RGFVV H FA+NYRLYS SHFVK +E+ + G +
Sbjct: 200 TLLHGGSAYRATGRGFVVFHAKFADNYRLYSRSHFVKGIELMILLLVFHIFGRSYRGVFA 379
Query: 275 FILITISSWFLVMSWLFAPYIFNPSGFEW 189
++LITIS WF+V +WLFAP++FNPSGFEW
Sbjct: 380 YVLITISMWFMVGTWLFAPFLFNPSGFEW 466
>gb|AI164628.1|AI164628 A066P21U Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA 5', mRNA sequence
Length = 268
Score = 136 bits (291), Expect = 2e-031
Identities = 50/77 (64%), Positives = 58/77 (75%)
Frame = -2 / +2
Query: 371 LYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYIFNPSGFE 192
LYS SHFVK LEV +AY GG+ S+IL+T+SSWF+ +SWLFAPY+FNPSGFE
Sbjct: 2 LYSRSHFVKGLEVVLLLVVYLAYGYNDGGALSYILLTVSSWFMALSWLFAPYLFNPSGFE 181
Query: 191 WQKTVEDFDDWTXWXXY 141
WQKTVEDF DWT W Y
Sbjct: 182 WQKTVEDFSDWTNWLFY 232
>gb|CF232861.1|CF232861 PtaJXO0016F7F0711 Poplar cDNA library from young opposite xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 737
Score = 86.8 bits (183), Expect(2) = 9e-030
Identities = 28/43 (65%), Positives = 37/43 (86%)
Frame = -2 / +2
Query: 278 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTXW 150
+++LITIS WF+V +WLFAP++FNPSGFEWQK V+D+ DW W
Sbjct: 158 AYLLITISMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKW 286
Score = 63.4 bits (132), Expect(2) = 9e-030
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = -2 / +2
Query: 434 KYRATXRGFVVXHIXFAENYRLYSXSHFVKALEV 333
KYR T RGFVV H FAENYRLYS SHFVK +E+
Sbjct: 2 KYRPTGRGFVVFHAKFAENYRLYSRSHFVKGIEM 103
>gb|CK099401.1|CK099401 A066P21.5pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A066P21 5', mRNA sequence
Length = 323
Score = 119 bits (254), Expect(2) = 9e-030
Identities = 44/70 (62%), Positives = 54/70 (77%)
Frame = -2 / +1
Query: 395 IXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPY 216
+ F+ENYRLYS SHFVK LEV +AY GG+ S+IL+T+SSWF+ +SWLFAPY
Sbjct: 70 LEFSENYRLYSRSHFVKGLEVVLLLVVYLAYGYNDGGALSYILLTVSSWFMALSWLFAPY 249
Query: 215 IFNPSGFEWQ 186
+FNPSGFEWQ
Sbjct: 250 LFNPSGFEWQ 279
Score = 30.4 bits (60), Expect(2) = 9e-030
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 188 QKTVEDFDDWTXW 150
++TVEDF DWT W
Sbjct: 278 KQTVEDFSDWTNW 316
>gb|AJ770061.1|AJ770061 AJ770061 Populus euphratica leaf 3-6 months, adult Populus
euphratica cDNA clone P0001100006E01F1, mRNA sequence
Length = 295
Score = 125 bits (268), Expect = 2e-028
Identities = 55/91 (60%), Positives = 61/91 (67%)
Frame = -2 / -1
Query: 605 GIXXXVXMIMGXILXLXXMXXVXXFITMQLQXCSVFXXFSLXTXXXXXGXXILHXXAKYR 426
GI V M++G IL L + V FITMQLQ CSVF FSL T G ILH A+Y+
Sbjct: 292 GIFTAVPMVLGFILELGFLRAVVSFITMQLQLCSVFFTFSLGTKSHYFGRTILHGGARYQ 113
Query: 425 ATXRGFVVXHIXFAENYRLYSXSHFVKALEV 333
AT RGFVV HI F+ENYRLYS SHFVK LEV
Sbjct: 112 ATGRGFVVRHIRFSENYRLYSRSHFVKGLEV 20
>gb|AJ772088.1|AJ772088 AJ772088 Populus euphratica shoot in vitro plantlet Israel Populus
euphratica cDNA clone P0001700001C10F1, mRNA sequence
Length = 341
Score = 114 bits (243), Expect = 6e-025
Identities = 47/97 (48%), Positives = 60/97 (61%)
Frame = -2 / -2
Query: 503 VFXXFSLXTXXXXXGXXILHXXAKYRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXX 324
VF FSL T G +LH + YRAT RGFVV H FA+NYRLYS SHFVK +E+
Sbjct: 292 VFFTFSLGTKTHYYGRTLLHGGSAYRATGRGFVVFHAKFADNYRLYSRSHFVKGIELMIL 113
Query: 323 XXXXIAYXXXXGGSSSFILITISSWFLVMSWLFAPYI 213
+ G +++LITIS WF+V +WLFAP++
Sbjct: 112 LLVFHIFGRSYRGVVAYVLITISMWFMVGTWLFAPFL 2
>gb|CK116341.1|CK116341 B010P30 Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone B010P30 5', mRNA sequence
Length = 297
Score = 105 bits (225), Expect = 2e-022
Identities = 42/83 (50%), Positives = 55/83 (66%)
Frame = -2 / +1
Query: 431 YRATXRGFVVXHIXFAENYRLYSXSHFVKALEVAXXXXXXIAYXXXXGGSSSFILITISS 252
YRAT GFVV H FA+NYRLYS SHFVK +E+ + G +++LITIS
Sbjct: 7 YRATGCGFVVFHAKFADNYRLYSRSHFVKGIELMILLLVFHIFGRSYRGVFAYVLITISM 186
Query: 251 WFLVMSWLFAPYIFNPSGFEWQK 183
WF+V +WLFAP++FNPSG ++
Sbjct: 187 WFMVGTWLFAPFLFNPSGLSGKR 255
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 179,024,062
Number of Sequences: 369679
Number of extensions: 1522549
Number of successful extensions: 15333
Number of sequences better than 1.0e-020: 10
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7929779958
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)