TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3012915.2.2
(621 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus adde... 118 7e-034
gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus adde... 118 2e-032
gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip libra... 122 8e-032
gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compr... 120 1e-030
gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinu... 118 3e-026
gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus adde... 118 3e-026
gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added... 117 5e-026
gb|DR387045.1|DR387045 RTHG1_19_C01.b1_A029 Roots plus adde... 91 8e-026
gb|DR385374.1|DR385374 RTHG1_8_F09.b1_A029 Roots plus added... 85 5e-024
gb|DR024403.1|DR024403 STRS1_64_E12.g1_A034 Shoot tip pitch... 91 3e-023
gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus adde... 102 2e-021
>gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 5', mRNA sequence
Length = 707
Score = 118 bits (252), Expect(2) = 7e-034
Identities = 42/97 (43%), Positives = 65/97 (67%)
Frame = +2 / -2
Query: 104 HFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLV 283
HFDLSGTAF +A G + +L G++ + F RVPCE+PG + F+V G++ +FA+L+
Sbjct: 697 HFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILI 518
Query: 284 EYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRLDS 394
EYE+GDGD+ V + + + W M ++WG+ W L+S
Sbjct: 517 EYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNS 407
Score = 45.5 bits (93), Expect(2) = 7e-034
Identities = 19/45 (42%), Positives = 27/45 (60%)
Frame = +2 / -2
Query: 440 SGKTLVARDVIPLNWRPNTFYRSIVQYSS*IPQFISCSYSCSLFV 574
+G+TL A +VIP NW+P YRS+V Y + + C+ S FV
Sbjct: 361 TGRTLTANNVIPQNWQPGATYRSLVNYH*SPSELL*CAISFVAFV 227
>gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 5', mRNA sequence
Length = 821
Score = 118 bits (253), Expect(2) = 2e-032
Identities = 43/102 (42%), Positives = 66/102 (64%)
Frame = +2 / +3
Query: 89 GVAPYHFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVY 268
G HFDLSGTAF +A G + +L G++ + F RVPCE+PG + F+V G++ +
Sbjct: 396 GSGKKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYW 575
Query: 269 FAVLVEYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRLDS 394
FA+L+EYE+GDGD+ V + + + W M ++WG+ W L+S
Sbjct: 576 FAILIEYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNS 701
Score = 40.0 bits (81), Expect(2) = 2e-032
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +2 / +3
Query: 440 SGKTLVARDVIPLNWRPNTFYRSIV 514
+G+TL A +VIP NW+P YRS+V
Sbjct: 747 TGRTLTANNVIPQNWQPGATYRSLV 821
>gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST17B02, mRNA sequence
Length = 598
Score = 122 bits (262), Expect(2) = 8e-032
Identities = 49/115 (42%), Positives = 69/115 (60%)
Frame = +2 / +2
Query: 104 HFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLV 283
HFDLSG AFG +A G+ L +G + + + R CE+PG + FHV EGST +F++L+
Sbjct: 143 HFDLSGAAFGHMATNGKTPALLNSGELPVLYRRTLCEYPGKNITFHVTEGSTAYWFSILI 322
Query: 284 EYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRLDSNHRLQPPFSIRTRTDSGK 448
YE+GDGDV + L + + W M WG+ W L N L+ PFS+R T + K
Sbjct: 323 GYEDGDGDVGAMHLKQAESDAWMEMRHVWGANWCLLGNDSLKXPFSVRVTTLTSK 487
Score = 34.1 bits (68), Expect(2) = 8e-032
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +2 / +2
Query: 428 TRTDSGKTLVARDVIPLNWRPNTFYRSIVQYSS* 529
T S +TL AR+VIP +W PN Y++ + + *
Sbjct: 470 TTLTSKRTLSARNVIPEHWYPNATYKARLNFDD* 571
>gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_022_D05 5' similar to Arabidopsis
thaliana sequence At4g28250 putative
beta-expansin/allergen protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 458
Score = 120 bits (256), Expect(2) = 1e-030
Identities = 49/115 (42%), Positives = 68/115 (59%)
Frame = +2 / +1
Query: 104 HFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLV 283
HFDLSG AFGR+A G+ L G + + + R CE+PG + FHV EGST +F++L+
Sbjct: 13 HFDLSGAAFGRMATNGKTPALLNTGELPVLYRRTLCEYPGKNITFHVTEGSTAYWFSILI 192
Query: 284 EYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRLDSNHRLQPPFSIRTRTDSGK 448
EYE+GDGDV + L + + W M+ WG+ W L L+ PFS T + K
Sbjct: 193 EYEDGDGDVGAMHLKQADSDAWMEMNHVWGANWCLLGGTPLKAPFSXXVTTLTSK 357
Score = 33.1 bits (66), Expect(2) = 1e-030
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = +2 / +1
Query: 428 TRTDSGKTLVARDVIPLNWRPNTFYRSIVQY 520
T S +TL AR+VIP +W PN Y++ + +
Sbjct: 340 TTLTSKRTLSARNVIPDHWYPNATYKARLNF 432
>gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_9_H09_A029 5', mRNA sequence
Length = 806
