TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115474.2.1
(798 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU887462.1|BU887462 R061D12 Populus root cDNA library Po... 145 7e-054
gb|CX183006.1|CX183006 F11_45-25_11.ab1 leaf inoculated wit... 139 5e-050
gb|BI122552.1|BI122552 I008P96P Populus leaf cDNA library P... 73 2e-024
gb|CK103987.1|CK103987 I008P96.5pR Populus senescing leaves... 74 2e-023
>gb|BU887462.1|BU887462 R061D12 Populus root cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 503
Score = 145 bits (312), Expect(3) = 7e-054
Identities = 63/94 (67%), Positives = 75/94 (79%)
Frame = -3 / +2
Query: 460 GIDKLHCRNGVVGNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAI 281
G D + RNG + +L LL +++++VDDN VNL VAA ALKKYGA+V CADSG++AI
Sbjct: 47 GGDMGNPRNGEHPSLSLRKLLVGRKMLIVDDNKVNLMVAAAALKKYGADVVCADSGQKAI 226
Query: 280 TLLKPPHNFDACFMDIQMPEMDGFEATKRIRVME 179
LLKPPH FDACFMDIQMPEMDGFEAT+RIR ME
Sbjct: 227 KLLKPPHKFDACFMDIQMPEMDGFEATRRIRDME 328
Score = 111 bits (238), Expect(3) = 2e-035
Identities = 56/94 (59%), Positives = 67/94 (71%)
Frame = +3 / -3
Query: 180 SITLILFVASNPSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRF 359
SI+ IL VASNPSISGI +SMK AS L*GG S++IA +PLSAQ TSAPYFF+A AAT+RF
Sbjct: 327 SISRILLVASNPSISGIWISMKHASNL*GGLSNLIAFWPLSAQTTSAPYFFKAAAATMRF 148
Query: 360 TILSSTTMIRFL*RRLPSVELPTTPFLQCSLSIP 461
T+LSST I R L +L +P +S P
Sbjct: 147 TLLSSTISIFLPTRSLRRDKLGCSPLRGFPMSPP 46
Score = 72.5 bits (152), Expect(3) = 7e-054
Identities = 29/38 (76%), Positives = 31/38 (81%)
Frame = -3 / +2
Query: 115 WRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
W PILAMTADVIQAT E+C + MDGYVSKPFE EQL
Sbjct: 335 WHIPILAMTADVIQATSEECQRCGMDGYVSKPFEAEQL 448
Score = 56.1 bits (116), Expect(3) = 2e-035
Identities = 24/36 (66%), Positives = 28/36 (77%)
Frame = +3 / -3
Query: 3 NCSPSNGLET*PSISDFKHCSCVAWITSAVMANIGV 110
+CS SNGLET PSI H S VAWITSAV+A+IG+
Sbjct: 447 SCSASNGLETYPSIPHLWHSSDVAWITSAVIASIGI 340
Score = 31.3 bits (62), Expect(3) = 7e-054
Identities = 12/17 (70%), Positives = 15/17 (88%)
Frame = -3 / +2
Query: 508 PLRASMLQVSLQRALGG 458
PL+AS+L SLQRA+GG
Sbjct: 2 PLKASLLAASLQRAMGG 52
Score = 21.7 bits (41), Expect(3) = 2e-035
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = +2 / -2
Query: 458 PTQCSL*RNLKHAC 499
PT C+L*R+ + C
Sbjct: 52 PTHCTL*RSSQQTC 11
>gb|CX183006.1|CX183006 F11_45-25_11.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 620
Score = 139 bits (299), Expect(3) = 5e-050
Identities = 59/86 (68%), Positives = 69/86 (80%)
Frame = -3 / +1
Query: 436 NGVVGNSTLGSLLHKKRIIVVDDNIVNLKVAAGALKKYGAEVTCADSGKEAITLLKPPHN 257
NG + +L LL +++++VDDN VNL VAA ALKKYGA+V CADSG+ AI LLKPPH
Sbjct: 124 NGEHPSLSLRKLLVGRKMLIVDDNKVNLMVAAAALKKYGADVVCADSGQRAIKLLKPPHK 303
Query: 256 FDACFMDIQMPEMDGFEATKRIRVME 179
FDACFMDIQ PEMDGFEAT+RIR ME
Sbjct: 304 FDACFMDIQTPEMDGFEATRRIRDME 381
Score = 110 bits (234), Expect(3) = 1e-037
Identities = 53/85 (62%), Positives = 64/85 (75%)
Frame = +3 / -1
Query: 180 SITLILFVASNPSISGICMSMKQASKL*GGFSSVIASFPLSAQVTSAPYFFRAPAATLRF 359
SI+ IL VASNPSISG+ +SMK AS L*GG S++IA +PLSAQ TSAPYFF+A AAT+RF
Sbjct: 380 SISRILLVASNPSISGVWISMKHASNL*GGLSNLIALWPLSAQTTSAPYFFKAAAATMRF 201
Query: 360 TILSSTTMIRFL*RRLPSVELPTTP 434
T+LSST I R L +L +P
Sbjct: 200 TLLSSTISIFLPTRSLRRDKLGCSP 126
Score = 73.9 bits (155), Expect(3) = 5e-050
Identities = 29/38 (76%), Positives = 32/38 (84%)
Frame = -3 / +1
Query: 115 WRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
