TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MAD56_a5f4.3.2
(1520 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT635292.1|DT635292 EST1150223 Normalized pine embryo li... 51 3e-028
gb|CO173165.1|CO173165 NDL1_34_A09.g1_A029 Needles control ... 51 4e-028
gb|DR694912.1|DR694912 EST1085005 Normalized pine embryo li... 51 4e-028
gb|DR078481.1|DR078481 RTFEPL1_5_A10.b1_A029 Roots plus add... 73 7e-024
>gb|DT635292.1|DT635292 EST1150223 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMGF28 3' end, mRNA sequence
Length = 828
Score = 51.5 bits (106), Expect(5) = 3e-028
Identities = 21/40 (52%), Positives = 25/40 (62%)
Frame = +3 / +1
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTP 389
VP +F+FGDS VDVGNNN+L PYG DF + P
Sbjct: 172 VPALFIFGDSTVDVGNNNYLFTLVKSNFPPYGRDFDTHNP 291
Score = 42.8 bits (87), Expect(5) = 3e-028
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +3 / +1
Query: 732 MGEQDAPAYISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGSRVPMANG 890
M + P ++ ++ + LY+LGARR+G+ +P +GC P + +G
Sbjct: 634 MRQFTVPQFVEFLMQKFSAFTKGLYQLGARRVGVTSLPPLGCLPAAITLFGHG 792
Score = 38.2 bits (77), Expect(5) = 3e-028
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +3 / +1
Query: 399 SGRFTNGYNLADLVARRLGFKMSPPAYLS 485
+GRF++G D VA LGF PPAYLS
Sbjct: 292 TGRFSDGRLATDYVAETLGFTTFPPAYLS 378
Score = 37.3 bits (75), Expect(5) = 3e-028
Identities = 14/31 (45%), Positives = 23/31 (74%)
Frame = +3 / +1
Query: 618 KTKARMSWGRCKLSSMVSRSLFLISAGGNDF 710
++K S GR SS+VS++L+++SAG +DF
Sbjct: 511 QSKVERSVGRANASSIVSKALYVVSAGASDF 603
Score = 28.6 bits (56), Expect(5) = 3e-028
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +2 / +3
Query: 524 NWCQLRVWWIWH 559
NW +LR W IWH
Sbjct: 405 NWGELRFWCIWH 440
>gb|CO173165.1|CO173165 NDL1_34_A09.g1_A029 Needles control Pinus taeda cDNA clone
NDL1_34_A09_A029 5', mRNA sequence
Length = 838
Score = 51.5 bits (106), Expect(5) = 4e-028
Identities = 21/40 (52%), Positives = 25/40 (62%)
Frame = +3 / +1
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTP 389
VP +F+FGDS VDVGNNN+L PYG DF + P
Sbjct: 94 VPALFIFGDSTVDVGNNNYLFTLVKSNFPPYGRDFDTHNP 213
Score = 42.3 bits (86), Expect(5) = 4e-028
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +3 / +1
Query: 732 MGEQDAPAYISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGSRVPMANG 890
M + P ++ ++ + LY+LGARR+G+ +P +GC P + +G
Sbjct: 556 MRQFTVPQFVEFLMQEFSVFTKGLYQLGARRVGVTSLPPLGCLPAAITLFGHG 714
Score = 38.2 bits (77), Expect(5) = 4e-028
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +3 / +1
Query: 399 SGRFTNGYNLADLVARRLGFKMSPPAYLS 485
+GRF++G D VA LGF PPAYLS
Sbjct: 214 TGRFSDGRLATDYVAETLGFTSFPPAYLS 300
Score = 37.3 bits (75), Expect(5) = 4e-028
Identities = 14/31 (45%), Positives = 23/31 (74%)
Frame = +3 / +1
Query: 618 KTKARMSWGRCKLSSMVSRSLFLISAGGNDF 710
++K S GR SS+VS++L+++SAG +DF
Sbjct: 433 QSKVERSVGRANASSIVSKALYVVSAGASDF 525
