TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBD3a09.xg.3.1
(1105 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO161136.1|CO161136 FLD1_26_H05.g1_A029 Root flooded Pin... 222 2e-066
gb|DR070788.1|DR070788 RTDK1_15_C12.g1_A029 Roots, dark Pin... 221 3e-066
gb|DR070708.1|DR070708 RTDK1_15_C12.b1_A029 Roots, dark Pin... 221 1e-062
gb|BX250618.1|BX250618 BX250618 Pinus pinaster differenciat... 168 2e-050
gb|BX250070.1|BX250070 BX250070 Pinus pinaster differenciat... 148 2e-044
gb|BX252804.1|BX252804 BX252804 Pinus pinaster differenciat... 75 1e-032
>gb|CO161136.1|CO161136 FLD1_26_H05.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_26_H05_A029 5', mRNA sequence
Length = 820
Score = 222 bits (479), Expect(3) = 2e-066
Identities = 86/147 (58%), Positives = 117/147 (79%)
Frame = +1 / +2
Query: 346 DMSVVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIG 525
+ VVE FL+ G++ ++D+I++II MGFK+E++ + E EF +VQDADRLDAIG
Sbjct: 215 ECKVVERFLESHGVESVKRDKILSIINRMGFKDELAGVTTCEFVPEFGVVQDADRLDAIG 394
Query: 526 AIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQTSINHFHEKLFKLKDMMKT 705
AIGIARC T+GGS+N LHDP + R +L+KE+Y ++ KQT++NHFHEKL KLKD+MKT
Sbjct: 395 AIGIARCLTFGGSRNRVLHDPEVKARTNLTKEEYSKQDGKQTTMNHFHEKLLKLKDLMKT 574
Query: 706 EAGKRRAEKRHRFMEEFVAEFYEEWSG 786
EAG+RRAE+RH+FM++F+ EFYEEW G
Sbjct: 575 EAGRRRAERRHKFMQDFLTEFYEEWEG 655
Score = 119 bits (255), Expect = 3e-026
Identities = 68/150 (45%), Positives = 78/150 (52%)
Frame = -3 / -2
Query: 788 LPLHXXXXXXXXXXXNLCLFSALLFPASVFIMXXXXXXXXXXXLIDVCFSSLDMYFSFDK 609
LP H NLCL SA L PASVFI I VC S +Y SF K
Sbjct: 657 LPSHSS*NSVRKSCINLCLLSARLLPASVFIRSLSLRSFSWK*FIVVCLPSCLLYSSFVK 478
Query: 608 LSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVGSTIDLFETSFLNPI 429
R GSC+ FLLPP V+H AIPIAPIASRRSAS T NS S + +S L PI
Sbjct: 477 FVRALTSGSCSTRFLLPPKVRHRAIPIAPIASRRSAS*TTPNSGTNSQVVTPASSSLKPI 298
Query: 428 PLIIATISSFWPSSNPTS*RNISTTLISST 339
++ +I S + S P RN STTL S +
Sbjct: 297 LFMMLSILSLFTDSTPWLSRNRSTTLHSDS 208
Score = 39.6 bits (80), Expect(3) = 2e-066
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +1 / +2
Query: 265 PDRLLTVELAALLHDVGDYKYTKNNVE 345
P+ + VELA+LLHDVGD+KY + E
Sbjct: 137 PESMEIVELASLLHDVGDHKYASDESE 217
Score = 30.9 bits (61), Expect(3) = 2e-066
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = +1 / +2
Query: 175 GGRDASHDAAHALRVR 222
G DASHDA HALRVR
Sbjct: 50 GKWDASHDAFHALRVR 97
>gb|DR070788.1|DR070788 RTDK1_15_C12.g1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_15_C12_A029 5', mRNA sequence
Length = 838
Score = 221 bits (477), Expect(3) = 3e-066
Identities = 86/147 (58%), Positives = 117/147 (79%)
Frame = +1 / +3
Query: 346 DMSVVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIG 525
+ VVE FL+ G++ ++D+I++II MGFK+E++ + E EF +VQDADRLDAIG
Sbjct: 318 ECKVVERFLESHGVESVKRDKILSIINRMGFKDELAGVTTCEFVPEFGVVQDADRLDAIG 497
Query: 526 AIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQTSINHFHEKLFKLKDMMKT 705
AIGIARC T+GGS+N LHDP + R +L+KE+Y ++ KQT++NHFHEKL KLKD+MKT
Sbjct: 498 AIGIARCLTFGGSRNRVLHDPEVKARINLTKEEYSKQDGKQTTMNHFHEKLLKLKDLMKT 677
Query: 706 EAGKRRAEKRHRFMEEFVAEFYEEWSG 786
EAG+RRAE+RH+FM++F+ EFYEEW G
