TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5705822.3.1
(888 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR745937.1|DR745937 RTCU1_33_C10.g1_A029 Roots plus adde... 310 7e-084
gb|DR745862.1|DR745862 RTCU1_33_C10.b1_A029 Roots plus adde... 256 1e-067
gb|CX648461.1|CX648461 COLD1_28_F12.g1_A029 Root cold Pinus... 243 2e-063
>gb|DR745937.1|DR745937 RTCU1_33_C10.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_33_C10_A029 5', mRNA sequence
Length = 744
Score = 310 bits (672), Expect = 7e-084
Identities = 119/178 (66%), Positives = 149/178 (83%)
Frame = +3 / -1
Query: 3 DVGWLKTVDQYYVGSNNSIQGACVQNVLDSLIPALLKDENRKFIYVEQAFFQRWWRNQND 182
DVGWLKTVDQYYVGSN+SIQ A V+ +LDS I AL +D NRKFIYVE AFF+RWWR Q++
Sbjct: 534 DVGWLKTVDQYYVGSNSSIQVAAVEYILDSFINALQEDPNRKFIYVEMAFFKRWWRQQSE 355
Query: 183 MIKDTVKGLISSGRLELINGGMCMHDEAAVHYIDMIDQTTLGHKYIKEEFGQIPRIGWQI 362
++ V+ L+ SG+LE INGG CMHDEA+ +Y+DMIDQTTLGH++IK+EFG++PR+GWQI
Sbjct: 354 EKREIVRRLVESGQLEFINGGWCMHDEASTYYVDMIDQTTLGHRFIKKEFGKVPRVGWQI 175
Query: 363 DPFGHSAVQAYLLGAEVGFDAFYFFRIDYQDRDTRKGTKELEVVWRGSKTLGSSADIF 536
DPFGHSAVQ YLL AEVGF+A +F R DYQD R+ + +E++WRGSK++GSSA IF
Sbjct: 174 DPFGHSAVQGYLLSAEVGFEALFFARADYQDIAKRREDRTMEMIWRGSKSVGSSAQIF 1
Score = 185 bits (398), Expect = 4e-046
Identities = 90/141 (63%), Positives = 99/141 (70%)
Frame = -3 / +2
Query: 424 ASNPTSAPRR*A*TAECPNGSICQPIRGIWPNSSLMYLCPSVV*SIMSI*CTAASSCMHM 245
ASNPTSA RR* *TAECP GSIC P RG PNS L+ CP VV*SIMS * ASSCMH
Sbjct: 113 ASNPTSALRR*P*TAECPKGSICHPTRGTLPNSFLINRCPRVV*SIMST**VDASSCMHH 292
Query: 244 PPFINSSRPELMSPFTVSLIMSFWFLHHLWKNACST*MNFRFSSLRSAGMSESSTFCTHA 65
PPFINSS P+ S T+SL S LHHL K A ST*+N RF S A ++ES + T A
Sbjct: 293 PPFINSSWPDSTSLLTISLFSSLCCLHHLLKKAIST*INLRFGSSCKALINESRMYSTAA 472
Query: 64 PWMELLDPT*YWSTVLSHPTS 2
+ELL+PT*Y STVLSHPTS
Sbjct: 473 TCIELLEPT*Y*STVLSHPTS 535
>gb|DR745862.1|DR745862 RTCU1_33_C10.b1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_33_C10_A029 3', mRNA sequence
Length = 715
Score = 256 bits (554), Expect = 1e-067
Identities = 98/138 (71%), Positives = 119/138 (86%)
Frame = +3 / -2
Query: 3 DVGWLKTVDQYYVGSNNSIQGACVQNVLDSLIPALLKDENRKFIYVEQAFFQRWWRNQND 182
DVGWLKTVDQYYVGSN+SIQ A V+ +LDS I AL +D NRKFIYVE AFF+RWWR Q++
Sbjct: 414 DVGWLKTVDQYYVGSNSSIQVAAVEYILDSFINALQEDPNRKFIYVEMAFFKRWWRQQSE 235
Query: 183 MIKDTVKGLISSGRLELINGGMCMHDEAAVHYIDMIDQTTLGHKYIKEEFGQIPRIGWQI 362
++ V+ L+ SG+LE INGG CMHDEA+ +Y+DMIDQTTLGH++IK+EFG++PR+GWQI
Sbjct: 234 EKREIVRRLVESGQLEFINGGWCMHDEASTYYVDMIDQTTLGHRFIKKEFGKVPRVGWQI 55
Query: 363 DPFGHSAVQAYLLGAEVG 416
DPFGHSAVQ YLL AEVG
Sbjct: 54 DPFGHSAVQGYLLSAEVG 1
Score = 178 bits (384), Expect = 4e-044
Identities = 87/138 (63%), Positives = 96/138 (69%)
Frame = -3 / +2
Query: 415 PTSAPRR*A*TAECPNGSICQPIRGIWPNSSLMYLCPSVV*SIMSI*CTAASSCMHMPPF 236
PTSA RR* *TAECP GSIC P RG PNS L+ CP VV*SIMS * ASSCMH PPF
