TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG16f05.yg.2.1
(585 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciat... 82 3e-031
gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo li... 80 1e-028
gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo li... 80 1e-028
>gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP032F08, mRNA sequence
Length = 674
Score = 81.7 bits (172), Expect(2) = 3e-031
Identities = 37/100 (37%), Positives = 53/100 (53%)
Frame = -1 / +1
Query: 552 VLXXXGDXGXXXLGXXXAIQXKNXXLFEDFNSDSTKRHSLTAVADSLKNRSVFHISYNPR 373
VL + G LG A Q ++ +S L AVA ++ +S+FHI YNPR
Sbjct: 235 VLFLRDENGELRLGIRRASQQQSSVPSSVLSSHGIHSGVLAAVAHAVATKSMFHIYYNPR 414
Query: 372 ATASEYIIPYAKFLKSLNHPVCIGARINFQCHNEDVSERR 253
+ +E++IPY K++KS NH IG R + ED +ERR
Sbjct: 415 TSPTEFVIPYHKYVKSFNHSFSIGMRFKMRFETEDATERR 534
Score = 73.5 bits (154), Expect(2) = 3e-031
Identities = 26/47 (55%), Positives = 34/47 (72%)
Frame = -1 / +1
Query: 258 RRSGVVVRISEIDPMKWPGSKWRSLLVRWEDGAECNGQDRVSPWEIE 118
R +G +V I ++DPM+WP S+WRS V W++ A QDRVSPWEIE
Sbjct: 532 RYTGTIVGIGDVDPMRWPNSRWRSFKVGWDEHAAQXRQDRVSPWEIE 672
>gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAB186 3' end, mRNA sequence
Length = 841
Score = 80.3 bits (169), Expect(2) = 1e-028
Identities = 33/94 (35%), Positives = 52/94 (55%)
Frame = -1 / +2
Query: 537 GDXGXXXLGXXXAIQXKNXXLFEDFNSDSTKRHSLTAVADSLKNRSVFHISYNPRATASE 358
G+ G LG A + K+ L ++ + L A A ++ +S+FH+ YNPR + +E
Sbjct: 5 GENGELRLGIRRATRQKSGILSSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTSPAE 184
Query: 357 YIIPYAKFLKSLNHPVCIGARINFQCHNEDVSER 256
+IIPY K++KS P+ IG R + ED +ER
Sbjct: 185 FIIPYQKYVKSCKQPLSIGMRFKMRFETEDTAER 286
Score = 65.7 bits (137), Expect(2) = 1e-028
Identities = 23/45 (51%), Positives = 31/45 (68%)
Frame = -1 / +2
Query: 252 SGVVVRISEIDPMKWPGSKWRSLLVRWEDGAECNGQDRVSPWEIE 118
+G++ I + DP +WPGSKWR L V W++ A Q+RVS WEIE
Sbjct: 293 TGMITAIGDADPARWPGSKWRLLKVEWDEHAANEQQERVSAWEIE 427
>gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFN79 3' end, mRNA sequence
Length = 783
Score = 80.3 bits (169), Expect(2) = 1e-028
Identities = 33/94 (35%), Positives = 52/94 (55%)
Frame = -1 / +2
Query: 537 GDXGXXXLGXXXAIQXKNXXLFEDFNSDSTKRHSLTAVADSLKNRSVFHISYNPRATASE 358
G+ G LG A + K+ L ++ + L A A ++ +S+FH+ YNPR + +E
Sbjct: 5 GENGELRLGIRRATRQKSGILSSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTSPAE 184
Query: 357 YIIPYAKFLKSLNHPVCIGARINFQCHNEDVSER 256
+IIPY K++KS P+ IG R + ED +ER
Sbjct: 185 FIIPYQKYVKSCKQPLSIGMRFKMRFETEDTAER 286
Score = 65.7 bits (137), Expect(2) = 1e-028
Identities = 23/45 (51%), Positives = 31/45 (68%)
Frame = -1 / +2
Query: 252 SGVVVRISEIDPMKWPGSKWRSLLVRWEDGAECNGQDRVSPWEIE 118
+G++ I + DP +WPGSKWR L V W++ A Q+RVS WEIE
Sbjct: 293 TGMITAIGDADPARWPGSKWRLLKVEWDEHAANEQQERVSAWEIE 427
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 277,275,719
Number of Sequences: 355925
Number of extensions: 3723648
Number of successful extensions: 39048
Number of sequences better than 1.0e-020: 4
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 7761120510
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)