TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750436.2.1
(656 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI784491.1|BI784491 UNIAB04P70X Hybrid aspen plasmid lib... 154 7e-037
gb|CK093245.1|CK093245 G113P10.3pR Populus tension wood cDN... 133 1e-030
gb|CX282996.1|CX282996 24-2.2.3 Populus tremula X Populus a... 129 1e-029
gb|CA825793.1|CA825793 R67F02 two-month-old roots from clon... 89 1e-028
gb|CK098265.1|CK098265 A009P80.5pR Hybrid aspen plasmid lib... 124 6e-028
gb|BU891968.1|BU891968 P057F09 Populus petioles cDNA librar... 121 4e-027
gb|AJ534481.1|AJ534481 AJ534481 Populus euphratica root and... 121 4e-027
gb|CV261342.1|CV261342 WS02016.B21_I08 PTxN-IB-N-A-11 Popul... 121 4e-027
gb|CV278214.1|CV278214 WS0145.B21_M14 PTxD-IL-A-5 Populus t... 121 4e-027
gb|DT487402.1|DT487402 WS02533.B21_J15 PT-MB-N-A-15 Populus... 99 7e-025
gb|CF234274.1|CF234274 PtaJXO4E7E0709 Poplar cDNA library f... 101 5e-021
>gb|BI784491.1|BI784491 UNIAB04P70X Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA 5', mRNA sequence
Length = 457
Score = 154 bits (330), Expect = 7e-037
Identities = 65/95 (68%), Positives = 76/95 (80%)
Frame = -1 / +3
Query: 629 GEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLD 450
GEE L A+ EHGKG VL V EIQ R DAA+E+E+SLLELHQVFLDMAVMVE QGE++D
Sbjct: 39 GEEFLKRAIQEHGKGKVLETVVEIQDRHDAAKEIEKSLLELHQVFLDMAVMVEAQGEQMD 218
Query: 449 DIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
DIE HV ASHYV+ G KEL AK++Q+SSRK +C
Sbjct: 219 DIEHHVLKASHYVKDGTKELKGAKDHQKSSRKWMC 323
>gb|CK093245.1|CK093245 G113P10.3pR Populus tension wood cDNA library Populus tremula x
Populus tremuloides cDNA clone G113P10 3', mRNA sequence
Length = 531
Score = 133 bits (285), Expect = 1e-030
Identities = 57/83 (68%), Positives = 66/83 (79%)
Frame = -1 / -3
Query: 593 GKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHVANASHY 414
GKG VL V EIQ R DAA+E+E+SLLELHQVFLDMAVMVE QGE++DDIE HV ASHY
Sbjct: 529 GKGKVLETVVEIQDRHDAAKEIEKSLLELHQVFLDMAVMVEAQGEQMDDIEPHVLKASHY 350
Query: 413 VQGGNKELGKAKEYQRSSRKCLC 345
V+ G KEL K+ Q+SSRK +C
Sbjct: 349 VKDGTKELKGVKDPQKSSRKWMC 281
>gb|CX282996.1|CX282996 24-2.2.3 Populus tremula X Populus alba developing vegetative bud
cDNA Library Populus alba x Populus tremula cDNA clone
532, mRNA sequence
Length = 308
Score = 129 bits (277), Expect = 1e-029
Identities = 52/69 (75%), Positives = 60/69 (86%)
Frame = -1 / +3
Query: 551 RRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHVANASHYVQGGNKELGKAKEY 372
R DAA+E+E+SLLELHQVFLDMAVMVE QGE++DDIE HV NASHYV+ G KEL AK Y
Sbjct: 6 RHDAAKEIEKSLLELHQVFLDMAVMVEAQGEQMDDIEHHVLNASHYVKDGTKELKSAKGY 185
Query: 371 QRSSRKCLC 345
Q+SSRKC+C
Sbjct: 186 QKSSRKCMC 212
>gb|CA825793.1|CA825793 R67F02 two-month-old roots from clone 'Beaupre' Populus trichocarpa
x Populus deltoides cDNA 5', mRNA sequence
Length = 627
Score = 89.0 bits (188), Expect(2) = 1e-028
Identities = 39/53 (73%), Positives = 43/53 (81%)
Frame = -1 / +2
Query: 629 GEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVE 471
GEE L A+ EHGKG VL V EIQ R DAA+E+E+SLLELHQVFLDMAVMVE
Sbjct: 335 GEEFLKRAIQEHGKGKVLETVVEIQDRYDAAKEIEKSLLELHQVFLDMAVMVE 493
Score = 57.4 bits (119), Expect(2) = 1e-028
Identities = 22/40 (55%), Positives = 29/40 (72%)
Frame = -1 / +3
Query: 464 GEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
GE++DD E HV NA HYV+ G KEL A+++Q SSR +C
Sbjct: 501 GEQMDDXEHHV*NAXHYVKDGTKELKSARDHQXSSRXWMC 620
>gb|CK098265.1|CK098265 A009P80.5pR Hybrid aspen plasmid library Populus tremula x Populus
tremuloides cDNA clone A009P80 5', mRNA sequence
Length = 463
Score = 124 bits (265), Expect = 6e-028
Identities = 51/72 (70%), Positives = 61/72 (84%)
Frame = -1 / +3
Query: 560 IQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHVANASHYVQGGNKELGKA 381
IQ R DAA+E+E+SLLELHQVFLDMAVMVE QGE++DDIE HV ASHYV+ G KEL A
Sbjct: 114 IQDRHDAAKEIEKSLLELHQVFLDMAVMVEAQGEQMDDIEHHVLKASHYVKDGTKELKGA 293
Query: 380 KEYQRSSRKCLC 345
K++Q+SSRK +C
Sbjct: 294 KDHQKSSRKWMC 329
