TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.2
(682 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT512918.1|DT512918 WS02420.B21_J19 PTxD-ICC-N-A-14 Popu... 80 1e-024
gb|CV253324.1|CV253324 WS0221.B21_J07 PTxD-ICC-A-12 Populus... 76 2e-023
gb|CV243754.1|CV243754 WS0252.B21_M05 PT-MB-N-A-15 Populus ... 80 2e-022
gb|DN486268.1|DN486268 P012D09.3pR Populus petioles cDNA li... 73 4e-022
gb|CK318807.1|CK318807 X9P03f01 Populus stem seasonal libra... 73 4e-022
gb|BU888780.1|BU888780 P012D09 Populus petioles cDNA librar... 73 4e-022
>gb|DT512918.1|DT512918 WS02420.B21_J19 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02420_J19 3', mRNA sequence
Length = 891
Score = 80.3 bits (169), Expect(3) = 1e-024
Identities = 31/81 (38%), Positives = 47/81 (58%)
Frame = +1 / -1
Query: 139 ASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITL 318
A L++ M NV PI+IDQ YCP S V ++DV +++IHG+S T A+
Sbjct: 417 ARNILFQHAVMTNVQNPIVIDQNYCPGEKGCPGQVSGVKISDVTYQDIHGSSATELAVKF 238
Query: 319 NCANNLPCQGVQLVNVDIKYN 381
+C+ PC G++L +V + YN
Sbjct: 237 DCSKTNPCSGIKLEDVKLTYN 175
Score = 46.0 bits (94), Expect(3) = 1e-024
Identities = 19/38 (50%), Positives = 26/38 (68%)
Frame = +1 / -1
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSY 114
GIS+GSLG+ E V++V V T GT NG+RIK++
Sbjct: 552 GISIGSLGKDSQEAGVQNVTVKTTTFTGTENGVRIKTW 439
Score = 24.4 bits (47), Expect(3) = 1e-024
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +1 / -1
Query: 367 DIKYNGSGNKTMAVCKNAIGKSIGLAKELACI* 465
D+K + A C NA G + G+ + +C+*
Sbjct: 195 DVKLTYNNQPAEASCSNAGGVASGVVQPTSCL* 97
>gb|CV253324.1|CV253324 WS0221.B21_J07 PTxD-ICC-A-12 Populus trichocarpa x Populus
deltoides cDNA clone WS0221_J07 3', mRNA sequence
Length = 722
Score = 76.2 bits (160), Expect(3) = 2e-023
Identities = 30/81 (37%), Positives = 46/81 (56%)
Frame = +1 / -2
Query: 139 ASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITL 318
A L++ M NV PI+IDQ YCP S V ++DV +++IHG+S T A+
Sbjct: 571 ARNILFQHAVMTNVQNPIVIDQNYCPGEKGCPGQVSGVKISDVTYQDIHGSSATELAVKF 392
Query: 319 NCANNLPCQGVQLVNVDIKYN 381
+C+ PC G++L +V + N
Sbjct: 391 DCSKTNPCSGIKLEDVKLTCN 329
Score = 46.0 bits (94), Expect(3) = 2e-023
Identities = 19/38 (50%), Positives = 26/38 (68%)
Frame = +1 / -2
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSY 114
GIS+GSLG+ E V++V V T GT NG+RIK++
Sbjct: 706 GISIGSLGKDSQEAGVQNVTVKTTTFTGTENGVRIKTW 593
Score = 24.4 bits (47), Expect(3) = 2e-023
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +1 / -2
Query: 367 DIKYNGSGNKTMAVCKNAIGKSIGLAKELACI* 465
D+K + A C NA G + G+ + +C+*
Sbjct: 349 DVKLTCNNQPAEASCSNAGGVASGVVQPTSCL* 251
>gb|CV243754.1|CV243754 WS0252.B21_M05 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS0252_M05 3', mRNA sequence
Length = 928
Score = 79.9 bits (168), Expect(2) = 2e-022
Identities = 34/84 (40%), Positives = 52/84 (61%)
Frame = +1 / -3
Query: 139 ASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITL 318
A+ ++ I M+NVS PIIIDQ YC ++ + S V V ++ F+ I GTS T EAI
Sbjct: 716 ATNITFQNIFMENVSNPIIIDQYYCDAHVPCANQTSAVKVENISFRRIKGTSATEEAIKF 537
Query: 319 NCANNLPCQGVQLVNVDIKYNGSG 390
