TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAF13b04.yg.2.1
         (370 letters)

Database: Pinus_nucl_with_EST.fasta 
           355,925 sequences; 217,277,237 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DR388038.1|DR388038  RTHG1_25_F10.g1_A029 Roots plus adde...   154   2e-043
gb|BQ696956.1|BQ696956  NXPV_047_C11_F NXPV (Nsf Xylem Plani...   153   4e-037

>gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus added mercury Pinus taeda cDNA
           clone RTHG1_25_F10_A029 5', mRNA sequence
          Length = 753

 Score =  154 bits (330), Expect(2) = 2e-043
 Identities = 61/75 (81%), Positives = 66/75 (88%)
 Frame = +3 / +1

                                                                       
Query: 126 TQFLFQIGVFTAVPMILGFILESGVLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAIL 305
           TQFLFQIG+FTAVPMI+GFILE G L A V F TMQ Q+CS+FFTFSLGTRTHYFGR IL
Sbjct: 475 TQFLFQIGIFTAVPMIMGFILEQGFLKAIVSFFTMQLQLCSIFFTFSLGTRTHYFGRTIL 654

                          
Query: 306 HGGAKYRATGRGFVV 350
           HGGA Y+ATGRGFVV
Sbjct: 655 HGGAXYKATGRGFVV 699




 Score =  117 bits (251), Expect(2) = 7e-031
 Identities = 54/82 (65%), Positives = 58/82 (70%)
 Frame = -1 / -2

                                                                       
Query: 349 TTKPLPVALYFAPPCNIARPK*WVLVPSEKVKNTEHIWNCIVIN*TKAVKTPDSRMKPRI 170
           TTKPLPVALY APP  I RPK* VLVP E VKN EH  +CIV   T A K P S+MKP I
Sbjct: 698 TTKPLPVALYXAPP*RIVRPK**VLVPRENVKNIEHSCSCIVKKLTMAFKNPCSKMKPII 519

                                 
Query: 169 MGTAVNTPIWKRN*VFRAAFKA 104
           +GTAVN PIW +N V RAA  A
Sbjct: 518 IGTAVNIPIWNKNCVLRAAVSA 453




 Score = 40.5 bits (82), Expect(2) = 2e-043
 Identities = 16/22 (72%), Positives = 18/22 (81%)
 Frame = +3 / +1

                                 
Query: 18  YIFLYGKMYLALSGVGESIQNR 83
           YIFLYG+ YLALSGVG SI  +
Sbjct: 373 YIFLYGRAYLALSGVGGSIMEK 438




 Score = 35.0 bits (70), Expect(2) = 7e-031
 Identities = 13/19 (68%), Positives = 14/19 (73%)
 Frame = -1 / -2

                              
Query: 73  IDSPTPDKARYIFP*RNMY 17
           ID PTPDKARY  P R +Y
Sbjct: 428 IDPPTPDKARYALPYRKIY 372



>gb|BQ696956.1|BQ696956 NXPV_047_C11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_047_C11 5' similar to Arabidopsis
           thaliana sequence At5g13000 callose synthase catalytic
           subunit -like protein see
           http://mips.gsf.de/proj/thal/db/index.html, mRNA
           sequence
          Length = 601

 Score =  153 bits (329), Expect = 4e-037
 Identities = 63/111 (56%), Positives = 80/111 (72%)
 Frame = +3 / +2

                                                                       
Query: 18  YIFLYGKMYLALSGVGESIQNRADIXXXXXXXXXXXTQFLFQIGVFTAVPMILGFILESG 197
           Y+FLYG++YL LSG+ +S+ N+AD+           +Q L QIG+  A+PM++   LE G
Sbjct: 38  YVFLYGRLYLVLSGLEKSLVNQADMRKNFPLEAVLASQSLVQIGLLMALPMVMEIGLERG 217

                                                              
Query: 198 VLTAFVQFITMQFQMCSVFFTFSLGTRTHYFGRAILHGGAKYRATGRGFVV 350
             +A   F+ MQ Q+ SVFFTFSLGT+THYFGR ILHGGAKYRATGRGFVV
Sbjct: 218 FRSALSDFVIMQLQLASVFFTFSLGTKTHYFGRTILHGGAKYRATGRGFVV 370



  Database: Pinus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:45 PM
  Number of letters in database: 217,277,237
  Number of sequences in database:  355,925
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 224,656,220
Number of Sequences: 355925
Number of extensions: 3337647
Number of successful extensions: 113476
Number of sequences better than 1.0e-020: 4
length of database: 72,425,745
effective HSP length: 50
effective length of database: 54,629,495
effective search space used: 3933323640
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)