TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3748516.2.1
(1155 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus adde... 118 7e-045
gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinu... 118 6e-040
gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus adde... 118 6e-040
gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added... 117 1e-039
gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus adde... 118 2e-031
gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus adde... 84 2e-030
gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip libra... 112 2e-029
gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compr... 112 4e-024
gb|DR387045.1|DR387045 RTHG1_19_C01.b1_A029 Roots plus adde... 88 3e-022
>gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 5', mRNA sequence
Length = 821
Score = 118 bits (253), Expect(4) = 7e-045
Identities = 46/95 (48%), Positives = 63/95 (66%)
Frame = -3 / +3
Query: 664 KYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAV 485
K HFDLSGTAF MAS G + L + G++ + FRRVPC +PG I F V G++ + A+
Sbjct: 405 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 584
Query: 484 LIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIW 380
LIE+E+ DGD+ I Q+NS W+ M E+WG+ W
Sbjct: 585 LIEYEDGDGDLRAVYIKQANSNTWQGMEETWGANW 689
Score = 48.3 bits (99), Expect(4) = 7e-045
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -3 / +3
Query: 832 QYPFSSMTSCGNEPIFKDGKGCGSCYQIRCLKS 734
Q PF S + G+ +FK G GCG+CYQ++C ++
Sbjct: 231 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTEN 329
Score = 36.3 bits (73), Expect(4) = 7e-045
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -3 / +3
Query: 955 QDRSLLSYSSGWLPAKATWYGAPTGAG 875
+ L + + W PA ATWYG+P G G
Sbjct: 111 EKHDLATQGNDWQPATATWYGSPDGDG 191
Score = 34.5 bits (69), Expect(4) = 7e-045
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = -3 / +3
Query: 322 SGRQLVAKNVIPANYIPDVDYRSFV 248
+GR L A NVIP N+ P YRS V
Sbjct: 747 TGRTLTANNVIPQNWQPGATYRSLV 821
>gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_9_H09_A029 5', mRNA sequence
Length = 806
Score = 118 bits (253), Expect(3) = 6e-040
Identities = 46/95 (48%), Positives = 63/95 (66%)
Frame = -3 / +2
Query: 664 KYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAV 485
K HFDLSGTAF MAS G + L + G++ + FRRVPC +PG I F V G++ + A+
Sbjct: 434 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 613
Query: 484 LIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIW 380
LIE+E+ DGD+ I Q+NS W+ M E+WG+ W
Sbjct: 614 LIEYEDGDGDLRAVYIKQANSNTWQGMEETWGANW 718
Score = 48.3 bits (99), Expect(3) = 6e-040
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -3 / +2
Query: 832 QYPFSSMTSCGNEPIFKDGKGCGSCYQIRCLKS 734
Q PF S + G+ +FK G GCG+CYQ++C ++
Sbjct: 260 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTEN 358
Score = 36.3 bits (73), Expect(3) = 6e-040
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -3 / +2
Query: 955 QDRSLLSYSSGWLPAKATWYGAPTGAG 875
+ L + + W PA ATWYG+P G G
Sbjct: 140 EKHDLATQGNDWQPATATWYGSPDGDG 220
>gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 3', mRNA sequence
Length = 793
Score = 118 bits (253), Expect(3) = 6e-040
