TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067043.2.1
(834 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV274873.1|CV274873 WS0174.B21.1_K13 PTxD-NR-A-8 Populus... 216 2e-055
gb|DT520673.1|DT520673 WS02448.B21_D13 PTxD-ICC-N-A-14 Popu... 216 2e-055
gb|CN523137.1|CN523137 GQ015M01.B3_L24 GQ015 Populus tricho... 212 3e-054
gb|CV257819.1|CV257819 WS0247.B21_L22 PTxD-ICC-N-A-14 Popul... 169 1e-053
gb|CN520070.1|CN520070 GQ0107.B3_A09 GQ010 Populus trichoca... 167 9e-041
gb|CN549936.1|CN549936 GQ0243.B3_L22 GQ024 Populus trichoca... 137 8e-032
gb|BU836172.1|BU836172 T083E02 Populus apical shoot cDNA li... 128 5e-029
gb|DN492268.1|DN492268 V059F02.3pR Populus male catkins cDN... 122 4e-027
gb|CN523227.1|CN523227 GQ015M01.B3_J05 GQ015 Populus tricho... 113 2e-024
gb|BU879394.1|BU879394 V059F02 Populus flower cDNA library ... 111 4e-024
>gb|CV274873.1|CV274873 WS0174.B21.1_K13 PTxD-NR-A-8 Populus trichocarpa x Populus
deltoides cDNA clone WS0174_K13 3', mRNA sequence
Length = 885
Score = 216 bits (466), Expect = 2e-055
Identities = 84/197 (42%), Positives = 121/197 (61%)
Frame = +3 / -1
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E E L+A+ Y+ P++ PFFP DLP P+ P P+VLHP A + +N +W MYF EL
Sbjct: 882 ETEKLHAQLGRYKSPDEHPFFPDDLPEPVLPPLQYPQVLHPIADSININKTVWDMYFREL 703
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNS 362
+P +GDDG+ T D CLQ LS+RIH G++VAE KF+ +P +Y I+ +DS
Sbjct: 702 IPRLVKEGDDGNCGSTAVCDTICLQALSKRIHYGEFVAEAKFRASPDDYEAAIRAQDSKR 523
Query: 363 LMDMLTFXXXXXXXXXXXXXXXXTFGQNVTLDDNATAGDSECKVDPKVLSKLYDQWVMPL 542
LM++LT+ FGQ VT++ D K+ P +++ LY +W+MPL
Sbjct: 522 LMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGEIDGTDPVYKIRPTLVADLYGEWIMPL 343
Query: 543 TKDVEVEYLLRRLD*SV 593
TK+V+V+YLLRRLD*++
Sbjct: 342 TKEVQVQYLLRRLD*AL 292
Score = 104 bits (221), Expect = 1e-021
Identities = 62/167 (37%), Positives = 75/167 (44%)
Frame = -3 / +2
Query: 544 VSGITH*SYSLESTLGSTLHSLSPAVALSSKVTFCPNVXXXXXXXXXXXXXXXLNVSISI 365
VSG+ H Y + +G L++ S S VT CP V ISI
Sbjct: 341 VSGMIHSPYKSATRVGRILYTGSVPSISPSIVTSCPKALAFISTLFFTASSTAGYVRISI 520
Query: 364 RELLSFSFISRLYS*GASLNFTSATYLPI*ILCDRTCRHAKSSATVCA*LPSSPSTVNNG 185
LLS + ++ S G + N SAT P IL +R CR S V LPSSPS N G
Sbjct: 521 SLLLSCALMAAS*SSGEARNLASATNSP*WILFERACRQIVSQTAVDPQLPSSPSLTNLG 700
Query: 184 SNSSKYIFHIASFTVTNEAKGCKTFGEGFVGKRGEGRS*GKNGTSSG 44
+S KY+ H F A GC T G G G G+S GK G SSG
Sbjct: 701 ISSLKYMSHTVLFIFIESAMGCSTCGYCKGGNTGSGKSSGKKGCSSG 841
>gb|DT520673.1|DT520673 WS02448.B21_D13 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02448_D13 3', mRNA sequence
Length = 897
Score = 216 bits (466), Expect = 2e-055
Identities = 84/197 (42%), Positives = 121/197 (61%)
Frame = +3 / -1