>gb|BU891968.1|BU891968 P057F09 Populus petioles cDNA library Populus tremula cDNA 5 prime,
mRNA sequence
Length = 610
Score = 121 bits (259), Expect = 4e-027
Identities = 51/104 (49%), Positives = 72/104 (69%)
Frame = -1 / +2
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I E++ A+ E G+G + + EIQ R DA R++ER LL+L Q+FLDMAV+
Sbjct: 50 IDTLIETGDSEQIFQKAIQEQGRGQITDTLAEIQERHDAVRDLERKLLDLQQIFLDMAVL 229
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
V+ QG+ LD+IES V+NA +VQ GN L KAK+ QR+SRK +C
Sbjct: 230 VDAQGDMLDNIESQVSNAVDHVQSGNVALTKAKKLQRNSRKWMC 361
>gb|AJ534481.1|AJ534481 AJ534481 Populus euphratica root and shoot Populus euphratica cDNA
clone 46, mRNA sequence
Length = 574
Score = 121 bits (259), Expect = 4e-027
Identities = 51/104 (49%), Positives = 72/104 (69%)
Frame = -1 / -1
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I E++ A+ E G+G + + EIQ R DA R++ER LL+L Q+FLDMAV+
Sbjct: 418 IDALIETGDSEQIFQKAIQEQGRGQITDTLAEIQERHDAVRDLERKLLDLQQIFLDMAVL 239
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
V+ QG+ LD+IES V+NA +VQ GN L KAK+ QR+SRK +C
Sbjct: 238 VDAQGDMLDNIESQVSNAVDHVQSGNVALQKAKKLQRNSRKWMC 107
>gb|CV261342.1|CV261342 WS02016.B21_I08 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02016_I08 3', mRNA sequence
Length = 933
Score = 121 bits (259), Expect = 4e-027
Identities = 51/104 (49%), Positives = 72/104 (69%)
Frame = -1 / -2
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I E++ A+ E G+G + + EIQ R DA R++ER LL+L Q+FLDMAV+
Sbjct: 629 IDTLIETGDSEQIFQKAIQEQGRGQITDTLAEIQERHDAVRDLERKLLDLQQIFLDMAVL 450
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
V+ QG+ LD+IES V+NA +VQ GN L KAK+ QR+SRK +C
Sbjct: 449 VDAQGDMLDNIESQVSNAVDHVQSGNVALQKAKKLQRNSRKWMC 318
>gb|CV278214.1|CV278214 WS0145.B21_M14 PTxD-IL-A-5 Populus trichocarpa x Populus deltoides
cDNA clone WS0145_M14 3', mRNA sequence
Length = 524
Score = 121 bits (259), Expect = 4e-027
Identities = 51/104 (49%), Positives = 72/104 (69%)
Frame = -1 / -1
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I E++ A+ E G+G + + EIQ R DA R++ER LL+L Q+FLDMAV+
Sbjct: 509 IDTLIETGDSEQIFQKAIQEQGRGQITDTLAEIQERHDAVRDLERKLLDLQQIFLDMAVL 330
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
V+ QG+ LD+IES V+NA +VQ GN L KAK+ QR+SRK +C
Sbjct: 329 VDAQGDMLDNIESQVSNAVDHVQSGNVALQKAKKLQRNSRKWMC 198
>gb|DT487402.1|DT487402 WS02533.B21_J15 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02533_J15 3', mRNA sequence
Length = 953
Score = 98.7 bits (209), Expect(2) = 7e-025
Identities = 40/78 (51%), Positives = 56/78 (71%)
Frame = -1 / -2
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
++ +IS E L A+ + G+G +L ++EIQ R DA +++E +L ELHQVF+DMAV+
Sbjct: 871 LDLLISTGESETFLQKAIQQQGRGRILDTINEIQERHDAVKDLENNLKELHQVFMDMAVL 692
Query: 476 VETQGEKLDDIESHVANA 423
VE QGE+LDDIESHV A
Sbjct: 691 VEHQGEQLDDIESHVQRA 638
Score = 35.0 bits (70), Expect(2) = 7e-025
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = -1 / -1
Query: 431 ANASHYVQGGNKELGKAKEYQRSSRKCLC 345
A ++ YV+GG ++L A++ QR+SRK C
Sbjct: 647 AKSNSYVRGGTQQLQTARKLQRNSRKWTC 561
>gb|CF234274.1|CF234274 PtaJXO4E7E0709 Poplar cDNA library from young opposite xylem
Populus alba x Populus tremula cDNA 5', mRNA sequence
Length = 752
Score = 101 bits (215), Expect = 5e-021
Identities = 44/104 (42%), Positives = 65/104 (62%)
Frame = -1 / +1
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I E++ A+ E G+G + + EIQ R DA R++ER LL+L Q+FLDMAV+
Sbjct: 379 IDTLIETGDSEQIFQKAIQEQGRGQITDTLAEIQERHDAVRDLERKLLDLQQIFLDMAVL 558
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
V+ QG+ LD+I S V+NA +V G K +QR+S K +C
Sbjct: 559 VDAQGDMLDNIXSQVSNAVXHVXSGXCCSYKGXXFQRNSXKWMC 690
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 222,081,004
Number of Sequences: 369679
Number of extensions: 2602529
Number of successful extensions: 31500
Number of sequences better than 1.0e-020: 14
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 8174526253
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)