TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE40a10.yg.2.1
(709 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO159016.1|CO159016 FLD1_10_F07.g1_A029 Root flooded Pin... 281 9e-081
gb|BG317521.1|BG317521 NXPV_002_G06_F NXPV (Nsf Xylem Plani... 241 4e-063
gb|BQ696956.1|BQ696956 NXPV_047_C11_F NXPV (Nsf Xylem Plani... 225 3e-058
gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus adde... 196 2e-049
gb|DR071679.1|DR071679 RTDK1_21_A05.g1_A029 Roots, dark Pin... 144 5e-040
gb|AL750522.1|AL750522 AL750522 RN Pinus pinaster cDNA clon... 127 4e-036
gb|CD025387.1|CD025387 NXSI_050_E11_F NXSI (Nsf Xylem Side ... 112 2e-024
gb|DR117586.1|DR117586 RTMG1_7_G10.g1_A029 Roots minus magn... 78 6e-024
>gb|CO159016.1|CO159016 FLD1_10_F07.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_10_F07_A029 5', mRNA sequence
Length = 796
Score = 281 bits (607), Expect(2) = 9e-081
Identities = 106/166 (63%), Positives = 128/166 (77%)
Frame = -1 / +3
Query: 586 LXLGLMKAVFSFITMQLQFCSVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKF 407
L G KA+ F+ MQLQ +VFFTFSLGT+THY+GRT+LHGGAKYR TGRGFVV H KF
Sbjct: 3 LERGFRKALSDFVIMQLQMAAVFFTFSLGTKTHYYGRTLLHGGAKYRGTGRGFVVFHAKF 182
Query: 406 AENYRLYSRSHFVKALEVALLLIVYIAYGYTKGGSSSFILITISSWFLVMSWLFAPYIFN 227
AENYR YSRSHFVK LE+ LLLIVY Y + +ILIT S WF+V +WLFAP++FN
Sbjct: 183 AENYRFYSRSHFVKGLELMLLLIVYTVYSQAHRKTVPYILITYSMWFMVGAWLFAPFLFN 362
Query: 226 PSGFEWQKTVEDFDDWTNWLLYKGGVGVKGDNSWESWWDEEQAHIQ 89
PSGFEWQK V+D+ DW W+ +GG+GV + SWESWWDE+Q H++
Sbjct: 363 PSGFEWQKIVDDWTDWNKWINNRGGIGVPHEKSWESWWDEDQDHLK 500
Score = 87.2 bits (184), Expect(2) = 4e-030
Identities = 45/81 (55%), Positives = 50/81 (61%)
Frame = +3 / -1
Query: 336 TIRRRATSSALTK*LRE*SL*FSANFMCRTTKPLPVARYFAPP*RIVLPK*CVLVPSEKV 515
TI+ SS LTK LRE* *FSANF +TT P PV YFAPP* V P *CV VP EKV
Sbjct: 253 TIKSSINSSPLTKWLRE*KR*FSANFA*KTTNPRPVPLYFAPP*SNVRP**CVFVPREKV 74
Query: 516 KNTEQNWSCIVIKLKTAFISP 578
K T SC++ K A +P
Sbjct: 73 KKTAAICSCMITKSLKALRNP 11
Score = 78.0 bits (164), Expect(3) = 1e-026
Identities = 29/80 (36%), Positives = 57/80 (71%)
Frame = +2 / -3
Query: 314 CVSICNVNDQEESNLKRFDKVTSRIEPIIFSKLYVPNNKTSSSSTILCTAMKDCSTKVMC 493
C++I ++++QE+ L+ FDKV + IE ++F KL + +NK++ S+++ C++MK C+ +MC
Sbjct: 275 CLAIDSIHNQEQHQLESFDKVAA*IEAVVFCKLRMKDNKSTPSTSVFCSSMK*CAAIIMC 96
Query: 494 PCSKRKGEKHRTELELHCNK 553
KR+ +++ L+LH +K
Sbjct: 95 LRPKRESKENCCHLQLHDHK 36
Score = 64.3 bits (134), Expect(2) = 4e-030
Identities = 26/61 (42%), Positives = 42/61 (68%)
Frame = +2 / -3
Query: 89 LDMRLLLIPPRFPTVVTLYTNSTFIK*PVCPVIKVLNSLLPLETRRVENVRREKPGHNEE 268
L+M L+LIPP FPT+ +TN++ + P+ P+ V+++ LPLE+RR+E R + P N +
Sbjct: 500 LEMVLILIPPGFPTLFMRHTNTSTVVDPLVPIRPVIHNFLPLESRRIEQERSK*PCPNHK 321