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E E L+A+ Y+ P++ PFFP DLP P+ P P+VLHP A + +N +W MYF EL
Sbjct: 801 ETEKLHAQLGRYKSPDEHPFFPDDLPEPVLPPLQYPQVLHPIADSININKTVWDMYFREL 622
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNS 362
+P +GDDG+ T D CLQ LS+RIH G++VAE KF+ +P +Y I+ +DS
Sbjct: 621 IPRLVKEGDDGNCGSTAVCDTICLQALSKRIHYGEFVAEAKFRASPDDYEAAIRAQDSKR 442
Query: 363 LMDMLTFXXXXXXXXXXXXXXXXTFGQNVTLDDNATAGDSECKVDPKVLSKLYDQWVMPL 542
LM++LT+ FGQ VT++ D K+ P +++ LY +W+MPL
Sbjct: 441 LMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGEIDGTDPVYKIRPTLVADLYGEWIMPL 262
Query: 543 TKDVEVEYLLRRLD*SV 593
TK+V+V+YLLRRLD*++
Sbjct: 261 TKEVQVQYLLRRLD*AL 211
Score = 104 bits (221), Expect = 1e-021
Identities = 62/167 (37%), Positives = 75/167 (44%)
Frame = -3 / +2
Query: 544 VSGITH*SYSLESTLGSTLHSLSPAVALSSKVTFCPNVXXXXXXXXXXXXXXXLNVSISI 365
VSG+ H Y + +G L++ S S VT CP V ISI
Sbjct: 260 VSGMIHSPYKSATRVGRILYTGSVPSISPSIVTSCPKALAFISTLFFTASSTAGYVRISI 439
Query: 364 RELLSFSFISRLYS*GASLNFTSATYLPI*ILCDRTCRHAKSSATVCA*LPSSPSTVNNG 185
LLS + ++ S G + N SAT P IL +R CR S V LPSSPS N G
Sbjct: 440 SLLLSCALMAAS*SSGEARNLASATNSP*WILFERACRQIVSQTAVDPQLPSSPSLTNLG 619
Query: 184 SNSSKYIFHIASFTVTNEAKGCKTFGEGFVGKRGEGRS*GKNGTSSG 44
+S KY+ H F A GC T G G G G+S GK G SSG
Sbjct: 620 ISSLKYMSHTVLFIFIESAMGCSTCGYCKGGNTGSGKSSGKKGCSSG 760
>gb|CN523137.1|CN523137 GQ015M01.B3_L24 GQ015 Populus trichocarpa x Populus deltoides cDNA
clone GQ015M01_L24 5', mRNA sequence
Length = 785
Score = 212 bits (457), Expect = 3e-054
Identities = 82/197 (41%), Positives = 119/197 (60%)
Frame = +3 / +1
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E E L+A+ Y+ P++ PFFP DLP P+ P P+VLHP + +N +W MYF EL
Sbjct: 88 ETEKLHAQLGRYKSPDEHPFFPDDLPEPVLPPLQYPQVLHPITDSININKTVWDMYFREL 267
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNS 362
+P +GDDG+ T D CLQ LS+RIH G++VAE KF+ +P +Y I+ +DS
Sbjct: 268 IPRLVKEGDDGNCGSTAVCDTICLQALSKRIHYGEFVAEAKFRASPDDYEAAIRAQDSKR 447
Query: 363 LMDMLTFXXXXXXXXXXXXXXXXTFGQNVTLDDNATAGDSECKVDPKVLSKLYDQWVMPL 542
LM++LT+ FGQ VT++ D K+ P +++ LY +W+MPL
Sbjct: 448 LMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGEIDGTDPVYKIRPTLVADLYGEWIMPL 627
Query: 543 TKDVEVEYLLRRLD*SV 593
TK+V+V+YLLRR D*++
Sbjct: 628 TKEVQVQYLLRRXD*AL 678
Score = 102 bits (217), Expect = 3e-021
Identities = 61/167 (36%), Positives = 74/167 (44%)
Frame = -3 / -1
Query: 544 VSGITH*SYSLESTLGSTLHSLSPAVALSSKVTFCPNVXXXXXXXXXXXXXXXLNVSISI 365
VSG+ H Y + +G L++ S S VT CP V ISI
Sbjct: 629 VSGMIHSPYKSATRVGRILYTGSVPSISPSIVTSCPKALAFISTLFFTASSTAGYVRISI 450
Query: 364 RELLSFSFISRLYS*GASLNFTSATYLPI*ILCDRTCRHAKSSATVCA*LPSSPSTVNNG 185
LLS + ++ S G + N SAT P IL +R CR S V LPSSPS N G
Sbjct: 449 SLLLSCALMAAS*SSGEARNLASATNSP*WILFERACRQIVSQTAVDPQLPSSPSLTNLG 270
