TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3106097.2.2
(1005 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR057730.1|DR057730 RTNIT1_7_B04.g1_A029 Roots minus nit... 152 5e-043
gb|CO366556.1|CO366556 RTK1_28_C09.g1_A029 Roots minus pota... 152 5e-043
gb|DR057381.1|DR057381 RTNIT1_5_B04.g1_A029 Roots minus nit... 152 5e-043
gb|DR110696.1|DR110696 RTS1_12_H11.b1_A029 Roots minus sulf... 152 5e-040
gb|DR110783.1|DR110783 RTS1_12_H11.g1_A029 Roots minus sulf... 152 3e-036
gb|CO197674.1|CO197674 GEO1_8_E04.b1_A029 Root gravitropism... 152 4e-036
gb|DR687633.1|DR687633 EST1077715 Normalized pine embryo li... 146 3e-034
gb|DT634393.1|DT634393 EST1149324 Normalized pine embryo li... 146 3e-034
gb|DR089646.1|DR089646 RTAL1_10_B02.b1_A029 Roots plus adde... 136 3e-033
gb|DR168855.1|DR168855 RTPHOS1_28_G05.b1_A029 Roots minus p... 132 4e-030
gb|CX715653.1|CX715653 RTPQ1_35_D08.g1_A032 Roots treated w... 130 2e-029
gb|CX648692.1|CX648692 COLD1_30_F12.b1_A029 Root cold Pinus... 118 5e-026
gb|CX651090.1|CX651090 COLD1_50_C05.b1_A029 Root cold Pinus... 114 9e-025
gb|DR168933.1|DR168933 RTPHOS1_28_G05.g1_A029 Roots minus p... 83 3e-022
gb|CX651163.1|CX651163 COLD1_50_C05.g1_A029 Root cold Pinus... 102 5e-021
gb|CX648762.1|CX648762 COLD1_30_F12.g1_A029 Root cold Pinus... 101 9e-021
>gb|DR057730.1|DR057730 RTNIT1_7_B04.g1_A029 Roots minus nitrogen Pinus taeda cDNA clone
RTNIT1_7_B04_A029 5', mRNA sequence
Length = 929
Score = 152 bits (327), Expect(2) = 5e-043
Identities = 56/108 (51%), Positives = 85/108 (78%)
Frame = +3 / +3
Query: 219 KIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLA 398
+ ED E S+ +C++++ N + AP E+RR +R++ LRCHV+Y+ + S+L V+P+ A
Sbjct: 219 RFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHA 398
Query: 399 SRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGS 542
SRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+ILKG+
Sbjct: 399 SRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLILKGT 542
Score = 42.8 bits (87), Expect(2) = 5e-043
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +3 / +3
Query: 105 PFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKN 203
P D DY S I+YRWG EGLRK++ + +N
Sbjct: 117 PIYPDPDYDSHIDYRWGAEGLRKSMKKLRGPEN 215
>gb|CO366556.1|CO366556 RTK1_28_C09.g1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_28_C09_A029 5', mRNA sequence
Length = 851
Score = 152 bits (327), Expect(2) = 5e-043
Identities = 56/108 (51%), Positives = 85/108 (78%)
Frame = +3 / -2
Query: 219 KIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLA 398
+ ED E S+ +C++++ N + AP E+RR +R++ LRCHV+Y+ + S+L V+P+ A
Sbjct: 664 RFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHA 485
Query: 399 SRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGS 542
SRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+ILKG+
Sbjct: 484 SRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLILKGT 341
Score = 42.8 bits (87), Expect(2) = 5e-043
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +3 / -2
Query: 105 PFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKN 203
P D DY S I+YRWG EGLRK++ + +N
Sbjct: 766 PIYPDPDYDSHIDYRWGAEGLRKSMKKLRGPEN 668
>gb|DR057381.1|DR057381 RTNIT1_5_B04.g1_A029 Roots minus nitrogen Pinus taeda cDNA clone
