TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3848982.2.1
(697 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus adde... 113 6e-034
gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus adde... 113 1e-032
gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compr... 110 2e-028
gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip libra... 106 2e-027
gb|DR387045.1|DR387045 RTHG1_19_C01.b1_A029 Roots plus adde... 86 1e-025
gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinu... 113 1e-024
gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus adde... 113 1e-024
gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added... 111 4e-024
gb|DR385374.1|DR385374 RTHG1_8_F09.b1_A029 Roots plus added... 80 5e-024
>gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 5', mRNA sequence
Length = 707
Score = 113 bits (242), Expect(2) = 6e-034
Identities = 47/98 (47%), Positives = 62/98 (63%)
Frame = +3 / -2
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSGTAF MA G + L N G + V + RV C + G +IAF V+ G++ + A+ I
Sbjct: 697 HFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILI 518
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSG 344
EYE GDGDLRA +KQ+ + W M+ +WGA W SG
Sbjct: 517 EYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNSG 404
Score = 50.6 bits (104), Expect(2) = 6e-034
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = +3 / -2
Query: 390 SGRVLVASNVIPAGWQPGRTYRSVVNF 470
+GR L A+NVIP WQPG TYRS+VN+
Sbjct: 361 TGRTLTANNVIPQNWQPGATYRSLVNY 281
>gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 5', mRNA sequence
Length = 821
Score = 113 bits (242), Expect(2) = 1e-032
Identities = 47/98 (47%), Positives = 62/98 (63%)
Frame = +3 / +3
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSGTAF MA G + L N G + V + RV C + G +IAF V+ G++ + A+ I
Sbjct: 411 HFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILI 590
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSG 344
EYE GDGDLRA +KQ+ + W M+ +WGA W SG
Sbjct: 591 EYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNSG 704
Score = 46.4 bits (95), Expect(2) = 1e-032
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +3 / +3
Query: 390 SGRVLVASNVIPAGWQPGRTYRSVV 464
+GR L A+NVIP WQPG TYRS+V
Sbjct: 747 TGRTLTANNVIPQNWQPGATYRSLV 821
>gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_022_D05 5' similar to Arabidopsis
thaliana sequence At4g28250 putative
beta-expansin/allergen protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 458
Score = 110 bits (234), Expect(2) = 2e-028
Identities = 47/99 (47%), Positives = 57/99 (57%)
Frame = +3 / +1
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSG AFG MA G+ L N G L V Y R C + G +I F V GS A + ++ I
Sbjct: 13 HFDLSGAAFGRMATNGKTPALLNTGELPVLYRRTLCEYPGKNITFHVTEGSTAYWFSILI 192
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSGS 347
EYE GDGD+ A LKQ+ + AW M WGA W G+
Sbjct: 193 EYEDGDGDVGAMHLKQADSDAWMEMNHVWGANWCLLGGT 309
Score = 35.4 bits (71), Expect(2) = 2e-028
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = +3 / +1
Query: 381 TSGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
T S R L A NVIP W P TY++ +NF
Sbjct: 343 TLTSKRTLSARNVIPDHWYPNATYKARLNF 432
>gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST17B02, mRNA sequence
Length = 598
Score = 106 bits (226), Expect(2) = 2e-027
Identities = 45/93 (48%), Positives = 56/93 (60%)
Frame = +3 / +2
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSG AFG MA G+ L N+G L V Y R C + G +I F V GS A + ++ I
Sbjct: 143 HFDLSGAAFGHMATNGKTPALLNSGELPVLYRRTLCEYPGKNITFHVTEGSTAYWFSILI 322
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVW 329
YE GDGD+ A LKQ+ + AW M+ WGA W
Sbjct: 323 GYEDGDGDVGAMHLKQAESDAWMEMRHVWGANW 421
