TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204486.2.1
(607 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT489528.1|DT489528 WS02542.B21_H16 PT-MB-N-A-15 Populus... 170 6e-042
gb|DN499218.1|DN499218 T035A05.5pR Populus shoot meristem c... 165 2e-040
gb|DT504437.1|DT504437 WS01313.B21_F23 PTxD-IL-FL-A-4 Popul... 154 4e-037
gb|CX167586.1|CX167586 F09_69-15_11.ab1 leaf inoculated wit... 147 5e-035
gb|AJ771406.1|AJ771406 AJ771406 Populus euphratica leaf 3-6... 131 3e-030
gb|DN488268.1|DN488268 R045E02.3pR Populus root cDNA librar... 129 2e-029
gb|CF237321.1|CF237321 Ptajxtjxt4F7F0711 Poplar cDNA librar... 126 7e-029
gb|CV243431.1|CV243431 WS02518.B21_N18 PT-MB-N-A-15 Populus... 115 2e-025
gb|BU886438.1|BU886438 R045E02 Populus root cDNA library Po... 111 4e-024
gb|CV277864.1|CV277864 WS0144.B21_M12 PTxD-IL-A-5 Populus t... 108 4e-023
gb|CV266784.1|CV266784 WS0203.B21_P14 PTxN-IB-N-A-11 Populu... 107 5e-023
gb|CK318837.1|CK318837 X9P03h07 Populus stem seasonal libra... 105 2e-022
gb|DN497951.1|DN497951 R045E02.5pR Populus root cDNA librar... 87 2e-021
>gb|DT489528.1|DT489528 WS02542.B21_H16 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02542_H16 3', mRNA sequence
Length = 712
Score = 170 bits (366), Expect = 6e-042
Identities = 67/99 (67%), Positives = 78/99 (78%)
Frame = +3 / -2
Query: 9 IARHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLT 188
+ R+ Y E D VPISI WQ+PQYF+IGCAEVFTF+GQLEFFY+QAPDAMRSMCSALSLT
Sbjct: 510 MVRNNNYYELDSVPISIFWQVPQYFLIGCAEVFTFIGQLEFFYEQAPDAMRSMCSALSLT 331
Query: 189 TVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
TVALGNYLS ++G+ GWIP+NLN GH+D
Sbjct: 330 TVALGNYLSSLLVTIVTTISTKNGKAGWIPDNLNYGHID 214
Score = 101 bits (216), Expect(2) = 1e-028
Identities = 43/57 (75%), Positives = 47/57 (82%)
Frame = -1 / +2
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSS 32
PSATVVSD AEHMLL+ASGA S*KNS+CP V TSA P+ KYCG CQ +EMGT SSS
Sbjct: 317 PSATVVSDRAEHMLLIASGACS*KNSNCPMNVKTSAQPIRKYCGTCQNIEMGTESSS 487
Score = 44.1 bits (90), Expect(2) = 1e-028
Identities = 23/44 (52%), Positives = 26/44 (59%)
Frame = -1 / +2
Query: 388 FLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
FL +YQ + R A K LS SSQKK*S P + SGIHP P
Sbjct: 131 FL*VYQFEIKRKAPKFSTLSIPSSQKK*SM*P*FKLSGIHPAFP 262
>gb|DN499218.1|DN499218 T035A05.5pR Populus shoot meristem cDNA library Populus tremula x
Populus tremuloides cDNA clone T035A05 5', mRNA sequence
Length = 697
Score = 165 bits (355), Expect = 2e-040
Identities = 63/97 (64%), Positives = 76/97 (78%)
Frame = +3 / +2
Query: 15 RHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTV 194
R Y + + +P++I WQ+PQYF+ GCAEVFTF+GQLEFFYDQAPDAMRS+CSALSLTTV
