TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161254.2.1
(1192 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, comple... 130 1e-097
gb|DT500038.1|DT500038 PX0011.BR_A12 PT-X-FL-A-1 Populus tr... 274 8e-074
gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus t... 169 5e-051
gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDN... 162 3e-039
gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-... 145 6e-034
>gb|AC149484.1| Populus trichocarpa clone Pop1-69M19, complete sequence
Length = 96930
Score = 130 bits (278), Expect(8) = 1e-097
Identities = 44/48 (91%), Positives = 47/48 (97%)
Frame = +1 / -2
Query: 1048 GGCFVEGDWLRNAFRWRESIGPWEERPGHFGDVWHYWTDDGLGYYEFL 1191
GGCFVEG+WLRNAFRW+ESIGPWEERPGHFGDVW YWTDDGLGY+EFL
Sbjct: 70706 GGCFVEGEWLRNAFRWKESIGPWEERPGHFGDVWMYWTDDGLGYFEFL 70563
Score = 95.0 bits (201), Expect(8) = 1e-097
Identities = 38/49 (77%), Positives = 42/49 (85%)
Frame = +1 / -1
Query: 511 GFEAGGSHTPSNIDPWSIIGDDSSVFVATDRTSCFSRNIVALRMEVLCD 657
GFEAGG +TPSNI PWSIIGD SS+ V+TDR+SCF RN VALRM VLCD
Sbjct: 72342 GFEAGGQNTPSNIAPWSIIGDQSSLTVSTDRSSCFERNKVALRMHVLCD 72196
Score = 80.3 bits (169), Expect(6) = 2e-041
Identities = 35/48 (72%), Positives = 37/48 (77%)
Frame = -3 / +1
Query: 1190 RNS*YPSPSSVQ*CQTSPKCPGLSSHGPIDSLHLNAFLSQSPSTKQPP 1047
RNS PSPSSVQ QTSPKCPGLSS GP+ S NAFL+ SPSTK PP
Sbjct: 70564 RNSK*PSPSSVQYIQTSPKCPGLSSQGPMLSFQRNAFLNHSPSTKHPP 70707
Score = 80.3 bits (169), Expect(8) = 1e-097
Identities = 32/48 (66%), Positives = 37/48 (77%)
Frame = +1 / -3
Query: 835 SGTSNWTKVEQKLVAKGTNRTSRLQITTNKKGVVWLDQVSLMPEDTYK 978
S SNWTK E L AKGTN SRLQ+TT++KGV+W DQVS MP +TYK
Sbjct: 71812 SDVSNWTKTEVLLEAKGTNPNSRLQLTTSRKGVIWFDQVSAMPLETYK 71669
Score = 60.2 bits (125), Expect(6) = 2e-041
Identities = 28/50 (56%), Positives = 38/50 (76%)
Frame = -3 / +3
Query: 977 LYVSSGMSDT*SSQTTPFLFVVI*SLDVLLVPLATSFCSTFVQFDVPETV 828
LYVS G++DT*S+ TPFL VV *SL++ VPLA++ S FVQF+ E++
Sbjct: 71670 LYVSKGIADT*SNHITPFLDVVN*SLELGFVPLASNRTSVFVQFETSESL 71819
Score = 56.1 bits (116), Expect(6) = 2e-041
Identities = 25/49 (51%), Positives = 34/49 (69%)
Frame = -3 / +2
Query: 656 SQRTSILRATMFRLKHDVRSVATNTEESSPMMDHGSMFDGV*EPPASKP 510
SQ T I AT+ R K + RSV T ++ SP++D G++F+GV PPASKP
Sbjct: 72197 SQSTCIRSATLLRSKQEERSVDTVKDD*SPIIDQGAIFEGVFWPPASKP 72343
Score = 53.8 bits (111), Expect(8) = 1e-097
Identities = 24/40 (60%), Positives = 32/40 (80%)
Frame = +1 / -3
Query: 709 NIEDGKAYNLVMYVQSPETKDLTVSLTSSDGLQNLASATI 828
NIE GK Y +V+YV+S + +++VSLTSSDGLQ LA+A I
Sbjct: 72022 NIEQGKIYKVVLYVRSLGSINVSVSLTSSDGLQILATANI 71903
Score = 53.3 bits (110), Expect(8) = 1e-097
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +1 / -3
Query: 448 FFEEINHAGAGGIWAELVSNRG 513
F +EINHAGAGGIWAELVSNRG
Sbjct: 72742 FLQEINHAGAGGIWAELVSNRG 72677
