TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950225.2.1
(3330 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX182279.1|CX182279 G09_45-117_13.ab1 leaf inoculated wi... 173 2e-069
gb|BU887462.1|BU887462 R061D12 Populus root cDNA library Po... 162 1e-057
gb|CX183006.1|CX183006 F11_45-25_11.ab1 leaf inoculated wit... 144 4e-054
gb|CX170373.1|CX170373 E02_69-104_10.ab1 leaf inoculated wi... 133 2e-050
emb|AJ937747.1| Populus x Canadensis mRNA for osmosensor hi... 94 3e-047
emb|AJ319875.1|PEU319875 Populus euramericana partial mRNA ... 94 3e-030
gb|CK103987.1|CK103987 I008P96.5pR Populus senescing leaves... 71 2e-026
gb|BI122552.1|BI122552 I008P96P Populus leaf cDNA library P... 85 2e-026
gb|AJ780693.1|AJ780693 AJ780693 Populus euphratica leaf 3-6... 74 6e-025
gb|BU810146.1|BU810146 UL66H11 Populus leaf cDNA library Po... 84 9e-022
>gb|CX182279.1|CX182279 G09_45-117_13.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 685
Score = 173 bits (373), Expect(3) = 2e-069
Identities = 68/107 (63%), Positives = 95/107 (88%)
Frame = -2 / +1
Query: 2075 QKDYAQTAQMCGRALITLINDVLDRAKIEAGKLELEAVPFDLRSLMDDVVSLFSSKSREK 1896
Q+DY +TAQ G+AL++LIN+VLD+AKIE+GK+ELE + FDLR++MDDV++LFS K+ EK
Sbjct: 34 QQDYVRTAQDSGKALVSLINEVLDQAKIESGKIELEEMQFDLRAIMDDVLALFSGKAHEK 213
Query: 1895 CIELAVFVCDNVPKVVIGDPWRFRQILTNLVGNAVKFTERGHVFVRV 1755
IELAV+V D VP+++IGDP RFRQI+TNL+GN++KFT++GH+F+ V
Sbjct: 214 GIELAVYVSDGVPEMLIGDPGRFRQIITNLMGNSIKFTKKGHIFLTV 354
Score = 102 bits (218), Expect(3) = 2e-069
Identities = 44/59 (74%), Positives = 47/59 (79%)
Frame = -2 / +1
Query: 1538 SDSDRVTLAISIEDTGVGIPLQAQDRVFTPFMQADSSTSRNYGGTGIGLSISKCLAELM 1362
S SD V L +S+EDTG GIPL+AQ RVFTPFMQ D S SR YGGTGIGLSISKCL LM
Sbjct: 508 SSSDLVNLIVSVEDTGEGIPLEAQPRVFTPFMQVDPSISRIYGGTGIGLSISKCLVGLM 684
Score = 87.2 bits (184), Expect(3) = 4e-026
Identities = 46/90 (51%), Positives = 54/90 (60%)
Frame = +1 / -3
Query: 1774 PRSVNLTAFPTKFVSICRNLQGSPITTFGTLSHTNTASSMHFSRDFEENKETTSSMRERR 1953
P VNL FP + V ICRNL GSPI+ GT S T TA+S+ FS E TSS+ +
Sbjct: 335 PFFVNLIEFPMRLVIICRNLPGSPISISGTPSDT*TANSIPFS*ALPEKSANTSSIIALK 156
Query: 1954 SKGTASSSNFPASIFARSRTSFISVINALP 2043
S +SSS P SIFA S+TSFIS A P
Sbjct: 155 SNCISSSSILPDSIFA*SKTSFISETRAFP 66
Score = 46.4 bits (95), Expect(3) = 4e-026
Identities = 21/37 (56%), Positives = 25/37 (67%)
Frame = +1 / -3
Query: 1423 EVELSACINGVKTRSCACSGIPTPVSSILIASVTRSE 1533
E++ S CI GVKTR A GIP+PVSS +TRSE
Sbjct: 623 EMDGSTCIKGVKTRGWASRGIPSPVSSTDTIRLTRSE 513
Score = 27.6 bits (54), Expect(3) = 2e-069
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -2 / +1
Query: 1673 TANGAFNTLSGFEAADRRNS 1614
T + + NTLSG ADRR S
