TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204398.2.1
(666 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX169214.1|CX169214 H01_69-102_15.ab1 leaf inoculated wi... 170 1e-041
gb|DN485453.1|DN485453 N005G05.3pR Populus bark cDNA librar... 167 5e-041
gb|CV274273.1|CV274273 WS0172.B21_M01 PTxD-NR-A-8 Populus t... 155 3e-037
gb|CK088245.1|CK088245 A044P79.3pR Hybrid aspen plasmid lib... 96 3e-021
>gb|CX169214.1|CX169214 H01_69-102_15.ab1 leaf inoculated with Marssonia pathogen of
Populus deltoides Populus deltoides cDNA, mRNA sequence
Length = 636
Score = 170 bits (365), Expect = 1e-041
Identities = 70/94 (74%), Positives = 82/94 (87%)
Frame = +1 / -2
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSG+FE AL IL+C+ENPAKYF KLLRKAMKGLGT+D LIRV+VTRTEIDMQYIKAEY
Sbjct: 515 TSGHFEHALKTILQCSENPAKYFVKLLRKAMKGLGTNDTALIRVIVTRTEIDMQYIKAEY 336
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVGPGH*V 282
KKY+K L +A+HSETSG+YR FLL+L+GP *+
Sbjct: 335 LKKYRKTLNDAVHSETSGHYRAFLLALLGPNQ*L 234
Score = 116 bits (249), Expect = 1e-025
Identities = 53/89 (59%), Positives = 61/89 (68%)
Frame = -2 / +1
Query: 269 GPTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFM 90
GP RR * P+VSE AS N FLYF +YSA IYC+SISVLVT TLI +S VPRPF+
Sbjct: 247 GPKRARRKAL**PEVSE*TASFNVFLYFFRYSAFIYCMSISVLVTITLIRAVSLVPRPFI 426
Query: 89 AFRKSFAKYFAGFSAHLRIAKSANSKFPD 3
A R + KYFAGFS H +I A SK P+
Sbjct: 427 ALRSNLTKYFAGFSEHCKIVFKACSKCPE 513
>gb|DN485453.1|DN485453 N005G05.3pR Populus bark cDNA library Populus tremula x Populus
tremuloides cDNA clone N005G05 3', mRNA sequence
Length = 848
Score = 167 bits (360), Expect = 5e-041
Identities = 69/94 (73%), Positives = 81/94 (86%)
Frame = +1 / -1
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSG+FE AL IL+C+ENPA YF KLLRKAMKGLGT+D LIRV+VTRTEIDMQYIKAEY
Sbjct: 545 TSGHFEHALKTILQCSENPAMYFVKLLRKAMKGLGTNDSALIRVIVTRTEIDMQYIKAEY 366
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVGPGH*V 282
KKY+K L +A+HSETSG+YR FLL+L+GP *+
Sbjct: 365 LKKYRKTLNDAVHSETSGHYRAFLLALLGPNQ*L 264
Score = 116 bits (249), Expect = 1e-025
Identities = 54/89 (60%), Positives = 60/89 (67%)
Frame = -2 / +1
Query: 269 GPTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFM 90
GP RR * P+VSE AS N FLYF +YSA IYC+SISVLVT TLI LS VPRPF+
Sbjct: 277 GPKRARRKAL**PEVSE*TASFNVFLYFFRYSAFIYCMSISVLVTITLIRALSLVPRPFI 456
Query: 89 AFRKSFAKYFAGFSAHLRIAKSANSKFPD 3
A R S KY AGFS H +I A SK P+
Sbjct: 457 ALRSSLTKYIAGFSEHCKIVFKACSKCPE 543
>gb|CV274273.1|CV274273 WS0172.B21_M01 PTxD-NR-A-8 Populus trichocarpa x Populus deltoides
cDNA clone WS0172_M01 3', mRNA sequence
Length = 835
Score = 155 bits (333), Expect = 3e-037
Identities = 63/90 (70%), Positives = 75/90 (83%)
Frame = +1 / -3
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSG+FE AL IL C+ENPA YFAK+L KAMKG+GT+D LIRV+VTRTEIDM YIKAEY
Sbjct: 509 TSGHFEHALKTILLCSENPANYFAKVLPKAMKGMGTNDPALIRVIVTRTEIDMHYIKAEY 330
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVGP 270
KKYKK L +A+HSETSG+Y+ FL + +GP
Sbjct: 329 LKKYKKTLNDAVHSETSGHYQAFLFAFLGP 240
Score = 105 bits (225), Expect = 2e-022
Identities = 52/89 (58%), Positives = 59/89 (66%)
Frame = -2 / +1
Query: 269 GPTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFM 90
GP +R *+P+VSE AS N FLYF KYSA I C+SISVLVT TLIS S VP PF+
Sbjct: 241 GPKKAKRKA**WPEVSE*TASFNVFLYFFKYSAFI*CMSISVLVTITLISAGSLVPMPFI 420
Query: 89 AFRKSFAKYFAGFSAHLRIAKSANSKFPD 3
A + AK FAGFS H +I A SK PD
Sbjct: 421 ALGSTLAK*FAGFSEHSKIVFKACSKCPD 507
>gb|CK088245.1|CK088245 A044P79.3pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A044P79 3', mRNA sequence
Length = 527
Score = 95.9 bits (203), Expect(2) = 3e-021
Identities = 41/83 (49%), Positives = 55/83 (66%)
Frame = +1 / -2
Query: 25 LLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEYFKKYKKPL 204
L A ++C P KYF KLLR ++K LGTD++ LIRVV TR E+DM+ IK EY ++ L
Sbjct: 472 LKATIKCLTYPEKYFEKLLRLSIKKLGTDERALIRVVTTRAEVDMERIKEEYHRRNSVTL 293
Query: 205 AEAIHSETSGNYRTFLLSLVGPG 273
I +TSG+Y LL+L+G G
Sbjct: 292 DRDIAGDTSGDYERMLLALIGHG 224
Score = 25.4 bits (49), Expect(2) = 3e-021
Identities = 14/53 (26%), Positives = 23/53 (43%)
Frame = +1 / -1
Query: 292 PWDMIFHPSTLNAATYLENL*CCHLSVVTRGFV*LPHMRYLVCLTKVSVFKFV 450
PW + + S + + L CC ++ + + R L CL VS+ FV
Sbjct: 230 PWRCLIYFSWMQKSCALYECCCCCIAFARHNLPDVSYHRCLYCLY*VSLKCFV 72
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 329,330,354
Number of Sequences: 369679
Number of extensions: 4613412
Number of successful extensions: 42640
Number of sequences better than 1.0e-020: 4
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8321374030
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)