TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN28g01.yg.2.1
(513 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW225867.1|AW225867 ST72F08 Pine TriplEx shoot tip libra... 144 4e-034
>gb|AW225867.1|AW225867 ST72F08 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST72F08, mRNA sequence
Length = 396
Score = 144 bits (309), Expect = 4e-034
Identities = 61/104 (58%), Positives = 75/104 (72%)
Frame = -1 / +2
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
L+ +LT K S VP N +ARRRI+FF+NSLFM+MP A +V QM+ FSVLTPYY EDV++
Sbjct: 83 LQTILTTKNSMHRVPRNFEARRRIAFFSNSLFMNMPRAVQVEQMMSFSVLTPYYDEDVMY 262
Query: 315 SSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVHCESEDQLHE 184
+ + L NEDG+SILFYLQKIYPDE NF ER+ L E
Sbjct: 263 NREQLRTPNEDGISILFYLQKIYPDEXXNFXERMRXXXXXDLDE 394
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 269,329,696
Number of Sequences: 355925
Number of extensions: 3746288
Number of successful extensions: 56195
Number of sequences better than 1.0e-020: 2
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 6458554830
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)