Sbjct: 394 TESSSLNTLSGLPVADRRTS 453
Score = 24.4 bits (47), Expect(3) = 4e-026
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1 / -3
Query: 1612 QLFRLSAASNPLKVLKAPLAVDSTL 1686
QL RLSA PLKV +V +++
Sbjct: 455 QLVRLSATGKPLKVFSDDDSVSTSI 381
>gb|BU887462.1|BU887462 R061D12 Populus root cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 503
Score = 162 bits (348), Expect(2) = 1e-057
Identities = 65/109 (59%), Positives = 88/109 (80%)
Frame = -2 / +2
Query: 884 PIRASTIASSIQQLLKVEIAERKDNQNRPSFLRSLLVGKNILVVDDNKVNLRVAAAALKK 705
P++AS +A+S+Q+ + ++ ++ ++ LR LLVG+ +L+VDDNKVNL VAAAALKK
Sbjct: 2 PLKASLLAASLQRAMGGDMGNPRNGEHPSLSLRKLLVGRKMLIVDDNKVNLMVAAAALKK 181
Query: 704 YGANVSCVESGKDAISLLQPPHRFDACFMDVQMPEMDGFEATGQIRQME 558
YGA+V C +SG+ AI LL+PPH+FDACFMD+QMPEMDGFEAT +IR ME
Sbjct: 182 YGADVVCADSGQKAIKLLKPPHKFDACFMDIQMPEMDGFEATRRIRDME 328
Score = 83.5 bits (176), Expect(2) = 1e-057
Identities = 33/54 (61%), Positives = 42/54 (77%)
Frame = -2 / +2
Query: 500 AEYHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLVVGTTDS 339
+ +H+P+LAMTADVIQAT EEC + GMDGYVSKPF+ EQLY V R + T+ +
Sbjct: 329 SNWHIPILAMTADVIQATSEECQRCGMDGYVSKPFEAEQLYHEVPRFLHPTSSA 490
Score = 81.3 bits (171), Expect(2) = 4e-027
Identities = 35/87 (40%), Positives = 55/87 (63%)
Frame = +3 / -2
Query: 555 QLHLPYLSGCLKPVHLWHLNIHKTCIEAMRGLK*TDSILAAFNTANISTIFLECSRSNSE 734
+LH+P KP+H HL+IH+ CI+ +R LK D +LA T NIS+I L+ S S E
Sbjct: 331 RLHIPDSPCSFKPIHFRHLDIHEACIKFVRWLKQFDRLLATICTDNISSILLQSSSSYHE 152
Query: 735 VDFIIVYDQYILPNQQASKKRRPVLII 815
V FI++Y+++ N++ +K++ V I
Sbjct: 151 VHFIVIYNKHFSSNKEFAKRQTRVFSI 71
Score = 62.5 bits (130), Expect(2) = 4e-027
Identities = 26/35 (74%), Positives = 28/35 (80%)
Frame = +1 / -3
Query: 382 SCSSSKGLDTYPSIPDFMHSS*VAWITSAVIARTG 486
SCS+S GL+TYPSIP HSS VAWITSAVIA G
Sbjct: 447 SCSASNGLETYPSIPHLWHSSDVAWITSAVIASIG 343
>gb|CX183006.1|CX183006 F11_45-25_11.ab1 leaf inoculated with Marssonia pathogen of Populus
euramericana Populus x canadensis cDNA, mRNA sequence
Length = 620
Score = 144 bits (308), Expect(2) = 4e-054
Identities = 58/78 (74%), Positives = 68/78 (87%)
Frame = -2 / +1
Query: 791 LRSLLVGKNILVVDDNKVNLRVAAAALKKYGANVSCVESGKDAISLLQPPHRFDACFMDV 612
LR LLVG+ +L+VDDNKVNL VAAAALKKYGA+V C +SG+ AI LL+PPH+FDACFMD+
Sbjct: 148 LRKLLVGRKMLIVDDNKVNLMVAAAALKKYGADVVCADSGQRAIKLLKPPHKFDACFMDI 327
Query: 611 QMPEMDGFEATGQIRQME 558
Q PEMDGFEAT +IR ME
Sbjct: 328 QTPEMDGFEATRRIRDME 381
Score = 90.4 bits (191), Expect(2) = 4e-054
Identities = 35/54 (64%), Positives = 44/54 (81%)
Frame = -2 / +1
Query: 500 AEYHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLVVGTTDS 339
+ +H+P+LAMTADVIQATYEEC + GMDGYVSKPF+ EQLY VSR + T+ +