Query: 184 SNSSKYIFHIASFTVTNEAKGCKTFGEGFVGKRGEGRS*GKNGTSSG 44
+S KY+ H F GC T G G G G+S GK G SSG
Sbjct: 269 ISSLKYMSHTVLFIFIESVMGCSTCGYCKGGNTGSGKSSGKKGCSSG 129
>gb|CV257819.1|CV257819 WS0247.B21_L22 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0247_L22 3', mRNA sequence
Length = 752
Score = 169 bits (363), Expect(2) = 1e-053
Identities = 68/124 (54%), Positives = 88/124 (70%)
Frame = +3 / -1
Query: 9 EALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDELLP 188
EA+ AKA Y PE+ PFFP +LP L P +VLH + + +N AIW MY ++LLP
Sbjct: 749 EAVQAKAGRYVNPEENPFFPDNLPPSLVPNYNYSQVLHSGGASININKAIWDMYLNQLLP 570
Query: 189 LFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLM 368
LF V GDDG+YA T A DL+CLQ LS+RIH GK+VAE+KF+DAPQ+Y I+ KD++ LM
Sbjct: 569 LFVVAGDDGNYASTAASDLSCLQALSRRIHYGKFVAEIKFRDAPQDYEPPIRAKDADELM 390
Query: 369 DMLT 380
+LT
Sbjct: 389 KLLT 378
Score = 61.6 bits (128), Expect(2) = 1e-053
Identities = 24/33 (72%), Positives = 30/33 (90%)
Frame = +3 / -1
Query: 489 KVDPKVLSKLYDQWVMPLTKDVEVEYLLRRLD* 587
KVDP V+++LYD+WV+ LTK VEVEYL+RRLD*
Sbjct: 275 KVDPSVVARLYDEWVISLTKRVEVEYLIRRLD* 177
>gb|CN520070.1|CN520070 GQ0107.B3_A09 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0107_A09 5', mRNA sequence
Length = 698
Score = 167 bits (359), Expect = 9e-041
Identities = 70/164 (42%), Positives = 95/164 (57%)
Frame = +3 / +2
Query: 114 VLHPFASLVTVNDAIWKMYFDELLPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYV 293
VLHP A + +N +W MYF +LLP GDD + D CLQ LS+RIH GK+V
Sbjct: 116 VLHPCADSININKKVWNMYFTDLLPKLAKAGDDDNCGSAAVCDTVCLQALSRRIHYGKFV 295
Query: 294 AEVKFKDAPQEYSRLIKEKDSNSLMDMLTFXXXXXXXXXXXXXXXXTFGQNVTLDDNATA 473
AE KF+++ EY IK +D LM++LT+ +GQ + A
Sbjct: 296 AEAKFQESTAEYEAAIKVQDRARLMELLTYETVEAAVKKRVEMKTRKYGQEGRITQQEDA 475
Query: 474 GDSECKVDPKVLSKLYDQWVMPLTKDVEVEYLLRRLD*SVITRL 605
D KV+P ++++LY+ W+MPLTK+VEVEYLLRRLD*S R+
Sbjct: 476 ADPIYKVEPHLVAQLYEDWIMPLTKEVEVEYLLRRLD*SEAMRV 607
>gb|CN549936.1|CN549936 GQ0243.B3_L22 GQ024 Populus trichocarpa x Populus deltoides cDNA
clone GQ0243_L22 5', mRNA sequence
Length = 668
Score = 137 bits (294), Expect = 8e-032
Identities = 54/126 (42%), Positives = 75/126 (59%)
Frame = +3 / +3
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E E L+A+A Y+ P++ PFFP++LP + PKVLHP A + +N +W MYF +L
Sbjct: 246 ETERLHAQAGRYRSPDEHPFFPENLPHSMLLPSQYPKVLHPCADSININKKVWNMYFTDL 425
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNS 362
LP GDD + D CLQ LS+RIH GK+VAE KF+++ EY +D
Sbjct: 426 LPKLAKAGDDDNCGSAAVCDTVCLQALSRRIHYGKFVAEAKFQESTAEYEAAXXXQDRAX 605
Query: 363 LMDMLT 380
LM++LT
Sbjct: 606 LMELLT 623
>gb|BU836172.1|BU836172 T083E02 Populus apical shoot cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 583