Query: 269 P 271
P
Sbjct: 320 P 318
Score = 46.0 bits (94), Expect(3) = 1e-026
Identities = 21/38 (55%), Positives = 25/38 (65%)
Frame = +3 / -1
Query: 180 QSSKSSTVFCHSKPEGLKM*GAKSQDITRNQLLIVIRI 293
QS +SST+FCHS PEGL GA S T N + VI+I
Sbjct: 409 QSVQSSTIFCHSNPEGLNRKGANSHAPTINHME*VIKI 296
Score = 40.0 bits (81), Expect(2) = 9e-081
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = -1 / +3
Query: 112 DEEQAHIQTFRGRILETILSLRFLMFQYGIVY 17
D++ R RI E +LS RFL++QYG+VY
Sbjct: 483 DQDHLKHAGLRARIFEILLSFRFLLYQYGLVY 578
Score = 33.6 bits (67), Expect(3) = 1e-026
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +1 / -2
Query: 100 PAPHPTKIPNCCHPLHQ 150
P PHPT+IPN H HQ
Sbjct: 489 PDPHPTRIPNSFHAAHQ 439
>gb|BG317521.1|BG317521 NXPV_002_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_002_G06 5' similar to Arabidopsis
thaliana sequence At5g13000 callose synthase catalytic
subunit -like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 421
Score = 241 bits (521), Expect = 4e-063
Identities = 91/140 (65%), Positives = 108/140 (77%)
Frame = -1 / +1
Query: 523 VFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHFVKALEVALL 344
VFFTFSLGT+THY+GRT+LHGGAKYRATGRGFVV H KFA+NYR YSRSHFVK LE+ LL
Sbjct: 1 VFFTFSLGTKTHYYGRTLLHGGAKYRATGRGFVVFHAKFADNYRFYSRSHFVKGLELMLL 180
Query: 343 LIVYIAYGYTKGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTNWLL 164
LIVY YG + +++LIT S WF+V +WLFAP++FNPSGFEWQK V+D+ DW W
Sbjct: 181 LIVYNVYGQPYRNTIAYLLITFSMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKWXN 360
Query: 163 YKGGVGVKGDNSWESWWDEE 104
G +GV E WWD+E
Sbjct: 361 SHGXIGVPXXKXXEXWWDDE 420
Score = 76.7 bits (161), Expect(2) = 1e-021
Identities = 40/67 (59%), Positives = 43/67 (64%)
Frame = +3 / -3
Query: 318 YPYAM*TIRRRATSSALTK*LRE*SL*FSANFMCRTTKPLPVARYFAPP*RIVLPK*CVL 497
+P + TI SS LTK*LRE* * SAN +TTKP PVA YFAPP V P *CV
Sbjct: 206 WP*TLYTINSNINSSPLTK*LRE*KR*LSANLA*KTTKPRPVALYFAPPWSNVRP**CVF 27
Query: 498 VPSEKVK 518
VP EKVK
Sbjct: 26 VPREKVK 6
Score = 46.4 bits (95), Expect(2) = 1e-021
Identities = 21/37 (56%), Positives = 25/37 (67%)
Frame = +3 / -3
Query: 180 QSSKSSTVFCHSKPEGLKM*GAKSQDITRNQLLIVIR 290
QS +SST+FCHS PEGL GAKS T N + VI+
Sbjct: 344 QSVQSSTIFCHSNPEGLNRKGAKSHVPTINHMEKVIK 234
>gb|BQ696956.1|BQ696956 NXPV_047_C11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_047_C11 5' similar to Arabidopsis
thaliana sequence At5g13000 callose synthase catalytic
subunit -like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 601
Score = 225 bits (485), Expect = 3e-058
Identities = 93/150 (62%), Positives = 111/150 (74%)
Frame = -1 / +2
Query: 664 LDAALNAQFLXXIGIXTXVPMIMGFILXLGLMKAVFSFITMQLQFCSVFFTFSLGTRTHY 485
