TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3802485.2.1
(1118 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR117345.1|DR117345 RTMG1_6_A01.g1_A029 Roots minus magn... 182 2e-064
gb|AI812897.1|AI812897 22C2 Pine Lambda Zap Xylem library P... 139 5e-047
gb|CX646459.1|CX646459 COLD1_9_B03.g1_A029 Root cold Pinus ... 177 5e-045
gb|CO363960.1|CO363960 RTK1_12_G03.g1_A029 Roots minus pota... 155 7e-037
gb|BQ701707.1|BQ701707 NXSI_118_F08_F NXSI (Nsf Xylem Side ... 121 9e-027
gb|CO363866.1|CO363866 RTK1_12_G03.b1_A029 Roots minus pota... 112 4e-024
gb|BF609284.1|BF609284 NXSI_042_E06_F NXSI (Nsf Xylem Side ... 104 1e-021
>gb|DR117345.1|DR117345 RTMG1_6_A01.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_6_A01_A029 5', mRNA sequence
Length = 842
Score = 182 bits (392), Expect(3) = 2e-064
Identities = 75/137 (54%), Positives = 99/137 (72%)
Frame = +2 / +3
Query: 596 ILSVTKARSILLNGAVRKGERLVPPVSFDLFMRATFPVSNARVKATERFEAAYPMIKELA 775
IL TKAR ILLNGA+RKGER+VPP + +L M+A+FP+ +AR K TERF A YP +KELA
Sbjct: 252 ILENTKARPILLNGAIRKGERIVPPSALNLLMQASFPLDSARTKGTERFVAIYPFLKELA 431
Query: 776 LAGPPGSKTVKQASQQLLPLCAKAMQENNAELTREAVDVFIWCLTQNAESYKXXERIYLE 955
L G SK + +QQLLPL A A+ E N LT EA VFIWCLTQN++ YK E++++E
Sbjct: 432 LTGSHRSKATRPVAQQLLPLMATAISEENPGLTNEASGVFIWCLTQNSDCYKQWEKLHIE 611
Query: 956 NIEAXXAVLSKVVIDWR 1006
+I+A +L K+ +W+
Sbjct: 612 HIKASTIILEKLSAEWK 662
Score = 78.5 bits (165), Expect(3) = 2e-064
Identities = 34/68 (50%), Positives = 43/68 (63%)
Frame = +2 / +3
Query: 347 VVLAMTLRRKPDVLVNVMPKIMGNNKYLGQEKLPIIVWVIAQASQGDLVSGMFCWAHSLF 526
VVLAM LRR+PD+L+ + P I G + Q++LP+IVW IAQ Q DLV GM W L
Sbjct: 3 VVLAMVLRRRPDILLQLSPTIKGEAQIQVQDRLPMIVWSIAQVCQVDLVIGMSTWVQILL 182
Query: 527 PTLCAKSS 550
P K+S
Sbjct: 183 PLAVGKTS 206
Score = 68.9 bits (144), Expect(3) = 3e-026
Identities = 33/59 (55%), Positives = 38/59 (64%)
Frame = -3 / -2
Query: 783 PARANSLIIG*AASNLSVAFTRALETGNVALMNKSNETGGTKRSPFLTAPFNNIERALV 607
P RANS G A+NLSV F RA +G A + + + GGT RSPFL APFN I RALV
Sbjct: 439 PVRANSFKNGYIATNLSVPFVRAESSGKEACIKRLSADGGTIRSPFLIAPFNRIGRALV 263
Score = 51.0 bits (105), Expect(3) = 3e-026
Identities = 23/41 (56%), Positives = 26/41 (63%)
Frame = -3 / -2
Query: 939 SXCLYDSAF*VKHQMKTSTASLVSSALFSCIAFAHKGKSCC 817
S CL S F*V+HQ+KT AS V S IA A KG+SCC
Sbjct: 595 SHCL*QSEF*VRHQIKTPEASFVKPGFSSLIAVAIKGRSCC 473
Score = 37.3 bits (75), Expect(3) = 3e-026
Identities = 21/61 (34%), Positives = 32/61 (52%)
Frame = -3 / -2
Query: 528 GNRE*AQQNMPDTRSP*EA*AITQTMMGSFSCPRYLLFPIIFGITFTSTSGFLLKVMART 349
G R ++P TRS + AI T++G+ S P+I G + + SG LL +A+T
Sbjct: 184 GRRICTHVDIPITRSTWQTWAIDHTIIGNLS*T*ICASPLIVGESCSKMSGLLLNTIAKT 5