Sbjct: 2 PTSALRR*P*TAECPKGSICHPTRGTLPNSFLINRCPRVV*SIMST**VDASSCMHHPPF 181
Query: 235 INSSRPELMSPFTVSLIMSFWFLHHLWKNACST*MNFRFSSLRSAGMSESSTFCTHAPWM 56
INSS P+ S T+SL S LHHL K A ST*+N RF S A ++ES + T A +
Sbjct: 182 INSSWPDSTSLLTISLFSSLCCLHHLLKKAIST*INLRFGSSCKALINESRMYSTAATCI 361
Query: 55 ELLDPT*YWSTVLSHPTS 2
ELL+PT*Y STVLSHPTS
Sbjct: 362 ELLEPT*Y*STVLSHPTS 415
>gb|CX648461.1|CX648461 COLD1_28_F12.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_28_F12_A029 5', mRNA sequence
Length = 832
Score = 243 bits (524), Expect = 2e-063
Identities = 88/140 (62%), Positives = 115/140 (82%)
Frame = +3 / +1
Query: 150 FFQRWWRNQNDMIKDTVKGLISSGRLELINGGMCMHDEAAVHYIDMIDQTTLGHKYIKEE 329
FFQRWWR Q++ ++ V+ L+ SG+LE INGG CMHDEA+ +Y+DMIDQTTLGH++IK+E
Sbjct: 409 FFQRWWRQQSEEKREIVRRLVESGQLEFINGGWCMHDEASTYYVDMIDQTTLGHRFIKKE 588
Query: 330 FGQIPRIGWQIDPFGHSAVQAYLLGAEVGFDAFYFFRIDYQDRDTRKGTKELEVVWRGSK 509
FG++PR+GWQIDPFGHSAVQ YLL AEVGF+A +F R DYQD R+ + +E++WRGSK
Sbjct: 589 FGKVPRVGWQIDPFGHSAVQGYLLSAEVGFEALFFARADYQDIAKRREDRTMEMIWRGSK 768
Query: 510 TLGSSADIFAGIFPKNYEPP 569
+LGSSA +F GI +Y+PP
Sbjct: 769 SLGSSAQVFTGILAHHYDPP 828
Score = 126 bits (269), Expect(2) = 1e-040
Identities = 59/88 (67%), Positives = 62/88 (70%)
Frame = -3 / -3
Query: 424 ASNPTSAPRR*A*TAECPNGSICQPIRGIWPNSSLMYLCPSVV*SIMSI*CTAASSCMHM 245
ASNPTSA RR* *TAECP GSIC P RG PNS L+ CP VV*SIMS * ASSCMH
Sbjct: 683 ASNPTSALRR*P*TAECPKGSICHPTRGTLPNSFLINRCPRVV*SIMST**VDASSCMHH 504
Query: 244 PPFINSSRPELMSPFTVSLIMSFWFLHH 161
PPFINSS P+ S T+SL S LHH
Sbjct: 503 PPFINSSWPDSTSLLTISLFSSLCCLHH 420
Score = 97.3 bits (206), Expect(2) = 8e-022
Identities = 44/77 (57%), Positives = 53/77 (68%)
Frame = +3 / +2
Query: 3 DVGWLKTVDQYYVGSNNSIQGACVQNVLDSLIPALLKDENRKFIYVEQAFFQRWWRNQND 182
DVGWLKTVDQYYVGSN+SIQ A V+ +LDS I AL +D NRKFIYVE AFF N+
Sbjct: 263 DVGWLKTVDQYYVGSNSSIQVAAVEYILDSFINALQEDPNRKFIYVEMAFFNGGGGNKVK 442
Query: 183 MIKDTVKGLISSGRLEL 233
++GL++ L L
Sbjct: 443 RKGKLLEGLLNLANLNL 493
Score = 61.1 bits (127), Expect(2) = 1e-040
Identities = 30/51 (58%), Positives = 36/51 (70%)
Frame = -3 / -2
Query: 154 KNACST*MNFRFSSLRSAGMSESSTFCTHAPWMELLDPT*YWSTVLSHPTS 2
K A ST*+N RF S A ++ES + T A +ELL+PT*Y STVLSHPTS
Sbjct: 414 KKAIST*INLRFGSSCKALINESRMYSTAATCIELLEPT*Y*STVLSHPTS 262
Score = 26.7 bits (52), Expect(2) = 8e-022
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +1 / +2
Query: 250 ACTMRRLYTILT*SIRLHLDTSTSRRSLAR 339
AC M+ L T* I+ L T SR+SLA+
Sbjct: 509 ACMMKHLPITWT**IKQPLGTDLSRKSLAK 598
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 524,040,905
Number of Sequences: 355925
Number of extensions: 8207406
Number of successful extensions: 60244
Number of sequences better than 1.0e-020: 3
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 12961936240
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)