TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN10e11.xg.2.2
(581 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT636879.1|DT636879 EST1151810 Normalized pine embryo li... 122 2e-035
gb|DR691945.1|DR691945 EST1082032 Normalized pine embryo li... 122 2e-035
gb|DR686435.1|DR686435 EST1076513 Normalized pine embryo li... 121 4e-035
gb|DR693591.1|DR693591 EST1083680 Normalized pine embryo li... 118 2e-034
dbj|BD273093.1| Materials and methods for the modification ... 117 3e-034
gb|DT633502.1|DT633502 EST1148433 Normalized pine embryo li... 68 3e-031
gb|CX713935.1|CX713935 RTPQ1_15_D05.b1_A032 Roots treated w... 100 7e-029
gb|CF672887.1|CF672887 RTCNT1_74_D06.g1_A029 Root control P... 79 9e-023
gb|CX645971.1|CX645971 COLD1_6_A08.g1_A029 Root cold Pinus ... 106 1e-022
gb|CO198411.1|CO198411 GEO1_13_B02.g1_A029 Root gravitropis... 90 2e-022
gb|DR168008.1|DR168008 RTPHOS1_22_C11.g1_A029 Roots minus p... 83 7e-022
gb|DR088681.1|DR088681 RTAL1_3_F02.g1_A029 Roots plus added... 90 7e-021
>gb|DT636879.1|DT636879 EST1151810 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGX11 3' end, mRNA sequence
Length = 829
Score = 122 bits (261), Expect(2) = 2e-035
Identities = 51/66 (77%), Positives = 56/66 (84%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GET RE+IKRH+HF+ALQL LK MKVLDVGCGIGGPL EIARFS TSITGL NN Y
Sbjct: 218 RWKGETLRESIKRHEHFLALQLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAY 397
Query: 396 HVSRGK 413
+SRGK
Sbjct: 398 QISRGK 415
Score = 48.7 bits (100), Expect(2) = 2e-035
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 158 YDLATSFYEYGWGESFHFA 214
>gb|DR691945.1|DR691945 EST1082032 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWADG12 3' end, mRNA sequence
Length = 832
Score = 122 bits (261), Expect(2) = 2e-035
Identities = 51/66 (77%), Positives = 56/66 (84%)
Frame = +3 / +3
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GET RE+IKRH+HF+ALQL LK MKVLDVGCGIGGPL EIARFS TSITGL NN Y
Sbjct: 210 RWKGETLRESIKRHEHFLALQLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAY 389
Query: 396 HVSRGK 413
+SRGK
Sbjct: 390 QISRGK 407
Score = 48.7 bits (100), Expect(2) = 2e-035
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +3
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 150 YDLATSFYEYGWGESFHFA 206
>gb|DR686435.1|DR686435 EST1076513 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABG04 3' end, mRNA sequence
Length = 808
Score = 121 bits (258), Expect(2) = 4e-035
Identities = 51/66 (77%), Positives = 56/66 (84%)
Frame = +3 / +3
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GET RE+IKRH+HF+ALQL LK MKVLDVGCGIGGPL EIARFS TSITGL NN Y
Sbjct: 456 RWRGETLRESIKRHEHFLALQLCLKPAMKVLDVGCGIGGPLREIARFSWTSITGLNNNAY 635
Query: 396 HVSRGK 413
+SRGK
Sbjct: 636 QISRGK 653
Score = 48.7 bits (100), Expect(2) = 4e-035
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +3
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 396 YDLATSFYEYGWGESFHFA 452
>gb|DR693591.1|DR693591 EST1083680 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE147 3' end, mRNA sequence
Length = 733
Score = 118 bits (253), Expect(2) = 2e-034
Identities = 49/66 (74%), Positives = 55/66 (83%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GET RE+IKRH+HF+AL L LK MKVLDVGCGIGGPL EIARFS TSITGL NN Y
Sbjct: 151 RWKGETLRESIKRHEHFLALHLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAY 330
Query: 396 HVSRGK 413
+SRG+
Sbjct: 331 QISRGR 348
Score = 48.7 bits (100), Expect(2) = 2e-034
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 91 YDLATSFYEYGWGESFHFA 147
>dbj|BD273093.1| Materials and methods for the modification of isoprenoid content,
compostition and metabolism
Length = 582
Score = 117 bits (251), Expect(2) = 3e-034
Identities = 49/65 (75%), Positives = 54/65 (83%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GET RE+IKRH+HF+AL L LK MKVLDVGCGIGGPL EIARFS TSITGL NN Y
Sbjct: 388 RWKGETLRESIKRHEHFLALHLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAY 567
Query: 396 HVSRG 410
+SRG
Sbjct: 568 QISRG 582
Score = 48.7 bits (100), Expect(2) = 3e-034
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 328 YDLATSFYEYGWGESFHFA 384
>gb|DT633502.1|DT633502 EST1148433 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFV57 3' end, mRNA sequence
Length = 857
Score = 67.5 bits (141), Expect(3) = 3e-031
Identities = 27/36 (75%), Positives = 31/36 (86%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCG 323
