TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419405.2.4
(582 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX659519.1|CX659519 PO01031F07 Poplar SC cDNA library Po... 58 5e-025
gb|DT518564.1|DT518564 WS02438.B21_I18 PTxD-ICC-N-A-14 Popu... 56 2e-023
gb|CV260564.1|CV260564 WS02014.B21_G14 PTxN-IB-N-A-11 Popul... 56 2e-023
gb|DT512363.1|DT512363 WS02419.B21_B06 PTxD-ICC-N-A-14 Popu... 56 2e-023
gb|DT517819.1|DT517819 WS02435.B21_G10 PTxD-ICC-N-A-14 Popu... 56 2e-023
gb|CV257933.1|CV257933 WS0248.B21_A20 PTxD-ICC-N-A-14 Popul... 56 2e-023
gb|CV258295.1|CV258295 WS0249.B21_B24 PTxD-ICC-N-A-14 Popul... 56 2e-023
gb|CV259723.1|CV259723 WS02012.B21_B17 PTxN-IB-N-A-11 Popul... 56 2e-023
gb|CV259500.1|CV259500 WS02011.B21_I10 PTxN-IB-N-A-11 Popul... 56 2e-023
>gb|CX659519.1|CX659519 PO01031F07 Poplar SC cDNA library Populus alba x Populus tremula
var. glandulosa cDNA clone PO01031F07 5', mRNA sequence
Length = 633
Score = 57.9 bits (120), Expect(3) = 5e-025
Identities = 18/34 (52%), Positives = 28/34 (82%)
Frame = -2 / +3
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
NS SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 324 NSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 425
Score = 48.7 bits (100), Expect(3) = 5e-025
Identities = 18/30 (60%), Positives = 23/30 (76%)
Frame = -2 / +3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A LVE+EDGDGD+ V+L +A
Sbjct: 231 DSGSNPYYFATLVEYEDGDGDLKSVELKQA 320
Score = 45.1 bits (92), Expect(3) = 5e-025
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / +3
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 429 ESGKTIVASGVIPAGWAPGQTYRSLVNF 512
>gb|DT518564.1|DT518564 WS02438.B21_I18 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02438_I18 3', mRNA sequence
Length = 905
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -1
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 350 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 249
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 443 DSGSNPYYFATVVEYEDGDGELKSVELKQA 354
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -1
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 245 ESGKTIVASGVIPAGWAPGQTYRSLVNF 162
>gb|CV260564.1|CV260564 WS02014.B21_G14 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02014_G14 3', mRNA sequence
Length = 893
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -3
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 402 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 301
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 495 DSGSNPYYFATVVEYEDGDGELKSVELKQA 406
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -3
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 297 ESGKTIVASGVIPAGWAPGQTYRSLVNF 214
>gb|DT512363.1|DT512363 WS02419.B21_B06 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02419_B06 3', mRNA sequence
Length = 837
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -2
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 350 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 249
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 443 DSGSNPYYFATVVEYEDGDGELKSVELKQA 354
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -2
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 245 ESGKTIVASGVIPAGWAPGQTYRSLVNF 162
>gb|DT517819.1|DT517819 WS02435.B21_G10 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS02435_G10 3', mRNA sequence
Length = 831
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -1
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 330 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 229
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 423 DSGSNPYYFATVVEYEDGDGELKSVELKQA 334
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -1
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 225 ESGKTIVASGVIPAGWAPGQTYRSLVNF 142
>gb|CV257933.1|CV257933 WS0248.B21_A20 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0248_A20 3', mRNA sequence
Length = 808
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -3
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 329 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 228
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 422 DSGSNPYYFATVVEYEDGDGELKSVELKQA 333
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -3
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 224 ESGKTIVASGVIPAGWAPGQTYRSLVNF 141
>gb|CV258295.1|CV258295 WS0249.B21_B24 PTxD-ICC-N-A-14 Populus trichocarpa x Populus
deltoides cDNA clone WS0249_B24 3', mRNA sequence
Length = 741
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -1
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 426 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 325
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 519 DSGSNPYYFATVVEYEDGDGELKSVELKQA 430
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -1
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 321 ESGKTIVASGVIPAGWAPGQTYRSLVNF 238
>gb|CV259723.1|CV259723 WS02012.B21_B17 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02012_B17 3', mRNA sequence
Length = 724
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -2
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 354 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 253
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 447 DSGSNPYYFATVVEYEDGDGELKSVELKQA 358
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -2
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 249 ESGKTIVASGVIPAGWAPGQTYRSLVNF 166
>gb|CV259500.1|CV259500 WS02011.B21_I10 PTxN-IB-N-A-11 Populus trichocarpa x Populus nigra
cDNA clone WS02011_I10 3', mRNA sequence
Length = 458
Score = 55.6 bits (115), Expect(3) = 2e-023
Identities = 17/34 (50%), Positives = 28/34 (82%)
Frame = -2 / -1
Query: 491 NSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITN 390
+S SW PM++SWG++W+LD+G L AP S+++T+
Sbjct: 350 DSDSWVPMQKSWGAVWKLDAGSLLRAPLSIKLTS 249
Score = 45.5 bits (93), Expect(3) = 2e-023
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -2 / -1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP Y A +VE+EDGDG++ V+L +A
Sbjct: 443 DSGSNPYYFATVVEYEDGDGELKSVELKQA 354
Score = 45.1 bits (92), Expect(3) = 2e-023
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = -2 / -1
Query: 389 ESGKTLVANQVIPANWVPNTYYRSIIQY 306
ESGKT+VA+ VIPA W P YRS++ +
Sbjct: 245 ESGKTIVASGVIPAGWAPGQTYRSLVNF 162
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 258,962,416
Number of Sequences: 369679
Number of extensions: 3759280
Number of successful extensions: 71838
Number of sequences better than 1.0e-020: 9
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 6950794778
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)