TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3866402.2.1
(406 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AI812992.1|AI812992 2E10 Pine Lambda Zap Xylem library P... 153 7e-037
gb|BF221028.1|BF221028 NXCI_162_C02_F NXCI (Nsf Xylem Compr... 146 8e-035
gb|CF397463.1|CF397463 RTDS3_3_G03.g1_A022 Drought-stressed... 145 2e-034
gb|DR020869.1|DR020869 STRS1_39_G06.g1_A034 Shoot tip pitch... 145 2e-034
gb|DR060520.1|DR060520 RTNIT1_28_H03.b1_A029 Roots minus ni... 145 2e-034
gb|CO158939.1|CO158939 FLD1_10_F07.b1_A029 Root flooded Pin... 143 6e-034
gb|CO167598.1|CO167598 FLD1_70_A08.b1_A029 Root flooded Pin... 143 6e-034
gb|CO167675.1|CO167675 FLD1_70_A08.g1_A029 Root flooded Pin... 143 6e-034
gb|DR071603.1|DR071603 RTDK1_21_A05.b1_A029 Roots, dark Pin... 143 6e-034
gb|DR386010.1|DR386010 RTHG1_12_G05.b1_A029 Roots plus adde... 143 6e-034
gb|DR386081.1|DR386081 RTHG1_12_G05.g1_A029 Roots plus adde... 143 6e-034
gb|BX678794.1|BX678794 BX678794 RS Pinus pinaster cDNA clon... 141 2e-033
gb|BX678600.1|BX678600 BX678600 RS Pinus pinaster cDNA clon... 135 1e-031
gb|BF516675.1|BF516675 NXSI_002_B01_F NXSI (Nsf Xylem Side ... 129 1e-029
gb|DR048150.1|DR048150 RTBOR1_6_H10.g2_A029 Roots plus adde... 122 1e-027
gb|CF394902.1|CF394902 RTDS2_8_B01.b1_A021 Drought-stressed... 122 2e-027
gb|DR060360.1|DR060360 RTNIT1_27_G11.b1_A029 Roots minus ni... 121 2e-027
gb|BF049746.1|BF049746 NXCI_106_F09_F NXCI (Nsf Xylem Compr... 116 6e-026
gb|DR117496.1|DR117496 RTMG1_7_G10.b1_A029 Roots minus magn... 103 8e-022
gb|DR117586.1|DR117586 RTMG1_7_G10.g1_A029 Roots minus magn... 103 8e-022
gb|CD021034.1|CD021034 NXNV_126_G03_F Nsf Xylem Normal wood... 102 1e-021
>gb|AI812992.1|AI812992 2E10 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA sequence
Length = 675
Score = 153 bits (328), Expect = 7e-037
Identities = 60/78 (76%), Positives = 69/78 (88%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWAILQI+Q KP + G WGSV+AL+RGYE++MG+++F PVAVLAWFPFVSEFQ RL
Sbjct: 439 TGWAILQIAQTCKPFVHRIGFWGSVRALARGYEFIMGLLLFAPVAVLAWFPFVSEFQTRL 618
Query: 224 LFNQAFSRGLQISRILAG 171
LFNQAFSRGLQISRILAG
Sbjct: 619 LFNQAFSRGLQISRILAG 672
>gb|BF221028.1|BF221028 NXCI_162_C02_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_162_C02 5' similar to Arabidopsis
thaliana sequence At2g31960 glucan synthase like protein
see http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 381
Score = 146 bits (313), Expect = 8e-035
Identities = 57/81 (70%), Positives = 67/81 (82%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWAILQI+Q K + G WGSV+AL+RGYE++MG+++F P+AVLAWFPFVSEFQ RL
Sbjct: 16 TGWAILQIAQTCKSFVHRIGFWGSVRALARGYEFIMGLLLFAPIAVLAWFPFVSEFQTRL 195
Query: 224 LFNQAFSRGLQISRILAGGKK 162
LFNQ F GLQISRILAG KK
Sbjct: 196 LFNQXFXXGLQISRILAGRKK 258
>gb|CF397463.1|CF397463 RTDS3_3_G03.g1_A022 Drought-stressed loblolly pine roots DS3 Pinus
taeda cDNA clone RTDS3_3_G03_A022 5', mRNA sequence
Length = 773
Score = 145 bits (311), Expect = 2e-034
Identities = 58/81 (71%), Positives = 70/81 (86%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA+LQI+QA +P+ G SV++L+RGYEY+MG+++F PVA+LAWFPFVSEFQ RL
Sbjct: 377 TGWALLQIAQACRPLYNRTGFLESVRSLARGYEYIMGLLLFTPVAILAWFPFVSEFQTRL 556
Query: 224 LFNQAFSRGLQISRILAGGKK 162
LFNQAFSRGLQISRILAG KK
Sbjct: 557 LFNQAFSRGLQISRILAGRKK 619