Sbjct: 122 RENNYYDLEYIPMTIFWQVPQYFLTGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTTV 301
Query: 195 ALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
ALGNYL+ R G++GWIP+NLNRGHLD
Sbjct: 302 ALGNYLNTLLVTIVTKVTTRGGKLGWIPDNLNRGHLD 412
Score = 98.2 bits (208), Expect(2) = 2e-028
Identities = 39/57 (68%), Positives = 46/57 (80%)
Frame = -1 / -2
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSS 32
P+ATVV + AEH LL+ASGAWS*KNS+CP KV TSAHP+ KYCG CQ+M +G S S
Sbjct: 309 PNATVVRERAEHKLLIASGAWS*KNSNCPMKVKTSAHPVRKYCGTCQKMVIGMYSRS 139
Score = 47.3 bits (97), Expect(2) = 2e-028
Identities = 23/47 (48%), Positives = 26/47 (55%)
Frame = -1 / -2
Query: 397 SAVFLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
S FL Y + R A K R+L A SQ K*SRWP + SGI P P
Sbjct: 504 SVAFL*AYHFEIQRYAVKFRKLRIAKSQ*K*SRWPLFKLSGIQPSFP 364
>gb|DT504437.1|DT504437 WS01313.B21_F23 PTxD-IL-FL-A-4 Populus trichocarpa x Populus
deltoides cDNA clone WS01313_F23 3', mRNA sequence
Length = 821
Score = 154 bits (331), Expect = 4e-037
Identities = 62/99 (62%), Positives = 73/99 (73%)
Frame = +3 / -3
Query: 9 IARHGLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLT 188
+ R+ Y E D VPI I WQ+PQYF+IGCAEVFTF+GQLEFFY+QAPDAMRSMC ALSL
Sbjct: 627 MVRNNNYYELDSVPIFIFWQVPQYFLIGCAEVFTFIGQLEFFYEQAPDAMRSMCFALSLP 448
Query: 189 TVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
VALGNYLS ++G+ GWIP+NLN G +D
Sbjct: 447 PVALGNYLSFFLVTIVTTISTKNGKAGWIPDNLNYGPID 331
Score = 86.8 bits (183), Expect(2) = 6e-024
Identities = 38/57 (66%), Positives = 44/57 (77%)
Frame = -1 / +2
Query: 202 PSATVVSDSAEHMLLMASGAWS*KNSSCPTKVNTSAHPMMKYCGICQQMEMGTMSSS 32
PSAT SD A+HMLL+ASGA S*KNS+CP V TSA P+ KY G CQ ++MGT SSS
Sbjct: 434 PSATGGSDRAKHMLLIASGACS*KNSNCPMNVKTSAQPIRKYWGTCQNIKMGTESSS 604
Score = 43.7 bits (89), Expect(2) = 6e-024
Identities = 22/44 (50%), Positives = 26/44 (59%)
Frame = -1 / +2
Query: 388 FLYLYQLAMTR*ARKLRRLSTASSQKK*SRWPRLRFSGIHPMRP 257
FL +YQ + + A K LS SSQKK*S P + SGIHP P
Sbjct: 248 FL*VYQFEIKKKAPKFSTLSIPSSQKK*SMGP*FKLSGIHPAFP 379
>gb|CX167586.1|CX167586 F09_69-15_11.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 632
Score = 147 bits (316), Expect = 5e-035
Identities = 57/95 (60%), Positives = 70/95 (73%)
Frame = +3 / -2
Query: 21 GLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVAL 200
GL GE VPISI WQIPQ+ ++G +EVFTF+GQ+EFFY+++PDAMRS+CSALSL T +L
Sbjct: 547 GLAGEAVAVPISIIWQIPQFMLVGASEVFTFIGQIEFFYEESPDAMRSLCSALSLLTTSL 368
Query: 201 GNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGHLD 305
GNYLS G+ GWIP+NLN GHLD
Sbjct: 367 GNYLSSFILTMVAYFTTTGGKPGWIPDNLNEGHLD 263
>gb|AJ771406.1|AJ771406 AJ771406 Populus euphratica leaf 3-6 months Populus euphratica cDNA