W PILAMTADVIQAT+E+C + MDGYVSKPFE EQL
Sbjct: 388 WHIPILAMTADVIQATYEECQRCGMDGYVSKPFEAEQL 501
Score = 56.5 bits (117), Expect(3) = 1e-037
Identities = 24/36 (66%), Positives = 28/36 (77%)
Frame = +3 / -1
Query: 3 NCSPSNGLET*PSISDFKHCSCVAWITSAVMANIGV 110
+CS SNGLET PSI H S VAWITSAV+A+IG+
Sbjct: 500 SCSASNGLETYPSIPHLWHSSYVAWITSAVIASIGI 393
Score = 51.5 bits (106), Expect(3) = 1e-022
Identities = 21/50 (42%), Positives = 30/50 (60%)
Frame = -2 / +3
Query: 350 GCCRRSKEIWRGSNLC*QWEGSNHTAETSSQF*CLFHGHTDA*NGWI*SH 201
G C +E+W LC W + A +SQ *C+ HG+ +A*NGW+*S+
Sbjct: 210 GSCCCFEEVWS*CCLCR*WPKGDQIA*ATSQI*CMLHGYPNA*NGWV*SY 359
Score = 46.9 bits (96), Expect(3) = 1e-022
Identities = 21/38 (55%), Positives = 26/38 (68%)
Frame = -1 / +2
Query: 117 SGELQY*P*QQMLSRRHMNNA*NLKWMVMFPSHSRASN 4
+G QY * QM SR HM NA ++ WM MFPSHS+ +N
Sbjct: 386 TGIYQYWQ*LQM*SRLHMRNAKDVGWMDMFPSHSKLNN 499
Score = 45.5 bits (93), Expect(3) = 1e-022
Identities = 26/65 (40%), Positives = 35/65 (53%)
Frame = -3 / +1
Query: 523 NVIMKPLRASMLQVSLQRALGGIDKLHCRNGVVGNSTLGSLLHKKRIIVVDDNIVNLKVA 344
+VI KPL+ASML SLQRA+GG C S L+ +K +IV D+ + + A
Sbjct: 40 SVITKPLKASMLAASLQRAMGGNKGNPCNGEHPSLSLRKLLVGRKMLIVDDNKVNLMVAA 219
Query: 343 AGALK 329
A K
Sbjct: 220 AALKK 234
Score = 28.1 bits (55), Expect(3) = 1e-037
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +2 / -3
Query: 452 IYPTQCSL*RNLKHACTKWL 511
+ PT CSL*R+ +H C + L
Sbjct: 111 LIPTHCSL*RSSQHTCLEGL 52
Score = 23.1 bits (44), Expect(3) = 5e-050
Identities = 7/14 (50%), Positives = 8/14 (57%)
Frame = -1 / +2
Query: 498 QACFRFRYKEHWVG 457
Q C+ Y E WVG
Sbjct: 65 QVCWLLLYNEQWVG 106
>gb|BI122552.1|BI122552 I008P96P Populus leaf cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 456
Score = 72.5 bits (152), Expect(3) = 2e-024
Identities = 29/38 (76%), Positives = 31/38 (81%)
Frame = -3 / +2
Query: 115 WRTPILAMTADVIQATHEQCLKSEMDGYVSKPFEGEQL 2
W PILAMTADVIQAT E+C + MDGYVSKPFE EQL
Sbjct: 107 WHIPILAMTADVIQATSEECQRCGMDGYVSKPFEAEQL 220
Score = 42.3 bits (86), Expect(3) = 2e-024
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -3 / +2
Query: 235 IQMPEMDGFEATKRIRVME 179
IQMPEMDGFEAT+RIR ME
Sbjct: 44 IQMPEMDGFEATRRIRDME 100
Score = 35.4 bits (71), Expect(3) = 2e-024
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = -3 / +1
Query: 277 LLKPPHNFDACFM 239
LLKPPH FDACFM
Sbjct: 1 LLKPPHKFDACFM 39
>gb|CK103987.1|CK103987 I008P96.5pR Populus senescing leaves cDNA library Populus tremula
cDNA clone I008P96 5', mRNA sequence
Length = 624
Score = 74.4 bits (156), Expect(3) = 2e-023
Identities = 28/32 (87%), Positives = 30/32 (93%)
Frame = -3 / +1
Query: 274 LKPPHNFDACFMDIQMPEMDGFEATKRIRVME 179
+KPPH FDACFMDIQMPEMDGFEAT+RIR ME
Sbjct: 37 VKPPHKFDACFMDIQMPEMDGFEATRRIRDME 132
Score = 53.8 bits (111), Expect(3) = 2e-023
Identities = 21/29 (72%), Positives = 23/29 (79%)
Frame = -3 / +1
Query: 115 WRTPILAMTADVIQATHEQCLKSEMDGYV 29
W PILAMTADVIQAT E+C + MDGYV
Sbjct: 139 WHIPILAMTADVIQATSEECQRCGMDGYV 225
Score = 21.7 bits (41), Expect(3) = 2e-023
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3 / +3
Query: 28 SKPFEGEQL 2
SKPFE EQL
Sbjct: 225 SKPFEAEQL 251
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 447,824,749
Number of Sequences: 369679
Number of extensions: 6960936
Number of successful extensions: 45329
Number of sequences better than 1.0e-020: 5
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 10347450540
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)