TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN21b10.yg.2.1
(574 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX712891.1|CX712891 RTPQ1_5_B06.g1_A032 Roots treated wi... 300 4e-081
gb|DR117061.1|DR117061 RTMG1_4_B01.g1_A029 Roots minus magn... 244 4e-064
gb|DT632465.1|DT632465 EST1147396 Normalized pine embryo li... 236 1e-061
gb|DT638404.1|DT638404 EST1153335 Normalized pine embryo li... 200 5e-051
gb|DR685171.1|DR685171 EST1075248 Normalized pine embryo li... 128 3e-048
gb|DR116979.1|DR116979 RTMG1_4_B01.b1_A029 Roots minus magn... 175 2e-043
gb|DR694121.1|DR694121 EST1084211 Normalized pine embryo li... 171 5e-042
gb|DR111377.1|DR111377 RTS1_17_F12.b1_A029 Roots minus sulf... 140 8e-033
>gb|CX712891.1|CX712891 RTPQ1_5_B06.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_5_B06_A032 5', mRNA sequence
Length = 781
Score = 300 bits (650), Expect = 4e-081
Identities = 115/170 (67%), Positives = 140/170 (82%)
Frame = +3 / -1
Query: 36 FVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQNLTAANIMKCD 215
FVALN+RKE+N+ LKKEA+++GD+VI+PF+D YELVVLKT+AICEYGV+ L+A +MKCD
Sbjct: 781 FVALNARKEINLQLKKEADFYGDMVIVPFMDNYELVVLKTLAICEYGVRRLSAKYVMKCD 602
Query: 216 DDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDEEWPEDIYPPYANGPG 395
DD FVR+D VL+ + + KPLY+GNLN H+PLR GKWAVT EEWPED YPPYANGPG
Sbjct: 601 DDNFVRIDAVLKEVNRLSHGKPLYIGNLNYFHKPLRVGKWAVTYEEWPEDDYPPYANGPG 422
Query: 396 YVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVEKFNSTXXVXYSHSWK 545
Y+I+ DIA FIVSQ NQ LF MEDVSMG+WVE+FN++ V YSH WK
Sbjct: 421 YIITRDIANFIVSQHKNQTLRLFKMEDVSMGMWVEQFNASTPVQYSHDWK 272
Score = 157 bits (338), Expect = 5e-038
Identities = 84/169 (49%), Positives = 97/169 (57%)
Frame = -1 / +3
Query: 544 FQLXEYXTXXVELNFSTHRPMLTSSIXNSXXL*LARCDTMNFAMSPEIT*PGPFAYXXXX 365
FQ EY T LN STH PMLTSSI N+ +* DT+ FAMS + PGPFA
Sbjct: 273 FQSWEY*TGVDALNCSTHIPMLTSSILNNLRV*FLC*DTIKFAMSLVMIYPGPFA*GG*S 452
Query: 364 XXXXXXSVTAHFPVLNGLCRRLRFPIYNGLSPLFNLMRLRTTSTLTNVSSSHFMMFAAVK 185
VTAHFP L GL + R P+Y G L T S LT +SSSHF+ + A
Sbjct: 453 SSGHSSYVTAHFPTLRGL*K*FRLPMYRGFP*DNLLTSFSTASILTKLSSSHFITYLADN 632
Query: 184 FWTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFFNITLTSFLEFSAT 38
TPYS IA V +TTSS* S+NG +T+SP SASFFN SFL F AT
Sbjct: 633 LRTPYSHIARVFKTTSS*LSMNGTITISP*KSASFFNCKFISFLAFKAT 779
>gb|DR117061.1|DR117061 RTMG1_4_B01.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_4_B01_A029 5', mRNA sequence
Length = 800
Score = 244 bits (527), Expect = 4e-064
Identities = 94/138 (68%), Positives = 110/138 (79%)
Frame = +3 / +3
Query: 132 YELVVLKTIAICEYGVQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLH 311