>gb|DR020869.1|DR020869 STRS1_39_G06.g1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_39_G06_A034 5', mRNA sequence
Length = 683
Score = 145 bits (311), Expect = 2e-034
Identities = 58/81 (71%), Positives = 70/81 (86%)
Frame = -3 / -2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA+LQI+QA +P+ G SV++L+RGYEY+MG+++F PVA+LAWFPFVSEFQ RL
Sbjct: 532 TGWALLQIAQACRPLYNRTGFLESVRSLARGYEYIMGLLLFTPVAILAWFPFVSEFQTRL 353
Query: 224 LFNQAFSRGLQISRILAGGKK 162
LFNQAFSRGLQISRILAG KK
Sbjct: 352 LFNQAFSRGLQISRILAGRKK 290
>gb|DR060520.1|DR060520 RTNIT1_28_H03.b1_A029 Roots minus nitrogen Pinus taeda cDNA clone
RTNIT1_28_H03_A029 3', mRNA sequence
Length = 692
Score = 145 bits (311), Expect = 2e-034
Identities = 58/81 (71%), Positives = 70/81 (86%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA+LQI+QA +P+ G SV++L+RGYEY+MG+++F PVA+LAWFPFVSEFQ RL
Sbjct: 50 TGWALLQIAQACRPLYNRTGFLESVRSLARGYEYIMGLLLFTPVAILAWFPFVSEFQTRL 229
Query: 224 LFNQAFSRGLQISRILAGGKK 162
LFNQAFSRGLQISRILAG KK
Sbjct: 230 LFNQAFSRGLQISRILAGRKK 292
>gb|CO158939.1|CO158939 FLD1_10_F07.b1_A029 Root flooded Pinus taeda cDNA clone
FLD1_10_F07_A029 3', mRNA sequence
Length = 760
Score = 143 bits (307), Expect = 6e-034
Identities = 56/80 (70%), Positives = 68/80 (85%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+++ G W SV+AL+R Y+Y+MG+V+F PVA+LAWFPFVSEFQ R
Sbjct: 28 TGWAFLQIALACRPLLQRAGFWDSVRALARSYDYVMGLVLFTPVAILAWFPFVSEFQTRF 207
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGLQISRILAG K
Sbjct: 208 LFNQAFSRGLQISRILAGRK 267
>gb|CO167598.1|CO167598 FLD1_70_A08.b1_A029 Root flooded Pinus taeda cDNA clone
FLD1_70_A08_A029 3', mRNA sequence
Length = 820
Score = 143 bits (307), Expect = 6e-034
Identities = 57/80 (71%), Positives = 68/80 (85%)
Frame = -3 / +3
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+ + GLW SV+AL+R YEY+MG+ +F PVA+LAWFPFVSEFQ+RL
Sbjct: 90 TGWAFLQIALACRPLFECCGLWDSVRALARSYEYVMGLHLFTPVAILAWFPFVSEFQSRL 269
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGLQISRIL G K
Sbjct: 270 LFNQAFSRGLQISRILTGRK 329
>gb|CO167675.1|CO167675 FLD1_70_A08.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_70_A08_A029 5', mRNA sequence
Length = 501
Score = 143 bits (307), Expect = 6e-034
Identities = 57/80 (71%), Positives = 68/80 (85%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+ + GLW SV+AL+R YEY+MG+ +F PVA+LAWFPFVSEFQ+RL
Sbjct: 98 TGWAFLQIALACRPLFECCGLWDSVRALARSYEYVMGLHLFTPVAILAWFPFVSEFQSRL 277
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGLQISRIL G K
Sbjct: 278 LFNQAFSRGLQISRILTGRK 337
>gb|DR071603.1|DR071603 RTDK1_21_A05.b1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_21_A05_A029 3', mRNA sequence
Length = 769
Score = 143 bits (307), Expect = 6e-034
Identities = 56/80 (70%), Positives = 68/80 (85%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+++ G W SV+AL+R Y+Y+MG+V+F PVA+LAWFPFVSEFQ R
Sbjct: 160 TGWAFLQIALACRPLLQRAGFWDSVRALARSYDYVMGLVLFTPVAILAWFPFVSEFQTRF 339
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGLQISRILAG K
Sbjct: 340 LFNQAFSRGLQISRILAGRK 399
>gb|DR386010.1|DR386010 RTHG1_12_G05.b1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_12_G05_A029 3', mRNA sequence
Length = 806
Score = 143 bits (307), Expect = 6e-034