Score = 48.3 bits (99), Expect(8) = 1e-097
Identities = 17/25 (68%), Positives = 22/25 (88%)
Frame = +1 / -1
Query: 976 KGHGFRTELISMLLDLKPRFLRFPG 1050
+GHGF+ ELI ML D+KP+F+RFPG
Sbjct: 71367 QGHGFQKELIEMLEDIKPQFIRFPG 71293
Score = 48.3 bits (99), Expect(8) = 1e-097
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +1 / -1
Query: 661 CPAGGVGVYNPGFWGM 708
CPAGGVG+YNPGFWGM
Sbjct: 72177 CPAGGVGIYNPGFWGM 72130
Score = 43.2 bits (88), Expect(6) = 2e-041
Identities = 16/21 (76%), Positives = 18/21 (85%)
Frame = -3 / +3
Query: 512 PLLLTSSAHMPPAPAWLISSK 450
P LLTSSAH+PPAPAW IS +
Sbjct: 72678 PRLLTSSAHIPPAPAWFISCR 72740
Score = 34.1 bits (68), Expect(6) = 2e-041
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = -2 / +1
Query: 708 HAPKTWVINTNTTSWTLIT 652
HAPK W+I +N+ SW IT
Sbjct: 72130 HAPKPWIIYSNSASWANIT 72186
Score = 32.2 bits (64), Expect(8) = 1e-097
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = +1 / -2
Query: 358 SEIEATQTAALKVDASPQLARKIPETLFGIFFE 456
S E K+ + R IPETLFGIFFE
Sbjct: 72962 SAAEVDAYQTAKLIVNASSGRPIPETLFGIFFE 72864
Score = 28.1 bits (55), Expect(6) = 2e-041
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -3 / +3
Query: 827 IVAEAKFCNPSELVNET 777
++A A C+PSELVN+T
Sbjct: 71904 MLAVANICSPSELVNDT 71954
>gb|DT500038.1|DT500038 PX0011.BR_A12 PT-X-FL-A-1 Populus trichocarpa cDNA clone PX0011_A12
5', mRNA sequence
Length = 513
Score = 274 bits (592), Expect(2) = 8e-074
Identities = 108/150 (72%), Positives = 126/150 (84%)
Frame = +1 / +2
Query: 661 CPAGGVGVYNPGFWGMNIEDGKAYNLVMYVQSPETKDLTVSLTSSDGLQNLASATITVSG 840
CPAGGVG+YNPGFWGMNIE GKAY +V+YV+S + +++VSLTSSDG Q LASA I S
Sbjct: 62 CPAGGVGIYNPGFWGMNIEQGKAYKVVLYVRSLGSINVSVSLTSSDGSQILASANIVDSD 241
Query: 841 TSNWTKVEQKLVAKGTNRTSRLQITTNKKGVVWLDQVSLMPEDTYKGHGFRTELISMLLD 1020
SNWTK E L AKGTN SRLQ+TT++KGV+W DQVS MP DTYKGHGFR ELI M+ D
Sbjct: 242 VSNWTKAEVLLEAKGTNPNSRLQLTTSRKGVIWFDQVSAMPLDTYKGHGFRNELIEMIDD 421
Query: 1021 LKPRFLRFPGGCFVEGDWLRNAFRWRESIG 1110
+KP+F+RFPGGCFVEG+WLRNAFRW+ESIG
Sbjct: 422 IKPQFVRFPGGCFVEGEWLRNAFRWKESIG 511
Score = 193 bits (416), Expect = 2e-048
Identities = 88/150 (58%), Positives = 108/150 (72%)
Frame = -3 / -1
Query: 1112 GPIDSLHLNAFLSQSPSTKQPPGNLKNRGFKSKSMDISSVRKPCPLYVSSGMSDT*SSQT 933
GP+ S NAFL+ SPSTKQPPGNL N GF S + +SS RKPCPLYVS G++DT*S+
Sbjct: 513 GPMLSFQRNAFLNHSPSTKQPPGNLTNWGFISSIISMSSFRKPCPLYVSRGIADT*SNHI 334
Query: 932 TPFLFVVI*SLDVLLVPLATSFCSTFVQFDVPETVIVAEAKFCNPSELVNETVRSFVSGD 753
TPFL VV *SL++ VPLA++ S FVQF+ E+ ++A+A C+PSELVN+T +
Sbjct: 333 TPFLDVVN*SLELGFVPLASNRTSAFVQFETSESTMLADANICDPSELVNDTETLIDPRE 154
Query: 752 *TYITRL*AFPSSMFMPQKPGL*TPTPPAG 663
T T L*AFP S+FMPQ PGL PTPPAG
Sbjct: 153 RT*RTTL*AFPCSIFMPQNPGLYIPTPPAG 64
Score = 81.7 bits (172), Expect(2) = 1e-025