YELVVLKT+AICEYGV+ L+A +MKCDDD FVR+D VL+ + + KPLY+GNLN H
Sbjct: 21 YELVVLKTLAICEYGVRRLSAKYVMKCDDDNFVRIDAVLKEVNRLSHGKPLYIGNLNYFH 200
Query: 312 RPLRTGKWAVTDEEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGL 491
+PLR GKWAVT EEWPED YPPYANGPGY+I+ DIA FIVSQ NQ LF MEDVSMG+
Sbjct: 201 KPLRVGKWAVTYEEWPEDDYPPYANGPGYIITRDIANFIVSQHKNQTLRLFKMEDVSMGM 380
Query: 492 WVEKFNSTXXVXYSHSWK 545
WVE+FN++ V YSH WK
Sbjct: 381 WVEQFNASTPVQYSHDWK 434
Score = 120 bits (257), Expect = 7e-027
Identities = 66/138 (47%), Positives = 76/138 (55%)
Frame = -1 / -2
Query: 544 FQLXEYXTXXVELNFSTHRPMLTSSIXNSXXL*LARCDTMNFAMSPEIT*PGPFAYXXXX 365
FQ EY T LN STH PMLTSSI N+ +* DT+ FAMS + PGPFA
Sbjct: 433 FQSWEY*TGVDALNCSTHIPMLTSSILNNLRV*FLC*DTIKFAMSLVMIYPGPFA*GG*S 254
Query: 364 XXXXXXSVTAHFPVLNGLCRRLRFPIYNGLSPLFNLMRLRTTSTLTNVSSSHFMMFAAVK 185
VTAHFP L GL + R P+Y G L T S LT +SSSHF+ + A
Sbjct: 253 SSGHSSYVTAHFPTLRGL*K*FRLPMYRGFP*DNLLTSFSTASILTKLSSSHFITYLADN 74
Query: 184 FWTPYSQIAIVLRTTSS* 131
TPYS IA V +TTSS*
Sbjct: 73 LRTPYSHIARVFKTTSS* 20
>gb|DT632465.1|DT632465 EST1147396 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFK30 3' end, mRNA sequence
Length = 856
Score = 236 bits (509), Expect = 1e-061
Identities = 91/129 (70%), Positives = 109/129 (84%)
Frame = +3 / +3
Query: 45 LNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQNLTAANIMKCDDDT 224
+N+RKEVNV LK EA+++GDI+I+PF+DRYELVVLKT+AICEYGV+N++A +MKCDDDT
Sbjct: 468 MNARKEVNVQLKNEADFYGDIIIVPFMDRYELVVLKTVAICEYGVRNISAKYVMKCDDDT 647
Query: 225 FVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDEEWPEDIYPPYANGPGYVI 404
FVRVD VL+ IK LYMGNLNL HRPLR GKWAVT EEWPE+ YP YANGPGY+I
Sbjct: 648 FVRVDTVLKEIKRTPHVHGLYMGNLNLFHRPLRVGKWAVTYEEWPEEEYPTYANGPGYII 827
Query: 405 SGDIAKFIV 431
S D+A F+V
Sbjct: 828 SNDVANFVV 854
Score = 141 bits (303), Expect = 3e-033
Identities = 73/142 (51%), Positives = 85/142 (59%)
Frame = -1 / -1
Query: 433 DTMNFAMSPEIT*PGPFAYXXXXXXXXXXSVTAHFPVLNGLCRRLRFPIYNGLSPLFNLM 254
DT FA S E+ PGPFA VTAH P +GL +RLR PIY + L+
Sbjct: 856 DTTKFATSFEMIYPGPFA*VGYSSSGHSSYVTAHLPTRSGLWKRLRLPIYKPCTWGVLLI 677
Query: 253 RLRTTSTLTNVSSSHFMMFAAVKFWTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFFN 74
T STLT VSSSHF+ + A F TPYSQIA V TTSS*RSING + +SP SASFF+
Sbjct: 676 SFNTVSTLTKVSSSHFITYFAEIFRTPYSQIATVFNTTSS*RSINGTIIISP*KSASFFS 497
Query: 73 ITLTSFLEFSATKNRAPAYLPG 8
TLTSFL F + R A + G
Sbjct: 496 CTLTSFLAFISATAR*KADIRG 431
Score = 118 bits (253), Expect = 3e-026
Identities = 49/128 (38%), Positives = 78/128 (60%)
Frame = -3 / -3
Query: 431 HDEFCYVTRNHIAWSVCIWRVDILRPLLVCNCPFSSPQWSVQEVKVPHI*WFVTIVQLDA 252
+++ Y+ N I+WS+CI V P L+C PF++ +W V+++KV HI D
Sbjct: 854 YNKIRYII*NDISWSICISGVLFFWPFLICYSPFANTEWPVEKIKVTHIQAMYMGSSFDF 675
