TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8228901.3.1
(1173 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF476969.1|CF476969 RTWW3_4_C07.g1_A022 Well-watered lob... 58 3e-028
gb|DR691692.1|DR691692 EST1081779 Normalized pine embryo li... 59 1e-027
gb|CF665310.1|CF665310 RTCNT1_15_F01.b1_A029 Root control P... 56 4e-023
>gb|CF476969.1|CF476969 RTWW3_4_C07.g1_A022 Well-watered loblolly pine roots WW3 Pinus
taeda cDNA clone RTWW3_4_C07_A022 5', mRNA sequence
Length = 554
Score = 57.9 bits (120), Expect(4) = 3e-028
Identities = 21/55 (38%), Positives = 34/55 (61%)
Frame = +2 / +1
Query: 425 SIARNHKGEAAEALPPCYISGLPGSFYTRIFPSESVHLFHSLFSVHWHSQASQQL 589
SI +H+ E + +G+PGSFY R+FP +++H HS +S+HW SQ ++
Sbjct: 385 SINTDHQNEEGAVDRWYFAAGIPGSFYGRLFPDKTLHFVHSSYSLHWLSQVPPEI 549
Score = 48.7 bits (100), Expect(4) = 3e-028
Identities = 21/57 (36%), Positives = 33/57 (57%)
Frame = +2 / +1
Query: 146 IQEKVMFQIKPVLEEATRAAYSALLPQTMVVADLGCSSGPNTLRFISEVIGIIARHC 316
IQ+ V+ ++P+LEEA + + +T +ADLGCSSGPN L + + + C
Sbjct: 118 IQKNVLQIVQPMLEEAILSMKLTVDCKTFCIADLGCSSGPNALFAVENITKTLKAKC 288
Score = 37.7 bits (76), Expect(4) = 3e-028
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +2 / +1
Query: 344 PQLQFFLNDLPGNDFNNLFLLIQQFN 421
PQ F NDLP +DFN+LF ++ F+
Sbjct: 310 PQCHVFFNDLPASDFNSLFRILPPFS 387
Score = 36.8 bits (74), Expect(4) = 3e-028
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = +2 / +1
Query: 77 MTIERDLHMATGNGETSYTKNSRIQEKVMFQI 172
+ +E+D HM +G GE+SY +NS +K + QI
Sbjct: 46 LKMEKDFHMNSGLGESSYAQNSTFIQKNVLQI 141
>gb|DR691692.1|DR691692 EST1081779 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWADB65 3' end, mRNA sequence
Length = 874
Score = 58.8 bits (122), Expect(4) = 1e-027
Identities = 20/57 (35%), Positives = 35/57 (61%)
Frame = +2 / +3
Query: 425 SIARNHKGEAAEALPPCYISGLPGSFYTRIFPSESVHLFHSLFSVHWHSQASQQLKD 595
S+ +++ E + +G+PGSFY R+FP +++H HS +S+HW SQ ++ D
Sbjct: 399 SVNTDYQNEEGAVDRSYFATGVPGSFYGRLFPDDTLHFVHSSYSIHWLSQVPAEILD 569
Score = 44.6 bits (91), Expect(4) = 1e-027
Identities = 18/49 (36%), Positives = 28/49 (57%)
Frame = +2 / +1
Query: 170 IKPVLEEATRAAYSALLPQTMVVADLGCSSGPNTLRFISEVIGIIARHC 316
++P+LEEA + + +T +ADLGCSSGPN L + + + C
Sbjct: 157 VQPMLEEAILSMNLTVECKTFCIADLGCSSGPNALFAVENITKTLKAKC 303
Score = 39.6 bits (80), Expect(4) = 1e-027
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +2 / +3
Query: 335 HDYPQLQFFLNDLPGNDFNNLF 400
H PQ Q F NDLP +DFN+LF
Sbjct: 315 HTVPQCQVFFNDLPASDFNSLF 380
Score = 35.9 bits (72), Expect(4) = 1e-027
Identities = 14/32 (43%), Positives = 21/32 (65%)
Frame = +2 / +1
Query: 77 MTIERDLHMATGNGETSYTKNSRIQEKVMFQI 172
+ +E+D HM G GE+SY +NS + +K QI
Sbjct: 61 LKMEKDFHMNAGLGESSYAQNSALFQKSGLQI 156
>gb|CF665310.1|CF665310 RTCNT1_15_F01.b1_A029 Root control Pinus taeda cDNA clone
RTCNT1_15_F01_A029 3', mRNA sequence
Length = 674
Score = 55.6 bits (115), Expect(3) = 4e-023
Identities = 18/33 (54%), Positives = 26/33 (78%)
Frame = +2 / -1
Query: 476 YISGLPGSFYTRIFPSESVHLFHSLFSVHWHSQ 574
+ +G+PGSFY R+FP +S+ FHS +S+HW SQ
Sbjct: 158 FAAGVPGSFYGRLFPDKSIQFFHSSYSLHWLSQ 60
Score = 54.2 bits (112), Expect(3) = 4e-023
Identities = 24/76 (31%), Positives = 40/76 (52%)
Frame = +2 / -1
Query: 77 MTIERDLHMATGNGETSYTKNSRIQEKVMFQIKPVLEEATRAAYSALLPQTMVVADLGCS 256
M +E+ HM G G++SY +NS + +K + L E + + + +A+LGCS
Sbjct: 530 MEMEKMFHMNAGLGDSSYAQNSTMLQKRALNLGEPLLEESILSMKMAKCERFCIAELGCS 351
Query: 257 SGPNTLRFISEVIGII 304
SGPN L +I ++
Sbjct: 350 SGPNALLVTENIIRVL 303
Score = 36.8 bits (74), Expect(3) = 4e-023
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +2 / -1
Query: 344 PQLQFFLNDLPGNDFNNLF 400
PQ Q F NDLP DFN+LF
Sbjct: 269 PQFQVFFNDLPATDFNSLF 213
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 681,833,475
Number of Sequences: 355925
Number of extensions: 10396354
Number of successful extensions: 57094
Number of sequences better than 1.0e-020: 3
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 18050299640
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)