RTNIT1_5_B04_A029 5', mRNA sequence
Length = 829
Score = 152 bits (327), Expect(2) = 5e-043
Identities = 56/108 (51%), Positives = 85/108 (78%)
Frame = +3 / +3
Query: 219 KIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLA 398
+ ED E S+ +C++++ N + AP E+RR +R++ LRCHV+Y+ + S+L V+P+ A
Sbjct: 225 RFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHA 404
Query: 399 SRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGS 542
SRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+ILKG+
Sbjct: 405 SRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLILKGT 548
Score = 42.8 bits (87), Expect(2) = 5e-043
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +3 / +3
Query: 105 PFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKN 203
P D DY S I+YRWG EGLRK++ + +N
Sbjct: 123 PIYPDPDYDSHIDYRWGAEGLRKSMKKLRGPEN 221
>gb|DR110696.1|DR110696 RTS1_12_H11.b1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_12_H11_A029 3', mRNA sequence
Length = 763
Score = 152 bits (327), Expect(2) = 5e-040
Identities = 56/108 (51%), Positives = 85/108 (78%)
Frame = +3 / +1
Query: 219 KIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLA 398
+ ED E S+ +C++++ N + AP E+RR +R++ LRCHV+Y+ + S+L V+P+ A
Sbjct: 70 RFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHA 249
Query: 399 SRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGS 542
SRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+ILKG+
Sbjct: 250 SRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLILKGT 393
Score = 32.7 bits (65), Expect(2) = 5e-040
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +3 / +1
Query: 138 IEYRWGGEGLRKTLIRWAAEKN 203
I+YRWG EGLRK++ + +N
Sbjct: 1 IDYRWGAEGLRKSMKKLRGPEN 66
>gb|DR110783.1|DR110783 RTS1_12_H11.g1_A029 Roots minus sulfur Pinus taeda cDNA clone
RTS1_12_H11_A029 5', mRNA sequence
Length = 759
Score = 152 bits (327), Expect = 3e-036
Identities = 56/108 (51%), Positives = 85/108 (78%)
Frame = +3 / +2
Query: 219 KIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLA 398
+ ED E S+ +C++++ N + AP E+RR +R++ LRCHV+Y+ + S+L V+P+ A
Sbjct: 32 RFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHA 211
Query: 399 SRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGS 542
SRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+ILKG+
Sbjct: 212 SRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLILKGT 355
>gb|CO197674.1|CO197674 GEO1_8_E04.b1_A029 Root gravitropism April 2003 test Pinus taeda
cDNA clone GEO1_8_E04_A029 3', mRNA sequence
Length = 669
Score = 152 bits (326), Expect = 4e-036
Identities = 56/104 (53%), Positives = 84/104 (80%)
Frame = +3 / +3
Query: 231 EDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQ 410
ED E S+ +C++++ N + AP E+RR +R++ LRCHV+Y+ + S+L V+P+ ASRSQ
Sbjct: 9 EDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHASRSQ 188
Query: 411 ALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGS 542
ALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+ILKG+
Sbjct: 189 ALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLILKGT 320
>gb|DR687633.1|DR687633 EST1077715 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABU74 3' end, mRNA sequence
Length = 797