Sbjct: 678 EAGRRRAERRHKFMQDFLTEFYEEWEG 758
Score = 119 bits (255), Expect = 3e-026
Identities = 68/150 (45%), Positives = 78/150 (52%)
Frame = -3 / -1
Query: 788 LPLHXXXXXXXXXXXNLCLFSALLFPASVFIMXXXXXXXXXXXLIDVCFSSLDMYFSFDK 609
LP H NLCL SA L PASVFI I VC S +Y SF K
Sbjct: 760 LPSHSS*NSVRKSCINLCLLSARLLPASVFIRSLSLRSFSWK*FIVVCLPSCLLYSSFVK 581
Query: 608 LSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVGSTIDLFETSFLNPI 429
R GSC+ FLLPP V+H AIPIAPIASRRSAS T NS S + +S L PI
Sbjct: 580 FIRALTSGSCSTRFLLPPKVRHRAIPIAPIASRRSAS*TTPNSGTNSQVVTPASSSLKPI 401
Query: 428 PLIIATISSFWPSSNPTS*RNISTTLISST 339
++ +I S + S P RN STTL S +
Sbjct: 400 LFMMLSILSLFTDSTPWLSRNRSTTLHSDS 311
Score = 39.6 bits (80), Expect(3) = 3e-066
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +1 / +3
Query: 265 PDRLLTVELAALLHDVGDYKYTKNNVE 345
P+ + VELA+LLHDVGD+KY + E
Sbjct: 240 PESMEIVELASLLHDVGDHKYASDESE 320
Score = 30.9 bits (61), Expect(3) = 3e-066
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = +1 / +3
Query: 175 GGRDASHDAAHALRVR 222
G DASHDA HALRVR
Sbjct: 153 GKWDASHDAFHALRVR 200
>gb|DR070708.1|DR070708 RTDK1_15_C12.b1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_15_C12_A029 3', mRNA sequence
Length = 829
Score = 221 bits (477), Expect(2) = 1e-062
Identities = 86/147 (58%), Positives = 117/147 (79%)
Frame = +1 / +1
Query: 346 DMSVVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIG 525
+ VVE FL+ G++ ++D+I++II MGFK+E++ + E EF +VQDADRLDAIG
Sbjct: 121 ECKVVERFLESHGVESVKRDKILSIINRMGFKDELAGVTTCEFVPEFGVVQDADRLDAIG 300
Query: 526 AIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQTSINHFHEKLFKLKDMMKT 705
AIGIARC T+GGS+N LHDP + R +L+KE+Y ++ KQT++NHFHEKL KLKD+MKT
Sbjct: 301 AIGIARCLTFGGSRNRVLHDPEVKARINLTKEEYSKQDGKQTTMNHFHEKLLKLKDLMKT 480
Query: 706 EAGKRRAEKRHRFMEEFVAEFYEEWSG 786
EAG+RRAE+RH+FM++F+ EFYEEW G
Sbjct: 481 EAGRRRAERRHKFMQDFLTEFYEEWEG 561
Score = 119 bits (255), Expect = 3e-026
Identities = 68/150 (45%), Positives = 78/150 (52%)
Frame = -3 / -3
Query: 788 LPLHXXXXXXXXXXXNLCLFSALLFPASVFIMXXXXXXXXXXXLIDVCFSSLDMYFSFDK 609
LP H NLCL SA L PASVFI I VC S +Y SF K
Sbjct: 563 LPSHSS*NSVRKSCINLCLLSARLLPASVFIRSLSLRSFSWK*FIVVCLPSCLLYSSFVK 384
Query: 608 LSRGRILGSCNALFLLPPYVKHLAIPIAPIASRRSASCTIANSRVGSTIDLFETSFLNPI 429
R GSC+ FLLPP V+H AIPIAPIASRRSAS T NS S + +S L PI
Sbjct: 383 FIRALTSGSCSTRFLLPPKVRHRAIPIAPIASRRSAS*TTPNSGTNSQVVTPASSSLKPI 204
Query: 428 PLIIATISSFWPSSNPTS*RNISTTLISST 339
++ +I S + S P RN STTL S +
Sbjct: 203 LFMMLSILSLFTDSTPWLSRNRSTTLHSDS 114
Score = 39.6 bits (80), Expect(2) = 1e-062
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +1 / +1
Query: 265 PDRLLTVELAALLHDVGDYKYTKNNVE 345
P+ + VELA+LLHDVGD+KY + E
Sbjct: 43 PESMEIVELASLLHDVGDHKYASDESE 123
>gb|BX250618.1|BX250618 BX250618 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP039B11, mRNA sequence
Length = 675
Score = 168 bits (362), Expect(3) = 2e-050
Identities = 68/121 (56%), Positives = 93/121 (76%)
Frame = +1 / +3
Query: 346 DMSVVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIG 525
+ VVE FL+ G++ ++D+I++II MGFK+E++ + E EF +VQDADRLDAIG
Sbjct: 312 ECKVVERFLESHGVESVKRDKILSIINRMGFKDELAGVTTCEFVPEFGVVQDADRLDAIG 491