Sbjct: 382 SNWHIPILAMTADVIQATYEECQRCGMDGYVSKPFEAEQLYHEVSRFLQPTSSA 543
Score = 75.3 bits (158), Expect(2) = 1e-025
Identities = 34/86 (39%), Positives = 52/86 (60%)
Frame = +3 / -3
Query: 558 LHLPYLSGCLKPVHLWHLNIHKTCIEAMRGLK*TDSILAAFNTANISTIFLECSRSNSEV 737
LH+P KP+H L+IH+ CI+ +R LK D LA T NIS+I L+ S S EV
Sbjct: 381 LHIPNSPCSFKPIHFRRLDIHEACIKFVRWLKQFDRPLATICTDNISSILLQSSSSYHEV 202
Query: 738 DFIIVYDQYILPNQQASKKRRPVLII 815
FI++Y+++ N++ +K++ V I
Sbjct: 201 HFIVIYNKHFSSNKEFAKRQTRVFSI 124
Score = 63.8 bits (133), Expect(2) = 1e-025
Identities = 28/40 (70%), Positives = 31/40 (77%)
Frame = +1 / -1
Query: 367 ETAWYSCSSSKGLDTYPSIPDFMHSS*VAWITSAVIARTG 486
ET+ SCS+S GL+TYPSIP HSS VAWITSAVIA G
Sbjct: 515 ETS**SCSASNGLETYPSIPHLWHSSYVAWITSAVIASIG 396
>gb|CX170373.1|CX170373 E02_69-104_10.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 610
Score = 133 bits (285), Expect(3) = 2e-050
Identities = 55/83 (66%), Positives = 62/83 (74%)
Frame = -2 / +2
Query: 1538 SDSDRVTLAISIEDTGVGIPLQAQDRVFTPFMQADSSTSRNYGGTGIGLSISKCLAELMG 1359
S SD + L +S+EDTG GIPL+AQ RVFTPFMQ D S SR YGGTGIGLSISKCL LM
Sbjct: 293 SSSDLINLIVSVEDTGEGIPLEAQPRVFTPFMQVDPSISRKYGGTGIGLSISKCLVGLMN 472
Query: 1358 GQISFTSHPSVGSTFTFSATLKH 1290
G I F S P +GSTFTF+A +
Sbjct: 473 GDIGFVSIPDIGSTFTFTAVFSN 541
Score = 79.0 bits (166), Expect(3) = 2e-050
Identities = 29/47 (61%), Positives = 41/47 (87%)
Frame = -2 / +2
Query: 1883 AVFVCDNVPKVVIGDPWRFRQILTNLVGNAVKFTERGHVFVRVCLAE 1743
AV+V D VP+++IGDP RFRQI+TNL+GN++KFT++GH+F+ V L E
Sbjct: 11 AVYVSDGVPEMLIGDPGRFRQIITNLMGNSIKFTKKGHIFLTVHLVE 151
Score = 28.1 bits (55), Expect(3) = 2e-050
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -2 / +2
Query: 1673 TANGAFNTLSGFEAADRRNSWQYF 1602
T + + NTLSG ADR SW F
Sbjct: 179 TESSSRNTLSGLPVADRCRSWVGF 250
>emb|AJ937747.1| Populus x Canadensis mRNA for osmosensor histidine-aspartate kinase
(hk1 gene)
Length = 4210
Score = 94.1 bits (199), Expect(5) = 3e-047
Identities = 38/96 (39%), Positives = 61/96 (63%)
Frame = -2 / +3
Query: 2045 CGRALITLINDVLDRAKIEAGKLELEAVPFDLRSLMDDVVSLFSSKSREKCIELAVFVCD 1866
C AL+ L+N++LD +K+E+GKL LE FDL ++ ++ +FS + +E + + D
Sbjct: 1962 CSTALLRLLNNILDLSKVESGKLVLEDAEFDLGRELEGLIDMFSVQCINHNVEAVLDLSD 2141
Query: 1865 NVPKVVIGDPWRFRQILTNLVGNAVKFTERGHVFVR 1758
+PK+V GD R QI NL+ N++KFT GH+ +R
Sbjct: 2142 EMPKLVRGDSARVVQIFANLISNSIKFTTTGHIILR 2249
Score = 65.2 bits (136), Expect(5) = 3e-047
Identities = 31/79 (39%), Positives = 42/79 (53%)
Frame = -2 / +3
Query: 1529 DRVTLAISIEDTGVGIPLQAQDRVFTPFMQADSSTSRNYGGTGIGLSISKCLAELMGGQI 1350
+++ L I+DTG GI + VF F QAD ST+R +GGTG+GL I + L MGG+I
