TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665525.2.1
(620 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV255308.1|CV255308 WS02412.B21_K12 PTxD-ICC-N-A-14 Popu... 233 8e-061
gb|DT517578.1|DT517578 WS02434.B21_L09 PTxD-ICC-N-A-14 Popu... 233 8e-061
gb|CK096470.1|CK096470 UB15CPF09.3pR Populus active cambium... 230 8e-060
gb|CX655011.1|CX655011 PO01003G06 Poplar SC cDNA library Po... 230 8e-060
gb|CK092473.1|CK092473 G084P78.3pR Populus tension wood cDN... 207 4e-053
gb|CA931213.1|CA931213 MTU2TA.P4.A06 Aspen apex cDNA Librar... 149 1e-035
gb|CA931239.1|CA931239 MTU2TA.P4.E07 Aspen apex cDNA Librar... 149 1e-035
gb|CX655147.1|CX655147 PO02030E06 Poplar SC cDNA library Po... 142 2e-033
>gb|CV255308.1|CV255308 WS02412.B21_K12 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02412_K12 3', mRNA sequence
Length = 736
Score = 233 bits (503), Expect = 8e-061
Identities = 87/129 (67%), Positives = 104/129 (80%)
Frame = -2 / -2
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
+GC+KKGPVFTDPKLKWYEP S+LLG EYFLHAYGPIY LRNNSFRMFS
Sbjct: 669 LGCLKKGPVFTDPKLKWYEPLSYLLGKEYFLHAYGPIYALSADVVASLVALRNNSFRMFS 490
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIG+W+LAMNVNHE+ ALC P+CT SS+AVWDIPKCSGLC+PE ++LELHQ++ C
Sbjct: 489 NEDVTIGAWILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEARLLELHQQESC 310
Query: 259 TGGPTVAAE 233
+ PT+ ++
Sbjct: 309 SKSPTMESD 283
Score = 140 bits (301), Expect = 6e-033
Identities = 64/107 (59%), Positives = 68/107 (63%)
Frame = +3 / +2
Query: 297 SGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIASIHDPIVTS*ILNIRXXXXXXXX 476
SG SPEH GMSQTA +D V SG SA + S LTFIA IH PIVTS +LNIR
Sbjct: 347 SGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPIVTSSLLNIRKLLFLRAT 526
Query: 477 XXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPFFMHP 617
IGPYA*RKYS PSK D GSYH + GSVKTGPFF HP
Sbjct: 527 KLATTSAERAYIGPYA*RKYSFPSK*DSGSYHLSFGSVKTGPFFKHP 667
>gb|DT517578.1|DT517578 WS02434.B21_L09 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02434_L09 3', mRNA sequence
Length = 911
Score = 233 bits (503), Expect = 8e-061
Identities = 87/129 (67%), Positives = 104/129 (80%)
Frame = -2 / -1
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
+GC+KKGPVFTDPKLKWYEP S+LLG EYFLHAYGPIY LRNNSFRMFS
Sbjct: 686 LGCLKKGPVFTDPKLKWYEPLSYLLGKEYFLHAYGPIYALSADVVASLVALRNNSFRMFS 507
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIG+W+LAMNVNHE+ ALC P+CT SS+AVWDIPKCSGLC+PE ++LELHQ++ C
Sbjct: 506 NEDVTIGAWILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEARLLELHQQESC 327
Query: 259 TGGPTVAAE 233
+ PT+ ++
Sbjct: 326 SKSPTMESD 300
Score = 140 bits (301), Expect = 6e-033
Identities = 64/107 (59%), Positives = 68/107 (63%)
Frame = +3 / +1
Query: 297 SGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIASIHDPIVTS*ILNIRXXXXXXXX 476
SG SPEH GMSQTA +D V SG SA + S LTFIA IH PIVTS +LNIR
Sbjct: 364 SGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPIVTSSLLNIRKLLFLRAT 543