Identities = 46/105 (43%), Positives = 58/105 (55%)
Frame = -2 / -3
Query: 1110 TN*FSPSECIS*PVTFYKTTSRKSQESWFQIQKHGYKFCAETMSLVCVFRHE*YLIKPNN 931
+N F P++C+S P T K + KS + F I H + +ETM LVCV RH *+LIKP
Sbjct: 511 SNAFLPAKCVSQPFTLNKAATWKSDKLGFYIIDHFNELISETMPLVCVQRHS*HLIKPYY 332
Query: 930 SFFVRCYLKP*CSIGSFSNQLLLHFCPI*CARNCYCS*SQVLQPI 796
+F LKP* I SF Q L CP+* R S Q L+PI
Sbjct: 331 TFSGCSQLKP*IRICSFGFQ*NLSLCPV*NIRIYNVSRCQYLRPI 197
Score = 55.6 bits (115), Expect(2) = 1e-025
Identities = 18/33 (54%), Positives = 25/33 (75%)
Frame = -2 / -3
Query: 750 NIHNEIVGLPIFYVHAPKTWVINTNTTSWTLIT 652
+I N +GLP+F +HAPK W++ TN+TSW IT
Sbjct: 151 DIKNHFIGLPLFNIHAPKPWIVYTNSTSWANIT 53
Score = 24.4 bits (47), Expect(2) = 8e-074
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +1 / +2
Query: 628 VALRMEVLCD 657
VALRM+VLCD
Sbjct: 14 VALRMDVLCD 43
>gb|DT474803.1|DT474803 WS0125.BR_A19 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS0125_A19 5', mRNA sequence
Length = 585
Score = 169 bits (364), Expect(2) = 5e-051
Identities = 70/103 (67%), Positives = 79/103 (76%)
Frame = +1 / +3
Query: 349 CRPSEIEATQTAALKVDASPQLARKIPETLFGIFFEEINHAGAGGIWAELVSNRGFEAGG 528
C+ S E + + R IPETLFGIFFEEINHAGAGGIWAELV+NRGFEAGG
Sbjct: 204 CQCSAAEVDANQTANLIINGSSTRPIPETLFGIFFEEINHAGAGGIWAELVNNRGFEAGG 383
Query: 529 SHTPSNIDPWSIIGDDSSVFVATDRTSCFSRNIVALRMEVLCD 657
+TPSNI PWSIIGD SS+ V+TDR+SCF RN VALRM+VLCD
Sbjct: 384 QNTPSNIAPWSIIGDQSSLIVSTDRSSCFERNKVALRMDVLCD 512
Score = 120 bits (257), Expect(2) = 2e-032
Identities = 53/80 (66%), Positives = 62/80 (77%)
Frame = -3 / -3
Query: 656 SQRTSILRATMFRLKHDVRSVATNTEESSPMMDHGSMFDGV*EPPASKPLLLTSSAHMPP 477
SQ TSI RAT+FR K + RSV T ++ SPM+D G++F+GV PPASKP L TSSAH+PP
Sbjct: 511 SQSTSIRRATLFRSKQEERSVDTIKDD*SPMIDQGAIFEGVFWPPASKPRLFTSSAHIPP 332
Query: 476 APAWLISSKNIPNSVSGIFR 417
APAW ISSK IP SVSGI R
Sbjct: 331 APAWFISSKKIPKSVSGIGR 272
Score = 73.0 bits (153), Expect(3) = 8e-021
Identities = 32/80 (40%), Positives = 48/80 (60%)
Frame = +3 / +2
Query: 417 PEDP*NTVWDIF*GDQPCRSWWHMGRACQ*QRFRSWRLSYSIKHRPVVHHWR*LLRVCGD 596
P *NT+W+ GD+PC SWW MGRAC+ F S +S+K+ +V+HW ++ C
Sbjct: 272 PAYS*NTLWNFLRGDKPCWSWWDMGRACKQSGF*SRGPKHSLKYCSLVYHW*SVIFNCIY 451
Query: 597 *SYIMFQSKHCCSEDGGPL* 656
* + + ++K CS +G L*
Sbjct: 452 *PFFLLRAKQGCSPNGCAL* 511
Score = 52.8 bits (109), Expect(2) = 5e-051
Identities = 17/18 (94%), Positives = 18/18 (100%)
Frame = +1 / +3
Query: 661 CPAGGVGVYNPGFWGMNI 714
CPAGGVG+YNPGFWGMNI
Sbjct: 531 CPAGGVGIYNPGFWGMNI 584
Score = 39.1 bits (79), Expect(2) = 2e-032
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = -2 / -2
Query: 711 VHAPKTWVINTNTTSWTLIT 652
+HAPK W++ TN+TSW IT
Sbjct: 581 IHAPKPWIVYTNSTSWANIT 522
Score = 35.0 bits (70), Expect(3) = 8e-021
Identities = 14/44 (31%), Positives = 24/44 (54%)