Sbjct: 2382 NKMILWFEIDDTGCGIDPSKWESVFESFEQADPSTTRLHGGTGLGLCIVRTLVNKMGGEI 2561
Query: 1349 SFTSHPSVGSTFTFSATLK 1293
G+ LK
Sbjct: 2562 KVVKKNGPGTLMRLYLLLK 2618
Score = 46.4 bits (95), Expect(5) = 3e-047
Identities = 15/52 (28%), Positives = 31/52 (59%)
Frame = -2 / +3
Query: 515 EGSTTAEYHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRL 360
+ T + H+P++A+TA + + +C++ GMD Y++KP D + + + L
Sbjct: 3891 KSETGTDLHIPIVALTAHAMSSDEAKCLEVGMDAYLTKPIDYKLMVSTILSL 4046
Score = 41.4 bits (84), Expect(5) = 3e-047
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = -2 / +3
Query: 653 LQPPHRFDACFMDVQMPEMDGFEATGQIRQME 558
+Q +D MD QMP+MDG+EAT IR+ E
Sbjct: 3804 IQESQSYDLILMDCQMPKMDGYEATKAIRKSE 3899
Score = 40.9 bits (83), Expect(5) = 3e-047
Identities = 17/31 (54%), Positives = 23/31 (74%)
Frame = -2 / +3
Query: 2213 ELKTQAEAADVAKSQFLATASHEIRTPMNGV 2121
+ + +AEA++ KSQFLA SHE+RTPM V
Sbjct: 1791 DARRRAEASNNYKSQFLANMSHELRTPMAAV 1883
>emb|AJ319875.1|PEU319875 Populus euramericana partial mRNA for putative osmosensor histidine
kinase (uhk1 gene), clone Dorskamp
Length = 993
Score = 93.6 bits (198), Expect(2) = 3e-030
Identities = 37/96 (38%), Positives = 61/96 (63%)
Frame = -2 / +1
Query: 2045 CGRALITLINDVLDRAKIEAGKLELEAVPFDLRSLMDDVVSLFSSKSREKCIELAVFVCD 1866
C AL+ L+N++LD +K+E+GKL LE FDL ++ ++ +FS + +E + + D
Sbjct: 109 CSTALLRLLNNILDLSKVESGKLVLEDAEFDLGRELEGLIDMFSVQCTNHNVEAVLDLSD 288
Query: 1865 NVPKVVIGDPWRFRQILTNLVGNAVKFTERGHVFVR 1758
+PK++ GD R QI NL+ N++KFT GH+ +R
Sbjct: 289 EMPKLLRGDSARVVQIFANLISNSIKFTTTGHIILR 396
Score = 65.7 bits (137), Expect(2) = 3e-030
Identities = 31/79 (39%), Positives = 43/79 (54%)
Frame = -2 / +1
Query: 1529 DRVTLAISIEDTGVGIPLQAQDRVFTPFMQADSSTSRNYGGTGIGLSISKCLAELMGGQI 1350
+++ L I+DTG GI + + VF F QAD ST+R +GGTG+GL I + L MGG+I
Sbjct: 529 NKMILWFEIDDTGCGIDPRKWESVFESFEQADPSTTRLHGGTGLGLCIVRTLVNKMGGEI 708
Query: 1349 SFTSHPSVGSTFTFSATLK 1293
G+ LK
Sbjct: 709 KVVKKNGPGTLMRLYLLLK 765
>gb|CK103987.1|CK103987 I008P96.5pR Populus senescing leaves cDNA library Populus tremula
cDNA clone I008P96 5', mRNA sequence
Length = 624
Score = 71.2 bits (149), Expect(3) = 2e-026
Identities = 26/34 (76%), Positives = 31/34 (91%)
Frame = -2 / +1
Query: 659 SLLQPPHRFDACFMDVQMPEMDGFEATGQIRQME 558
S ++PPH+FDACFMD+QMPEMDGFEAT +IR ME
Sbjct: 31 SNVKPPHKFDACFMDIQMPEMDGFEATRRIRDME 132
Score = 58.8 bits (122), Expect(3) = 2e-026
Identities = 22/31 (70%), Positives = 27/31 (87%)
Frame = -2 / +1
Query: 500 AEYHLPVLAMTADVIQATYEECIKSGMDGYV 408
+ +H+P+LAMTADVIQAT EEC + GMDGYV
Sbjct: 133 SNWHIPILAMTADVIQATSEECQRCGMDGYV 225
Score = 29.5 bits (58), Expect(3) = 2e-026
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -2 / +3
