TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521459.2.2
(850 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV274873.1|CV274873 WS0174.B21.1_K13 PTxD-NR-A-8 Populus... 194 7e-049
gb|DT520673.1|DT520673 WS02448.B21_D13 PTxD-ICC-N-A-14 Popu... 194 7e-049
gb|CN523137.1|CN523137 GQ015M01.B3_L24 GQ015 Populus tricho... 190 1e-047
gb|CN520070.1|CN520070 GQ0107.B3_A09 GQ010 Populus trichoca... 188 6e-047
gb|CV257819.1|CV257819 WS0247.B21_L22 PTxD-ICC-N-A-14 Popul... 122 1e-036
gb|BU836172.1|BU836172 T083E02 Populus apical shoot cDNA li... 150 8e-036
gb|DN492268.1|DN492268 V059F02.3pR Populus male catkins cDN... 148 4e-035
gb|BU879394.1|BU879394 V059F02 Populus flower cDNA library ... 140 9e-033
gb|CX176039.1|CX176039 A12_69-61_02.ab1 leaf inoculated wit... 111 3e-030
gb|CV228042.1|CV228042 WS0168.B21_O22 PT-DX-A-7 Populus tri... 116 3e-025
gb|CN549936.1|CN549936 GQ0243.B3_L22 GQ024 Populus trichoca... 114 9e-025
gb|BU830435.1|BU830435 T008C07 Populus apical shoot cDNA li... 102 4e-021
>gb|CV274873.1|CV274873 WS0174.B21.1_K13 PTxD-NR-A-8 Populus trichocarpa x Populus
deltoides cDNA clone WS0174_K13 3', mRNA sequence
Length = 885
Score = 194 bits (418), Expect = 7e-049
Identities = 88/161 (54%), Positives = 104/161 (64%)
Frame = -1 / -1
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYG 632
Y HP A SINI W MYF +P DGN GS+A+ CLQ S+RI YG
Sbjct: 780 YPQVLHPIADSININKTVWDMYFRELIPRLVKEGDDGNCGSTAVCDTICLQALSKRIHYG 601
Query: 631 KFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAK 452
+F AKF+ SP+ Y AI AQD +LM +LT VE AV+ RVE KAK FGQEV + +
Sbjct: 600 EFVAEAKFRASPDDYEAAIRAQDSKRLMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGE 421
Query: 451 DKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
G+ PVYKIRP+LVA+LY IMPLTKEV+V YLLRRLD*
Sbjct: 420 IDGTDPVYKIRPTLVADLYGEWIMPLTKEVQVQYLLRRLD* 298
Score = 126 bits (270), Expect = 2e-028
Identities = 70/160 (43%), Positives = 82/160 (51%)
Frame = +3 / +2
Query: 333 SSLLSKYATSTSLVSGMIL*LYSSATKXXXXXXXXXXXXXXAPMFTSCPKILALASTLCS 512
S+LLSKY T TS VSGMI Y SAT+ + TSCPK LA STL
Sbjct: 302 SNLLSKY*T*TSFVSGMIHSPYKSATRVGRILYTGSVPSISPSIVTSCPKALAFISTLFF 481
Query: 513 TARSTVXYVRRFMS*SRSWAIMAGV*ASGDS*NLAXXXNFPXXILLXXXCKQVXSQRALD 692
TA ST YVR +S S A+MA *+SG++ NLA N P IL C+Q+ SQ A+D
Sbjct: 482 TASSTAGYVRISISLLLSCALMAAS*SSGEARNLASATNSP*WILFERACRQIVSQTAVD 661
Query: 693 PALPSLXXXXXXGRXXSKYIFQXXXXILIXSAXGCXXXXY 812
P LPS G KY+ I I SA GC Y
Sbjct: 662 PQLPSSPSLTNLGISSLKYMSHTVLFIFIESAMGCSTCGY 781