L+A L +Q L IG+ +PM+M L G A+ F+ MQLQ SVFFTFSLGT+THY
Sbjct: 128 LEAVLASQSLVQIGLLMALPMVMEIGLERGFRSALSDFVIMQLQLASVFFTFSLGTKTHY 307
Query: 484 FGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHFVKALEVALLLIVYIAYGYTKGG 305
FGRTILHGGAKYRATGRGFVVRH K+AENYRLYSRSHF K LE+ +LL VY Y
Sbjct: 308 FGRTILHGGAKYRATGRGFVVRHEKYAENYRLYSRSHFTKGLELMMLLFVYNIYCSAAKD 487
Query: 304 SSSFILITISSWFLVMSWLFAPYIFNPSGF 215
S++LIT+S WFLV++WLFAP++FNP F
Sbjct: 488 FVSYLLITLSMWFLVVTWLFAPFLFNPXXF 577
>gb|DR388038.1|DR388038 RTHG1_25_F10.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_25_F10_A029 5', mRNA sequence
Length = 753
Score = 196 bits (422), Expect = 2e-049
Identities = 80/99 (80%), Positives = 86/99 (86%)
Frame = -1 / +1
Query: 664 LDAALNAQFLXXIGIXTXVPMIMGFILXLGLMKAVFSFITMQLQFCSVFFTFSLGTRTHY 485
L AALN QFL IGI T VPMIMGFIL G +KA+ SF TMQLQ CS+FFTFSLGTRTHY
Sbjct: 457 LTAALNTQFLFQIGIFTAVPMIMGFILEQGFLKAIVSFFTMQLQLCSIFFTFSLGTRTHY 636
Query: 484 FGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHFV 368
FGRTILHGGA Y+ATGRGFVV+HIKFA+NYRLYSRSHFV
Sbjct: 637 FGRTILHGGAXYKATGRGFVVQHIKFADNYRLYSRSHFV 753
Score = 112 bits (240), Expect = 2e-024
Identities = 58/99 (58%), Positives = 63/99 (63%)
Frame = +3 / -2
Query: 369 TK*LRE*SL*FSANFMCRTTKPLPVARYFAPP*RIVLPK*CVLVPSEKVKNTEQNWSCIV 548
TK LRE*SL* SAN +C TTKPLPVA Y APP*RIV PK* VLVP E VKN E + SCIV
Sbjct: 752 TKWLRE*SL*LSANLICWTTKPLPVALYXAPP*RIVRPK**VLVPRENVKNIEHSCSCIV 573
Query: 549 IKLKTAFISPSXRMKPXXXXXXXXXXXXXKN*AFKAASS 665
KL AF +P +MKP KN +AA S
Sbjct: 572 KKLTMAFKNPCSKMKPIIIGTAVNIPIWNKNCVLRAAVS 456
Score = 105 bits (223), Expect = 5e-022
Identities = 44/95 (46%), Positives = 62/95 (65%)
Frame = +2 / -1
Query: 371 KVTSRIEPIIFSKLYVPNNKTSSSSTILCTAMKDCSTKVMCPCSKRKGEKHRTELELHCN 550
++ +R+EP+I K + +NKTSSS +LC MK+ + KV+ PCS+RK EK+R L LHC
Sbjct: 750 EMAARVEPVIIRKFDMLDNKTSSSCFVLCATMKNSAAKVVSPCSQRKCEKYRA*L*LHCE 571
Query: 551 KTKNSLHQPQLXNETHYHRDXXXNTNLXQKLSIQS 655
+T N L + L +ETHYH TNL KL ++S
Sbjct: 570 ETNNGLQESLLQDETHYHWHSGKYTNLE*KLCVKS 466
>gb|DR071679.1|DR071679 RTDK1_21_A05.g1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_21_A05_A029 5', mRNA sequence
Length = 607
Score = 144 bits (310), Expect(2) = 5e-040
Identities = 51/91 (56%), Positives = 67/91 (73%)
Frame = -1 / +1
Query: 361 LEVALLLIVYIAYGYTKGGSSSFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDD 182
LE+ LLLIVY Y + +ILIT S WF+V +WLFAP++FNPSGFEWQK V+D+ D
Sbjct: 4 LELMLLLIVYTVYSQAHRKTVPYILITYSMWFMVGAWLFAPFLFNPSGFEWQKIVDDWTD 183
Query: 181 WTNWLLYKGGVGVKGDNSWESWWDEEQAHIQ 89
W W+ +GG+GV + SWESWWDE+Q H++
Sbjct: 184 WNKWINNRGGIGVPHEKSWESWWDEDQDHLK 276
Score = 40.0 bits (81), Expect(2) = 5e-040
