TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF41c07.yg.2.1
(691 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus adde... 213 9e-055
gb|BG625791.1|BG625791 NXPV_066_B06_F NXPV (Nsf Xylem Plani... 116 2e-025
gb|BG625796.1|BG625796 NXPV_066_B11_F NXPV (Nsf Xylem Plani... 116 2e-025
>gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_25_F10_A029 5', mRNA sequence
Length = 753
Score = 213 bits (460), Expect = 9e-055
Identities = 87/89 (97%), Positives = 89/89 (100%)
Frame = -1 / +1
Query: 268 LKVRMHYGHPDVFDRIFHITRGGISKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGK 89
LKVRMHYGHPDVFDRIFHITRGGISKASR+INISEDIYAGFNSTLRQGNITHHEYIQVGK
Sbjct: 7 LKVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGK 186
Query: 88 GRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
GRDVGLNQIA+FEGKVAGGNGEQVLSRDV
Sbjct: 187 GRDVGLNQIAMFEGKVAGGNGEQVLSRDV 273
Score = 152 bits (327), Expect = 2e-036
Identities = 69/89 (77%), Positives = 72/89 (80%)
Frame = +3 / -2
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILIT 182
TS L+TCSP PPATFPSN AI PTSRPFPT +YS CV+FP R VELNPA ISSLILI
Sbjct: 272 TSLLKTCSPFPPATFPSNIAI*LSPTSRPFPT*MYS*CVIFPCRNVELNPA*ISSLILII 93
Query: 183 RDALLMPPRVM*NIRSKTSGCP*CIRTLR 269
RDALLMPP VM* I SKTSG P*CIRT R
Sbjct: 92 RDALLMPPLVM*KILSKTSG*P*CIRTFR 6
>gb|BG625791.1|BG625791 NXPV_066_B06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_066_B06 5' similar to Arabidopsis
thaliana sequence At2g13675 callose synthase
(1,3-beta-glucan synthase) like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 401
Score = 116 bits (247), Expect = 2e-025
Identities = 45/55 (81%), Positives = 51/55 (92%)
Frame = -1 / +3
Query: 166 EDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
EDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+ FE KVA GNGEQ LSRD+
Sbjct: 3 EDIFAGFNSTLRKGNVTHHEYMQVGKGRDVGLNQISQFEAKVANGNGEQTLSRDI 167
>gb|BG625796.1|BG625796 NXPV_066_B11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_066_B11 5' similar to Arabidopsis
thaliana sequence At5g13000 callose synthase catalytic
subunit -like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 517
Score = 116 bits (247), Expect = 2e-025
Identities = 45/55 (81%), Positives = 51/55 (92%)
Frame = -1 / +3
Query: 166 EDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
EDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+ FE KVA GNGEQ LSRD+
Sbjct: 3 EDIFAGFNSTLRKGNVTHHEYMQVGKGRDVGLNQISQFEAKVANGNGEQTLSRDI 167
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 338,200,029
Number of Sequences: 355925
Number of extensions: 4767459
Number of successful extensions: 36318
Number of sequences better than 1.0e-020: 6
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 9543423165
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)