Query: 251 SQNYIYSHKCIIVTFHDVCSSQVLDPILTDSNCLKNNQLIAVYKWQNDNVSKVFCFFLQH 72
Q I+SH+ II+TFH++ + +PI T+ N L++ + I V+KW ++N+S CF L
Sbjct: 674 LQYSIHSHEGIIITFHNIFCRNISNPIFTNCNSLQHYKFITVHKWNDNNISIKICFIL*L 495
Query: 71 YIDLLPRI 48
+IDLLP I
Sbjct: 494 HIDLLPCI 471
>gb|DT638404.1|DT638404 EST1153335 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMHE72 3' end, mRNA sequence
Length = 824
Score = 200 bits (432), Expect = 5e-051
Identities = 78/117 (66%), Positives = 89/117 (76%)
Frame = +3 / +2
Query: 207 KCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDEEWPEDIYPPYAN 386
+CDDDTFVRVD VL+ IK LYMGNLNL HRPLR GKWAVT EEWPE+ YP YAN
Sbjct: 8 ECDDDTFVRVDTVLKEIKRTPHVHGLYMGNLNLFHRPLRVGKWAVTYEEWPEEEYPTYAN 187
Query: 387 GPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVEKFNSTXXVXYSHSWKXXXY 557
GPGY+IS D+A F+VSQ AN LF MEDVSMG+WVE+FN++ V YSH+WK Y
Sbjct: 188 GPGYIISNDVANFVVSQHANHTLRLFKMEDVSMGMWVEQFNASSRVQYSHNWKFCQY 358
Score = 107 bits (229), Expect = 5e-023
Identities = 58/116 (50%), Positives = 64/116 (55%)
Frame = -1 / -3
Query: 556 YXXXFQLXEYXTXXVELNFSTHRPMLTSSIXNSXXL*LARCDTMNFAMSPEIT*PGPFAY 377
Y FQL EY T LN STH PMLTSSI S +*LA DT FA S E+ PGPFA
Sbjct: 357 YWQNFQLCEY*TREEALNCSTHIPMLTSSILKSLKV*LAC*DTTKFATSFEMIYPGPFA* 178
Query: 376 XXXXXXXXXXSVTAHFPVLNGLCRRLRFPIYNGLSPLFNLMRLRTTSTLTNVSSSH 209
VTAH P +GL +RLR PIY + L+ T STLT VSSSH
Sbjct: 177 VGYSSSGHSSYVTAHLPTRSGLWKRLRLPIYKPCTWGVLLISFNTVSTLTKVSSSH 10
>gb|DR685171.1|DR685171 EST1075248 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAB082 3' end, mRNA sequence
Length = 625
Score = 128 bits (274), Expect(3) = 3e-048
Identities = 48/65 (73%), Positives = 61/65 (93%)
Frame = +3 / +2
Query: 45 LNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQNLTAANIMKCDDDT 224
+N+RKEVNV LK EA+++GDI+I+PF+DRYELVVLKT+AICEYGV+N++A +MKCDDDT
Sbjct: 254 MNARKEVNVQLKNEADFYGDIIIVPFMDRYELVVLKTVAICEYGVRNISAKYVMKCDDDT 433
Query: 225 FVRVD 239
FVRVD
Sbjct: 434 FVRVD 448
Score = 94.1 bits (199), Expect(3) = 6e-026
Identities = 47/77 (61%), Positives = 54/77 (70%)
Frame = -1 / -2
Query: 238 STLTNVSSSHFMMFAAVKFWTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFFNITLTS 59
STLT VSSSHF+ + A F TPYSQIA V TTSS*RSING + +SP SASFF+ TLTS
Sbjct: 447 STLTKVSSSHFITYFAEIFRTPYSQIATVFNTTSS*RSINGTIIISP*KSASFFSCTLTS 268
Query: 58 FLEFSATKNRAPAYLPG 8
FL F + R A + G
Sbjct: 267 FLAFISATAR*KADIRG 217
Score = 54.2 bits (112), Expect(3) = 3e-048
Identities = 23/38 (60%), Positives = 26/38 (68%)
Frame = +3 / +3
Query: 240 VVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDEE 353
+VL+ IK LYMGNLNL H PLR GKWAVT E+
Sbjct: 450 LVLKEIKRTPHVHGLYMGNLNLFHTPLRVGKWAVTYED 563
