TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4573985.2.1
(373 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BP924653.1|BP924653 BP924653 full-length enriched poplar... 204 5e-052
gb|AY535003.1| Populus tremuloides endo-1,4-beta-glucanase ... 204 5e-052
gb|AY660967.1| Populus tremula x Populus tremuloides Cel9A ... 204 5e-052
gb|BU828319.1|BU828319 K020P84P Populus apical shoot cDNA l... 195 2e-049
gb|BI128164.1|BI128164 G071P89Y Populus cambium cDNA librar... 193 6e-049
gb|BI132085.1|BI132085 G130P88Y Populus cambium cDNA librar... 168 3e-041
gb|CV256150.1|CV256150 WS0243.B21_B03 PTxD-ICC-N-A-14 Popul... 105 1e-022
gb|BU896208.1|BU896208 X037C05 Populus wood cDNA library Po... 105 2e-022
gb|CA932657.1|CA932657 MTU5CS.P15.G09 Aspen stem cDNA Libra... 101 3e-021
gb|CA932943.1|CA932943 MTU5CS.P1.D12 Aspen stem cDNA Librar... 101 3e-021
>gb|BP924653.1|BP924653 BP924653 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-034_O04.f 5', mRNA sequence
Length = 553
Score = 204 bits (439), Expect = 5e-052
Identities = 82/111 (73%), Positives = 87/111 (78%)
Frame = -3 / +3
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMR 177
+YEA GEL+H+KELIKWG DY LKTFNSSADTIDRIVAQVG GDTS GS+ PNDHYCWMR
Sbjct: 111 KYEAAGELNHVKELIKWGADYFLKTFNSSADTIDRIVAQVGSGDTSGGSTTPNDHYCWMR 290
Query: 176 PEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
PEDIDY RPVTEC SCSD SIVFKD+K YS KLV GAK L
Sbjct: 291 PEDIDYDRPVTECSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAKTL 443
Score = 111 bits (237), Expect = 2e-024
Identities = 56/92 (60%), Positives = 61/92 (66%)
Frame = +1 / -1
Query: 79 TMEXXXXXXXXXXXXXEQE*HSVTGLL*SMSSGLIQQ*WSLG*LLPLEVSPTPT*ATILS 258
T++ EQE HSVTGL *SMSSG IQQ* S G +LP EVSP PT ATILS
Sbjct: 388 TIDAEAKAAAISAARSEQELHSVTGLS*SMSSGRIQQ**SFGVVLPPEVSPEPTCATILS 209
Query: 259 IVSADELKVLRM*SVPHLISSLIWSSSPMASY 354
+VSA+ L V R *S PH ISSL W SSP ASY
Sbjct: 208 MVSAELLNVFRK*SAPHFISSLTWLSSPAASY 113
>gb|AY535003.1| Populus tremuloides endo-1,4-beta-glucanase (Kor1) mRNA, complete cds
Length = 2562
Score = 204 bits (439), Expect = 5e-052
Identities = 82/111 (73%), Positives = 87/111 (78%)
Frame = -3 / +2
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMR 177
+YEA GEL+H+KELIKWG DY LKTFNSSADTIDRIVAQVG GDTS GS+ PNDHYCWMR
Sbjct: 833 KYEAAGELNHVKELIKWGADYFLKTFNSSADTIDRIVAQVGSGDTSGGSTTPNDHYCWMR 1012
Query: 176 PEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
PEDIDY RPVTEC SCSD SIVFKD+K YS KLV GAK L
Sbjct: 1013 PEDIDYDRPVTECSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAKTL 1165
Score = 113 bits (241), Expect = 6e-025
Identities = 57/92 (61%), Positives = 62/92 (67%)
Frame = +1 / -1
Query: 79 TMEXXXXXXXXXXXXXEQE*HSVTGLL*SMSSGLIQQ*WSLG*LLPLEVSPTPT*ATILS 258
T++ EQE HSVTGL *SMSSG IQQ* S G +LP EVSP PT ATILS
Sbjct: 1110 TIDAEAKAAAISAARSEQELHSVTGLS*SMSSGRIQQ**SFGVVLPPEVSPEPTCATILS 931
Query: 259 IVSADELKVLRM*SVPHLISSLIWSSSPMASY 354
+VSA+ L VLR *S PH ISSL W SSP ASY
Sbjct: 930 MVSAELLNVLRK*SAPHFISSLTWLSSPAASY 835
>gb|AY660967.1| Populus tremula x Populus tremuloides Cel9A mRNA, complete cds