clone P0001500006F06F1, mRNA sequence
Length = 438
Score = 131 bits (281), Expect = 3e-030
Identities = 52/90 (57%), Positives = 65/90 (72%)
Frame = +3 / +2
Query: 21 GLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVAL 200
GL GE VPISI WQIPQY ++G +EVFTF+GQ+EFFY+++PDAMRS+CSALSL T +L
Sbjct: 167 GLTGEAVAVPISIFWQIPQYMLVGASEVFTFIGQIEFFYEESPDAMRSLCSALSLLTTSL 346
Query: 201 GNYLSXXXXXXXXXXXXRHGRIGWIPENLN 290
GNYLS G+ WIP+NL+
Sbjct: 347 GNYLSSSILTMVTYFTTTGGKP*WIPDNLH 436
>gb|DN488268.1|DN488268 R045E02.3pR Populus root cDNA library Populus tremula x Populus
tremuloides cDNA clone R045E02 3', mRNA sequence
Length = 624
Score = 129 bits (276), Expect = 2e-029
Identities = 47/81 (58%), Positives = 60/81 (74%)
Frame = +3 / -3
Query: 63 WQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXXXXXX 242
WQIPQY +IG AE+FT++GQLEFFY+Q+PDAMRS+CSAL+L +LG+YLS
Sbjct: 619 WQIPQYMLIGTAEIFTYIGQLEFFYEQSPDAMRSLCSALALLAASLGSYLSSFILSMVTY 440
Query: 243 XXXRHGRIGWIPENLNRGHLD 305
G+ GW+P+NLN GHLD
Sbjct: 439 LTTTGGKTGWVPDNLNTGHLD 377
>gb|CF237321.1|CF237321 Ptajxtjxt4F7F0711 Poplar cDNA library from young tension xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 525
Score = 126 bits (270), Expect(2) = 7e-029
Identities = 50/68 (73%), Positives = 55/68 (80%)
Frame = +3 / +3
Query: 102 VFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXXXXXXXXXRHGRIGWIPE 281
VFTF+GQLEFFYDQAPDAMRS+CSALSLTTVALGNYLS R G++GWIP+
Sbjct: 3 VFTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTLLVTIVTKVTTRGGKLGWIPD 182
Query: 282 NLNRGHLD 305
NLNRGHLD
Sbjct: 183 NLNRGHLD 206
Score = 21.7 bits (41), Expect(2) = 7e-029
Identities = 6/12 (50%), Positives = 8/12 (66%)
Frame = +3 / +3
Query: 363 IASWYKYKKTAD 398
I+ WY YKK +
Sbjct: 264 ISKWYAYKKATE 299
>gb|CV243431.1|CV243431 WS02518.B21_N18 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02518_N18 3', mRNA sequence
Length = 854
Score = 115 bits (246), Expect = 2e-025
Identities = 46/85 (54%), Positives = 58/85 (68%)
Frame = +3 / -3
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
+SI WQIPQY +IG +EVF +VGQLEFF QAPD ++S SAL +T+++LGNY+S
Sbjct: 831 LSILWQIPQYVLIGASEVFMYVGQLEFFNGQAPDGLKSFGSALCMTSISLGNYVSSLLVT 652
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
R GWIP NLN+GHLD
Sbjct: 651 VVMKISTRDEMPGWIPGNLNKGHLD 577
>gb|BU886438.1|BU886438 R045E02 Populus root cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 589
Score = 111 bits (237), Expect = 4e-024
Identities = 41/74 (55%), Positives = 54/74 (72%)
Frame = +3 / +3
Query: 84 IIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXXXXXXXXXRHGR 263
+IG AE+FT++GQLEFFY+Q+PDAMRS+CSAL+L +LG+YLS G+
Sbjct: 6 LIGTAEIFTYIGQLEFFYEQSPDAMRSLCSALALLAASLGSYLSSFILSMVTYLTTTGGK 185
