TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM12g02.yg.2.1
(480 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ696956.1|BQ696956 NXPV_047_C11_F NXPV (Nsf Xylem Plani... 214 3e-055
gb|CO159016.1|CO159016 FLD1_10_F07.g1_A029 Root flooded Pin... 201 2e-051
gb|BG317521.1|BG317521 NXPV_002_G06_F NXPV (Nsf Xylem Plani... 181 3e-045
gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus adde... 165 2e-040
>gb|BQ696956.1|BQ696956 NXPV_047_C11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_047_C11 5' similar to Arabidopsis
thaliana sequence At5g13000 callose synthase catalytic
subunit -like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 601
Score = 214 bits (462), Expect = 3e-055
Identities = 83/147 (56%), Positives = 110/147 (74%)
Frame = -3 / +2
Query: 460 VXNQQFIIQLGFFTALPMIIENSLEQGFLPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGR 281
V Q ++Q+G ALPM++E LE+GF A+ DF MQ+ +SVF+TFS+GTK+HY+GR
Sbjct: 137 VLASQSLVQIGLLMALPMVMEIGLERGFRSALSDFVIMQLQLASVFFTFSLGTKTHYFGR 316
Query: 280 TILHGGAKYRATGRGFVVQHKSFAENYRLYARGHFIKAIELGIILTVYAAHSVIAKNTLV 101
TILHGGAKYRATGRGFVV+H+ +AENYRLY+R HF K +EL ++L VY + AK+ +
Sbjct: 317 TILHGGAKYRATGRGFVVRHEKYAENYRLYSRSHFTKGLELMMLLFVYNIYCSAAKDFVS 496
Query: 100 YIXXXISSWFLVVSWIMAPFAFNPSGF 20
Y+ +S WFLVV+W+ APF FNP F
Sbjct: 497 YLLITLSMWFLVVTWLFAPFLFNPXXF 577
Score = 106 bits (226), Expect(2) = 1e-025
Identities = 54/103 (52%), Positives = 63/103 (61%)
Frame = +3 / -2
Query: 141 TVNIIPSSIAFMKWPLAYSL*FSAKLLCCTTKPRPVARYFAPP*RIVRP**WLLVPMENV 320
T I SS F+KW L SL*FSA C TTKPRPVA YFAPP +IV P * + VP ENV
Sbjct: 456 TKRSIISSSPFVKWLLE*SL*FSAYFSCRTTKPRPVALYFAPPCKIVLPK**VFVPRENV 277
Query: 321 *NTDEKFICIVKKSQIAGRKPCSREFSIIIGNAVKKPSCMMNC 449
T+ CI+ KS A RKP S+ SI IG A+ +P +C
Sbjct: 276 KKTEASCSCIITKSLNADRKPRSKPISITIGKAISRPI*TRDC 148
Score = 29.0 bits (57), Expect(2) = 1e-025
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2 / -1
Query: 20 KTRRIKSKWSHNP*YHQKP 76
KT I+ +WS P +HQKP
Sbjct: 577 KTXGIEQEWSKQPCHHQKP 521
>gb|CO159016.1|CO159016 FLD1_10_F07.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_10_F07_A029 5', mRNA sequence
Length = 796
Score = 201 bits (434), Expect = 2e-051
Identities = 76/130 (58%), Positives = 97/130 (74%)
Frame = -3 / +3
Query: 391 LEQGFLPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSF 212
LE+GF A+ DF MQ+ ++VF+TFS+GTK+HYYGRT+LHGGAKYR TGRGFVV H F
Sbjct: 3 LERGFRKALSDFVIMQLQMAAVFFTFSLGTKTHYYGRTLLHGGAKYRGTGRGFVVFHAKF 182
Query: 211 AENYRLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYIXXXISSWFLVVSWIMAPFAFN 32
AENYR Y+R HF+K +EL ++L VY +S + T+ YI S WF+V +W+ APF FN
Sbjct: 183 AENYRFYSRSHFVKGLELMLLLIVYTVYSQAHRKTVPYILITYSMWFMVGAWLFAPFLFN 362
Query: 31 PSGFDWLKTV 2
PSGF+W K V
Sbjct: 363 PSGFEWQKIV 392
>gb|BG317521.1|BG317521 NXPV_002_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_002_G06 5' similar to Arabidopsis
thaliana sequence At5g13000 callose synthase catalytic
subunit -like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 421
Score = 181 bits (389), Expect = 3e-045
Identities = 66/109 (60%), Positives = 84/109 (77%)
Frame = -3 / +1
Query: 328 VFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARGHFIKAIELGII 149
VF+TFS+GTK+HYYGRT+LHGGAKYRATGRGFVV H FA+NYR Y+R HF+K +EL ++
Sbjct: 1 VFFTFSLGTKTHYYGRTLLHGGAKYRATGRGFVVFHAKFADNYRFYSRSHFVKGLELMLL 180
Query: 148 LTVYAAHSVIAKNTLVYIXXXISSWFLVVSWIMAPFAFNPSGFDWLKTV 2
L VY + +NT+ Y+ S WF+V +W+ APF FNPSGF+W K V
Sbjct: 181 LIVYNVYGQPYRNTIAYLLITFSMWFMVGTWLFAPFLFNPSGFEWQKIV 327
>gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_25_F10_A029 5', mRNA sequence
Length = 753
Score = 165 bits (354), Expect = 2e-040
Identities = 63/94 (67%), Positives = 78/94 (82%)
Frame = -3 / +1
Query: 454 NQQFIIQLGFFTALPMIIENSLEQGFLPAIWDFFTMQMNFSSVFYTFSMGTKSHYYGRTI 275
N QF+ Q+G FTA+PMI+ LEQGFL AI FFTMQ+ S+F+TFS+GT++HY+GRTI
Sbjct: 472 NTQFLFQIGIFTAVPMIMGFILEQGFLKAIVSFFTMQLQLCSIFFTFSLGTRTHYFGRTI 651
Query: 274 LHGGAKYRATGRGFVVQHKSFAENYRLYARGHFI 173
LHGGA Y+ATGRGFVVQH FA+NYRLY+R HF+
Sbjct: 652 LHGGAXYKATGRGFVVQHIKFADNYRLYSRSHFV 753
Score = 105 bits (223), Expect = 3e-022
Identities = 52/91 (57%), Positives = 58/91 (63%)
Frame = +3 / -2
Query: 177 KWPLAYSL*FSAKLLCCTTKPRPVARYFAPP*RIVRP**WLLVPMENV*NTDEKFICIVK 356
KW SL* SA L+C TTKP PVA Y APP*RIVRP * +LVP ENV N + CIVK
Sbjct: 749 KWLRE*SL*LSANLICWTTKPLPVALYXAPP*RIVRPK**VLVPRENVKNIEHSCSCIVK 570
Query: 357 KSQIAGRKPCSREFSIIIGNAVKKPSCMMNC 449
K +A + PCS+ IIIG AV P NC
Sbjct: 569 KLTMAFKNPCSKMKPIIIGTAVNIPIWNKNC 477
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 265,317,129
Number of Sequences: 355925
Number of extensions: 3874851
Number of successful extensions: 62167
Number of sequences better than 1.0e-020: 7
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 5861545560
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)