Frame = +1 / +3
Query: 274 KPMDLKQASSGTIFCLLLLFCVGCRCRPSEIEATQTAALKVDAS 405
K K + S + C ++ C C+C +E++A QTA L ++ S
Sbjct: 135 KMSSCKASCSVLLACFIVGLCSVCQCSAAEVDANQTANLIINGS 266
Score = 30.4 bits (60), Expect(3) = 8e-021
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +3 / +2
Query: 666 SWWCWCL*PRFLGHEH 713
SWW W + R LGHE+
Sbjct: 536 SWWSWYIQSRVLGHEY 583
>gb|DN501581.1|DN501581 V006H08.5pR Populus male catkins cDNA library Populus trichocarpa
cDNA clone V006H08 5', mRNA sequence
Length = 508
Score = 162 bits (349), Expect = 3e-039
Identities = 66/102 (64%), Positives = 77/102 (75%)
Frame = +1 / +2
Query: 349 CRPSEIEATQTAALKVDASPQLARKIPETLFGIFFEEINHAGAGGIWAELVSNRGFEAGG 528
C+ S E + + R IPETLFGIFFEEINHAGAGGIWAELV+NRGFE+GG
Sbjct: 203 CQCSAAEVDANQTANLIINGSSTRPIPETLFGIFFEEINHAGAGGIWAELVNNRGFESGG 382
Query: 529 SHTPSNIDPWSIIGDDSSVFVATDRTSCFSRNIVALRMEVLC 654
+TPSNI PWS+IGD SS+ V+TDR+SCF RN ALRM+VLC
Sbjct: 383 QNTPSNIAPWSLIGDQSSLIVSTDRSSCFERNNAALRMDVLC 508
Score = 112 bits (240), Expect = 3e-024
Identities = 50/79 (63%), Positives = 58/79 (73%)
Frame = -3 / -2
Query: 653 QRTSILRATMFRLKHDVRSVATNTEESSPMMDHGSMFDGV*EPPASKPLLLTSSAHMPPA 474
Q TSI RA +FR K + RSV T ++ SPM D G++F+GV PP SKP L TSSAH+PPA
Sbjct: 507 QSTSIRRAALFRSKQEERSVDTIKDD*SPMRDQGAIFEGVFWPPDSKPRLFTSSAHIPPA 328
Query: 473 PAWLISSKNIPNSVSGIFR 417
PAW ISSK IP SVSGI R
Sbjct: 327 PAWFISSKKIPKSVSGIGR 271
>gb|AJ774822.1|AJ774822 AJ774822 Populus euphratica shoot 3-6 months Populus euphratica
cDNA clone P0000300024F09F1, mRNA sequence
Length = 538
Score = 145 bits (311), Expect = 6e-034
Identities = 60/91 (65%), Positives = 67/91 (73%)
Frame = +1 / +2
Query: 349 CRPSEIEATQTAALKVDASPQLARKIPETLFGIFFEEINHAGAGGIWAELVSNRGFEAGG 528
C S E + + R IPETLFGIFFEEINHAGAGGIWAELV+NRGFEAGG
Sbjct: 266 CPCSAAEVDANQTANLIINGSSTRPIPETLFGIFFEEINHAGAGGIWAELVNNRGFEAGG 445
Query: 529 SHTPSNIDPWSIIGDDSSVFVATDRTSCFSR 621
+TPSNI PWSIIGD SS+ V+TDR+SCF R
Sbjct: 446 QNTPSNIAPWSIIGDQSSLIVSTDRSSCFER 538
Score = 101 bits (216), Expect = 7e-021
Identities = 44/68 (64%), Positives = 52/68 (76%)
Frame = -3 / -2
Query: 620 RLKHDVRSVATNTEESSPMMDHGSMFDGV*EPPASKPLLLTSSAHMPPAPAWLISSKNIP 441
R K + RSV T ++ SPM+D G++F+GV PPASKP L TSSAH+PPAPAW ISSK IP
Sbjct: 537 RSKQEERSVDTIKDD*SPMIDQGAIFEGVFWPPASKPRLFTSSAHIPPAPAWFISSKKIP 358
Query: 440 NSVSGIFR 417
SVSGI R
Sbjct: 357 KSVSGIGR 334
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 649,531,243
Number of Sequences: 369679
Number of extensions: 10509046
Number of successful extensions: 61032
Number of sequences better than 1.0e-020: 5
length of database: 67,802,888
effective HSP length: 53
effective length of database: 48,209,901
effective search space used: 16535996043
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)