TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF18e02.yg.3.1
(315 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE123544.1|BE123544 NXNV_149_E06_F Nsf Xylem Normal wood... 31 0.51
gb|BE582336.1|BE582336 NXCI_032_B11_F NXCI (Nsf Xylem Compr... 31 0.54
gb|BE607130.1|BE607130 NXCI_037_B02_F NXCI (Nsf Xylem Compr... 31 0.59
gb|CV034327.1|CV034327 RTNACL1_40_D11.b1_A029 Roots plus ad... 33 0.64
gb|CO157736.1|CO157736 FLD1_1_F01.g1_A029 Root flooded Pinu... 31 0.68
gb|DR023592.1|DR023592 STRS1_58_F01.g1_A034 Shoot tip pitch... 31 0.98
gb|CF476159.1|CF476159 RTWW2_21_C12.g1_A021 Well-watered lo... 31 1.1
gb|DR743734.1|DR743734 RTCU1_17_G08.g1_A029 Roots plus adde... 31 1.2
gb|CO200491.1|CO200491 GEO2_7_F11.g1_A032 Root gravitropism... 31 1.2
gb|CO369654.1|CO369654 RTK1_48_C09.g1_A029 Roots minus pota... 31 2.3
gb|BE996822.1|BE996822 NXCI_101_G05_F NXCI (Nsf Xylem Compr... 29 2.8
gb|CF395829.1|CF395829 RTDS2_17_A01.g1_A021 Drought-stresse... 30 4.3
gb|DR072540.1|DR072540 RTDK1_27_B02.b1_A029 Roots, dark Pin... 30 4.3
gb|DR100940.1|DR100940 STRR1_69_H12.b1_A033 Stem Response R... 30 4.3
gb|BQ700839.1|BQ700839 NXRV112_B03_F NXRV (Nsf Xylem Root w... 30 5.9
gb|CF388070.1|CF388070 RTDR2_1_G09.b1_A021 Loblolly pine ro... 23 6.8
gb|CX649386.1|CX649386 COLD1_35_A05.b1_A029 Root cold Pinus... 23 7.9
gb|CF400979.1|CF400979 RTWW1_9_E06.b1_A015 Well-watered lob... 23 8.0
gb|CR394418.1|CR394418 CR394418 RN Pinus pinaster cDNA clon... 29 8.1
gb|CX713279.1|CX713279 RTPQ1_8_D01.b1_A032 Roots treated wi... 29 8.1
gb|CX713355.1|CX713355 RTPQ1_8_D01.g1_A032 Roots treated wi... 29 8.1
gb|DR692573.1|DR692573 EST1082661 Normalized pine embryo li... 29 8.1
gb|DR693850.1|DR693850 EST1083940 Normalized pine embryo li... 29 8.1
gb|DT637726.1|DT637726 EST1152657 Normalized pine embryo li... 29 8.1
gb|CF391186.1|CF391186 RTDR3_4_E01.b1_A022 Loblolly pine ro... 23 9.0
gb|CF472001.1|CF472001 RTDS1_8_A06.b1_A015 Drought-stressed... 23 9.2
>gb|BE123544.1|BE123544 NXNV_149_E06_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_149_E06 5' similar to Arabidopsis thaliana
sequence At3g52125 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 318
Score = 31.3 bits (62), Expect(2) = 0.51
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -3
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 223 CCGWWLSGAVTRGTSY 176
Score = 17.6 bits (32), Expect(2) = 0.51
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -2
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 188 RNKLHELIDLTILNLHHQHHRVQM 117
>gb|BE582336.1|BE582336 NXCI_032_B11_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_032_B11 5' similar to Arabidopsis
thaliana sequence At3g52125 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 337
Score = 31.3 bits (62), Expect(2) = 0.54
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -3
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 173 CCGWWLSGAVTRGTSY 126
Score = 17.6 bits (32), Expect(2) = 0.54
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -2
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 138 RNKLHELIDLTILNLHHQHHRVQM 67
>gb|BE607130.1|BE607130 NXCI_037_B02_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_037_B02 5' similar to Arabidopsis
thaliana sequence At3g52125 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 368
Score = 31.3 bits (62), Expect(2) = 0.59