Query: 477 XXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPFFMHP 617
IGPYA*RKYS PSK D GSYH + GSVKTGPFF HP
Sbjct: 544 KLATTSAERAYIGPYA*RKYSFPSK*DSGSYHLSFGSVKTGPFFKHP 684
>gb|CK096470.1|CK096470 UB15CPF09.3pR Populus active cambium cDNA library Populus tremula
cDNA clone UB15CPF09 3', mRNA sequence
Length = 774
Score = 230 bits (496), Expect = 8e-060
Identities = 86/129 (66%), Positives = 105/129 (81%)
Frame = -2 / -3
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
+GC+KKGPVFTDPKLKWYEP S+LLG+EYFLHAYGPIY LR+NSFRMFS
Sbjct: 532 LGCLKKGPVFTDPKLKWYEPLSYLLGTEYFLHAYGPIYALSADVVASLVALRDNSFRMFS 353
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIG+W+LAMNVNHE+ ALC P+CT SS+AVWDIPKCSGLC+PE ++LELHQ++ C
Sbjct: 352 NEDVTIGAWILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEDRLLELHQQESC 173
Query: 259 TGGPTVAAE 233
+ PT+ ++
Sbjct: 172 SKNPTMESD 146
Score = 141 bits (303), Expect = 3e-033
Identities = 64/108 (59%), Positives = 69/108 (63%)
Frame = +3 / +3
Query: 294 TSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIASIHDPIVTS*ILNIRXXXXXXX 473
+SG SPEH GMSQTA +D V SG SA + S LTFIA IH PIVTS +LNIR
Sbjct: 207 SSGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPIVTSSLLNIRKLLSLRA 386
Query: 474 XXXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPFFMHP 617
IGPYA*RKYS PSK D GSYH + GSVKTGPFF HP
Sbjct: 387 TKLATTSAERAYIGPYA*RKYSVPSK*DSGSYHLSFGSVKTGPFFKHP 530
>gb|CX655011.1|CX655011 PO01003G06 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO01003G06 5', mRNA sequence
Length = 693
Score = 230 bits (496), Expect = 8e-060
Identities = 86/129 (66%), Positives = 105/129 (81%)
Frame = -2 / +3
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
+GC+KKGPVFTDPKLKWYEP S+LLG+EYFLHAYGPIY LR+NSFRMFS
Sbjct: 234 LGCLKKGPVFTDPKLKWYEPLSYLLGTEYFLHAYGPIYALSADVVASLVALRDNSFRMFS 413
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIG+W+LAMNVNHE+ ALC P+CT SS+AVWDIPKCSGLC+PE ++LELHQ++ C
Sbjct: 414 NEDVTIGAWILAMNVNHEDNRALCSPECTPSSIAVWDIPKCSGLCNPEDRLLELHQQESC 593
Query: 259 TGGPTVAAE 233
+ PT+ ++
Sbjct: 594 SKNPTMESD 620
Score = 141 bits (303), Expect = 3e-033
Identities = 64/108 (59%), Positives = 69/108 (63%)
Frame = +3 / -3
Query: 294 TSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIASIHDPIVTS*ILNIRXXXXXXX 473
+SG SPEH GMSQTA +D V SG SA + S LTFIA IH PIVTS +LNIR
Sbjct: 559 SSGLQSPEHLGMSQTAIDDGVHSGEHSALLSS*LTFIARIHAPIVTSSLLNIRKLLSLRA 380
Query: 474 XXXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPFFMHP 617
IGPYA*RKYS PSK D GSYH + GSVKTGPFF HP
Sbjct: 379 TKLATTSAERAYIGPYA*RKYSVPSK*DSGSYHLSFGSVKTGPFFKHP 236
>gb|CK092473.1|CK092473 G084P78.3pR Populus tension wood cDNA library Populus tremula x
Populus tremuloides cDNA clone G084P78 3', mRNA sequence
Length = 726
Score = 207 bits (447), Expect = 4e-053
Identities = 79/126 (62%), Positives = 92/126 (73%)
Frame = -2 / -3