Query: 526 AIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQTSINHFHEKLFKLKDMMKT 705
AIGIARC T+GGS+N LHDP + R +L+KE+Y ++ KQT++NHFHEKL KLKD+MKT
Sbjct: 492 AIGIARCLTFGGSRNRVLHDPEVKARINLTKEEYXQQDGKQTTMNHFHEKLLKLKDLMKT 671
Query: 706 E 708
E
Sbjct: 672 E 674
Score = 39.6 bits (80), Expect(3) = 2e-050
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +1 / +3
Query: 265 PDRLLTVELAALLHDVGDYKYTKNNVE 345
P+ + VELA+LLHDVGD+KY + E
Sbjct: 234 PESMEIVELASLLHDVGDHKYASDESE 314
Score = 30.9 bits (61), Expect(3) = 2e-050
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = +1 / +3
Query: 175 GGRDASHDAAHALRVR 222
G DASHDA HALRVR
Sbjct: 147 GKWDASHDAFHALRVR 194
>gb|BX250070.1|BX250070 BX250070 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP032A11, mRNA sequence
Length = 674
Score = 148 bits (317), Expect(3) = 2e-044
Identities = 60/115 (52%), Positives = 84/115 (73%)
Frame = +1 / +3
Query: 346 DMSVVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIG 525
+ VVE FL+ G++ ++D+I++II MGFK+E++ + E EF +VQDADRLDAIG
Sbjct: 312 ECKVVERFLESHGVESVKRDKILSIINRMGFKDELAGVTTCEFVPEFGVVQDADRLDAIG 491
Query: 526 AIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQTSINHFHEKLFKLK 690
AIGIARC T+GGS+N LHDP + R +L+KE+Y ++ KQT++NHFH KL K
Sbjct: 492 AIGIARCLTFGGSRNRVLHDPEVKARINLTKEEYSKQDGKQTTMNHFHXKLLNSK 656
Score = 39.6 bits (80), Expect(3) = 2e-044
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +1 / +3
Query: 265 PDRLLTVELAALLHDVGDYKYTKNNVE 345
P+ + VELA+LLHDVGD+KY + E
Sbjct: 234 PESMEIVELASLLHDVGDHKYASDESE 314
Score = 30.9 bits (61), Expect(3) = 2e-044
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = +1 / +3
Query: 175 GGRDASHDAAHALRVR 222
G DASHDA HALRVR
Sbjct: 147 GKWDASHDAFHALRVR 194
>gb|BX252804.1|BX252804 BX252804 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP071A03, mRNA sequence
Length = 662
Score = 74.8 bits (157), Expect(4) = 1e-032
Identities = 32/63 (50%), Positives = 46/63 (73%)
Frame = +1 / +3
Query: 346 DMSVVEMFLQEVGLDEGQKDEIVAIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIG 525
+ VVE FL+ G++ ++D+I++II MGFK+E++ + E EF +VQDADRLDAIG
Sbjct: 312 ECKVVERFLESHGVESVKRDKILSIINRMGFKDELAGVTTCEFVPEFGVVQDADRLDAIG 491
Query: 526 AIG 534
AIG
Sbjct: 492 AIG 500
Score = 55.6 bits (115), Expect(4) = 1e-032
Identities = 18/26 (69%), Positives = 24/26 (92%)
Frame = +1 / +1
Query: 709 AGKRRAEKRHRFMEEFVAEFYEEWSG 786
AG+RRAE+RH+FM++F+ EFYEEW G
Sbjct: 499 AGRRRAERRHKFMQDFLTEFYEEWEG 576
Score = 39.6 bits (80), Expect(4) = 1e-032
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +1 / +3
Query: 265 PDRLLTVELAALLHDVGDYKYTKNNVE 345
P+ + VELA+LLHDVGD+KY + E
Sbjct: 234 PESMEIVELASLLHDVGDHKYASDESE 314
Score = 30.9 bits (61), Expect(4) = 1e-032
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = +1 / +3
Query: 175 GGRDASHDAAHALRVR 222
G DASHDA HALRVR
Sbjct: 147 GKWDASHDAFHALRVR 194
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 550,582,650
Number of Sequences: 355925
Number of extensions: 7879663
Number of successful extensions: 50807
Number of sequences better than 1.0e-020: 8
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 16818380080
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)