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -1
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 173 CCGWWLSGAVTRGTSY 126
Score = 17.6 bits (32), Expect(2) = 0.59
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -3
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 138 RNKLHELIDLTILNLHHQHHRVQM 67
>gb|CV034327.1|CV034327 RTNACL1_40_D11.b1_A029 Roots plus added NaCl Pinus taeda cDNA clone
RTNACL1_40_D11_A029 3', mRNA sequence
Length = 742
Score = 33.1 bits (66), Expect = 0.64
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +3 / +3
Query: 174 VTVRTIQKDAEEKKASFNPRP 236
V++RT+ +D +++KA FNP P
Sbjct: 273 VSIRTVDRDTDQRKAWFNPTP 335
>gb|CO157736.1|CO157736 FLD1_1_F01.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_1_F01_A029 5', mRNA sequence
Length = 422
Score = 31.3 bits (62), Expect(2) = 0.68
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -1
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 200 CCGWWLSGAVTRGTSY 153
Score = 17.6 bits (32), Expect(2) = 0.68
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -3
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 165 RNKLHELIDLTILNLHHQHHRVQM 94
>gb|DR023592.1|DR023592 STRS1_58_F01.g1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_58_F01_A034 5', mRNA sequence
Length = 613
Score = 31.3 bits (62), Expect(2) = 0.98
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -3
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 395 CCGWWLSGAVTRGTSY 348
Score = 17.6 bits (32), Expect(2) = 0.98
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -2
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 360 RNKLHELIDLTILNLHHQHHRVQM 289
>gb|CF476159.1|CF476159 RTWW2_21_C12.g1_A021 Well-watered loblolly pine roots WW2 Pinus
taeda cDNA clone RTWW2_21_C12_A021 5', mRNA sequence
Length = 728
Score = 31.3 bits (62), Expect(2) = 1.1
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -3
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 354 CCGWWLSGAVTRGTSY 307
Score = 17.6 bits (32), Expect(2) = 1.1
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -2
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 319 RNKLHELIDLTILNLHHQHHRVQM 248
>gb|DR743734.1|DR743734 RTCU1_17_G08.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_17_G08_A029 5', mRNA sequence
Length = 752
Score = 31.3 bits (62), Expect(2) = 1.2
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -2
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 238 CCGWWLSGAVTRGTSY 191
Score = 17.6 bits (32), Expect(2) = 1.2
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -1
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 203 RNKLHELIDLTILNLHHQHHRVQM 132
>gb|CO200491.1|CO200491 GEO2_7_F11.g1_A032 Root gravitropism October 2003 test Pinus taeda
cDNA clone GEO2_7_F11_A032 5', mRNA sequence
Length = 756
Score = 31.3 bits (62), Expect(2) = 1.2
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -2
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 419 CCGWWLSGAVTRGTSY 372
Score = 17.6 bits (32), Expect(2) = 1.2
Identities = 8/24 (33%), Positives = 12/24 (50%)
Frame = +3 / -1
Query: 243 RLXINWLYDLTTTDGHHDGSNFQV 314
R ++ L DLT + HH Q+
Sbjct: 384 RNKLHELIDLTILNLHHQHHRVQM 313
>gb|CO369654.1|CO369654 RTK1_48_C09.g1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_48_C09_A029 5', mRNA sequence
Length = 770
Score = 31.3 bits (62), Expect = 2.3