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPV TDPK+KWYE L+G+EYFLHAYGPIY RNNS RMFS
Sbjct: 613 IGCMKKGPVITDPKMKWYETSGHLIGNEYFLHAYGPIYVLSAEVVASLASARNNSLRMFS 434
Query: 439 I*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLELHQRKEC 260
DVTIGSWMLAMNV HE+ A+C+P CT +S+AVWDIPKCSGLC+P +M ELH+ C
Sbjct: 433 NEDVTIGSWMLAMNVYHEDNRAICDPRCTPTSIAVWDIPKCSGLCNPASRMKELHKIDMC 254
Query: 259 TGGPTV 242
+ PT+
Sbjct: 253 SKSPTL 236
Score = 116 bits (248), Expect = 1e-025
Identities = 60/130 (46%), Positives = 71/130 (54%)
Frame = +3 / +3
Query: 231 TSAATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIAS 410
+S +VG H + I +G H PEH G+SQTA + V GS A + S TFIAS
Sbjct: 225 SSGGSVGLLEHISIL*SSFIRLAGLHKPEHLGISQTAIDVGVHRGSHMARLSS**TFIAS 404
Query: 411 IHDPIVTS*ILNIRXXXXXXXXXXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSV 590
IHDP+VTS +LNI IGP A RKYS P + SYHF LGSV
Sbjct: 405 IHDPMVTSSLLNILKLLFLADANEATTSAERTYIGP*ACRKYSFPIRCPDVSYHFILGSV 584
Query: 591 KTGPFFMHPM 620
TGPFFMHP+
Sbjct: 585 ITGPFFMHPI 614
>gb|CA931213.1|CA931213 MTU2TA.P4.A06 Aspen apex cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 376
Score = 149 bits (320), Expect = 1e-035
Identities = 58/97 (59%), Positives = 68/97 (70%)
Frame = -2 / -1
Query: 532 FLHAYGPIYXXXXXXXXXXXXLRNNSFRMFSI*DVTIGSWMLAMNVNHENTHALCEPDCT 353
FLHAYGPIY RNNS RMFS DVTIGSWMLAMNV HE+ A+C+P CT
Sbjct: 376 FLHAYGPIYVLSAEVVASLASARNNSLRMFSNEDVTIGSWMLAMNVYHEDNRAICDPRCT 197
Query: 352 ESSVAVWDIPKCSGLCHPEVKMLELHQRKECTGGPTV 242
+S+AVWDIPKCSGLC+P +M ELH+ C+ PT+
Sbjct: 196 PTSIAVWDIPKCSGLCNPASRMKELHKIDMCSKSPTL 86
>gb|CA931239.1|CA931239 MTU2TA.P4.E07 Aspen apex cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 376
Score = 149 bits (320), Expect = 1e-035
Identities = 58/97 (59%), Positives = 68/97 (70%)
Frame = -2 / -1
Query: 532 FLHAYGPIYXXXXXXXXXXXXLRNNSFRMFSI*DVTIGSWMLAMNVNHENTHALCEPDCT 353
FLHAYGPIY RNNS RMFS DVTIGSWMLAMNV HE+ A+C+P CT
Sbjct: 376 FLHAYGPIYVLSAEVVASLASARNNSLRMFSNEDVTIGSWMLAMNVYHEDNRAICDPRCT 197
Query: 352 ESSVAVWDIPKCSGLCHPEVKMLELHQRKECTGGPTV 242
+S+AVWDIPKCSGLC+P +M ELH+ C+ PT+
Sbjct: 196 PTSIAVWDIPKCSGLCNPASRMKELHKIDMCSKSPTL 86
>gb|CX655147.1|CX655147 PO02030E06 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO02030E06 5', mRNA sequence
Length = 600
Score = 142 bits (305), Expect = 2e-033
Identities = 57/79 (72%), Positives = 61/79 (77%)
Frame = -2 / +3
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
+GCMKKGPVFTDPKLKWYEP S+LLG EYF HAYGPIY LRNNSFRM S
Sbjct: 363 LGCMKKGPVFTDPKLKWYEPLSYLLGKEYFYHAYGPIYALSADVVASLVVLRNNSFRMSS 542
Query: 439 I*DVTIGSWMLAMNVNHEN 383
DVTIG+WMLAMNVNHE+
Sbjct: 543 NEDVTIGAWMLAMNVNHED 599
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 331,657,806
Number of Sequences: 369679
Number of extensions: 4672573
Number of successful extensions: 52060
Number of sequences better than 1.0e-020: 8
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7587135145
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)