Query: 264 IGWIPENLNRGHLD 305
GW+P+NLN GHLD
Sbjct: 186 TGWVPDNLNTGHLD 227
>gb|CV277864.1|CV277864 WS0144.B21_M12 PTxD-IL-A-5 Populus trichocarpa x Populus deltoides
cDNA clone WS0144_M12 3', mRNA sequence
Length = 897
Score = 108 bits (230), Expect = 4e-023
Identities = 41/61 (67%), Positives = 52/61 (85%)
Frame = +3 / +3
Query: 21 GLYGEDDIVPISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVAL 200
GL GE VPISI WQIPQ+ ++G +EVFTF+GQ+EFFY+++PDAMRS+CSALSL T +L
Sbjct: 675 GLAGEAVAVPISILWQIPQFMLVGASEVFTFIGQIEFFYEESPDAMRSLCSALSLLTTSL 854
Query: 201 G 203
G
Sbjct: 855 G 857
>gb|CV266784.1|CV266784 WS0203.B21_P14 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS0203_P14 3', mRNA sequence
Length = 853
Score = 107 bits (229), Expect = 5e-023
Identities = 40/85 (47%), Positives = 58/85 (68%)
Frame = +3 / +1
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
+SI WQIPQY ++G +EVF +VGQLEFF QAPD ++S S+L + +++LGNY+S
Sbjct: 496 LSIFWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGIKSFGSSLCMASISLGNYVSSMLVS 675
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
+ + GWIP++LN GH+D
Sbjct: 676 MVMKITAKGDKPGWIPDDLNTGHMD 750
>gb|CK318837.1|CK318837 X9P03h07 Populus stem seasonal library Populus deltoides cDNA, mRNA
sequence
Length = 592
Score = 105 bits (225), Expect = 2e-022
Identities = 39/85 (45%), Positives = 57/85 (67%)
Frame = +3 / +1
Query: 51 ISICWQIPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXX 230
+SI WQIPQY ++G +EVF ++GQLEFF QAPD ++S S+L + +++LGNY S
Sbjct: 91 LSIFWQIPQYVLVGASEVFMYIGQLEFFNGQAPDGIKSFGSSLCMASISLGNYASSMLVN 270
Query: 231 XXXXXXXRHGRIGWIPENLNRGHLD 305
+ + GWIP++LN GH+D
Sbjct: 271 MVMKITTKGDKPGWIPDDLNTGHMD 345
>gb|DN497951.1|DN497951 R045E02.5pR Populus root cDNA library Populus tremula x Populus
tremuloides cDNA clone R045E02 5', mRNA sequence
Length = 849
Score = 87.2 bits (184), Expect(2) = 2e-021
Identities = 33/62 (53%), Positives = 42/62 (67%)
Frame = +3 / +2
Query: 120 QLEFFYDQAPDAMRSMCSALSLTTVALGNYLSXXXXXXXXXXXXRHGRIGWIPENLNRGH 299
QLEFFY+Q+P AMRS+CSAL+L +LG+YLS G+ GW+P+NLN GH
Sbjct: 65 QLEFFYEQSPHAMRSLCSALALLAASLGSYLSSFILSMVTYLTTTGGKTGWVPDNLNTGH 244
Query: 300 LD 305
LD
Sbjct: 245 LD 250
Score = 35.0 bits (70), Expect(2) = 2e-021
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = +3 / +3
Query: 63 WQIPQYFIIGCAEVFT 110
WQIPQY +IG A++FT
Sbjct: 6 WQIPQYMLIGTADIFT 53
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 249,086,095
Number of Sequences: 369679
Number of extensions: 3042088
Number of successful extensions: 42348
Number of sequences better than 1.0e-020: 16
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7342388850
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)