TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS19f07.yg.2.1
(626 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciat... 163 1e-039
gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo li... 150 6e-036
gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo li... 150 6e-036
gb|BF518211.1|BF518211 NXSI_036_D07_F NXSI (Nsf Xylem Side ... 74 6e-026
gb|DN612931.1|DN612931 EST965981 Subtracted pine embryo lib... 107 6e-023
gb|DT628876.1|DT628876 EST1155446 Subtracted pine embryo li... 107 6e-023
gb|CF479164.1|CF479164 RTWW3_24_E11.g1_A022 Well-watered lo... 105 3e-022
>gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP032F08, mRNA sequence
Length = 674
Score = 163 bits (350), Expect = 1e-039
Identities = 66/120 (55%), Positives = 79/120 (65%)
Frame = -1 / +1
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S + + AA HA A F I+YNPR P+EFVIP KY K+ S+GMR
Sbjct: 313 SSVLSSHGIHSGVLAAVAHAVATKSMFHIYYNPRTSPTEFVIPYHKYVKSFNHSFSIGMR 492
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIE 261
F+M FETED+ RRY GTI GIGD+DP+RW NS WR+ +VGWDE A R+ RVS WEIE
Sbjct: 493 FKMRFETEDATERRYTGTIVGIGDVDPMRWPNSRWRSFKVGWDEHAAQXRQDRVSPWEIE 672
>gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAB186 3' end, mRNA sequence
Length = 841
Score = 150 bits (323), Expect = 6e-036
Identities = 62/121 (51%), Positives = 75/121 (61%)
Frame = -1 / +2
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S + AA A A F +FYNPR P+EF+IP KY K+ +S+GMR
Sbjct: 68 SSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTSPAEFIIPYQKYVKSCKQPLSIGMR 247
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIE 261
F+M FETED+ R Y G IT IGD DP RW S WR L+V WDE A+E++ RVS WEIE
Sbjct: 248 FKMRFETEDTAERSYTGMITAIGDADPARWPGSKWRLLKVEWDEHAANEQQERVSAWEIE 427
Query: 260 P 258
P
Sbjct: 428 P 430
>gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFN79 3' end, mRNA sequence
Length = 783
Score = 150 bits (323), Expect = 6e-036
Identities = 62/121 (51%), Positives = 75/121 (61%)
Frame = -1 / +2
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S + AA A A F +FYNPR P+EF+IP KY K+ +S+GMR
Sbjct: 68 SSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTSPAEFIIPYQKYVKSCKQPLSIGMR 247
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIE 261
F+M FETED+ R Y G IT IGD DP RW S WR L+V WDE A+E++ RVS WEIE
Sbjct: 248 FKMRFETEDTAERSYTGMITAIGDADPARWPGSKWRLLKVEWDEHAANEQQERVSAWEIE 427
Query: 260 P 258
P
Sbjct: 428 P 430
>gb|BF518211.1|BF518211 NXSI_036_D07_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_036_D07 5' similar to Arabidopsis thaliana
sequence At1g30330 putative protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 323
Score = 73.9 bits (155), Expect(2) = 6e-026
Identities = 32/54 (59%), Positives = 36/54 (66%)
Frame = -1 / +1
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ S S M + AA HAAA + FTIFYNPRA PSEFVIPLAKY KA+Y
Sbjct: 58 MPSLVLSSDSMHIGLLAAAAHAAATNSRFTIFYNPRASPSEFVIPLAKYVKAVY 219
Score = 62.9 bits (131), Expect(2) = 6e-026
Identities = 25/33 (75%), Positives = 29/33 (87%)
Frame = -1 / +1
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGD 369
+T+VS+GMRF MLFETE+ VRRYMGTITGI D
Sbjct: 220 HTRVSIGMRFXMLFETEEXXVRRYMGTITGIXD 318
>gb|DN612931.1|DN612931 EST965981 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PSABL95, mRNA sequence
Length = 596
Score = 107 bits (229), Expect = 6e-023
Identities = 42/99 (42%), Positives = 58/99 (58%)
Frame = -1 / -1
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA F+++Y PR PSEF+IP +Y +++ S+GMR
Sbjct: 299 SSVISSHSMHLGVIATASHAVTTKTMFSVYYKPRTSPSEFIIPYDQYMESMKINFSVGMR 120
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQ 324
F+M FE E+ +R+ GTI GI D DP+ W NS WR L+
Sbjct: 119 FKMKFEGEEVPEQRFTGTIVGISDADPVNWPNSKWRCLK 3
>gb|DT628876.1|DT628876 EST1155446 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PIMD303 5' end, mRNA sequence
Length = 596
Score = 107 bits (229), Expect = 6e-023
Identities = 42/99 (42%), Positives = 58/99 (58%)
Frame = -1 / +1
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA F+++Y PR PSEF+IP +Y +++ S+GMR
Sbjct: 298 SSVISSHSMHLGVIATASHAVTTKTMFSVYYKPRTSPSEFIIPYDQYMESMKINFSVGMR 477
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQ 324
F+M FE E+ +R+ GTI GI D DP+ W NS WR L+
Sbjct: 478 FKMKFEGEEVPEQRFTGTIVGISDADPVNWPNSKWRCLK 594
>gb|CF479164.1|CF479164 RTWW3_24_E11.g1_A022 Well-watered loblolly pine roots WW3 Pinus
taeda cDNA clone RTWW3_24_E11_A022 5', mRNA sequence
Length = 714
Score = 105 bits (224), Expect = 3e-022
Identities = 43/72 (59%), Positives = 51/72 (70%)
Frame = -1 / +2
Query: 404 RRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPP 225
RRYMGTI GI DLD +RW NSHWR+++VGWDESTA ER+ RVS+WEIEP+ T P
Sbjct: 2 RRYMGTIIGISDLDQVRWPNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTTFPMYSSPY 181
Query: 224 FFRPKLPKQAGM 189
R K P G+
Sbjct: 182 PLRLKRPWPQGI 217
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 346,133,939
Number of Sequences: 355925
Number of extensions: 5290421
Number of successful extensions: 60404
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 8520950490
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)