Identities = 56/80 (70%), Positives = 68/80 (85%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+++ G W SV+AL+R Y+Y+MG+V+F PVA+LAWFPFVSEFQ R
Sbjct: 13 TGWAFLQIALACRPLLQRAGFWDSVRALARSYDYVMGLVLFTPVAILAWFPFVSEFQTRF 192
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGLQISRILAG K
Sbjct: 193 LFNQAFSRGLQISRILAGRK 252
>gb|DR386081.1|DR386081 RTHG1_12_G05.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_12_G05_A029 5', mRNA sequence
Length = 804
Score = 143 bits (307), Expect = 6e-034
Identities = 56/80 (70%), Positives = 68/80 (85%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+++ G W SV+AL+R Y+Y+MG+V+F PVA+LAWFPFVSEFQ R
Sbjct: 157 TGWAFLQIALACRPLLQRAGFWDSVRALARSYDYVMGLVLFTPVAILAWFPFVSEFQTRF 336
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGLQISRILAG K
Sbjct: 337 LFNQAFSRGLQISRILAGRK 396
>gb|BX678794.1|BX678794 BX678794 RS Pinus pinaster cDNA clone RS15C01, mRNA sequence
Length = 488
Score = 141 bits (303), Expect = 2e-033
Identities = 55/80 (68%), Positives = 68/80 (85%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+++ G W SV+AL+R Y+Y+MG+++F PVA+LAWFPFVSEFQ R
Sbjct: 131 TGWAFLQIALACRPLLQHAGFWDSVRALARSYDYVMGLLLFTPVAILAWFPFVSEFQTRF 310
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGLQISRILAG K
Sbjct: 311 LFNQAFSRGLQISRILAGRK 370
>gb|BX678600.1|BX678600 BX678600 RS Pinus pinaster cDNA clone RS11G06, mRNA sequence
Length = 551
Score = 135 bits (290), Expect = 1e-031
Identities = 53/80 (66%), Positives = 66/80 (82%)
Frame = -3 / +3
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWA LQI+ A +P+++ G W SV+AL+R Y+Y+MG+++F PVA+LAWFPFVSEFQ R
Sbjct: 123 TGWAFLQIALACRPLLQHAGFWDSVRALARSYDYVMGLLLFTPVAILAWFPFVSEFQTRF 302
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGL SRILAG K
Sbjct: 303 LFNQAFSRGLXNSRILAGRK 362
>gb|BF516675.1|BF516675 NXSI_002_B01_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_002_B01 5' similar to Arabidopsis thaliana
sequence At5g13000 callose synthase catalytic subunit
-like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 447
Score = 129 bits (276), Expect = 1e-029
Identities = 51/62 (82%), Positives = 58/62 (93%)
Frame = -3 / +1
Query: 347 GLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQAFSRGLQISRILAGG 168
G WGSV+AL+RGYE++MG+++F PVAVLAWFPFVSEFQ RLLFNQAFSRGLQISRILAG
Sbjct: 7 GFWGSVRALARGYEFIMGLLLFAPVAVLAWFPFVSEFQTRLLFNQAFSRGLQISRILAGR 186
Query: 167 KK 162
KK
Sbjct: 187 KK 192
>gb|DR048150.1|DR048150 RTBOR1_6_H10.g2_A029 Roots plus added boron Pinus taeda cDNA clone
RTBOR1_6_H10_A029 5', mRNA sequence
Length = 691
Score = 122 bits (261), Expect = 1e-027
Identities = 49/80 (61%), Positives = 63/80 (78%)
Frame = -3 / -2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW I+ ++ K V+K+ GLW SV A++R Y+ MGI++F+P+ VL+WFPFVS FQ RL
Sbjct: 579 TGWLIICLAITWKSVVKSLGLWKSVSAIARFYDAGMGILLFIPIVVLSWFPFVSTFQTRL 400
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFNQAFSRGL+IS ILAG K
Sbjct: 399 LFNQAFSRGLEISLILAGNK 340
>gb|CF394902.1|CF394902 RTDS2_8_B01.b1_A021 Drought-stressed loblolly pine roots DS2 Pinus
taeda cDNA clone RTDS2_8_B01_A021 3', mRNA sequence
Length = 559
Score = 122 bits (260), Expect = 2e-027
Identities = 47/80 (58%), Positives = 63/80 (78%)
Frame = -3 / +3
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW IL ++ A KP++K GLW S+++L R Y+ MG+++F+PVA+ +W PFVS FQ RL