Score = 146 bits (313), Expect = 3e-034
Identities = 57/142 (40%), Positives = 95/142 (66%)
Frame = +3 / +2
Query: 117 DLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQKIFIEDEECSSTYCISFKGSNTAAAP 296
D D H + G +L+ + +E+ +++ ED + S+ +C+++ ++
Sbjct: 194 DYDSHIDYRWGGEGLRKTMSLLTASERDGQEKQERVIFEDAKHSNAHCLAYVVKDSTRVR 373
Query: 297 PVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGE 476
V E R+ +R++ LRCH ++ + ++L+VIP+LASRSQALRYL++RWG++++N+ V VGE
Sbjct: 374 KVDEFRQRLRMRGLRCHPMFCRNSTQLHVIPLLASRSQALRYLFVRWGLDVANMHVFVGE 553
Query: 477 CGDTDYEGLLGGVHKTIILKGS 542
GDTDYE +L G+HKTIILKG+
Sbjct: 554 TGDTDYEEMLAGLHKTIILKGA 619
>gb|DT634393.1|DT634393 EST1149324 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMG549 3' end, mRNA sequence
Length = 827
Score = 146 bits (313), Expect = 3e-034
Identities = 57/142 (40%), Positives = 95/142 (66%)
Frame = +3 / +1
Query: 117 DLDYHSQIEYRWGGEGLRKTLIRWAAEKNKERGQKIFIEDEECSSTYCISFKGSNTAAAP 296
D D H + G +L+ + +E+ +++ ED + S+ +C+++ ++
Sbjct: 202 DYDSHIDYRWGGEGLRKTMSLLTASERDGQEKQERVIFEDAKHSNAHCLAYVVKDSTRVR 381
Query: 297 PVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGE 476
V E R+ +R++ LRCH ++ + ++L+VIP+LASRSQALRYL++RWG++++N+ V VGE
Sbjct: 382 KVDEFRQRLRMRGLRCHPMFCRNSTQLHVIPLLASRSQALRYLFVRWGLDVANMHVFVGE 561
Query: 477 CGDTDYEGLLGGVHKTIILKGS 542
GDTDYE +L G+HKTIILKG+
Sbjct: 562 TGDTDYEEMLAGLHKTIILKGA 627
>gb|DR089646.1|DR089646 RTAL1_10_B02.b1_A029 Roots plus added aluminum Pinus taeda cDNA
clone RTAL1_10_B02_A029 3', mRNA sequence
Length = 882
Score = 136 bits (291), Expect(2) = 3e-033
Identities = 52/76 (68%), Positives = 66/76 (86%)
Frame = +3 / +3
Query: 315 RTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDY 494
+ +R+Q RCHV+Y H GSKL+VIP+LASRSQALRYL+ RWG+E++N+ V +GE GDTDY
Sbjct: 3 KQLRMQGFRCHVIYCHKGSKLHVIPLLASRSQALRYLFTRWGMEVTNMFVFIGETGDTDY 182
Query: 495 EGLLGGVHKTIILKGS 542
E LLGG+HKTIILKG+
Sbjct: 183 EELLGGLHKTIILKGT 230
Score = 26.3 bits (51), Expect(2) = 3e-033
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +2 / +2
Query: 557 KPSSC*QKLFIPRCCIL*QTRNCFN*G 637
KPSS Q L RCC *Q +C +*G
Sbjct: 248 KPSS*QQ*LCKRRCCAT*QPEHCDD*G 328
>gb|DR168855.1|DR168855 RTPHOS1_28_G05.b1_A029 Roots minus phosphorous Pinus taeda cDNA
clone RTPHOS1_28_G05_A029 3', mRNA sequence
Length = 763
Score = 132 bits (283), Expect = 4e-030
Identities = 49/83 (59%), Positives = 70/83 (84%)
Frame = +3 / +2
Query: 294 PPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVG 473
P E+RR +R++ LRCHV+Y+ + S+L V+P+ ASRSQALRYLY+RWG++++N+ V +G
Sbjct: 2 PKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHASRSQALRYLYVRWGLDIANMYVFLG 181
Query: 474 ECGDTDYEGLLGGVHKTIILKGS 542
E GDTDYE L+GG+HKT+ILKG+
Sbjct: 182 EKGDTDYEELIGGMHKTLILKGT 250
>gb|CX715653.1|CX715653 RTPQ1_35_D08.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_35_D08_A032 5', mRNA sequence
Length = 603
Score = 130 bits (278), Expect = 2e-029
Identities = 48/79 (60%), Positives = 69/79 (87%)
Frame = +3 / +1
Query: 306 EIRRTMRIQALRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGD 485
E+RR +R++ LRCHV+Y+ + S+L V+P+ ASRSQALRYLY+RWG++++N+ V +GE GD
Sbjct: 16 ELRRQLRMRGLRCHVIYTQNCSRLRVLPLHASRSQALRYLYVRWGLDIANMYVFLGEKGD 195
Query: 486 TDYEGLLGGVHKTIILKGS 542