Length = 1860
Score = 204 bits (439), Expect = 5e-052
Identities = 82/111 (73%), Positives = 87/111 (78%)
Frame = -3 / +1
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMR 177
+YEA GEL+H+KELIKWG DY LKTFNSSADTIDRIVAQVG GDTS GS+ PNDHYCWMR
Sbjct: 571 KYEAAGELNHVKELIKWGADYFLKTFNSSADTIDRIVAQVGSGDTSGGSTTPNDHYCWMR 750
Query: 176 PEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
PEDIDY RPVTEC SCSD SIVFKD+K YS KLV GAK L
Sbjct: 751 PEDIDYDRPVTECSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAKTL 903
Score = 113 bits (241), Expect = 6e-025
Identities = 57/92 (61%), Positives = 62/92 (67%)
Frame = +1 / -2
Query: 79 TMEXXXXXXXXXXXXXEQE*HSVTGLL*SMSSGLIQQ*WSLG*LLPLEVSPTPT*ATILS 258
T++ EQE HSVTGL *SMSSG IQQ* S G +LP EVSP PT ATILS
Sbjct: 848 TIDAEAKAAAISAARSEQELHSVTGLS*SMSSGRIQQ**SFGVVLPPEVSPEPTCATILS 669
Query: 259 IVSADELKVLRM*SVPHLISSLIWSSSPMASY 354
+VSA+ L VLR *S PH ISSL W SSP ASY
Sbjct: 668 MVSAELLNVLRK*SAPHFISSLTWLSSPAASY 573
>gb|BU828319.1|BU828319 K020P84P Populus apical shoot cDNA library Populus tremula x
Populus tremuloides cDNA 5 prime, mRNA sequence
Length = 603
Score = 195 bits (421), Expect = 2e-049
Identities = 78/105 (74%), Positives = 83/105 (79%)
Frame = -3 / +3
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMR 177
+YEA GEL+H+KELIKWG DY LKTFNSSADTIDRIVAQVG GDTS GS+ PNDHYCWMR
Sbjct: 15 KYEAAGELNHVKELIKWGADYFLKTFNSSADTIDRIVAQVGSGDTSGGSTTPNDHYCWMR 194
Query: 176 PEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLV 42
PEDIDY RPVTEC SCSD SIVFKD+K YS KLV
Sbjct: 195 PEDIDYDRPVTECSSCSDLAAEMAAALASASIVFKDNKAYSQKLV 329
Score = 113 bits (241), Expect = 6e-025
Identities = 57/92 (61%), Positives = 62/92 (67%)
Frame = +1 / -3
Query: 79 TMEXXXXXXXXXXXXXEQE*HSVTGLL*SMSSGLIQQ*WSLG*LLPLEVSPTPT*ATILS 258
T++ EQE HSVTGL *SMSSG IQQ* S G +LP EVSP PT ATILS
Sbjct: 292 TIDAEAKAAAISAARSEQELHSVTGLS*SMSSGRIQQ**SFGVVLPPEVSPEPTCATILS 113
Query: 259 IVSADELKVLRM*SVPHLISSLIWSSSPMASY 354
+VSA+ L VLR *S PH ISSL W SSP ASY
Sbjct: 112 MVSAELLNVLRK*SAPHFISSLTWLSSPAASY 17
>gb|BI128164.1|BI128164 G071P89Y Populus cambium cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 467
Score = 193 bits (417), Expect = 6e-049
Identities = 79/108 (73%), Positives = 83/108 (76%)
Frame = -3 / +2
Query: 347 AIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMRPED 168
A GEL+ +KELIKWG DY LKTFNSSADTIDRIVAQVG GDTS GS+ PNDHYCWMRPED
Sbjct: 2 AAGELNRVKELIKWGADYFLKTFNSSADTIDRIVAQVGSGDTSGGSTTPNDHYCWMRPED 181
Query: 167 IDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
IDY RPVTEC SCSD SIVFKD+K YS KLV GAK L
Sbjct: 182 IDYDRPVTECSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAKTL 325
Score = 101 bits (216), Expect = 2e-021
Identities = 54/90 (60%), Positives = 59/90 (65%)
Frame = +1 / -3
Query: 79 TMEXXXXXXXXXXXXXEQE*HSVTGLL*SMSSGLIQQ*WSLG*LLPLEVSPTPT*ATILS 258
T++ EQE HSVTGL *SMSSG IQQ* S G +LP EVSP PT ATILS
Sbjct: 270 TIDAEAKAAAISAARSEQELHSVTGLS*SMSSGRIQQ**SFGVVLPPEVSPEPTCATILS 91
Query: 259 IVSADELKVLRM*SVPHLISSLIWSSSPMA 348
+VSA+ L VLR *S PH ISSL SSP A
