TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG11e12.yg.2.1
(636 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT638905.1|DT638905 EST1153836 Normalized pine embryo li... 145 1e-037
gb|DR694955.1|DR694955 EST1085048 Normalized pine embryo li... 145 1e-037
gb|BQ695602.1|BQ695602 NXPV_030_E03_F NXPV (Nsf Xylem Plani... 119 4e-030
gb|BQ696899.1|BQ696899 NXPV_046_F07_F NXPV (Nsf Xylem Plani... 101 7e-025
gb|BI202722.1|BI202722 NXPV_097_D09_F NXPV (Nsf Xylem Plani... 113 3e-024
>gb|DT638905.1|DT638905 EST1153836 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMHK79 3' end, mRNA sequence
Length = 821
Score = 145 bits (311), Expect(2) = 1e-037
Identities = 63/127 (49%), Positives = 84/127 (66%)
Frame = +2 / +1
Query: 200 SQWSGFLDGIRGNSGENYAVVSKRTEHWQGLEQDITNQVSTGTVYVVSAFVRVDGIVQGQ 379
S S + D I+ SG +AV S RT+ WQGLEQDIT ++ Y V AFVR+ G Q
Sbjct: 166 SDGSDYADEIKAESGSQFAVASNRTQCWQGLEQDITPRLLPQVTYKVLAFVRIWGSTQQT 345
Query: 380 VEVKATLRLQNADGSTHYNPVGSVVASKEKWNKLEGSFSLTNMPKNVVFYLEGPPAGVDL 559
++ ATLRL+N + + + VG V ASKE+W KLEG+FSL ++P V+FYLEGP G+DL
Sbjct: 346 ADLLATLRLENPNSAPTFLMVGRVTASKERWEKLEGTFSLKSLPYRVIFYLEGPLPGLDL 525
Query: 560 VIDSVTV 580
+ID V +
Sbjct: 526 LIDRVHI 546
Score = 33.1 bits (66), Expect(2) = 1e-037
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 125 NIILNYDFSKGLHPWNPIRCHAY 193
N I+N+DFSKGL W+ C+ +
Sbjct: 94 NAIINHDFSKGLLSWHANSCNGF 162
>gb|DR694955.1|DR694955 EST1085048 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAEI80 3' end, mRNA sequence
Length = 811
Score = 145 bits (311), Expect(2) = 1e-037
Identities = 63/127 (49%), Positives = 84/127 (66%)
Frame = +2 / +1
Query: 200 SQWSGFLDGIRGNSGENYAVVSKRTEHWQGLEQDITNQVSTGTVYVVSAFVRVDGIVQGQ 379
S S + D I+ SG +AV S RT+ WQGLEQDIT ++ Y V AFVR+ G Q
Sbjct: 166 SDGSDYADEIKAESGSQFAVASNRTQCWQGLEQDITPRLLPQVTYKVLAFVRIWGSTQQT 345
Query: 380 VEVKATLRLQNADGSTHYNPVGSVVASKEKWNKLEGSFSLTNMPKNVVFYLEGPPAGVDL 559
++ ATLRL+N + + + VG V ASKE+W KLEG+FSL ++P V+FYLEGP G+DL
Sbjct: 346 ADLLATLRLENPNSAPTFLMVGRVTASKERWEKLEGTFSLKSLPYRVIFYLEGPLPGLDL 525
Query: 560 VIDSVTV 580
+ID V +
Sbjct: 526 LIDRVHI 546
Score = 33.1 bits (66), Expect(2) = 1e-037
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 125 NIILNYDFSKGLHPWNPIRCHAY 193
N I+N+DFSKGL W+ C+ +
Sbjct: 94 NAIINHDFSKGLLSWHANSCNGF 162
>gb|BQ695602.1|BQ695602 NXPV_030_E03_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_030_E03 5' similar to Arabidopsis
thaliana sequence At1g10050 putative xylan endohydrolase
see http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 530
Score = 119 bits (255), Expect(2) = 4e-030
