TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750560.2.1
(660 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV244991.1|CV244991 WS0256.B21_D04 PT-MB-N-A-15 Populus ... 159 8e-054
gb|CV256321.1|CV256321 WS0243.B21_I14 PTxD-ICC-N-A-14 Popul... 159 8e-054
gb|DT472940.1|DT472940 WS01228.BR_B04 PT-GT-FL-A-3 Populus ... 80 7e-034
gb|CK091794.1|CK091794 F126P27.3pR Populus flower cDNA libr... 74 3e-032
gb|CK091682.1|CK091682 F118P18.3pR Populus flower cDNA libr... 74 3e-032
gb|CK090590.1|CK090590 F014P57.3pR Populus flower cDNA libr... 74 3e-032
gb|CK090594.1|CK090594 F014P75.3pR Populus flower cDNA libr... 74 3e-032
gb|DV463887.1|DV463887 MTUNUL1.P20.F06 NUL Populus fremonti... 126 2e-028
gb|BI138460.1|BI138460 F106P90Y Populus flower cDNA library... 68 1e-025
>gb|CV244991.1|CV244991 WS0256.B21_D04 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS0256_D04 3', mRNA sequence
Length = 927
Score = 159 bits (341), Expect(2) = 8e-054
Identities = 63/87 (72%), Positives = 74/87 (85%)
Frame = +3 / -3
Query: 264 DALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCK 443
DAL++ELWHACAGPLVT+P +GE VYYFPQGHMEQLEAS Q ++Q +P F+LP KILCK
Sbjct: 922 DALYKELWHACAGPLVTLPCEGERVYYFPQGHMEQLEASMHQGMEQQMPSFNLPSKILCK 743
Query: 444 VVNVELRAETDSDEVYAQIMLQPEADQ 524
VVNV+ RAE ++DEVYAQI L PE DQ
Sbjct: 742 VVNVQRRAEPETDEVYAQITLLPEPDQ 662
Score = 113 bits (241), Expect = 1e-024
Identities = 58/129 (44%), Positives = 76/129 (58%)
Frame = -2 / +2
Query: 650 MPPEDRESTMGTCI*SSQGLAEGIDVASFRLRWLRIWAGGLTLICFWLQHNLSINFIRIS 471
MP ++RE+TM C+ SS+ LA+ +D ASFR RI L LI F Q L INFI
Sbjct: 536 MPSKNRETTMSACVRSSKCLAK*MDRASFRFWEWRIRTSNLALIWFRKQCYLGINFICFR 715
Query: 470 FSSKFYIDHLAENLGW*IKQR*VLIKLLICRCFKLFHMTLWEVVNQLALARHCDQRPSTS 291
FS I+H +N W +K +L+ L+ CFKLFH+TL +++N L R D+R ST
Sbjct: 716 FSPALNINHFTQNFRWKVK*GHLLLHPLMH*CFKLFHVTLRKIINSLPFTRKSDKRSSTG 895
Query: 290 MPEFPEQSI 264
MP F QSI
Sbjct: 896 MP*FFVQSI 922
Score = 71.6 bits (150), Expect(2) = 8e-054
Identities = 27/31 (87%), Positives = 30/31 (96%)
Frame = +3 / -3
Query: 567 RCNVYSFCKTLTASDTSTHGGFSVLRRHAEE 659
RC V+SFCKTLTASDTSTHGGFSVLRRHA++
Sbjct: 619 RCTVHSFCKTLTASDTSTHGGFSVLRRHADD 527
>gb|CV256321.1|CV256321 WS0243.B21_I14 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0243_I14 3', mRNA sequence
Length = 898
Score = 159 bits (341), Expect(2) = 8e-054
Identities = 63/87 (72%), Positives = 74/87 (85%)
Frame = +3 / +1
Query: 264 DALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCK 443
DAL++ELWHACAGPLVT+P +GE VYYFPQGHMEQLEAS Q ++Q +P F+LP KILCK
Sbjct: 142 DALYKELWHACAGPLVTLPCEGERVYYFPQGHMEQLEASMHQGMEQQMPSFNLPSKILCK 321
Query: 444 VVNVELRAETDSDEVYAQIMLQPEADQ 524
VVNV+ RAE ++DEVYAQI L PE DQ
Sbjct: 322 VVNVQRRAEPETDEVYAQITLLPEPDQ 402
Score = 116 bits (249), Expect = 1e-025
Identities = 59/133 (44%), Positives = 79/133 (59%)
Frame = -2 / -2
Query: 650 MPPEDRESTMGTCI*SSQGLAEGIDVASFRLRWLRIWAGGLTLICFWLQHNLSINFIRIS 471
MP ++RE+TM C+ SS+ LA+ +D ASFR RI L LI F Q L INFI
Sbjct: 528 MPSKNRETTMSACVRSSKCLAK*MDRASFRFWEWRIRTSNLALIWFRKQCYLGINFICFR 349
Query: 470 FSSKFYIDHLAENLGW*IKQR*VLIKLLICRCFKLFHMTLWEVVNQLALARHCDQRPSTS 291
FS I+H +N W +K +L+ L+ CFKLFH+TL +++N L R D+R ST