Sbjct: 90 MVSAELLNVLRK*SAPHFISSLTRLSSPAA 1
>gb|BI132085.1|BI132085 G130P88Y Populus cambium cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 291
Score = 168 bits (361), Expect = 3e-041
Identities = 63/78 (80%), Positives = 69/78 (88%)
Frame = -3 / +2
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKGSSQPNDHYCWMR 177
+YEA GEL+H+KELIKWG DY LKTFNSSADT+DRI AQVG GDT+ GS+ PNDHYCWMR
Sbjct: 44 KYEAAGELNHVKELIKWGADYFLKTFNSSADTVDRIFAQVGSGDTAGGSTTPNDHYCWMR 223
Query: 176 PEDIDYKRPVTECHSCSD 123
PEDIDY RPVTEC SCSD
Sbjct: 224 PEDIDYDRPVTECSSCSD 277
Score = 105 bits (224), Expect = 1e-022
Identities = 54/76 (71%), Positives = 56/76 (73%)
Frame = +1 / -1
Query: 127 EQE*HSVTGLL*SMSSGLIQQ*WSLG*LLPLEVSPTPT*ATILSIVSADELKVLRM*SVP 306
EQE HSVTGL *SMSSG IQQ* S G +LP VSP PT A ILS VSA+ L VLR *S P
Sbjct: 273 EQELHSVTGLS*SMSSGRIQQ**SFGVVLPPAVSPEPTCANILSTVSAELLNVLRK*SAP 94
Query: 307 HLISSLIWSSSPMASY 354
H ISSL W SSP ASY
Sbjct: 93 HFISSLTWLSSPAASY 46
>gb|CV256150.1|CV256150 WS0243.B21_B03 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0243_B03 3', mRNA sequence
Length = 500
Score = 105 bits (225), Expect = 1e-022
Identities = 42/64 (65%), Positives = 44/64 (68%)
Frame = -3 / -2
Query: 215 GSSQPNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKG 36
GS+ NDHYCWMRPEDIDY RPVTEC SCSD SIVFKD+K YS KLV G
Sbjct: 499 GSTTQNDHYCWMRPEDIDYVRPVTECSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHG 320
Query: 35 AKAL 24
AK L
Sbjct: 319 AKTL 308
>gb|BU896208.1|BU896208 X037C05 Populus wood cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 549
Score = 105 bits (224), Expect = 2e-022
Identities = 41/60 (68%), Positives = 42/60 (70%)
Frame = -3 / +2
Query: 203 PNDHYCWMRPEDIDYKRPVTECHSCSDXXXXXXXXXXXXSIVFKDSKTYSDKLVKGAKAL 24
PNDHYCWMRPEDIDY RPVTEC SCSD SIVFKD+K YS KLV GAK L
Sbjct: 5 PNDHYCWMRPEDIDYDRPVTECSSCSDLAAEMAAALASASIVFKDNKAYSQKLVHGAKTL 184
>gb|CA932657.1|CA932657 MTU5CS.P15.G09 Aspen stem cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 303
Score = 101 bits (215), Expect = 3e-021
Identities = 40/48 (83%), Positives = 44/48 (91%)
Frame = -3 / -3
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKG 213
+YEA+GEL+H+KELIKWG DY LKTFNSSADTIDRIVAQVG GDTS G
Sbjct: 145 KYEAVGELNHVKELIKWGADYFLKTFNSSADTIDRIVAQVGSGDTSGG 2
>gb|CA932943.1|CA932943 MTU5CS.P1.D12 Aspen stem cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 300
Score = 101 bits (215), Expect = 3e-021
Identities = 40/48 (83%), Positives = 44/48 (91%)
Frame = -3 / -3
Query: 356 RYEAIGELDHIKELIKWGTDYILKTFNSSADTIDRIVAQVGVGDTSKG 213
+YEA+GEL+H+KELIKWG DY LKTFNSSADTIDRIVAQVG GDTS G
Sbjct: 145 KYEAVGELNHVKELIKWGADYFLKTFNSSADTIDRIVAQVGSGDTSGG 2
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 165,144,427
Number of Sequences: 369679
Number of extensions: 2041575
Number of successful extensions: 89488
Number of sequences better than 1.0e-020: 11
length of database: 67,802,888
effective HSP length: 49
effective length of database: 49,688,617
effective search space used: 3676957658
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)