TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3276099.2.1
         (545 letters)

Database: Pinus_nucl_with_EST.fasta 
           355,925 sequences; 217,277,237 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DT631572.1|DT631572  EST1146503 Normalized pine embryo li...    77   4e-024
gb|DR683340.1|DR683340  EST1073416 Normalized pine embryo li...    77   4e-024

>gb|DT631572.1|DT631572 EST1146503 Normalized pine embryo library, Lib_D Pinus taeda cDNA
           clone PIMFA26 3' end, mRNA sequence
          Length = 910

 Score = 77.1 bits (162), Expect(2) = 4e-024
 Identities = 30/47 (63%), Positives = 40/47 (85%)
 Frame = +3 / +2

                                                          
Query: 144 MVVVEANHICMISRGIEKIRSNTATIAVLGQFLADPSAKACFLQNVL 284
           MVV+EA+HICM+SRGIEKI SNTAT+ VLG+F+ D +AKA F + ++
Sbjct: 416 MVVLEASHICMLSRGIEKIGSNTATVTVLGRFVTDSAAKAAFFKKIV 556




 Score = 53.8 bits (111), Expect(2) = 4e-024
 Identities = 23/51 (45%), Positives = 34/51 (66%)
 Frame = +3 / +2

                                                              
Query: 3   FDNGSGEGIDRSHFQALVHFYGCKLQVQERMTRQIAEAVYSVSHNGAMVVV 155
           F     E +DRS   ++V F+  KLQVQER+T+QIAEA++S  +   ++VV
Sbjct: 272 FSVTENEELDRSKINSIVQFFSQKLQVQERLTKQIAEAIHSACNTVDVMVV 424



>gb|DR683340.1|DR683340 EST1073416 Normalized pine embryo library, Lib_D Pinus taeda cDNA
           clone PWAAG06 3' end, mRNA sequence
          Length = 777

 Score = 77.1 bits (162), Expect(2) = 4e-024
 Identities = 30/47 (63%), Positives = 40/47 (85%)
 Frame = +3 / +3

                                                          
Query: 144 MVVVEANHICMISRGIEKIRSNTATIAVLGQFLADPSAKACFLQNVL 284
           MVV+EA+HICM+SRGIEKI SNTAT+ VLG+F+ D +AKA F + ++
Sbjct: 408 MVVLEASHICMLSRGIEKIGSNTATVTVLGRFVTDSAAKAAFFKKIV 548




 Score = 53.8 bits (111), Expect(2) = 4e-024
 Identities = 23/51 (45%), Positives = 34/51 (66%)
 Frame = +3 / +3

                                                              
Query: 3   FDNGSGEGIDRSHFQALVHFYGCKLQVQERMTRQIAEAVYSVSHNGAMVVV 155
           F     E +DRS   ++V F+  KLQVQER+T+QIAEA++S  +   ++VV
Sbjct: 264 FSVTENEELDRSKINSIVQFFSQKLQVQERLTKQIAEAIHSACNTVDVMVV 416



  Database: Pinus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:45 PM
  Number of letters in database: 217,277,237
  Number of sequences in database:  355,925
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 322,905,337
Number of Sequences: 355925
Number of extensions: 4791156
Number of successful extensions: 63894
Number of sequences better than 1.0e-020: 2
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 7055564100
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)