>gb|DT520673.1|DT520673 WS02448.B21_D13 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02448_D13 3', mRNA sequence
Length = 897
Score = 194 bits (418), Expect = 7e-049
Identities = 88/161 (54%), Positives = 104/161 (64%)
Frame = -1 / -1
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYG 632
Y HP A SINI W MYF +P DGN GS+A+ CLQ S+RI YG
Sbjct: 699 YPQVLHPIADSININKTVWDMYFRELIPRLVKEGDDGNCGSTAVCDTICLQALSKRIHYG 520
Query: 631 KFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAK 452
+F AKF+ SP+ Y AI AQD +LM +LT VE AV+ RVE KAK FGQEV + +
Sbjct: 519 EFVAEAKFRASPDDYEAAIRAQDSKRLMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGE 340
Query: 451 DKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
G+ PVYKIRP+LVA+LY IMPLTKEV+V YLLRRLD*
Sbjct: 339 IDGTDPVYKIRPTLVADLYGEWIMPLTKEVQVQYLLRRLD* 217
Score = 126 bits (270), Expect = 2e-028
Identities = 70/160 (43%), Positives = 82/160 (51%)
Frame = +3 / +2
Query: 333 SSLLSKYATSTSLVSGMIL*LYSSATKXXXXXXXXXXXXXXAPMFTSCPKILALASTLCS 512
S+LLSKY T TS VSGMI Y SAT+ + TSCPK LA STL
Sbjct: 221 SNLLSKY*T*TSFVSGMIHSPYKSATRVGRILYTGSVPSISPSIVTSCPKALAFISTLFF 400
Query: 513 TARSTVXYVRRFMS*SRSWAIMAGV*ASGDS*NLAXXXNFPXXILLXXXCKQVXSQRALD 692
TA ST YVR +S S A+MA *+SG++ NLA N P IL C+Q+ SQ A+D
Sbjct: 401 TASSTAGYVRISISLLLSCALMAAS*SSGEARNLASATNSP*WILFERACRQIVSQTAVD 580
Query: 693 PALPSLXXXXXXGRXXSKYIFQXXXXILIXSAXGCXXXXY 812
P LPS G KY+ I I SA GC Y
Sbjct: 581 PQLPSSPSLTNLGISSLKYMSHTVLFIFIESAMGCSTCGY 700
>gb|CN523137.1|CN523137 GQ015M01.B3_L24 GQ015 Populus trichocarpa x Populus deltoides cDNA
clone GQ015M01_L24 5', mRNA sequence
Length = 785
Score = 190 bits (409), Expect = 1e-047
Identities = 86/161 (53%), Positives = 102/161 (63%)
Frame = -1 / +1
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYG 632
Y HP SINI W MYF +P DGN GS+A+ CLQ S+RI YG
Sbjct: 190 YPQVLHPITDSININKTVWDMYFRELIPRLVKEGDDGNCGSTAVCDTICLQALSKRIHYG 369
Query: 631 KFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAK 452
+F AKF+ SP+ Y AI AQD +LM +LT VE AV+ RVE KAK FGQEV + +
Sbjct: 370 EFVAEAKFRASPDDYEAAIRAQDSKRLMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGE 549
Query: 451 DKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
G+ PVYKIRP+LVA+LY IMPLTKEV+V YLLRR D*
Sbjct: 550 IDGTDPVYKIRPTLVADLYGEWIMPLTKEVQVQYLLRRXD* 672
Score = 126 bits (269), Expect = 2e-028
Identities = 69/160 (43%), Positives = 80/160 (50%)
Frame = +3 / -1
Query: 333 SSLLSKYATSTSLVSGMIL*LYSSATKXXXXXXXXXXXXXXAPMFTSCPKILALASTLCS 512
S LLSKY T TS VSGMI Y SAT+ + TSCPK LA STL