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +2 / -1
Query: 17 CCSWWIISAITTATPY 64
CC WW+ A+T T Y
Sbjct: 395 CCGWWLSGAVTRGTSY 348
>gb|BE996822.1|BE996822 NXCI_101_G05_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_101_G05 5' similar to Arabidopsis
thaliana sequence At3g52125 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 392
Score = 28.6 bits (56), Expect(2) = 2.8
Identities = 7/14 (50%), Positives = 9/14 (64%)
Frame = +2 / -1
Query: 17 CCSWWIISAITTAT 58
CC WW+ A+T T
Sbjct: 173 CCGWWLSGAVTRGT 132
Score = 18.0 bits (33), Expect(2) = 2.8
Identities = 7/17 (41%), Positives = 8/17 (47%)
Frame = +3 / -3
Query: 261 LYDLTTTDGHHDGSNFQ 311
L DLT + HH Q
Sbjct: 120 LIDLTILNXHHQHHRVQ 70
>gb|CF395829.1|CF395829 RTDS2_17_A01.g1_A021 Drought-stressed loblolly pine roots DS2 Pinus
taeda cDNA clone RTDS2_17_A01_A021 5', mRNA sequence
Length = 628
Score = 30.4 bits (60), Expect = 4.3
Identities = 9/19 (47%), Positives = 16/19 (84%)
Frame = +3 / -2
Query: 174 VTVRTIQKDAEEKKASFNP 230
V++RT+ +D +++KA FNP
Sbjct: 531 VSIRTVDRDTDQRKAWFNP 475
>gb|DR072540.1|DR072540 RTDK1_27_B02.b1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_27_B02_A029 3', mRNA sequence
Length = 618
Score = 30.4 bits (60), Expect = 4.3
Identities = 9/19 (47%), Positives = 16/19 (84%)
Frame = +3 / +2
Query: 174 VTVRTIQKDAEEKKASFNP 230
V++RT+ +D +++KA FNP
Sbjct: 113 VSIRTVDRDTDQRKAWFNP 169
>gb|DR100940.1|DR100940 STRR1_69_H12.b1_A033 Stem Response Resistant Pinus taeda cDNA clone
STRR1_69_H12_A033 3', mRNA sequence
Length = 848
Score = 30.4 bits (60), Expect = 4.3
Identities = 9/19 (47%), Positives = 16/19 (84%)
Frame = +3 / -2
Query: 174 VTVRTIQKDAEEKKASFNP 230
V++RT+ +D +++KA FNP
Sbjct: 520 VSIRTVDRDTDQRKAWFNP 464
>gb|BQ700839.1|BQ700839 NXRV112_B03_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV112_B03 5', mRNA sequence
Length = 723
Score = 29.9 bits (59), Expect = 5.9
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1 / +2
Query: 55 NPIFHIFPLIHMQSSWLWFSST 120
NPI H+ +I +WL+F+ T
Sbjct: 50 NPIIHVEEIIEKSKNWLYFNDT 115
>gb|CF388070.1|CF388070 RTDR2_1_G09.b1_A021 Loblolly pine roots recovering from drought
DR2 Pinus taeda cDNA clone RTDR2_1_G09_A021 3', mRNA
sequence
Length = 394
Score = 23.1 bits (44), Expect(2) = 6.8
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = +2 / +1
Query: 17 CCSWW 31
CCSWW
Sbjct: 40 CCSWW 54
Score = 22.1 bits (42), Expect(2) = 6.8
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2 / +3
Query: 164 DYFCYCEDHSER 199
DY CYC ER
Sbjct: 270 DYVCYCSGCCER 305
>gb|CX649386.1|CX649386 COLD1_35_A05.b1_A029 Root cold Pinus taeda cDNA clone
COLD1_35_A05_A029 3', mRNA sequence
Length = 459
Score = 23.1 bits (44), Expect(2) = 7.9
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = +2 / +3
Query: 17 CCSWW 31
CCSWW
Sbjct: 114 CCSWW 128
Score = 22.1 bits (42), Expect(2) = 7.9
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2 / +2
Query: 164 DYFCYCEDHSER 199
DY CYC ER
Sbjct: 344 DYVCYCSGCCER 379
>gb|CF400979.1|CF400979 RTWW1_9_E06.b1_A015 Well-watered loblolly pine roots WW1 Pinus
taeda cDNA clone RTWW1_9_E06_A015 3', mRNA sequence
Length = 460
Score = 23.1 bits (44), Expect(2) = 8.0
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = +2 / +3
Query: 17 CCSWW 31
CCSWW
Sbjct: 54 CCSWW 68
Score = 22.1 bits (42), Expect(2) = 8.0
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2 / +2
Query: 164 DYFCYCEDHSER 199