C+++LPC+G+ L +V + + G
Sbjct: 536 ACSDDLPCKGLYLEDVQLLSHTGG 465
Score = 45.5 bits (93), Expect(2) = 2e-022
Identities = 18/39 (46%), Positives = 26/39 (66%)
Frame = +1 / -3
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYE 117
GIS+GSLG+ V DV V G ++ T NG+RIK+++
Sbjct: 848 GISIGSLGKSNSSSLVRDVMVDGAFLSNTDNGVRIKTWQ 732
>gb|DN486268.1|DN486268 P012D09.3pR Populus petioles cDNA library Populus tremula cDNA
clone P012D09 3', mRNA sequence
Length = 768
Score = 73.0 bits (153), Expect(2) = 4e-022
Identities = 30/81 (37%), Positives = 49/81 (60%)
Frame = +1 / -2
Query: 142 SKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLN 321
S ++ I M+ V+ PIIIDQ YC + + S + V +VV+KNI GTS + A+ +
Sbjct: 527 SNIKFQNIEMNKVANPIIIDQNYCDQDKPCRQQKSALQVKNVVYKNIKGTSASEVAMRFD 348
Query: 322 CANNLPCQGVQLVNVDIKYNG 384
C+ PCQG+ L +++++ G
Sbjct: 347 CSKAYPCQGILLQDINLERAG 285
Score = 51.5 bits (106), Expect(2) = 4e-022
Identities = 20/50 (40%), Positives = 32/50 (64%)
Frame = +1 / -2
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKF 150
GIS+GSLG + + V +V V G ++GT NG+RIK+++ ++ KF
Sbjct: 662 GISIGSLGVHDSKDHVSEVTVNGAKLSGTANGVRIKTWQGGSGNVSNIKF 513
>gb|CK318807.1|CK318807 X9P03f01 Populus stem seasonal library Populus deltoides cDNA, mRNA
sequence
Length = 736
Score = 73.0 bits (153), Expect(2) = 4e-022
Identities = 30/81 (37%), Positives = 49/81 (60%)
Frame = +1 / +2
Query: 142 SKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLN 321
S ++ I M+ V+ PIIIDQ YC + K S + V +V++KNI GTS + A+ +
Sbjct: 263 SNIKFQNIEMNKVTNPIIIDQNYCDQDKPCKQQKSALQVKNVMYKNIKGTSASEVAMRFD 442
Query: 322 CANNLPCQGVQLVNVDIKYNG 384
C+ PCQG+ L +++++ G
Sbjct: 443 CSKAYPCQGILLQDINLERAG 505
Score = 51.5 bits (106), Expect(2) = 4e-022
Identities = 20/50 (40%), Positives = 32/50 (64%)
Frame = +1 / +2
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKF 150
GIS+GSLG + + V V V+G ++GT NG+RIK+++ ++ KF
Sbjct: 128 GISIGSLGAHDSKDHVSGVTVSGAKLSGTANGVRIKTWQGGSGNVSNIKF 277
>gb|BU888780.1|BU888780 P012D09 Populus petioles cDNA library Populus tremula cDNA 5 prime,
mRNA sequence
Length = 728
Score = 73.0 bits (153), Expect(2) = 4e-022
Identities = 30/81 (37%), Positives = 49/81 (60%)
Frame = +1 / +3
Query: 142 SKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLN 321
S ++ I M+ V+ PIIIDQ YC + + S + V +VV+KNI GTS + A+ +
Sbjct: 378 SNIKFQNIEMNKVANPIIIDQNYCDQDKPCRQQKSALQVKNVVYKNIKGTSASEVAMRFD 557
Query: 322 CANNLPCQGVQLVNVDIKYNG 384
C+ PCQG+ L +++++ G
Sbjct: 558 CSKAYPCQGILLQDINLERAG 620
Score = 51.5 bits (106), Expect(2) = 4e-022
Identities = 20/50 (40%), Positives = 32/50 (64%)
Frame = +1 / +3
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKF 150
GIS+GSLG + + V +V V G ++GT NG+RIK+++ ++ KF
Sbjct: 243 GISIGSLGVHDSKDHVSEVTVNGAKLSGTANGVRIKTWQGGSGNVSNIKF 392
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 281,933,773
Number of Sequences: 369679
Number of extensions: 3789288
Number of successful extensions: 40588
Number of sequences better than 1.0e-020: 6
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8566120325
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)