Score = 35.9 bits (72), Expect(2) = 2e-027
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = +3 / +2
Query: 381 TSGSGRVLVASNVIPAGWQPGRTYRSVVNF 470
T S R L A NVIP W P TY++ +NF
Sbjct: 473 TLTSKRTLSARNVIPEHWYPNATYKARLNF 562
>gb|DR387045.1|DR387045 RTHG1_19_C01.b1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 3', mRNA sequence
Length = 725
Score = 85.8 bits (181), Expect(2) = 1e-025
Identities = 34/76 (44%), Positives = 48/76 (63%)
Frame = +3 / +2
Query: 117 NAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAIEYESGDGDLRAXELKQSGAAAW 296
N G + V + RV C + G +IAF V+ G++ + A+ IEYE GDGDLRA +KQ+ + W
Sbjct: 5 NTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILIEYEDGDGDLRAVYIKQANSNTW 184
Query: 297 APMQRSWGAVWQYKSG 344
M+ +WGA W SG
Sbjct: 185 QGMEETWGANWCLNSG 232
Score = 50.6 bits (104), Expect(2) = 1e-025
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = +3 / +2
Query: 390 SGRVLVASNVIPAGWQPGRTYRSVVNF 470
+GR L A+NVIP WQPG TYRS+VN+
Sbjct: 275 TGRTLTANNVIPQNWQPGATYRSLVNY 355
>gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_9_H09_A029 5', mRNA sequence
Length = 806
Score = 113 bits (242), Expect = 1e-024
Identities = 47/98 (47%), Positives = 62/98 (63%)
Frame = +3 / +2
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSGTAF MA G + L N G + V + RV C + G +IAF V+ G++ + A+ I
Sbjct: 440 HFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILI 619
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSG 344
EYE GDGDLRA +KQ+ + W M+ +WGA W SG
Sbjct: 620 EYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNSG 733
>gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 3', mRNA sequence
Length = 793
Score = 113 bits (242), Expect = 1e-024
Identities = 47/98 (47%), Positives = 62/98 (63%)
Frame = +3 / -1
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSGTAF MA G + L N G + V + RV C + G +IAF V+ G++ + A+ I
Sbjct: 340 HFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILI 161
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSG 344
EYE GDGDLRA +KQ+ + W M+ +WGA W SG
Sbjct: 160 EYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNSG 47
>gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_8_F09_A029 5', mRNA sequence
Length = 717
Score = 111 bits (238), Expect = 4e-024
Identities = 46/98 (46%), Positives = 61/98 (62%)
Frame = +3 / +3
Query: 51 HFDLSGTAFGAMAKPGQADQLRNAGRLQVQYIRVACNWNGLDIAFRVDAGSNANYLAMAI 230
HFDLSGTAF MA G + L N G + V + RV C + G +IAF V+ G++ + A+ I
Sbjct: 411 HFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILI 590
Query: 231 EYESGDGDLRAXELKQSGAAAWAPMQRSWGAVWQYKSG 344
EYE GDGDLRA +KQ+ + W M+ +WG W SG
Sbjct: 591 EYEDGDGDLRAVYIKQANSNTWQGMEETWGCNWCLNSG 704
>gb|DR385374.1|DR385374 RTHG1_8_F09.b1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_8_F09_A029 3', mRNA sequence
Length = 695
Score = 80.3 bits (169), Expect(2) = 5e-024
Identities = 31/66 (46%), Positives = 43/66 (65%)
Frame = +3 / +2
Query: 147 RVACNWNGLDIAFRVDAGSNANYLAMAIEYESGDGDLRAXELKQSGAAAWAPMQRSWGAV 326
RV C + G +IAF V+ G++ + A+ IEYE GDGDLRA +KQ+ + W M+ +WGA
Sbjct: 8 RVPCEYPGKNIAFNVNGGASDYWFAILIEYEDGDGDLRAVYIKQANSNTWQGMEETWGAN 187
Query: 327 WQYKSG 344
W SG
Sbjct: 188 WCLNSG 205
Score = 50.6 bits (104), Expect(2) = 5e-024
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = +3 / +2
Query: 390 SGRVLVASNVIPAGWQPGRTYRSVVNF 470
+GR L A+NVIP WQPG TYRS+VN+
Sbjct: 248 TGRTLTANNVIPQNWQPGATYRSLVNY 328
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 338,458,935
Number of Sequences: 355925
Number of extensions: 4389952
Number of successful extensions: 50544
Number of sequences better than 1.0e-020: 12
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 9651258455
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)