Sbjct: 348 FSPALNINHFTQNFRWKVK*GHLLLHPLMH*CFKLFHVTLRKIINSLPFTRKSDKRSSTG 169
Query: 290 MPEFPEQSIASTP 252
MP F QSI ++P
Sbjct: 168 MP*FFVQSIIASP 130
Score = 71.6 bits (150), Expect(2) = 8e-054
Identities = 27/31 (87%), Positives = 30/31 (96%)
Frame = +3 / +1
Query: 567 RCNVYSFCKTLTASDTSTHGGFSVLRRHAEE 659
RC V+SFCKTLTASDTSTHGGFSVLRRHA++
Sbjct: 445 RCTVHSFCKTLTASDTSTHGGFSVLRRHADD 537
>gb|DT472940.1|DT472940 WS01228.BR_B04 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS01228_B04 5', mRNA sequence
Length = 914
Score = 80.3 bits (169), Expect(3) = 7e-034
Identities = 29/50 (58%), Positives = 38/50 (76%)
Frame = +3 / +3
Query: 279 ELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPP 428
ELWHACAGPLV++P G V YFPQGH EQ+ AST++++D H+P + P
Sbjct: 90 ELWHACAGPLVSLPHVGSRVVYFPQGHSEQVAASTNKEVDAHIPNYPSLP 239
Score = 53.8 bits (111), Expect(3) = 7e-034
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +3 / +3
Query: 585 FCKTLTASDTSTHGGFSVLRRHAEE 659
FCKTLTASDTSTHGGFSV RR AE+
Sbjct: 405 FCKTLTASDTSTHGGFSVPRRAAEK 479
Score = 47.8 bits (98), Expect(3) = 7e-034
Identities = 16/31 (51%), Positives = 26/31 (83%)
Frame = +3 / +3
Query: 420 LPPKILCKVVNVELRAETDSDEVYAQIMLQP 512
LPP+++C++ NV + A+ ++DEVYAQ+ LQP
Sbjct: 234 LPPQLICQLHNVTMHADVETDEVYAQMTLQP 326
>gb|CK091794.1|CK091794 F126P27.3pR Populus flower cDNA library Populus trichocarpa cDNA
clone F126P27 3', mRNA sequence
Length = 860
Score = 73.9 bits (155), Expect(3) = 3e-032
Identities = 29/57 (50%), Positives = 38/57 (66%)
Frame = +3 / +3
Query: 267 ALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKIL 437
A+ ELW+ACAGPLV++P+ G LVYYFPQGH EQ+ ST + +P + P L
Sbjct: 48 AINSELWYACAGPLVSLPQVGSLVYYFPQGHSEQVAVSTRRSATSQIPNYPNLPSQL 218
Score = 53.8 bits (111), Expect(3) = 3e-032
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +3 / +3
Query: 585 FCKTLTASDTSTHGGFSVLRRHAEE 659
FCKTLTASDTSTHGGFSV RR AE+
Sbjct: 369 FCKTLTASDTSTHGGFSVPRRAAEK 443
Score = 48.7 bits (100), Expect(3) = 3e-032
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +3 / +3
Query: 417 DLPPKILCKVVNVELRAETDSDEVYAQIMLQP 512
+LP ++LC+V NV L A+ D+DE+YAQ+ LQP
Sbjct: 201 NLPSQLLCQVHNVTLHADKDTDEIYAQMSLQP 296
>gb|CK091682.1|CK091682 F118P18.3pR Populus flower cDNA library Populus trichocarpa cDNA
clone F118P18 3', mRNA sequence
Length = 828
Score = 73.9 bits (155), Expect(3) = 3e-032
Identities = 29/57 (50%), Positives = 38/57 (66%)
Frame = +3 / +3
Query: 267 ALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKIL 437
A+ ELW+ACAGPLV++P+ G LVYYFPQGH EQ+ ST + +P + P L
Sbjct: 48 AINSELWYACAGPLVSLPQVGSLVYYFPQGHSEQVAVSTRRSATSQIPNYPNLPSQL 218
Score = 53.8 bits (111), Expect(3) = 3e-032
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +3 / +3
Query: 585 FCKTLTASDTSTHGGFSVLRRHAEE 659
FCKTLTASDTSTHGGFSV RR AE+
Sbjct: 369 FCKTLTASDTSTHGGFSVPRRAAEK 443
Score = 48.7 bits (100), Expect(3) = 3e-032
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +3 / +3
Query: 417 DLPPKILCKVVNVELRAETDSDEVYAQIMLQP 512
+LP ++LC+V NV L A+ D+DE+YAQ+ LQP
Sbjct: 201 NLPSQLLCQVHNVTLHADKDTDEIYAQMSLQP 296
>gb|CK090590.1|CK090590 F014P57.3pR Populus flower cDNA library Populus trichocarpa cDNA
clone F014P57 3', mRNA sequence
Length = 817
Score = 73.9 bits (155), Expect(3) = 3e-032
