TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4790360.2.1
(667 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR694977.1|DR694977 EST1085070 Normalized pine embryo li... 173 1e-042
gb|DT638989.1|DT638989 EST1153920 Normalized pine embryo li... 173 1e-042
gb|DR109699.1|DR109699 RTS1_4_C10.b1_A029 Roots minus sulfu... 171 4e-042
gb|BQ696389.1|BQ696389 NXPV_040_F05_F NXPV (Nsf Xylem Plani... 160 6e-039
gb|DR693981.1|DR693981 EST1084071 Normalized pine embryo li... 139 2e-032
gb|CX715693.1|CX715693 RTPQ1_35_H08.g1_A032 Roots treated w... 132 2e-030
gb|BI397792.1|BI397792 NXPV_107_E10_F NXPV (Nsf Xylem Plani... 107 9e-023
>gb|DR694977.1|DR694977 EST1085070 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAEJ07 3' end, mRNA sequence
Length = 793
Score = 173 bits (372), Expect = 1e-042
Identities = 65/109 (59%), Positives = 80/109 (73%)
Frame = +2 / +1
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EP LLGS+YF+HAYGPIY RN+S+RMFSNEDVTIG+WMLAMNVNH
Sbjct: 271 YEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGAWMLAMNVNH 450
Query: 182 ENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKMLELHERKECTGGPTV 328
E+ LC P+CT +SIAVWDIPKCSGLC+P ++ ELH++ C PT+
Sbjct: 451 EDNRELCSPECTPTSIAVWDIPKCSGLCNPAQRLRELHKKYVCMKSPTL 597
Score = 113 bits (242), Expect = 1e-024
Identities = 58/113 (51%), Positives = 69/113 (61%)
Frame = -2 / -3
Query: 339 TSAATVGPPVHXXXXXXXXXXXSGWHRPEHFGMSQTAIEDSVQSGSQSACVFSWLTFIAS 160
+S VG + +G RPEHFG+S TAIE V SG S+ + SWLTFIAS
Sbjct: 608 SSGGNVGLFIQTYFLWSSLSLCAGLQRPEHFGISHTAIEVGVHSGEHSSRLSSWLTFIAS 429
Query: 159 IHDPIVTSSLLNIRKLLFRNATKLATTSADKA*IGPYA*RKYCDPSKKDWGSW 1
IH P+VTSSLLNI L F + K AT SA++A IGP A* KY PSK GS+
Sbjct: 428 IHAPMVTSSLLNIL*LSFLSPDKPATISAERAYIGP*A*MKYSLPSKSPKGSY 270
>gb|DT638989.1|DT638989 EST1153920 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMHL79 3' end, mRNA sequence
Length = 850
Score = 173 bits (372), Expect = 1e-042
Identities = 65/109 (59%), Positives = 80/109 (73%)
Frame = +2 / +3
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EP LLGS+YF+HAYGPIY RN+S+RMFSNEDVTIG+WMLAMNVNH
Sbjct: 279 YEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGAWMLAMNVNH 458
Query: 182 ENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKMLELHERKECTGGPTV 328
E+ LC P+CT +SIAVWDIPKCSGLC+P ++ ELH++ C PT+
Sbjct: 459 EDNRELCSPECTPTSIAVWDIPKCSGLCNPAQRLRELHKKYVCMKSPTL 605
Score = 113 bits (242), Expect = 1e-024
Identities = 58/113 (51%), Positives = 69/113 (61%)
Frame = -2 / -1
Query: 339 TSAATVGPPVHXXXXXXXXXXXSGWHRPEHFGMSQTAIEDSVQSGSQSACVFSWLTFIAS 160
+S VG + +G RPEHFG+S TAIE V SG S+ + SWLTFIAS
Sbjct: 616 SSGGNVGLFIQTYFLWSSLSLCAGLQRPEHFGISHTAIEVGVHSGEHSSRLSSWLTFIAS 437
Query: 159 IHDPIVTSSLLNIRKLLFRNATKLATTSADKA*IGPYA*RKYCDPSKKDWGSW 1
IH P+VTSSLLNI L F + K AT SA++A IGP A* KY PSK GS+
Sbjct: 436 IHAPMVTSSLLNIL*LSFLSPDKPATISAERAYIGP*A*MKYSLPSKSPKGSY 278
>gb|DR109699.1|DR109699 RTS1_4_C10.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_4_C10_A029 3', mRNA sequence
Length = 808
Score = 171 bits (368), Expect = 4e-042
Identities = 65/112 (58%), Positives = 78/112 (69%)
Frame = +2 / -2
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EP ++LLGS+YF HAYGPIY RN+SFRM SNEDVTIG+WMLAMNV H
Sbjct: 633 YEPAAYLLGSEYFYHAYGPIYALSAEVVAGLAVARNDSFRMLSNEDVTIGAWMLAMNVEH 454
Query: 182 ENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKMLELHERKECTGGPTVAAE 337
E+ LC P C SIAVWDIPKCSGLC+P K+ ELH+ C+ PT+ A+
Sbjct: 453 EDNRELCHPHCKPDSIAVWDIPKCSGLCNPAEKLPELHKNNMCSTSPTLPAD 298
Score = 108 bits (230), Expect = 5e-023