Sbjct: 668 SXLLSKY*TXTSFVSGMIHSPYKSATRVGRILYTGSVPSISPSIVTSCPKALAFISTLFF 489
Query: 513 TARSTVXYVRRFMS*SRSWAIMAGV*ASGDS*NLAXXXNFPXXILLXXXCKQVXSQRALD 692
TA ST YVR +S S A+MA *+SG++ NLA N P IL C+Q+ SQ A+D
Sbjct: 488 TASSTAGYVRISISLLLSCALMAAS*SSGEARNLASATNSP*WILFERACRQIVSQTAVD 309
Query: 693 PALPSLXXXXXXGRXXSKYIFQXXXXILIXSAXGCXXXXY 812
P LPS G KY+ I I S GC Y
Sbjct: 308 PQLPSSPSLTNLGISSLKYMSHTVLFIFIESVMGCSTCGY 189
>gb|CN520070.1|CN520070 GQ0107.B3_A09 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0107_A09 5', mRNA sequence
Length = 698
Score = 188 bits (404), Expect = 6e-047
Identities = 86/156 (55%), Positives = 98/156 (62%)
Frame = -1 / +2
Query: 796 HPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYGKFXXX 617
HP A SINI W MYF LP D N GS+A+ CLQ SRRI YGKF
Sbjct: 122 HPCADSININKKVWNMYFTDLLPKLAKAGDDDNCGSAAVCDTVCLQALSRRIHYGKFVAE 301
Query: 616 AKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKDKGSP 437
AKFQES Y AI QDR +LM LLT TVE AV+ RVE K + +GQE I ++ +
Sbjct: 302 AKFQESTAEYEAAIKVQDRARLMELLTYETVEAAVKKRVEMKTRKYGQEGRITQQEDAAD 481
Query: 436 PVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
P+YK+ P LVA+LY IMPLTKEVEV YLLRRLD*
Sbjct: 482 PIYKVEPHLVAQLYEDWIMPLTKEVEVEYLLRRLD* 589
Score = 119 bits (254), Expect = 3e-026
Identities = 69/155 (44%), Positives = 78/155 (50%)
Frame = +3 / -3
Query: 333 SSLLSKYATSTSLVSGMIL*LYSSATKXXXXXXXXXXXXXXAPMFTSCPKILALASTLCS 512
SSLL+KY+TSTS V G+I S ATK + S P L STL
Sbjct: 585 SSLLNKYSTSTSFVRGIIQSS*SWATKCGSTLYMGSAASSCWVILPSWPYFLVFISTLFL 406
Query: 513 TARSTVXYVRRFMS*SRSWAIMAGV*ASGDS*NLAXXXNFPXXILLXXXCKQVXSQRALD 692
TA STV YV MS +RS ++A ++ DS*NLA NFP IL CKQ SQ A D
Sbjct: 405 TAASTVSYVNNSMSLARSCTLIAASYSAVDS*NLASATNFP**ILRDRACKQTVSQTAAD 226
Query: 693 PALPSLXXXXXXGRXXSKYIFQXXXXILIXSAXGC 797
P L S GR KYIF ILI SA GC
Sbjct: 225 PQLSSSPALANFGRRSVKYIFHTFLLILIESAQGC 121
>gb|CV257819.1|CV257819 WS0247.B21_L22 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0247_L22 3', mRNA sequence
Length = 752
Score = 122 bits (262), Expect(2) = 1e-036
Identities = 53/116 (45%), Positives = 67/116 (57%)
Frame = -1 / -1
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYG 632
Y H SINI W MY + LP DGN S+A +CLQ SRRI YG
Sbjct: 653 YSQVLHSGGASININKAIWDMYLNQLLPLFVVAGDDGNYASTAASDLSCLQALSRRIHYG 474
Query: 631 KFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVN 464
KF KF+++P+ Y P I A+D D+LM LLT VE+ V+ RVE KA +FGQ+V+
Sbjct: 473 KFVAEIKFRDAPQDYEPPIRAKDADELMKLLTDERVEKMVKKRVEKKAIVFGQDVS 306
Score = 51.0 bits (105), Expect(2) = 1e-036