Score = 28.6 bits (56), Expect(5) = 4e-028
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +2 / +3
Query: 524 NWCQLRVWWIWH 559
NW +LR W IWH
Sbjct: 327 NWGELRFWCIWH 362
>gb|DR694912.1|DR694912 EST1085005 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAEI32 3' end, mRNA sequence
Length = 749
Score = 51.5 bits (106), Expect(5) = 4e-028
Identities = 21/40 (52%), Positives = 25/40 (62%)
Frame = +3 / +1
Query: 270 VPLMFVFGDSLVDVGNNNFLPPPAPRAASPYGIDFPSGTP 389
VP +F+FGDS VDVGNNN+L PYG DF + P
Sbjct: 139 VPALFIFGDSTVDVGNNNYLFTLVKSNFPPYGRDFDTHNP 258
Score = 42.3 bits (86), Expect(5) = 4e-028
Identities = 14/46 (30%), Positives = 26/46 (56%)
Frame = +3 / +1
Query: 732 MGEQDAPAYISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGS 869
M + P ++ ++ + LY+LGARR+G+ +P +GC P +
Sbjct: 601 MRQFTVPQFVEFLMQKFSAFTKGLYQLGARRVGVTSLPPLGCLPAA 738
Score = 38.2 bits (77), Expect(5) = 4e-028
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +3 / +1
Query: 399 SGRFTNGYNLADLVARRLGFKMSPPAYLS 485
+GRF++G D VA LGF PPAYLS
Sbjct: 259 TGRFSDGRLATDYVAETLGFTTFPPAYLS 345
Score = 37.3 bits (75), Expect(5) = 4e-028
Identities = 14/31 (45%), Positives = 23/31 (74%)
Frame = +3 / +1
Query: 618 KTKARMSWGRCKLSSMVSRSLFLISAGGNDF 710
++K S GR SS+VS++L+++SAG +DF
Sbjct: 478 QSKVERSVGRANASSIVSKALYVVSAGASDF 570
Score = 28.6 bits (56), Expect(5) = 4e-028
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +2 / +3
Query: 524 NWCQLRVWWIWH 559
NW +LR W IWH
Sbjct: 372 NWGELRFWCIWH 407
>gb|DR078481.1|DR078481 RTFEPL1_5_A10.b1_A029 Roots plus added iron Pinus taeda cDNA clone
RTFEPL1_5_A10_A029 3', mRNA sequence
Length = 809
Score = 72.5 bits (152), Expect(3) = 7e-024
Identities = 27/79 (34%), Positives = 43/79 (54%)
Frame = +3 / -3
Query: 960 AVASSMPGMRYSIASTYNFVTDLMDSHLVAGLRVVDRACCGSGKLNAAVMCAQPNTTYCS 1139
++A ++PGM+ + Y + + + G RACCG+G + AV+C + C+
Sbjct: 477 SLAKTLPGMKMVVFDIYTSLYNFVQHPSDNGFAEARRACCGTGVIETAVLCNPRSIGTCA 298
Query: 1140 DRDDYMFWDMLHPTQATNE 1196
+ Y+FWD HPTQA NE
Sbjct: 297 NATQYVFWDSFHPTQAANE 241
Score = 42.8 bits (87), Expect(3) = 7e-024
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +3 / -3
Query: 732 MGEQDAPAYISSMVSTYVQHIDALYKLGARRLGILDVPAIGCTPGSRVPMANG 890
M + P ++ ++ + LY+LGARR+G+ +P +GC P + +G
Sbjct: 708 MRQFTVPQFVEFLMQKFSAFTKGLYQLGARRVGVTSLPPLGCLPAAITLFGHG 550
Score = 34.1 bits (68), Expect(3) = 7e-024
Identities = 12/23 (52%), Positives = 19/23 (82%)
Frame = +3 / -3
Query: 642 GRCKLSSMVSRSLFLISAGGNDF 710
GR SS+VS++L+++SAG +DF
Sbjct: 807 GRANASSIVSKALYVVSAGASDF 739
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 902,190,357
Number of Sequences: 355925
Number of extensions: 15000530
Number of successful extensions: 85452
Number of sequences better than 1.0e-020: 4
length of database: 72,425,745
effective HSP length: 54
effective length of database: 53,205,795
effective search space used: 24049019340
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)