Query: 348 T 346
T
Sbjct: 4 T 2
Score = 24.9 bits (48), Expect(3) = 2e-064
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +2 / +3
Query: 533 LCAKSSGNPLARDLVLQLLE 592
L + PL+RDL LQL+E
Sbjct: 186 LAVGKTSTPLSRDLSLQLIE 245
>gb|AI812897.1|AI812897 22C2 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA sequence
Length = 556
Score = 139 bits (299), Expect(4) = 5e-047
Identities = 61/102 (59%), Positives = 72/102 (70%)
Frame = +2 / +2
Query: 596 ILSVTKARSILLNGAVRKGERLVPPVSFDLFMRATFPVSNARVKATERFEAAYPMIKELA 775
IL TKAR ILLNGA+RKGER+VPP + +L M+A FP+ +AR K TERF A YP +KELA
Sbjct: 146 ILENTKARPILLNGAIRKGERIVPPSALNLLMQAAFPLDSARTKGTERFVAIYPFLKELA 325
Query: 776 LAGPPGSKTVKQASQQLLPLCAKAMQENNAELTREAVDVFIW 901
L G SK + +QQLLPL A A+ E N LT EA VFIW
Sbjct: 326 LTGSHRSKATRPVAQQLLPLMATAISEENPGLTNEASGVFIW 451
Score = 40.9 bits (83), Expect(4) = 5e-047
Identities = 13/34 (38%), Positives = 25/34 (73%)
Frame = +2 / +1
Query: 905 LTQNAESYKXXERIYLENIEAXXAVLSKVVIDWR 1006
LTQN++ YK E++++E+I+A +L K+ +W+
Sbjct: 454 LTQNSDCYKQWEKLHIEHIKASTIILEKLSAEWK 555
Score = 38.6 bits (78), Expect(4) = 5e-047
Identities = 17/33 (51%), Positives = 18/33 (54%)
Frame = +2 / +2
Query: 452 IVWVIAQASQGDLVSGMFCWAHSLFPTLCAKSS 550
IVW IAQ Q DLV GM W L P K+S
Sbjct: 2 IVWSIAQVCQVDLVIGMSTWVQILLPLAVGKTS 100
Score = 25.8 bits (50), Expect(4) = 5e-047
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +2 / +2
Query: 533 LCAKSSGNPLARDLVLQLLE 592
L + PL+RDL LQL+E
Sbjct: 80 LAVGKTSTPLSRDLTLQLIE 139
>gb|CX646459.1|CX646459 COLD1_9_B03.g1_A029 Root cold Pinus taeda cDNA clone COLD1_9_B03_A029
5', mRNA sequence
Length = 862
Score = 177 bits (382), Expect(2) = 5e-045
Identities = 73/137 (53%), Positives = 96/137 (70%)
Frame = +2 / +3
Query: 596 ILSVTKARSILLNGAVRKGERLVPPVSFDLFMRATFPVSNARVKATERFEAAYPMIKELA 775
IL T R ILLNGA+RKGER+VPP + +L M+A FP+ +AR K TERF A YP +KELA
Sbjct: 63 ILENTNVRPILLNGAIRKGERIVPPSALNLLMQAAFPLDSARTKGTERFVAIYPFLKELA 242
Query: 776 LAGPPGSKTVKQASQQLLPLCAKAMQENNAELTREAVDVFIWCLTQNAESYKXXERIYLE 955
L G SK + +QQLLPL A A+ E N LT EA VFIWCLTQN++ YK E++++E
Sbjct: 243 LTGSHRSKATRPVAQQLLPLMATAISEENPGLTNEASGVFIWCLTQNSDCYKQWEKLHIE 422
Query: 956 NIEAXXAVLSKVVIDWR 1006
+I+A +L K+ +W+
Sbjct: 423 HIKASTIILEKLSAEWK 473
Score = 24.4 bits (47), Expect(2) = 5e-045
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +2 / +3
Query: 548 SGNPLARDLVLQLLE 592
+ PL+RDL LQL+E
Sbjct: 12 NSTPLSRDLFLQLIE 56
>gb|CO363960.1|CO363960 RTK1_12_G03.g1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_12_G03_A029 5', mRNA sequence
Length = 745
Score = 155 bits (332), Expect = 7e-037