Identities = 21/35 (60%), Positives = 27/35 (77%)
Frame = -1 / -1
Query: 430 YKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD*V 326
YK+ PS+VA LY ++ LTK VEV YL+RRLD*+
Sbjct: 278 YKVDPSVVARLYDEWVISLTKRVEVEYLIRRLD*I 174
>gb|BU836172.1|BU836172 T083E02 Populus apical shoot cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 583
Score = 150 bits (323), Expect = 8e-036
Identities = 68/120 (56%), Positives = 85/120 (70%)
Frame = -1 / +2
Query: 688 SALXXXTCLQXXSRRIXYGKFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVE 509
+A+ CLQ S+RI YG++ AKF+ SP+ Y AI AQD +LM +LT VE AV+
Sbjct: 32 TAVCDTICLQALSKRIHYGEYVAEAKFRASPDDYEAAIRAQDSKRLMEILTYPAVEEAVK 211
Query: 508 HRVEAKAKIFGQEVNIGAKDKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
RVE KAK FGQEV + + G+ PVYKI+P+LVA+LY IMPLTKEV+V YLLRRLD*
Sbjct: 212 KRVEMKAKAFGQEVTMEGEIDGTDPVYKIKPTLVADLYGEWIMPLTKEVQVQYLLRRLD* 391
>gb|DN492268.1|DN492268 V059F02.3pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V059F02 3', mRNA sequence
Length = 618
Score = 148 bits (318), Expect = 4e-035
Identities = 68/113 (60%), Positives = 81/113 (71%)
Frame = -1 / -1
Query: 667 CLQXXSRRIXYGKFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKA 488
CLQ S+RI YG+F AKF+ SP+ Y AI A D +LM +LT VE AV+ RVE KA
Sbjct: 603 CLQALSKRIHYGEFVAEAKFRASPDDYEAAIRA*DSKRLMEILTYPAVEEAVKKRVEMKA 424
Query: 487 KIFGQEVNIGAKDKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
K FGQEV + + G+ PVYKIRP+LVA+LY IMPLTKEV+V YLLRRLD*
Sbjct: 423 KAFGQEVTMEGEIDGTDPVYKIRPTLVADLYGEWIMPLTKEVQVQYLLRRLD* 265
>gb|BU879394.1|BU879394 V059F02 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 626
Score = 140 bits (301), Expect = 9e-033
Identities = 65/108 (60%), Positives = 78/108 (72%)
Frame = -1 / +3
Query: 652 SRRIXYGKFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQ 473
S+RI YG+F AKF+ SP+ Y AI A D +LM +LT VE AV+ RVE KAK FGQ
Sbjct: 27 SKRIHYGEFVAEAKFRASPDDYEAAIRA*DSKRLMEILTYPAVEEAVKKRVEMKAKAFGQ 206
Query: 472 EVNIGAKDKGSPPVYKIRPSLVAELYSYRIMPLTKEVEVAYLLRRLD* 329
EV + + G+ PVYKIRP+LVA+LY IMPLTKEV+V YLLRRLD*
Sbjct: 207 EVTMEGEIDGTDPVYKIRPTLVADLYGEWIMPLTKEVQVQYLLRRLD* 350
>gb|CX176039.1|CX176039 A12_69-61_02.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 640
Score = 111 bits (238), Expect(2) = 3e-030
Identities = 51/80 (63%), Positives = 60/80 (75%)
Frame = -1 / +1
Query: 568 QDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKDKGSPPVYKIRPSLVAELYSY 389
QD +LM +LT VE AV+ RVE KAK FGQEV + + G+ PVYKIRP+LVA+LY