Sbjct: 39 TGWGILCLAIAWKPIVKRLGLWKSIRSLGRLYDAGMGMIVFIPVALCSWLPFVSTFQTRL 218
Query: 224 LFNQAFSRGLQISRILAGGK 165
+FNQAFSRGL+IS ILAG K
Sbjct: 219 MFNQAFSRGLEISLILAGNK 278
>gb|DR060360.1|DR060360 RTNIT1_27_G11.b1_A029 Roots minus nitrogen Pinus taeda cDNA clone
RTNIT1_27_G11_A029 3', mRNA sequence
Length = 569
Score = 121 bits (259), Expect = 2e-027
Identities = 47/80 (58%), Positives = 62/80 (77%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW IL + A KP++K GLW S+++L R Y+ MG+++F+PVA+ +W PFVS FQ RL
Sbjct: 14 TGWGILCLGIAWKPIVKRLGLWKSIRSLGRLYDAGMGMIVFIPVAMCSWLPFVSTFQTRL 193
Query: 224 LFNQAFSRGLQISRILAGGK 165
+FNQAFSRGL+IS ILAG K
Sbjct: 194 MFNQAFSRGLEISLILAGNK 253
>gb|BF049746.1|BF049746 NXCI_106_F09_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_106_F09 5' similar to Arabidopsis
thaliana sequence At2g31960 glucan synthase like protein
see http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 213
Score = 116 bits (249), Expect = 6e-026
Identities = 44/66 (66%), Positives = 53/66 (80%)
Frame = -3 / +1
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGWAILQI+Q K + G WGSV+AL+RGYE++MG+++F P AVLAWFPFVSEFQ RL
Sbjct: 16 TGWAILQIAQTCKXFVHRIGFWGSVRALARGYEFIMGLLLFAPXAVLAWFPFVSEFQTRL 195
Query: 224 LFNQAF 207
LFN F
Sbjct: 196 LFNXXF 213
>gb|DR117496.1|DR117496 RTMG1_7_G10.b1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_7_G10_A029 3', mRNA sequence
Length = 872
Score = 103 bits (219), Expect = 8e-022
Identities = 40/80 (50%), Positives = 57/80 (71%)
Frame = -3 / +2
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW ++ I+Q +P+++ +W V A++R YE + GI+I +PVA+L+W P Q R+
Sbjct: 371 TGWGLISIAQVLRPLLQPTMVWEIVVAVARLYEMIFGIIIMIPVALLSWLPGFQTLQTRI 550
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFN+AFSRGLQISRIL G K
Sbjct: 551 LFNEAFSRGLQISRILVGKK 610
>gb|DR117586.1|DR117586 RTMG1_7_G10.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_7_G10_A029 5', mRNA sequence
Length = 763
Score = 103 bits (219), Expect = 8e-022
Identities = 40/80 (50%), Positives = 57/80 (71%)
Frame = -3 / +3
Query: 404 TGWAILQISQASKPVIKAFGLWGSVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARL 225
TGW ++ I+Q +P+++ +W V A++R YE + GI+I +PVA+L+W P Q R+
Sbjct: 504 TGWGLISIAQVLRPLLQPTMVWEIVVAVARLYEMIFGIIIMIPVALLSWLPGFQTLQTRI 683
Query: 224 LFNQAFSRGLQISRILAGGK 165
LFN+AFSRGLQISRIL G K
Sbjct: 684 LFNEAFSRGLQISRILVGKK 743
>gb|CD021034.1|CD021034 NXNV_126_G03_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_126_G03 5' similar to Arabidopsis thaliana
sequence At1g05570 putative glucan synthase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 171
Score = 102 bits (218), Expect = 1e-021
Identities = 41/55 (74%), Positives = 48/55 (87%)
Frame = -3 / +2
Query: 335 SVKALSRGYEYLMGIVIFVPVAVLAWFPFVSEFQARLLFNQAFSRGLQISRILAG 171
SV+AL+RGYE++MG+++F PVAVLAWFPFVSEFQ RLLFN FS GL SRILAG
Sbjct: 2 SVRALARGYEFIMGLLLFAPVAVLAWFPFVSEFQTRLLFNXXFSXGLXXSRILAG 166
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 243,750,814
Number of Sequences: 355925
Number of extensions: 3507422
Number of successful extensions: 84612
Number of sequences better than 1.0e-020: 27
length of database: 72,425,745
effective HSP length: 50
effective length of database: 54,629,495
effective search space used: 4588877580
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)