Score = 47.8 bits (98), Expect(3) = 3e-048
Identities = 15/21 (71%), Positives = 18/21 (85%)
Frame = +3 / +2
Query: 354 WPEDIYPPYANGPGYVISGDI 416
WPE+ YP YANGPGY+IS D+
Sbjct: 563 WPEEEYPTYANGPGYIISNDV 625
Score = 36.8 bits (74), Expect(3) = 6e-026
Identities = 11/27 (40%), Positives = 20/27 (74%)
Frame = -3 / -3
Query: 365 ILRPLLVCNCPFSSPQWSVQEVKVPHI 285
+L +L+C PF++ +W V+++KV HI
Sbjct: 575 LLLAILICYSPFANTEWRVEKIKVTHI 495
Score = 24.0 bits (46), Expect(3) = 6e-026
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -3 / -1
Query: 416 YVTRNHIAWSVCI 378
Y+ N I+WS+CI
Sbjct: 625 YII*NDISWSICI 587
>gb|DR116979.1|DR116979 RTMG1_4_B01.b1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_4_B01_A029 3', mRNA sequence
Length = 725
Score = 175 bits (377), Expect = 2e-043
Identities = 67/101 (66%), Positives = 78/101 (77%)
Frame = +3 / +3
Query: 243 VLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDEEWPEDIYPPYANGPGYVISGDIAK 422
VL+ + + KPLY+GNLN H+PLR GKWAVT EEWPED YPPYANGPGY+I+ DIA
Sbjct: 3 VLKEVNRLSHGKPLYIGNLNYFHKPLRVGKWAVTYEEWPEDDYPPYANGPGYIITRDIAN 182
Query: 423 FIVSQRANQXXXLFXMEDVSMGLWVEKFNSTXXVXYSHSWK 545
FIVSQ NQ LF MEDVSMG+WVE+FN++ V YSH WK
Sbjct: 183 FIVSQHKNQTLRLFKMEDVSMGMWVEQFNASTPVQYSHDWK 305
>gb|DR694121.1|DR694121 EST1084211 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE837 3' end, mRNA sequence
Length = 746
Score = 171 bits (367), Expect = 5e-042
Identities = 64/92 (69%), Positives = 73/92 (79%)
Frame = +3 / +3
Query: 282 LYMGNLNLLHRPLRTGKWAVTDEEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXXXL 461
LYMGNLNL HRPLR GKWAVT EEWPE+ YP YANGPGY+IS D+A F+VSQ AN L
Sbjct: 6 LYMGNLNLFHRPLRVGKWAVTYEEWPEEEYPTYANGPGYIISNDVANFVVSQHANHTLRL 185
Query: 462 FXMEDVSMGLWVEKFNSTXXVXYSHSWKXXXY 557
F MEDVSMG+WVE+FN++ V YSH+WK Y
Sbjct: 186 FKMEDVSMGMWVEQFNASSRVQYSHNWKFCQY 281
>gb|DR111377.1|DR111377 RTS1_17_F12.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_17_F12_A029 3', mRNA sequence
Length = 674
Score = 140 bits (300), Expect = 8e-033
Identities = 53/73 (72%), Positives = 59/73 (80%)
Frame = +3 / +2
Query: 327 GKWAVTDEEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVEKF 506
GKWAVT EEWPED YPPYANGPGY+I+ DIA FIVSQ NQ LF MEDVSMG+WVE+F
Sbjct: 5 GKWAVTYEEWPEDDYPPYANGPGYIITRDIANFIVSQHKNQTLRLFKMEDVSMGMWVEQF 184
Query: 507 NSTXXVXYSHSWK 545
N++ V YSH WK
Sbjct: 185 NASTPVQYSHDWK 223
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 305,764,507
Number of Sequences: 355925
Number of extensions: 4466326
Number of successful extensions: 45263
Number of sequences better than 1.0e-020: 8
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 7544026230
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)