Identities = 29/57 (50%), Positives = 38/57 (66%)
Frame = +3 / +3
Query: 267 ALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKIL 437
A+ ELW+ACAGPLV++P+ G LVYYFPQGH EQ+ ST + +P + P L
Sbjct: 48 AINSELWYACAGPLVSLPQVGSLVYYFPQGHSEQVAVSTRRSATSQIPNYPNLPSQL 218
Score = 53.8 bits (111), Expect(3) = 3e-032
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +3 / +3
Query: 585 FCKTLTASDTSTHGGFSVLRRHAEE 659
FCKTLTASDTSTHGGFSV RR AE+
Sbjct: 369 FCKTLTASDTSTHGGFSVPRRAAEK 443
Score = 48.7 bits (100), Expect(3) = 3e-032
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +3 / +3
Query: 417 DLPPKILCKVVNVELRAETDSDEVYAQIMLQP 512
+LP ++LC+V NV L A+ D+DE+YAQ+ LQP
Sbjct: 201 NLPSQLLCQVHNVTLHADKDTDEIYAQMSLQP 296
>gb|CK090594.1|CK090594 F014P75.3pR Populus flower cDNA library Populus trichocarpa cDNA
clone F014P75 3', mRNA sequence
Length = 806
Score = 73.9 bits (155), Expect(3) = 3e-032
Identities = 29/57 (50%), Positives = 38/57 (66%)
Frame = +3 / +3
Query: 267 ALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKIL 437
A+ ELW+ACAGPLV++P+ G LVYYFPQGH EQ+ ST + +P + P L
Sbjct: 48 AINSELWYACAGPLVSLPQVGSLVYYFPQGHSEQVAVSTRRSATSQIPNYPNLPSQL 218
Score = 53.8 bits (111), Expect(3) = 3e-032
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +3 / +3
Query: 585 FCKTLTASDTSTHGGFSVLRRHAEE 659
FCKTLTASDTSTHGGFSV RR AE+
Sbjct: 369 FCKTLTASDTSTHGGFSVPRRAAEK 443
Score = 48.7 bits (100), Expect(3) = 3e-032
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +3 / +3
Query: 417 DLPPKILCKVVNVELRAETDSDEVYAQIMLQP 512
+LP ++LC+V NV L A+ D+DE+YAQ+ LQP
Sbjct: 201 NLPSQLLCQVHNVTLHADKDTDEIYAQMSLQP 296
>gb|DV463887.1|DV463887 MTUNUL1.P20.F06 NUL Populus fremontii x Populus angustifolia cDNA,
mRNA sequence
Length = 718
Score = 126 bits (269), Expect = 2e-028
Identities = 53/90 (58%), Positives = 65/90 (72%)
Frame = +3 / +3
Query: 390 QLDQHLPLFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQXXXXXXXXXXXXXXR 569
+L+Q +PLF+LP KILC+V++ +L AE D+DEVYAQI L PE+DQ R
Sbjct: 30 ELNQRVPLFNLPSKILCRVIHTQLLAEQDTDEVYAQITLIPESDQIEPTSPDSSSSEPPR 209
Query: 570 CNVYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V+SFCK LTASDTSTHGGFSVLR+HA E
Sbjct: 210 PTVHSFCKVLTASDTSTHGGFSVLRKHATE 299
>gb|BI138460.1|BI138460 F106P90Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA
sequence
Length = 499
Score = 68.4 bits (143), Expect(2) = 1e-025
Identities = 27/31 (87%), Positives = 30/31 (96%)
Frame = +3 / +3
Query: 567 RCNVYSFCKTLTASDTSTHGGFSVLRRHAEE 659
R +V+SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 174 RFHVHSFCKTLTASDTSTHGGFSVLRRHADE 266
Score = 68.0 bits (142), Expect(2) = 1e-025
Identities = 26/42 (61%), Positives = 36/42 (85%)
Frame = +3 / +3
Query: 399 QHLPLFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
Q +PL++L PKILC+VVNV+L+AE D+DEV+AQ+ L PE +Q
Sbjct: 3 QQMPLYNLLPKILCRVVNVQLKAEPDTDEVFAQVTLLPEHNQ 128
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 284,480,940
Number of Sequences: 369679
Number of extensions: 3433401
Number of successful extensions: 40387
Number of sequences better than 1.0e-020: 9
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8223475512
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)