TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3276201.2.1
(1827 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY935501.1| Populus tremula x Populus tremuloides putati... 166 2e-054
gb|CF235078.1|CF235078 PtaJXT0018F2F0212 Poplar cDNA librar... 102 1e-035
gb|AJ769909.1|AJ769909 AJ769909 Populus euphratica leaf 3-6... 68 4e-031
gb|AI166598.1|AI166598 xylem.est.414 Poplar xylem Lambda ZA... 133 3e-030
gb|AI162606.1|AI162606 A020P21U Hybrid aspen plasmid librar... 129 6e-029
gb|CV268607.1|CV268607 WS0205.B21_P08 PTxN-IB-N-A-11 Populu... 102 2e-027
gb|CF234196.1|CF234196 PtaJXO3F6F0612 Poplar cDNA library f... 63 5e-025
gb|CV272355.1|CV272355 WS0157.B21_G15 PTxN-IB-A-6 Populus t... 106 3e-022
>gb|AY935501.1| Populus tremula x Populus tremuloides putative endo-1,4-beta-xylanase
mRNA, complete cds
Length = 3001
Score = 166 bits (356), Expect(3) = 2e-054
Identities = 71/216 (32%), Positives = 119/216 (55%)
Frame = +2 / +1
Query: 281 APVDIWVDSVSLQPFTFDEWDGHTRRSADKVRRRTVKGGAMGANDKPMAHANVSIELLRL 460
A VD+ + + + P + H RR DK+R+R V G + + ++ +
Sbjct: 1618 AGVDLMLAGLQIFPVDRESRFKHLRRQTDKIRKRDVTLKFSGGGSSSVLGTFIKVKQTQN 1797
Query: 461 GFPFGNAITKEILGLPAYEKWFTSRFSVATFENEMKWYSTEWTQNHEDYRVPDAMMNLMR 640
FPFG+ +++ L + +F F+ A F NE+KWY TE Q + +Y D M++L +
Sbjct: 1798 SFPFGSCMSRMNLDNEDFVNFFVKNFNWAVFGNELKWYWTEAQQGNFNYSDADEMLDLCK 1977
Query: 641 KYKIKVRGHNVFWDDQNSQMQWVKPLNLAQLKAAMQKRLKSVVSPYAGKVIHWDVVNENL 820
K I+ RGH +FW+ + QW+K LN + A+Q RL +++ Y GK H+DV NE L
Sbjct: 1978 KNNIEARGHCIFWEVDGTVQQWIKALNKNDMMTAVQNRLTGLLTRYKGKFSHYDVNNEML 2157
Query: 821 HFNFFETKLGPMASAQIYQQVGQLDRNAILFMNEFN 928
H +F++ LG A +++ QLD +A+LF+N+++
Sbjct: 2158 HGSFYQDHLGKDIRANMFKTANQLDPSALLFVNDYH 2265
Score = 45.5 bits (93), Expect(3) = 2e-054
Identities = 17/37 (45%), Positives = 25/37 (67%)
Frame = +2 / +1
Query: 1028 GVGLESHFSTPNIPYMRSSLDTLAKLKLPMWLTEVDV 1138
G+G++ H +P P + S+LD L L LP+W TE+DV
Sbjct: 2353 GIGIQGHIDSPVGPVVCSALDKLGILGLPIWFTELDV 2463
Score = 41.4 bits (84), Expect(3) = 2e-054
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +2 / +1
Query: 1166 LEQVLREGFAHPNVDGIVMWAAW 1234
LE +LRE +AHP VDG+++W W
Sbjct: 2497 LEVMLREAYAHPAVDGVMLWGFW 2565
>gb|CF235078.1|CF235078 PtaJXT0018F2F0212 Poplar cDNA library from young tension xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 662
Score = 102 bits (218), Expect(3) = 1e-035
Identities = 39/102 (38%), Positives = 65/102 (63%)
Frame = +2 / +1
Query: 623 MMNLMRKYKIKVRGHNVFWDDQNSQMQWVKPLNLAQLKAAMQKRLKSVVSPYAGKVIHWD 802
M++L +K I+ RGH +FW+ + QW+K LN + A+Q RL +++ Y GK H+D
Sbjct: 7 MLDLCKKNNIEARGHCIFWEVDGTVQQWIKALNKNDMMTAVQNRLTGLLTRYKGKFSHYD 186
Query: 803 VVNENLHFNFFETKLGPMASAQIYQQVGQLDRNAILFMNEFN 928
V NE LH +F++ LG A +++ QLD +A+LF+N+++
Sbjct: 187 VNNEMLHGSFYQDHLGKDIRANMFKTANQLDPSALLFVNDYH 312
Score = 45.5 bits (93), Expect(3) = 1e-035
Identities = 17/37 (45%), Positives = 25/37 (67%)