Identities = 62/120 (51%), Positives = 84/120 (70%)
Frame = +2 / +2
Query: 647 KGERLVPPVSFDLFMRATFPVSNARVKATERFEAAYPMIKELALAGPPGSKTVKQASQQL 826
KGER+VPP + +L M+A FP+ +AR K TERF A YP +KELAL G SK + +QQL
Sbjct: 2 KGERIVPPSALNLLMQAAFPLDSARTKGTERFVAIYPFLKELALTGSHRSKATRPVAQQL 181
Query: 827 LPLCAKAMQENNAELTREAVDVFIWCLTQNAESYKXXERIYLENIEAXXAVLSKVVIDWR 1006
LPL A A+ E N LT EA VFIWCLTQN++ YK E++++E+I+A +L K+ +W+
Sbjct: 182 LPLMATAISEENPGLTNEASGVFIWCLTQNSDCYKQWEKLHIEHIKASTIILEKLSAEWK 361
>gb|BQ701707.1|BQ701707 NXSI_118_F08_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_118_F08 5' similar to Arabidopsis thaliana
sequence At1g23170 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA sequence
Length = 384
Score = 121 bits (259), Expect = 9e-027
Identities = 49/98 (50%), Positives = 66/98 (67%)
Frame = +2 / +2
Query: 713 NARVKATERFEAAYPMIKELALAGPPGSKTVKQASQQLLPLCAKAMQENNAELTREAVDV 892
+AR K TERF A YP +KELAL G SK + +QQLLPL A A+ E N LT EA V
Sbjct: 2 SARTKGTERFVAIYPFLKELALTGSHRSKATRPVAQQLLPLMATAISEENPSLTNEASGV 181
Query: 893 FIWCLTQNAESYKXXERIYLENIEAXXAVLSKVVIDWR 1006
FIWCLT N++ YK E++++E+I+A +L K+ +W+
Sbjct: 182 FIWCLTXNSDCYKQWEKLHIEHIKASTIILXKLSAEWK 295
>gb|CO363866.1|CO363866 RTK1_12_G03.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_12_G03_A029 3', mRNA sequence
Length = 860
Score = 112 bits (240), Expect = 4e-024
Identities = 45/91 (49%), Positives = 62/91 (68%)
Frame = +2 / +3
Query: 734 ERFEAAYPMIKELALAGPPGSKTVKQASQQLLPLCAKAMQENNAELTREAVDVFIWCLTQ 913
E+F A YP +KELAL G SK + +QQLLPL A A+ E N LT EA VFIWCLTQ
Sbjct: 3 EKFVAIYPFLKELALTGSHRSKATRPVAQQLLPLMATAISEENPGLTNEASGVFIWCLTQ 182
Query: 914 NAESYKXXERIYLENIEAXXAVLSKVVIDWR 1006
N++ YK E++++E+I+A +L K+ +W+
Sbjct: 183 NSDCYKQWEKLHIEHIKASTIILEKLSAEWK 275
>gb|BF609284.1|BF609284 NXSI_042_E06_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_042_E06 5' similar to Arabidopsis thaliana
sequence At1g70770 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 530
Score = 104 bits (222), Expect = 1e-021
Identities = 41/80 (51%), Positives = 59/80 (73%)
Frame = +2 / +3
Query: 5 ISSSYENQQDIQLMRFADYFGRAFVAVSASQFAWAKMFKESTVSKMVDIPLCHIPEAVIK 184
I++SYE D+QLMR RAF +V+++QF K+ KESTV+K+ +IPLC+IPEAV K
Sbjct: 282 ITASYEKXADVQLMRXXXXXARAFSSVNSAQFPLNKILKESTVAKLAEIPLCYIPEAVSK 461
Query: 185 TASDWISQRSSDALGDFVLW 244
T++DWI+Q+ ++ L F LW
Sbjct: 462 TSADWINQQPTEELNKFXLW 521
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 577,901,036
Number of Sequences: 355925
Number of extensions: 9011047
Number of successful extensions: 40705
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 17086188680
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)