DY CYC ER
Sbjct: 284 DYVCYCSGCCER 319
>gb|CR394418.1|CR394418 CR394418 RN Pinus pinaster cDNA clone RN78H04, mRNA sequence
Length = 651
Score = 29.5 bits (58), Expect = 8.1
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2 / -3
Query: 179 SNRNNLGKYTAFIWCYLH*R 120
S NNL K +F+WC +H R
Sbjct: 271 SQNNNLKKLHSFLWCSIHCR 212
>gb|CX713279.1|CX713279 RTPQ1_8_D01.b1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_8_D01_A032 3', mRNA sequence
Length = 821
Score = 29.5 bits (58), Expect = 8.1
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -2 / -1
Query: 164 LGKYTAFIWCYLH*RVLENHNHELCI 87
LG + F+WC LH ++ H +CI
Sbjct: 245 LGAQSQFLWCLLHIMLILQHIELVCI 168
>gb|CX713355.1|CX713355 RTPQ1_8_D01.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_8_D01_A032 5', mRNA sequence
Length = 793
Score = 29.5 bits (58), Expect = 8.1
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -2 / -1
Query: 164 LGKYTAFIWCYLH*RVLENHNHELCI 87
LG + F+WC LH ++ H +CI
Sbjct: 376 LGAQSQFLWCLLHIMLILQHIELVCI 299
>gb|DR692573.1|DR692573 EST1082661 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWADN74 3' end, mRNA sequence
Length = 785
Score = 29.5 bits (58), Expect = 8.1
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2 / -3
Query: 179 SNRNNLGKYTAFIWCYLH*R 120
S NNL K +F+WC +H R
Sbjct: 426 SQNNNLKKLHSFLWCSIHCR 367
>gb|DR693850.1|DR693850 EST1083940 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE502 3' end, mRNA sequence
Length = 740
Score = 29.5 bits (58), Expect = 8.1
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2 / -1
Query: 179 SNRNNLGKYTAFIWCYLH*R 120
S NNL K +F+WC +H R
Sbjct: 182 SQNNNLKKLHSFLWCSIHCR 123
>gb|DT637726.1|DT637726 EST1152657 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMH685 3' end, mRNA sequence
Length = 707
Score = 29.5 bits (58), Expect = 8.1
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2 / -1
Query: 179 SNRNNLGKYTAFIWCYLH*R 120
S NNL K +F+WC +H R
Sbjct: 434 SQNNNLKKLHSFLWCSIHCR 375
>gb|CF391186.1|CF391186 RTDR3_4_E01.b1_A022 Loblolly pine roots recovering from drought
DR3 Pinus taeda cDNA clone RTDR3_4_E01_A022 3', mRNA
sequence
Length = 528
Score = 23.1 bits (44), Expect(2) = 9.0
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = +2 / +1
Query: 17 CCSWW 31
CCSWW
Sbjct: 58 CCSWW 72
Score = 22.1 bits (42), Expect(2) = 9.0
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2 / +3
Query: 164 DYFCYCEDHSER 199
DY CYC ER
Sbjct: 288 DYVCYCSGCCER 323
>gb|CF472001.1|CF472001 RTDS1_8_A06.b1_A015 Drought-stressed loblolly pine roots DS1 Pinus
taeda cDNA clone RTDS1_8_A06_A015 3', mRNA sequence
Length = 539
Score = 23.1 bits (44), Expect(2) = 9.2
Identities = 5/5 (100%), Positives = 5/5 (100%)
Frame = +2 / +3
Query: 17 CCSWW 31
CCSWW
Sbjct: 120 CCSWW 134
Score = 22.1 bits (42), Expect(2) = 9.2
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +2 / +2
Query: 164 DYFCYCEDHSER 199
DY CYC ER
Sbjct: 350 DYVCYCSGCCER 385
Database: Pinus_nucl_with_EST.fasta
Posted date: May 2, 2006 3:25 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 143,838,213
Number of Sequences: 355925
Number of extensions: 1553072
Number of successful extensions: 87637
Number of sequences better than 10.0: 33
length of database: 72,425,745
effective HSP length: 49
effective length of database: 54,985,420
effective search space used: 3024198100
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)