Frame = +2 / +1
Query: 1028 GVGLESHFSTPNIPYMRSSLDTLAKLKLPMWLTEVDV 1138
G+G++ H +P P + S+LD L L LP+W TE+DV
Sbjct: 400 GIGIQGHIDSPVGPVVCSALDKLGILGLPIWFTELDV 510
Score = 41.4 bits (84), Expect(3) = 1e-035
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +2 / +1
Query: 1166 LEQVLREGFAHPNVDGIVMWAAW 1234
LE +LRE +AHP VDG+++W W
Sbjct: 544 LEVMLREAYAHPAVDGVMLWGFW 612
>gb|AJ769909.1|AJ769909 AJ769909 Populus euphratica leaf 3-6 months, adult Populus euphratica
cDNA clone P0001100004D06F1, mRNA sequence
Length = 679
Score = 68.4 bits (143), Expect(4) = 4e-031
Identities = 28/51 (54%), Positives = 35/51 (68%)
Frame = +2 / -1
Query: 986 NQIRGYAGNGGLKLGVGLESHFSTPNIPYMRSSLDTLAKLKLPMWLTEVDV 1138
++IR G G+GLES F PN+P MR+ LD LA LKLP+WLTEVD+
Sbjct: 679 DKIRELERGGSSMNGIGLESRFXKPNLPLMRAILDKLATLKLPIWLTEVDI 527
Score = 56.5 bits (117), Expect(4) = 4e-031
Identities = 21/31 (67%), Positives = 24/31 (77%)
Frame = +2 / -1
Query: 1154 QVKYLEQVLREGFAHPNVDGIVMWAAWHARG 1246
Q YLEQVLREGF+HP VDGI++W A H G
Sbjct: 502 QATYLEQVLREGFSHPAVDGIMLWTAIHPNG 410
Score = 41.4 bits (84), Expect(4) = 4e-031
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +2 / -3
Query: 1295 GDLVDKLIAEWKTHRASATTDHNGAVELHLPLGDFKFTVSH 1417
GD VDKL+ EW+T + TD +G+ LG++K V +
Sbjct: 356 GDTVDKLLREWETGQGKGRTDDHGSYSFFGFLGEYKIMVQY 234
Score = 25.4 bits (49), Expect(4) = 4e-031
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2 / -1
Query: 1244 GCYVMCLTD 1270
GCY MCLTD
Sbjct: 409 GCYQMCLTD 383
>gb|AI166598.1|AI166598 xylem.est.414 Poplar xylem Lambda ZAPII library Populus trichocarpa
cDNA 5', mRNA sequence
Length = 542
Score = 133 bits (286), Expect = 3e-030
Identities = 53/139 (38%), Positives = 85/139 (61%)
Frame = +2 / +1
Query: 512 YEKWFTSRFSVATFENEMKWYSTEWTQNHEDYRVPDAMMNLMRKYKIKVRGHNVFWDDQN 691
+ +F F+ A F NE+KWY TE Q + +Y D M++L +K I+ RGH +FW+
Sbjct: 22 FVNFFVKNFNWAVFGNELKWYWTEPQQGNFNYSDADEMLDLCKKNNIEARGHCIFWEVDG 201
Query: 692 SQMQWVKPLNLAQLKAAMQKRLKSVVSPYAGKVIHWDVVNENLHFNFFETKLGPMASAQI 871
+ QW+K LN + A+Q RL +++ Y GK H+DV NE LH +F++ LG A +
Sbjct: 202 TVQQWIKALNKNDMMTAVQNRLTGLLTRYTGKFRHYDVNNEMLHGSFYQDHLGKDIRANM 381
Query: 872 YQQVGQLDRNAILFMNEFN 928
++ QLD +A+LF+N+++
Sbjct: 382 FKTANQLDPSAMLFVNDYH 438
>gb|AI162606.1|AI162606 A020P21U Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA 5', mRNA sequence
Length = 545
Score = 129 bits (276), Expect = 6e-029
Identities = 54/156 (34%), Positives = 85/156 (54%)
Frame = +2 / +2
Query: 347 HTRRSADKVRRRTVKGGAMGANDKPMAHANVSIELLRLGFPFGNAITKEILGLPAYEKWF 526
H RR DK+R+R V G + + ++ + FPFG+ +++ L + +F
Sbjct: 35 HLRRQTDKIRKRDVTLKFSGGGSSSVLGTFIKVKQTQNSFPFGSCMSRTNLDNEDFVNFF 214
Query: 527 TSRFSVATFENEMKWYSTEWTQNHEDYRVPDAMMNLMRKYKIKVRGHNVFWDDQNSQMQW 706
F+ A F NE+KWY TE Q + +Y D M++L +K I+ RGH +FW+ + QW
Sbjct: 215 VKNFNWAVFGNELKWYWTEAQQGNFNYSDADEMLDLCKKNNIEARGHCIFWEVDGTVQQW 394