Sbjct: 244 QDSKRLMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGEIDGTDPVYKIRPTLVADLYGE 423
Query: 388 RIMPLTKEVEVAYLLRRLD* 329
IMPLTKEV+V YLLRRLD*
Sbjct: 424 WIMPLTKEVQVQYLLRRLD* 483
Score = 40.0 bits (81), Expect(2) = 3e-030
Identities = 17/32 (53%), Positives = 21/32 (65%)
Frame = -1 / +3
Query: 661 QXXSRRIXYGKFXXXAKFQESPEAYTPAIIAQ 566
Q S+RI YG+F AKF+ SP+ Y AI AQ
Sbjct: 54 QALSKRIHYGEFVAEAKFRASPDDYEAAIRAQ 149
>gb|CV228042.1|CV228042 WS0168.B21_O22 PT-DX-A-7 Populus trichocarpa cDNA clone WS0168_O22
3', mRNA sequence
Length = 541
Score = 116 bits (247), Expect = 3e-025
Identities = 54/84 (64%), Positives = 63/84 (75%)
Frame = -1 / +1
Query: 580 AIIAQDRDQLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKDKGSPPVYKIRPSLVAE 401
AI AQD +LM +LT VE AV+ RVE KAK FGQEV + + G+ PVYKIRP+LVA+
Sbjct: 1 AIRAQDSKRLMEILTYPAVEEAVKKRVEMKAKAFGQEVTMEGEIDGTDPVYKIRPTLVAD 180
Query: 400 LYSYRIMPLTKEVEVAYLLRRLD* 329
LY IMPLTKEV+V YLLRRLD*
Sbjct: 181 LYGEWIMPLTKEVQVQYLLRRLD* 252
>gb|CN549936.1|CN549936 GQ0243.B3_L22 GQ024 Populus trichocarpa x Populus deltoides cDNA
clone GQ0243_L22 5', mRNA sequence
Length = 668
Score = 114 bits (243), Expect = 9e-025
Identities = 56/107 (52%), Positives = 58/107 (54%)
Frame = -1 / +3
Query: 811 YXXXXHPXAXSINIXXXXWKMYFDXXLPXXXXXXSDGNAGSSALXXXTCLQXXSRRIXYG 632
Y HP A SINI W MYF LP D N GS+A+ CLQ SRRI YG
Sbjct: 348 YPKVLHPCADSININKKVWNMYFTDLLPKLAKAGDDDNCGSAAVCDTVCLQALSRRIHYG 527
Query: 631 KFXXXAKFQESPEAYTPAIIAQDRDQLMNLLTXXTVERAVEHRVEAK 491
KF AKFQES Y A QDR LM LLT VE AV RVE K
Sbjct: 528 KFVAEAKFQESTAEYEAAXXXQDRAXLMELLTXXXVEAAVXXRVEMK 668
>gb|BU830435.1|BU830435 T008C07 Populus apical shoot cDNA library Populus tremula x Populus
tremuloides cDNA 5 prime, mRNA sequence
Length = 519
Score = 102 bits (217), Expect = 4e-021
Identities = 47/76 (61%), Positives = 57/76 (75%)
Frame = -1 / +2
Query: 556 QLMNLLTXXTVERAVEHRVEAKAKIFGQEVNIGAKDKGSPPVYKIRPSLVAELYSYRIMP 377
+LM +LT VE AV+ VE KAK FGQEV + + G+ PVYKI+P+LVA+LY IMP
Sbjct: 8 RLMEILTYPAVEEAVKK*VEMKAKAFGQEVTMEGEIDGTDPVYKIKPTLVADLYGEWIMP 187
Query: 376 LTKEVEVAYLLRRLD* 329
LTKEV+V YLLRRLD*
Sbjct: 188 LTKEVQVQYLLRRLD* 235
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 376,974,362
Number of Sequences: 369679
Number of extensions: 5708177
Number of successful extensions: 35218
Number of sequences better than 1.0e-020: 15
length of database: 67,802,888
effective HSP length: 52
effective length of database: 48,579,580
effective search space used: 11173303400
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)