Identities = 52/110 (47%), Positives = 70/110 (63%)
Frame = +2 / +1
Query: 200 SQWSGFLDGIRGNSGENYAVVSKRTEHWQGLEQDITNQVSTGTVYVVSAFVRVDGIVQGQ 379
S S + D I+ SG +AV S RT+ WQGLEQDIT ++ Y V AFVR+ G Q
Sbjct: 163 SDGSDYADEIKAESGSQFAVASNRTQCWQGLEQDITPRLLPQVTYKVLAFVRIWGSTQQT 342
Query: 380 VEVKATLRLQNADGSTHYNPVGSVVASKEKWNKLEGSFSLTNMPKNVVFY 529
++ ATLRL+N + + + V V ASKE+W KLEG+FSL ++P V+FY
Sbjct: 343 ADLLATLRLENPNSAPTFLMVXXVTASKERWEKLEGTFSLKSLPYRVIFY 492
Score = 33.1 bits (66), Expect(2) = 4e-030
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 125 NIILNYDFSKGLHPWNPIRCHAY 193
N I+N+DFSKGL W+ C+ +
Sbjct: 91 NAIINHDFSKGLLSWHANSCNGF 159
>gb|BQ696899.1|BQ696899 NXPV_046_F07_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_046_F07 5' similar to Arabidopsis
thaliana sequence At1g10050 putative xylan endohydrolase
see http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 496
Score = 101 bits (216), Expect(2) = 7e-025
Identities = 46/100 (46%), Positives = 60/100 (60%)
Frame = +2 / +1
Query: 200 SQWSGFLDGIRGNSGENYAVVSKRTEHWQGLEQDITNQVSTGTVYVVSAFVRVDGIVQGQ 379
S S + D I+ SG +AV S RT+ WQGLEQDIT ++ Y V AFVR+ G Q
Sbjct: 163 SDGSDYADEIKAESGSQFAVASNRTQCWQGLEQDITPRLLPQVTYKVLAFVRIWGSTQQT 342
Query: 380 VEVKATLRLQNADGSTHYNPVGSVVASKEKWNKLEGSFSL 499
++ ATLRL+N + + + V ASKE+W KL G FSL
Sbjct: 343 ADLLATLRLENPNSAPTFLXVXXXTASKERWEKLXGXFSL 462
Score = 33.1 bits (66), Expect(2) = 7e-025
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 125 NIILNYDFSKGLHPWNPIRCHAY 193
N I+N+DFSKGL W+ C+ +
Sbjct: 91 NAIINHDFSKGLLSWHANSCNGF 159
>gb|BI202722.1|BI202722 NXPV_097_D09_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_097_D09 5' similar to Arabidopsis
thaliana sequence At1g58370 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 512
Score = 113 bits (241), Expect = 3e-024
Identities = 50/110 (45%), Positives = 71/110 (64%)
Frame = +2 / +2
Query: 251 YAVVSKRTEHWQGLEQDITNQVSTGTVYVVSAFVRVDGIVQGQVEVKATLRLQNADGSTH 430
+A++S RT++WQGLEQDIT ++ Y + A VRV+G Q V ATLRL+N + S +
Sbjct: 5 HALISNRTDYWQGLEQDITTRLIPKMQYKILASVRVEGSPQSIHSVMATLRLENNNASPN 184
Query: 431 YNPVGSVVASKEKWNKLEGSFSLTNMPKNVVFYLEGPPAGVDLVIDSVTV 580
+ + V AS KW +L G+FSL +P+ VFYL GPPAG + + SV +
Sbjct: 185 FLSLARVKASVGKWEQLMGAFSLEVIPEXAVFYLXGPPAGFNFSLRSVVI 334
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 350,173,130
Number of Sequences: 355925
Number of extensions: 5289013
Number of successful extensions: 48761
Number of sequences better than 1.0e-020: 8
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 8683771200
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)