TDYE L+GG+HKT+ILKG+
Sbjct: 196 TDYEELIGGMHKTLILKGT 252
>gb|CX648692.1|CX648692 COLD1_30_F12.b1_A029 Root cold Pinus taeda cDNA clone
COLD1_30_F12_A029 3', mRNA sequence
Length = 705
Score = 118 bits (253), Expect = 5e-026
Identities = 44/69 (63%), Positives = 61/69 (88%)
Frame = +3 / +2
Query: 336 LRCHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGV 515
LRCHV+Y+ + S+L V+P+ ASRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+
Sbjct: 2 LRCHVIYTQNCSRLRVLPLHASRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGM 181
Query: 516 HKTIILKGS 542
HKT+ILKG+
Sbjct: 182 HKTLILKGT 208
>gb|CX651090.1|CX651090 COLD1_50_C05.b1_A029 Root cold Pinus taeda cDNA clone
COLD1_50_C05_A029 3', mRNA sequence
Length = 699
Score = 114 bits (244), Expect = 9e-025
Identities = 42/67 (62%), Positives = 59/67 (88%)
Frame = +3 / +3
Query: 342 CHVLYSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHK 521
CHV+Y+ + S+L V+P+ ASRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HK
Sbjct: 3 CHVIYTQNCSRLRVLPLHASRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHK 182
Query: 522 TIILKGS 542
T+ILKG+
Sbjct: 183 TLILKGT 203
>gb|DR168933.1|DR168933 RTPHOS1_28_G05.g1_A029 Roots minus phosphorous Pinus taeda cDNA
clone RTPHOS1_28_G05_A029 5', mRNA sequence
Length = 537
Score = 83.1 bits (175), Expect(2) = 3e-022
Identities = 31/68 (45%), Positives = 49/68 (72%)
Frame = +3 / +1
Query: 219 KIFIEDEECSSTYCISFKGSNTAAAPPVKEIRRTMRIQALRCHVLYSHDGSKLNVIPVLA 398
+ ED E S+ +C++++ N + AP E+RR +R++ LRCHV+Y+ + S+L V+P+ A
Sbjct: 325 RFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQNCSRLRVLPLHA 504
Query: 399 SRSQALRY 422
SRSQALRY
Sbjct: 505 SRSQALRY 528
Score = 42.8 bits (87), Expect(2) = 3e-022
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +3 / +1
Query: 105 PFMIDLDYHSQIEYRWGGEGLRKTLIRWAAEKN 203
P D DY S I+YRWG EGLRK++ + +N
Sbjct: 223 PIYPDPDYDSHIDYRWGAEGLRKSMKKLRGPEN 321
>gb|CX651163.1|CX651163 COLD1_50_C05.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_50_C05_A029 5', mRNA sequence
Length = 608
Score = 102 bits (217), Expect = 5e-021
Identities = 38/63 (60%), Positives = 55/63 (87%)
Frame = +3 / +2
Query: 354 YSHDGSKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIIL 533
++ + S+L V+P+ ASRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+IL
Sbjct: 8 FTQNCSRLRVLPLHASRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLIL 187
Query: 534 KGS 542
KG+
Sbjct: 188 KGT 196
>gb|CX648762.1|CX648762 COLD1_30_F12.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_30_F12_A029 5', mRNA sequence
Length = 659
Score = 101 bits (215), Expect = 9e-021
Identities = 38/58 (65%), Positives = 52/58 (89%)
Frame = +3 / +2
Query: 369 SKLNVIPVLASRSQALRYLYIRWGVELSNITVIVGECGDTDYEGLLGGVHKTIILKGS 542
S+L V+P+ ASRSQALRYLY+RWG++++N+ V +GE GDTDYE L+GG+HKT+ILKG+
Sbjct: 8 SRLRVLPLHASRSQALRYLYVRWGLDIANMYVFLGEKGDTDYEELIGGMHKTLILKGT 181
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 497,925,076
Number of Sequences: 355925
Number of extensions: 7133809
Number of successful extensions: 29797
Number of sequences better than 1.0e-020: 16
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 15050843320
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)