Query: 407 SKPFDEEQLYQAVSRLVVGTTDS 339
SKPF+ EQLY VSR + T+ +
Sbjct: 225 SKPFEAEQLYLEVSRFLQPTSSA 293
>gb|BI122552.1|BI122552 I008P96P Populus leaf cDNA library Populus tremula x Populus
tremuloides cDNA, mRNA sequence
Length = 456
Score = 85.4 bits (180), Expect(3) = 2e-026
Identities = 34/54 (62%), Positives = 43/54 (79%)
Frame = -2 / +2
Query: 500 AEYHLPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLVVGTTDS 339
+ +H+P+LAMTADVIQAT EEC + GMDGYVSKPF+ EQLY VSR + T+ +
Sbjct: 101 SNWHIPILAMTADVIQATSEECQRCGMDGYVSKPFEAEQLYLEVSRFLQPTSSA 262
Score = 39.6 bits (80), Expect(3) = 2e-026
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -2 / +2
Query: 614 VQMPEMDGFEATGQIRQME 558
+QMPEMDGFEAT +IR ME
Sbjct: 44 IQMPEMDGFEATRRIRDME 100
Score = 34.5 bits (69), Expect(3) = 2e-026
Identities = 11/13 (84%), Positives = 13/13 (100%)
Frame = -2 / +1
Query: 656 LLQPPHRFDACFM 618
LL+PPH+FDACFM
Sbjct: 1 LLKPPHKFDACFM 39
>gb|AJ780693.1|AJ780693 AJ780693 Populus euphratica leaf 3-6 months Populus euphratica cDNA
clone P0000900011C09F1, mRNA sequence
Length = 290
Score = 74.4 bits (156), Expect(2) = 6e-025
Identities = 28/45 (62%), Positives = 34/45 (75%)
Frame = -2 / +3
Query: 2411 HVSMLDFGDPFRKHEMRCRYRQKLPMPWSAITNPLGTFVIWMLLG 2277
HVS L+ DPFRKHEMRCR++QK P PW AIT +G VI +L+G
Sbjct: 156 HVSSLNLEDPFRKHEMRCRFKQKPPWPWLAITTSIGILVIALLIG 290
Score = 62.0 bits (129), Expect(2) = 6e-025
Identities = 26/48 (54%), Positives = 33/48 (68%)
Frame = -2 / +3
Query: 2570 LGGAFDVESLVENLLSKLAGNQDIVVNVYDVTNASDAMVLYGPSSLDE 2427
LGG FD+ESLVE LL +LA Q I+VNVYD TN + +YG + D+
Sbjct: 3 LGGIFDIESLVEKLLQQLASKQTILVNVYDTTNQYHPISMYGSNVSDD 146
>gb|BU810146.1|BU810146 UL66H11 Populus leaf cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 415
Score = 83.5 bits (176), Expect(2) = 9e-022
Identities = 33/50 (66%), Positives = 42/50 (84%)
Frame = -2 / +1
Query: 488 LPVLAMTADVIQATYEECIKSGMDGYVSKPFDEEQLYQAVSRLVVGTTDS 339
+P+LAMTADVIQATYE+C + GMDGYVSKPF+ EQLY VSR + T+++
Sbjct: 73 IPILAMTADVIQATYEQCQRCGMDGYVSKPFEAEQLYLEVSRFLQPTSNA 222
Score = 42.3 bits (86), Expect(2) = 9e-022
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = -2 / +1
Query: 617 DVQMPEMDGFEATGQIRQME 558
D+QMPEMDGFEAT +IR ME
Sbjct: 1 DIQMPEMDGFEATRRIRDME 60
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,672,757,932
Number of Sequences: 369679
Number of extensions: 25470308
Number of successful extensions: 103657
Number of sequences better than 1.0e-020: 11
length of database: 67,802,888
effective HSP length: 56
effective length of database: 47,100,864
effective search space used: 49597209792
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)