Identities = 46/95 (48%), Positives = 63/95 (66%)
Frame = -3 / -1
Query: 664 KYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAV 485
K HFDLSGTAF MAS G + L + G++ + FRRVPC +PG I F V G++ + A+
Sbjct: 346 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 167
Query: 484 LIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIW 380
LIE+E+ DGD+ I Q+NS W+ M E+WG+ W
Sbjct: 166 LIEYEDGDGDLRAVYIKQANSNTWQGMEETWGANW 62
Score = 48.3 bits (99), Expect(3) = 6e-040
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -3 / -1
Query: 832 QYPFSSMTSCGNEPIFKDGKGCGSCYQIRCLKS 734
Q PF S + G+ +FK G GCG+CYQ++C ++
Sbjct: 520 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTEN 422
Score = 36.3 bits (73), Expect(3) = 6e-040
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -3 / -1
Query: 955 QDRSLLSYSSGWLPAKATWYGAPTGAG 875
+ L + + W PA ATWYG+P G G
Sbjct: 640 EKHDLATQGNDWQPATATWYGSPDGDG 560
>gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_8_F09_A029 5', mRNA sequence
Length = 717
Score = 117 bits (251), Expect(3) = 1e-039
Identities = 46/95 (48%), Positives = 62/95 (65%)
Frame = -3 / +3
Query: 664 KYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAV 485
K HFDLSGTAF MAS G + L + G++ + FRRVPC +PG I F V G++ + A+
Sbjct: 405 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 584
Query: 484 LIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIW 380
LIE+E+ DGD+ I Q+NS W+ M E+WG W
Sbjct: 585 LIEYEDGDGDLRAVYIKQANSNTWQGMEETWGCNW 689
Score = 48.3 bits (99), Expect(3) = 1e-039
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = -3 / +3
Query: 832 QYPFSSMTSCGNEPIFKDGKGCGSCYQIRCLKS 734
Q PF S + G+ +FK G GCG+CYQ++C ++
Sbjct: 231 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTEN 329
Score = 36.3 bits (73), Expect(3) = 1e-039
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -3 / +3
Query: 955 QDRSLLSYSSGWLPAKATWYGAPTGAG 875
+ L + + W PA ATWYG+P G G
Sbjct: 111 EKHDLATQGNDWQPATATWYGSPDGDG 191
>gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 5', mRNA sequence
Length = 707
Score = 118 bits (253), Expect(2) = 2e-031
Identities = 46/95 (48%), Positives = 63/95 (66%)
Frame = -3 / -2
Query: 664 KYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAV 485
K HFDLSGTAF MAS G + L + G++ + FRRVPC +PG I F V G++ + A+
Sbjct: 703 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 524
Query: 484 LIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIW 380
LIE+E+ DGD+ I Q+NS W+ M E+WG+ W
Sbjct: 523 LIEYEDGDGDLRAVYIKQANSNTWQGMEETWGANW 419
Score = 38.2 bits (77), Expect(2) = 2e-031
Identities = 17/30 (56%), Positives = 19/30 (63%)
Frame = -3 / -2
Query: 322 SGRQLVAKNVIPANYIPDVDYRSFVQY**S 233
+GR L A NVIP N+ P YRS V Y *S
Sbjct: 361 TGRTLTANNVIPQNWQPGATYRSLVNYH*S 272
>gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_29_B05_A029 5', mRNA sequence
Length = 619
Score = 84.5 bits (178), Expect(3) = 2e-030
Identities = 33/68 (48%), Positives = 46/68 (67%)
Frame = -3 / +1
Query: 664 KYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAV 485
K HFDLSGT+F MAS G + +AGI+ + FRRVPC +PG I F V G++ + A+
Sbjct: 415 KIHFDLSGTSFTDMASPGTSSDFLNAGIVSILFRRVPCEYPGNNIAFHVNEGASDHWFAI 594
Query: 484 LIEHENKD 461
L+E+E+ D