Score = 128 bits (274), Expect = 5e-029
Identities = 52/122 (42%), Positives = 75/122 (61%)
Frame = +3 / +2
Query: 228 TVALDLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLMDMLTFXXXXXXXX 407
T D CLQ LS+RIH G+YVAE KF+ +P +Y I+ +DS LM++LT+
Sbjct: 32 TAVCDTICLQALSKRIHYGEYVAEAKFRASPDDYEAAIRAQDSKRLMEILTYPAVEEAVK 211
Query: 408 XXXXXXXXTFGQNVTLDDNATAGDSECKVDPKVLSKLYDQWVMPLTKDVEVEYLLRRLD* 587
FGQ VT++ D K+ P +++ LY +W+MPLTK+V+V+YLLRRLD*
Sbjct: 212 KRVEMKAKAFGQEVTMEGEIDGTDPVYKIKPTLVADLYGEWIMPLTKEVQVQYLLRRLD* 391
Query: 588 SV 593
++
Sbjct: 392 AL 397
>gb|DN492268.1|DN492268 V059F02.3pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V059F02 3', mRNA sequence
Length = 618
Score = 122 bits (260), Expect = 4e-027
Identities = 50/118 (42%), Positives = 73/118 (61%)
Frame = +3 / -1
Query: 240 DLACLQVLSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLMDMLTFXXXXXXXXXXXX 419
D CLQ LS+RIH G++VAE KF+ +P +Y I+ DS LM++LT+
Sbjct: 612 DTICLQALSKRIHYGEFVAEAKFRASPDDYEAAIRA*DSKRLMEILTYPAVEEAVKKRVE 433
Query: 420 XXXXTFGQNVTLDDNATAGDSECKVDPKVLSKLYDQWVMPLTKDVEVEYLLRRLD*SV 593
FGQ VT++ D K+ P +++ LY +W+MPLTK+V+V+YLLRRLD*++
Sbjct: 432 MKAKAFGQEVTMEGEIDGTDPVYKIRPTLVADLYGEWIMPLTKEVQVQYLLRRLD*AL 259
>gb|CN523227.1|CN523227 GQ015M01.B3_J05 GQ015 Populus trichocarpa x Populus deltoides cDNA
clone GQ015M01_J05 5', mRNA sequence
Length = 813
Score = 113 bits (241), Expect = 2e-024
Identities = 46/86 (53%), Positives = 57/86 (66%)
Frame = +3 / +3
Query: 3 EAEALNAKAXHYQKPEDVPFFPQDLPSPLFPTKPSPKVLHPFASLVTVNDAIWKMYFDEL 182
E EA+ AKA Y PE+ PFFP +LP L P +VLH + + +N AIW MY ++L
Sbjct: 357 ETEAVQAKAGRYVNPEENPFFPDNLPPSLVPNYNYSQVLHTGGASININKAIWDMYLNQL 536
Query: 183 LPLFTVDGDDGSYAQTVALDLACLQV 260
LPLF V GDDG+YA T A DL+CLQV
Sbjct: 537 LPLFVVAGDDGNYASTAASDLSCLQV 614
>gb|BU879394.1|BU879394 V059F02 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 626
Score = 111 bits (238), Expect = 4e-024
Identities = 46/111 (41%), Positives = 69/111 (62%)
Frame = +3 / +3
Query: 261 LSQRIHIGKYVAEVKFKDAPQEYSRLIKEKDSNSLMDMLTFXXXXXXXXXXXXXXXXTFG 440
LS+RIH G++VAE KF+ +P +Y I+ DS LM++LT+ FG
Sbjct: 24 LSKRIHYGEFVAEAKFRASPDDYEAAIRA*DSKRLMEILTYPAVEEAVKKRVEMKAKAFG 203
Query: 441 QNVTLDDNATAGDSECKVDPKVLSKLYDQWVMPLTKDVEVEYLLRRLD*SV 593
Q VT++ D K+ P +++ LY +W+MPLTK+V+V+YLLRRLD*++
Sbjct: 204 QEVTMEGEIDGTDPVYKIRPTLVADLYGEWIMPLTKEVQVQYLLRRLD*AL 356
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 419,315,108
Number of Sequences: 369679
Number of extensions: 6096498
Number of successful extensions: 46833
Number of sequences better than 1.0e-020: 14
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 10930405500
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)