Identities = 57/113 (50%), Positives = 68/113 (60%)
Frame = -2 / +2
Query: 339 TSAATVGPPVHXXXXXXXXXXXSGWHRPEHFGMSQTAIEDSVQSGSQSACVFSWLTFIAS 160
+SA +VG H +G RPEH G+S TAIE +Q G ++ + S TFIAS
Sbjct: 296 SSAGSVGLVEHILFLCNSGSFSAGLQRPEHLGISHTAIESGLQWG*HNSRLSSCSTFIAS 475
Query: 159 IHDPIVTSSLLNIRKLLFRNATKLATTSADKA*IGPYA*RKYCDPSKKDWGSW 1
IH P+VTSSLLNI KL F ATTSA+ A IGPYA* KY PSK GS+
Sbjct: 476 IHAPMVTSSLLNILKLSFLATANPATTSAESAYIGPYA**KYSLPSKYAAGSY 634
>gb|BQ696389.1|BQ696389 NXPV_040_F05_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_040_F05 5' similar to Arabidopsis
thaliana sequence At3g14960 galactosyltransferase,
putative see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 456
Score = 160 bits (345), Expect = 6e-039
Identities = 61/103 (59%), Positives = 75/103 (72%)
Frame = +2 / +1
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EP LLGS+YF+HAYGPIY RN+S+RMFSNEDVTIG+WMLAMNVNH
Sbjct: 31 YEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGAWMLAMNVNH 210
Query: 182 ENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKMLELHERKEC 310
E+ LC P+CT +SIAVWDIPKCS L +P ++ ELH++ C
Sbjct: 211 EDNRELCSPECTPTSIAVWDIPKCSXLXNPAQRLXELHKKYVC 339
Score = 105 bits (225), Expect = 2e-022
Identities = 53/90 (58%), Positives = 61/90 (67%)
Frame = -2 / -2
Query: 270 GWHRPEHFGMSQTAIEDSVQSGSQSACVFSWLTFIASIHDPIVTSSLLNIRKLLFRNATK 91
G EHFG+S TAIE V SG S+ + SWLTFIASIH P+VTSSLLNI L F + K
Sbjct: 299 GXQXXEHFGISHTAIEVGVHSGEHSSRLSSWLTFIASIHAPMVTSSLLNIL*LSFLSPDK 120
Query: 90 LATTSADKA*IGPYA*RKYCDPSKKDWGSW 1
AT SA++A IGP A* KY PSK GS+
Sbjct: 119 PATISAERAYIGP*A*MKYSLPSKSPKGSY 30
>gb|DR693981.1|DR693981 EST1084071 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAE664 3' end, mRNA sequence
Length = 817
Score = 139 bits (298), Expect = 2e-032
Identities = 53/85 (62%), Positives = 60/85 (70%)
Frame = +2 / +3
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EP ++LLGS+YF HAYGPIY RN+SFRM SNEDVTIG+WMLAMNV H
Sbjct: 495 YEPAAYLLGSEYFYHAYGPIYALSAEVVAGLAVARNDSFRMLSNEDVTIGAWMLAMNVEH 674
Query: 182 ENTHALCEPDCTESSIAVWDIPKCS 256
E+ LC P C SIAVWDIPKCS
Sbjct: 675 EDNRELCHPHCKPDSIAVWDIPKCS 749
>gb|CX715693.1|CX715693 RTPQ1_35_H08.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_35_H08_A032 5', mRNA sequence
Length = 649
Score = 132 bits (283), Expect = 2e-030
Identities = 50/78 (64%), Positives = 59/78 (75%)
Frame = +2 / -3
Query: 104 RNNSFRMFSNEDVTIGSWMLAMNVNHENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKM 283
RN+SFRM SNEDVTIG+WMLAMNV HE+ LC P C SIAVWDIPKCSGLC+P K+
Sbjct: 626 RNDSFRMLSNEDVTIGAWMLAMNVEHEDNRELCHPHCKPDSIAVWDIPKCSGLCNPAEKL 447
Query: 284 LELHERKECTGGPTVAAE 337
ELH+ C+ PT+ A+
Sbjct: 446 PELHKNNMCSTSPTLPAD 393
>gb|BI397792.1|BI397792 NXPV_107_E10_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_107_E10 5' similar to Arabidopsis
thaliana sequence At3g14960 galactosyltransferase,
putative see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 267
Score = 107 bits (228), Expect = 9e-023
Identities = 43/76 (56%), Positives = 50/76 (65%)
Frame = +2 / +2
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EP LLGS+YF+HAYGPIY RN+S+RMFSNEDVTIG+WMLAMNVNH
Sbjct: 38 YEPLGDLLGSEYFIHAYGPIYALSAEIVAGLSGLRNDSYRMFSNEDVTIGAWMLAMNVNH 217
Query: 182 ENTHALCEPDCTESSI 229
E+ LC SI
Sbjct: 218 EDNRELCSXXXXXXSI 265
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 383,422,558
Number of Sequences: 355925
Number of extensions: 5729121
Number of successful extensions: 41405
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 9112082005
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)