TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF1g01.yg.2.1
(335 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus adde... 191 1e-048
gb|BG625791.1|BG625791 NXPV_066_B06_F NXPV (Nsf Xylem Plani... 116 8e-026
gb|BG625796.1|BG625796 NXPV_066_B11_F NXPV (Nsf Xylem Plani... 116 8e-026
>gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_25_F10_A029 5', mRNA sequence
Length = 753
Score = 191 bits (412), Expect = 1e-048
Identities = 80/88 (90%), Positives = 82/88 (93%)
Frame = -2 / +1
Query: 265 KVRXXYXXPDVFDRIFXITRGGIXKASRVINISEDIYAGFNSTLRQGNITHHEYIQVGKG 86
KVR Y PDVFDRIF ITRGGI KASR+INISEDIYAGFNSTLRQGNITHHEYIQVGKG
Sbjct: 10 KVRMHYGHPDVFDRIFHITRGGISKASRIINISEDIYAGFNSTLRQGNITHHEYIQVGKG 189
Query: 85 RDVGLNQIALFEGKVAGGNGEQVLSRDV 2
RDVGLNQIA+FEGKVAGGNGEQVLSRDV
Sbjct: 190 RDVGLNQIAMFEGKVAGGNGEQVLSRDV 273
Score = 128 bits (274), Expect = 1e-029
Identities = 58/80 (72%), Positives = 61/80 (76%)
Frame = +3 / -2
Query: 3 TSRLRTCSPLPPATFPSNSAIWFKPTSRPFPTWIYSWCVMFPWRKVELNPAYISSLILIT 182
TS L+TCSP PPATFPSN AI PTSRPFPT +YS CV+FP R VELNPA ISSLILI
Sbjct: 272 TSLLKTCSPFPPATFPSNIAI*LSPTSRPFPT*MYS*CVIFPCRNVELNPA*ISSLILII 93
Query: 183 RDAXXMPPRVXXNIRSKTSG 242
RDA MPP V I SKTSG
Sbjct: 92 RDALLMPPLVM*KILSKTSG 33
>gb|BG625791.1|BG625791 NXPV_066_B06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_066_B06 5' similar to Arabidopsis
thaliana sequence At2g13675 callose synthase
(1,3-beta-glucan synthase) like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 401
Score = 116 bits (247), Expect = 8e-026
Identities = 45/55 (81%), Positives = 51/55 (92%)
Frame = -2 / +3
Query: 166 EDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
EDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+ FE KVA GNGEQ LSRD+
Sbjct: 3 EDIFAGFNSTLRKGNVTHHEYMQVGKGRDVGLNQISQFEAKVANGNGEQTLSRDI 167
>gb|BG625796.1|BG625796 NXPV_066_B11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_066_B11 5' similar to Arabidopsis
thaliana sequence At5g13000 callose synthase catalytic
subunit -like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 517
Score = 116 bits (247), Expect = 8e-026
Identities = 45/55 (81%), Positives = 51/55 (92%)
Frame = -2 / +3
Query: 166 EDIYAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDV 2
EDI+AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+ FE KVA GNGEQ LSRD+
Sbjct: 3 EDIFAGFNSTLRKGNVTHHEYMQVGKGRDVGLNQISQFEAKVANGNGEQTLSRDI 167
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 132,611,680
Number of Sequences: 355925
Number of extensions: 1657113
Number of successful extensions: 75120
Number of sequences better than 1.0e-020: 6
length of database: 72,425,745
effective HSP length: 49
effective length of database: 54,985,420
effective search space used: 3409096040
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)