Query: 707 VKPLNLAQLKAAMQKRLKSVVSPYAGKVIHWDVVNE 814
+K LN + A+Q RL +++ Y GK H+DV NE
Sbjct: 395 IKALNKNDMMTAVQNRLTGLLTRYKGKSSHYDVNNE 502
>gb|CV268607.1|CV268607 WS0205.B21_P08 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS0205_P08 3', mRNA sequence
Length = 592
Score = 102 bits (218), Expect(2) = 2e-027
Identities = 42/88 (47%), Positives = 57/88 (64%)
Frame = +2 / -1
Query: 1154 QVKYLEQVLREGFAHPNVDGIVMWAAWHARGCYVMCLTDNSFKNLPVGDLVDKLIAEWKT 1333
Q YLEQVLREG +H VDGI++W A H GCY M TD +F+N+ GD VDK EW+T
Sbjct: 481 QAIYLEQVLREGLSHLAVDGIMLWHAIHHNGCYQMDPTDCNFQNVSAGDTVDKFFKEWET 302
Query: 1334 HRASATTDHNGAVELHLPLGDFKFTVSH 1417
A+ TD++G+ L LG+++ V +
Sbjct: 301 GEANGLTDYHGSYRLFGLLGEYRIRVQY 218
Score = 41.4 bits (84), Expect(2) = 2e-027
Identities = 15/27 (55%), Positives = 21/27 (77%)
Frame = +2 / -1
Query: 1058 PNIPYMRSSLDTLAKLKLPMWLTEVDV 1138
PN+P MR+ +D L +KLP+ LTEVD+
Sbjct: 586 PNVPIMRAIIDKLGGIKLPICLTEVDI 506
>gb|CF234196.1|CF234196 PtaJXO3F6F0612 Poplar cDNA library from young opposite xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 565
Score = 63.4 bits (132), Expect(3) = 5e-025
Identities = 25/66 (37%), Positives = 43/66 (65%)
Frame = +2 / +2
Query: 731 LKAAMQKRLKSVVSPYAGKVIHWDVVNENLHFNFFETKLGPMASAQIYQQVGQLDRNAIL 910
+ A+Q RL +++ Y GK H+DV NE LH +F++ LG A +++ QLD +A+L
Sbjct: 119 MMTAVQNRLTGLLTRYKGKFSHYDVNNEMLHGSFYQDHLGKDIRANMFKTAKQLDPSALL 298
Query: 911 FMNEFN 928
F+N+++
Sbjct: 299 FVNDYH 316
Score = 45.5 bits (93), Expect(3) = 5e-025
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +2 / +3
Query: 617 DAMMNLMRKYKIKVRGHNVFWDDQNSQMQWVKPLN 721
D M++L +K I+ RGH +FW+ + QW+K LN
Sbjct: 6 DEMLDLCKKNNIEARGHCIFWEVDGTVQQWIKALN 110
Score = 44.6 bits (91), Expect(3) = 5e-025
Identities = 16/37 (43%), Positives = 26/37 (70%)
Frame = +2 / +2
Query: 1028 GVGLESHFSTPNIPYMRSSLDTLAKLKLPMWLTEVDV 1138
G+G++ H +P P + S+LD L L LP+W+T++DV
Sbjct: 404 GIGIQGHIXSPVGPVVCSALDKLGILGLPIWVTDLDV 514
>gb|CV272355.1|CV272355 WS0157.B21_G15 PTxN-IB-A-6 Populus trichocarpa x Populus nigra cDNA
clone WS0157_G15 3', mRNA sequence
Length = 395
Score = 106 bits (227), Expect = 3e-022
Identities = 39/78 (50%), Positives = 54/78 (69%)
Frame = +2 / -1
Query: 1184 EGFAHPNVDGIVMWAAWHARGCYVMCLTDNSFKNLPVGDLVDKLIAEWKTHRASATTDHN 1363
EGF+HP VDGI++W A H GCY MCLTD++ +NLP GD VDKL+ EW+T A+ TD +
Sbjct: 392 EGFSHPAVDGIMLWTAIHPNGCYQMCLTDSNLQNLPAGDTVDKLLKEWETGEANGLTDDH 213
Query: 1364 GAVELHLPLGDFKFTVSH 1417
G+ LG+++ V +
Sbjct: 212 GSYSFFGFLGEYRIRVQY 159
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 850,380,630
Number of Sequences: 369679
Number of extensions: 11607838
Number of successful extensions: 70699
Number of sequences better than 1.0e-020: 11
length of database: 67,802,888
effective HSP length: 54
effective length of database: 47,840,222
effective search space used: 26503482988
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)