R+ GET RE+IKRH+HF+ALQL LK MKVLDVGCG
Sbjct: 464 RWRGETLRESIKRHEHFLALQLCLKPAMKVLDVGCG 571
Score = 59.3 bits (123), Expect(3) = 3e-031
Identities = 25/31 (80%), Positives = 26/31 (83%)
Frame = +3 / +3
Query: 321 GIGGPLIEIARFSSTSITGLTNNDYHVSRGK 413
GIGGPL EIARFS TSITGL NN Y +SRGK
Sbjct: 570 GIGGPLREIARFSWTSITGLNNNAYQISRGK 662
Score = 48.7 bits (100), Expect(3) = 3e-031
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 404 YDLATSFYEYGWGESFHFA 460
>gb|CX713935.1|CX713935 RTPQ1_15_D05.b1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_15_D05_A032 3', mRNA sequence
Length = 800
Score = 100 bits (212), Expect(2) = 7e-029
Identities = 42/54 (77%), Positives = 46/54 (85%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITG 377
R+ GET RE+IKRH+HF+AL L LK MKVLDVGCGIGGPL EIARFS TSITG
Sbjct: 638 RWKGETLRESIKRHEHFLALHLCLKPAMKVLDVGCGIGGPLREIARFSRTSITG 799
Score = 48.7 bits (100), Expect(2) = 7e-029
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 578 YDLATSFYEYGWGESFHFA 634
>gb|CF672887.1|CF672887 RTCNT1_74_D06.g1_A029 Root control Pinus taeda cDNA clone
RTCNT1_74_D06_A029 5', mRNA sequence
Length = 740
Score = 79.4 bits (167), Expect(2) = 9e-023
Identities = 33/64 (51%), Positives = 44/64 (68%)
Frame = +3 / +3
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ E+ ++ R + ++AL+L L KGM+VLDVGCGIGGP IARFS +I GL NN Y
Sbjct: 246 RHRFESFEASVSRLEFYVALKLKLDKGMRVLDVGCGIGGPARAIARFSQATIIGLNNNSY 425
Query: 396 HVSR 407
+ R
Sbjct: 426 QLQR 437
Score = 48.7 bits (100), Expect(2) = 9e-023
Identities = 17/19 (89%), Positives = 17/19 (89%)
Frame = +3 / +3
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYEYGWG SFHFA
Sbjct: 186 YDLVTHFYEYGWGQSFHFA 242
>gb|CX645971.1|CX645971 COLD1_6_A08.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_6_A08_A029 5', mRNA sequence
Length = 691
Score = 106 bits (226), Expect = 1e-022
Identities = 44/55 (80%), Positives = 47/55 (85%)
Frame = +3 / +2
Query: 249 KRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDYHVSRGK 413
KRH+HF+ALQL LK MKVLDVGCGIGGPL EIARFS TSITGL NN Y +SRGK
Sbjct: 2 KRHEHFLALQLCLKPAMKVLDVGCGIGGPLREIARFSRTSITGLNNNAYQISRGK 166
>gb|CO198411.1|CO198411 GEO1_13_B02.g1_A029 Root gravitropism April 2003 test Pinus taeda
cDNA clone GEO1_13_B02_A029 5', mRNA sequence
Length = 670
Score = 89.5 bits (189), Expect(2) = 2e-022
Identities = 33/64 (51%), Positives = 44/64 (68%)
Frame = +3 / +2
Query: 225 GETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDYHVS 404
GE AI RH+H++A +G+K GMKVLDVGCG+GGP EI +F+ +TGL N+Y V
Sbjct: 368 GEDFNRAIARHEHYLAHNIGIKPGMKVLDVGCGVGGPAREIVKFTGAHVTGLNLNEYQVQ 547
Query: 405 RGKV 416
R +
Sbjct: 548 RATI 559
Score = 37.7 bits (76), Expect(2) = 2e-022
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHF 56
Y+L T YEYGW SFHF
Sbjct: 299 YNLATDLYEYGWSQSFHF 352
>gb|DR168008.1|DR168008 RTPHOS1_22_C11.g1_A029 Roots minus phosphorous Pinus taeda cDNA
clone RTPHOS1_22_C11_A029 5', mRNA sequence
Length = 600
Score = 83.1 bits (175), Expect(2) = 7e-022
Identities = 35/83 (42%), Positives = 49/83 (59%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
++ GE EA+KR + ++AL+ +K+G KVLD+GCGIGGP+ IARF+ +ITG N Y
Sbjct: 233 QFKGEPYTEALKRQEFYLALRAHMKEGSKVLDIGCGIGGPMRNIARFTGANITGFNLNAY 412
Query: 396 HVSRGKVCILAFFLEHXNCIAYG 464
V R L H + G
Sbjct: 413 QVKRATELNAKAGLSHLCTVVQG 481
Score = 41.8 bits (85), Expect(2) = 7e-022
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE GWG+ FHFA
Sbjct: 173 YDLATDFYEKGWGEHFHFA 229
>gb|DR088681.1|DR088681 RTAL1_3_F02.g1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_3_F02_A029 5', mRNA sequence
Length = 661
Score = 90.0 bits (190), Expect(2) = 7e-021
Identities = 32/61 (52%), Positives = 45/61 (73%)
Frame = +3 / +2
Query: 225 GETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDYHVS 404
GE ++ RH+H++AL++GLK GM+VLDVGCG+GGP EI+RF+ + GL NN + V
Sbjct: 245 GEAFNASLARHEHYLALRMGLKPGMRVLDVGCGVGGPAREISRFADVDVVGLNNNAFQVG 424
Query: 405 R 407
R
Sbjct: 425 R 427
Score = 31.8 bits (63), Expect(2) = 7e-021
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YD T YE+ W SFHF+
Sbjct: 176 YDGATELYEFAWAQSFHFS 232
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 292,295,513
Number of Sequences: 355925
Number of extensions: 4141103
Number of successful extensions: 45214
Number of sequences better than 1.0e-020: 13
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 7706846940
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)