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = -1 / +1
Query: 112 DEEQAHIQTFRGRILETILSLRFLMFQYGIVY 17
D++ R RI E +LS RFL++QYG+VY
Sbjct: 259 DQDHLKHAGLRARIFEILLSFRFLLYQYGLVY 354
>gb|AL750522.1|AL750522 AL750522 RN Pinus pinaster cDNA clone RN03D12 similar to GLUCAN
(CALLOSE) SYNTHASE, mRNA sequence
Length = 378
Score = 127 bits (273), Expect(2) = 4e-036
Identities = 41/70 (58%), Positives = 55/70 (78%)
Frame = -1 / +2
Query: 298 SFILITISSWFLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTNWLLYKGGVGVKGDNSWES 119
+++LIT WF+V +WLFAP++FNPSGFEWQK V+D+ DW W+ GG+GV D SWES
Sbjct: 11 AYLLITFCMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKWINSHGGIGVPQDKSWES 190
Query: 118 WWDEEQAHIQ 89
WWD+EQ H++
Sbjct: 191 WWDDEQEHLK 220
Score = 43.7 bits (89), Expect(2) = 4e-036
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = -1 / +2
Query: 112 DEEQAHIQTFRGRILETILSLRFLMFQYGIVY 17
++E RGRI E +LSLRF ++QYGIVY
Sbjct: 203 EQEHLKYSGLRGRIWEILLSLRFFLYQYGIVY 298
>gb|CD025387.1|CD025387 NXSI_050_E11_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_050_E11 5' similar to Arabidopsis thaliana
sequence At2g36850 putative glucan synthase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 163
Score = 112 bits (240), Expect = 2e-024
Identities = 40/54 (74%), Positives = 44/54 (81%)
Frame = -1 / +1
Query: 196 EDFDDWTNWLLYKGGVGVKGDNSWESWWDEEQAHIQTFRGRILETILSLRFLMF 35
+DFDDWT WLLYKGGVGVK + SWE+WWDEEQ HI+T RGRI ETI LRF F
Sbjct: 1 QDFDDWTTWLLYKGGVGVKAEESWETWWDEEQVHIRTLRGRIFETIXXLRFXXF 162
>gb|DR117586.1|DR117586 RTMG1_7_G10.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_7_G10_A029 5', mRNA sequence
Length = 763
Score = 78.0 bits (164), Expect(3) = 6e-024
Identities = 27/39 (69%), Positives = 31/39 (79%)
Frame = -1 / +3
Query: 268 FLVMSWLFAPYIFNPSGFEWQKTVEDFDDWTNWLLYKGG 152
FLV SW AP+IFNPSGF+W K V DFDD+ NW+ YKGG
Sbjct: 3 FLVFSWTMAPFIFNPSGFDWLKAVYDFDDFMNWIWYKGG 119
Score = 37.7 bits (76), Expect(3) = 6e-024
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = -1 / +3
Query: 112 DEEQAHIQTFRGRILETILSLRFLMFQYGIVY 17
+ + H G++LE ++ +RFL QYGIVY
Sbjct: 156 EHDHLHSTGIWGKVLEILIDIRFLFLQYGIVY 251
Score = 32.7 bits (65), Expect(3) = 6e-024
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = -1 / +3
Query: 148 GVKGDNSWESWWDEEQAHIQT 86
G K D WE WW EE H+ +
Sbjct: 114 GGKADQCWEVWWYEEHDHLHS 176
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 362,496,281
Number of Sequences: 355925
Number of extensions: 5330339
Number of successful extensions: 48804
Number of sequences better than 1.0e-020: 12
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 9866929035
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)