Sbjct: 595 LVEYEDGD 618
Score = 48.7 bits (100), Expect(3) = 2e-030
Identities = 14/32 (43%), Positives = 24/32 (75%)
Frame = -3 / +1
Query: 826 PFSSMTSCGNEPIFKDGKGCGSCYQIRCLKSN 731
PF S + G+ +F+ GKGCG+CYQ++C +++
Sbjct: 247 PFQSKVAAGSPALFEGGKGCGACYQVKCTENS 342
Score = 38.2 bits (77), Expect(3) = 2e-030
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -3 / +1
Query: 943 LLSYSSGWLPAKATWYGAPTGAG 875
L+ S W PA ATWYG+P G G
Sbjct: 133 LVEVGSNWQPATATWYGSPDGDG 201
>gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST17B02, mRNA sequence
Length = 598
Score = 112 bits (239), Expect(2) = 2e-029
Identities = 47/126 (37%), Positives = 72/126 (57%)
Frame = -3 / +2
Query: 679 YYPVAKYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNP 500
Y + HFDLSG AFG MA+ G L ++G + + +RR C +PG ITF V GS
Sbjct: 122 YCAFGRTHFDLSGAAFGHMATNGKTPALLNSGELPVLYRRTLCEYPGKNITFHVTEGSTA 301
Query: 499 MYLAVLIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIWRIDPNRPLRGPFSMRITNES 320
+ ++LI +E+ DGDV + Q+ S W M WG+ W + N L+ PFS+R+T +
Sbjct: 302 YWFSILIGYEDGDGDVGAMHLKQAESDAWMEMRHVWGANWCLLGNDSLKXPFSVRVTTLT 481
Query: 319 GRQLVA 302
++ ++
Sbjct: 482 SKRTLS 499
Score = 37.7 bits (76), Expect(2) = 2e-029
Identities = 9/18 (50%), Positives = 17/18 (94%)
Frame = -3 / +2
Query: 790 IFKDGKGCGSCYQIRCLK 737
+FK G+GCG+CY+++C++
Sbjct: 8 VFKGGEGCGACYKVKCME 61
>gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_022_D05 5' similar to Arabidopsis
thaliana sequence At4g28250 putative
beta-expansin/allergen protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 458
Score = 112 bits (240), Expect = 4e-024
Identities = 46/119 (38%), Positives = 70/119 (58%)
Frame = -3 / +1
Query: 658 HFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAVLI 479
HFDLSG AFG MA+ G L + G + + +RR C +PG ITF V GS + ++LI
Sbjct: 13 HFDLSGAAFGRMATNGKTPALLNTGELPVLYRRTLCEYPGKNITFHVTEGSTAYWFSILI 192
Query: 478 EHENKDGDVVQADIMQSNSGHWEPMHESWGSIWRIDPNRPLRGPFSMRITNESGRQLVA 302
E+E+ DGDV + Q++S W M+ WG+ W + PL+ PFS +T + ++ ++
Sbjct: 193 EYEDGDGDVGAMHLKQADSDAWMEMNHVWGANWCLLGGTPLKAPFSXXVTTLTSKRTLS 369
>gb|DR387045.1|DR387045 RTHG1_19_C01.b1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 3', mRNA sequence
Length = 725
Score = 87.7 bits (185), Expect(2) = 3e-022
Identities = 31/71 (43%), Positives = 47/71 (66%)
Frame = -3 / +2
Query: 592 HAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAVLIEHENKDGDVVQADIMQSNSGHW 413
+ G++ + FRRVPC +PG I F V G++ + A+LIE+E+ DGD+ I Q+NS W
Sbjct: 5 NTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILIEYEDGDGDLRAVYIKQANSNTW 184
Query: 412 EPMHESWGSIW 380
+ M E+WG+ W
Sbjct: 185 QGMEETWGANW 217
Score = 38.2 bits (77), Expect(2) = 3e-022
Identities = 17/30 (56%), Positives = 19/30 (63%)
Frame = -3 / +2
Query: 322 SGRQLVAKNVIPANYIPDVDYRSFVQY**S 233
+GR L A NVIP N+ P YRS V Y *S
Sbjct: 275 TGRTLTANNVIPQNWQPGATYRSLVNYH*S 364
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 488,162,080
Number of Sequences: 355925
Number of extensions: 6935944
Number of successful extensions: 72827
Number of sequences better than 1.0e-020: 12
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 17728929320
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)