Score = 118 bits (253), Expect = 3e-026
Identities = 43/102 (42%), Positives = 66/102 (64%)
Frame = +2 / +2
Query: 89 GVAPYHFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVY 268
G HFDLSGTAF +A G + +L G++ + F RVPCE+PG + F+V G++ +
Sbjct: 425 GSGKKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYW 604
Query: 269 FAVLVEYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRLDS 394
FA+L+EYE+GDGD+ V + + + W M ++WG+ W L+S
Sbjct: 605 FAILIEYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNS 730
>gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 3', mRNA sequence
Length = 793
Score = 118 bits (253), Expect = 3e-026
Identities = 43/102 (42%), Positives = 66/102 (64%)
Frame = +2 / -1
Query: 89 GVAPYHFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVY 268
G HFDLSGTAF +A G + +L G++ + F RVPCE+PG + F+V G++ +
Sbjct: 355 GSGKKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYW 176
Query: 269 FAVLVEYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRLDS 394
FA+L+EYE+GDGD+ V + + + W M ++WG+ W L+S
Sbjct: 175 FAILIEYEDGDGDLRAVYIKQANSNTWQGMEETWGANWCLNS 50
>gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_8_F09_A029 5', mRNA sequence
Length = 717
Score = 117 bits (251), Expect = 5e-026
Identities = 43/102 (42%), Positives = 65/102 (63%)
Frame = +2 / +3
Query: 89 GVAPYHFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVY 268
G HFDLSGTAF +A G + +L G++ + F RVPCE+PG + F+V G++ +
Sbjct: 396 GSGKKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYW 575
Query: 269 FAVLVEYENGDGDVVQVDLMEKRARRWTPMSQSWGSIWRLDS 394
FA+L+EYE+GDGD+ V + + + W M ++WG W L+S
Sbjct: 576 FAILIEYEDGDGDLRAVYIKQANSNTWQGMEETWGCNWCLNS 701
>gb|DR387045.1|DR387045 RTHG1_19_C01.b1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 3', mRNA sequence
Length = 725
Score = 91.3 bits (193), Expect(2) = 8e-026
Identities = 30/73 (41%), Positives = 50/73 (68%)
Frame = +2 / +2
Query: 176 GIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLVEYENGDGDVVQVDLMEKRARRWTP 355
G++ + F RVPCE+PG + F+V G++ +FA+L+EYE+GDGD+ V + + + W
Sbjct: 11 GVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILIEYEDGDGDLRAVYIKQANSNTWQG 190
Query: 356 MSQSWGSIWRLDS 394
M ++WG+ W L+S
Sbjct: 191 MEETWGANWCLNS 229
Score = 45.5 bits (93), Expect(2) = 8e-026
Identities = 19/45 (42%), Positives = 27/45 (60%)
Frame = +2 / +2
Query: 440 SGKTLVARDVIPLNWRPNTFYRSIVQYSS*IPQFISCSYSCSLFV 574
+G+TL A +VIP NW+P YRS+V Y + + C+ S FV
Sbjct: 275 TGRTLTANNVIPQNWQPGATYRSLVNYH*SPSELL*CAISFVAFV 409
>gb|DR385374.1|DR385374 RTHG1_8_F09.b1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_8_F09_A029 3', mRNA sequence
Length = 695
Score = 85.4 bits (180), Expect(2) = 5e-024
Identities = 29/67 (43%), Positives = 46/67 (68%)
Frame = +2 / +2
Query: 194 FARVPCEFPGLKVGFHVEEGSTQVYFAVLVEYENGDGDVVQVDLMEKRARRWTPMSQSWG 373
F RVPCE+PG + F+V G++ +FA+L+EYE+GDGD+ V + + + W M ++WG
Sbjct: 2 FRRVPCEYPGKNIAFNVNGGASDYWFAILIEYEDGDGDLRAVYIKQANSNTWQGMEETWG 181
Query: 374 SIWRLDS 394
+ W L+S
Sbjct: 182 ANWCLNS 202
Score = 45.5 bits (93), Expect(2) = 5e-024
Identities = 19/45 (42%), Positives = 27/45 (60%)
Frame = +2 / +2
Query: 440 SGKTLVARDVIPLNWRPNTFYRSIVQYSS*IPQFISCSYSCSLFV 574
+G+TL A +VIP NW+P YRS+V Y + + C+ S FV
Sbjct: 248 TGRTLTANNVIPQNWQPGATYRSLVNYH*SPSELL*CAISFVAFV 382
>gb|DR024403.1|DR024403 STRS1_64_E12.g1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_64_E12_A034 5', mRNA sequence
Length = 855
Score = 90.9 bits (192), Expect(2) = 3e-023
Identities = 37/78 (47%), Positives = 47/78 (60%)
Frame = +2 / +2
Query: 155 NDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVYFAVLVEYENGDGDVVQVDLMEK 334
N D +G I + + R CEFP V FHV EGST +F++L+EYE+GDGDV V L E
Sbjct: 20 NHDDSHSGEIPVLYRRTLCEFPRKNVTFHVNEGSTAYWFSILIEYEDGDGDVGAVHLREA 199
Query: 335 RARRWTPMSQSWGSIWRL 388
+ W M SWG+ W L
Sbjct: 200 DSDEWMAMQHSWGANWCL 253
Score = 37.3 bits (75), Expect(2) = 3e-023
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +2 / +2
Query: 428 TRTDSGKTLVARDVIPLNWRPNTFYRSIVQY 520
T S +TL AR++IP NW P YRS + +
Sbjct: 293 TTLSSNRTLSARNIIPQNWYPTASYRSRLNF 385
>gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_29_B05_A029 5', mRNA sequence
Length = 619
Score = 102 bits (218), Expect = 2e-021
Identities = 39/71 (54%), Positives = 50/71 (70%)
Frame = +2 / +1
Query: 89 GVAPYHFDLSGTAFGRLAKPGRNDDLRRAGIIDIQFARVPCEFPGLKVGFHVEEGSTQVY 268
G HFDLSGT+F +A PG + D AGI+ I F RVPCE+PG + FHV EG++ +
Sbjct: 406 GSGKIHFDLSGTSFTDMASPGTSSDFLNAGIVSILFRRVPCEYPGNNIAFHVNEGASDHW 585
Query: 269 FAVLVEYENGD 301
FA+LVEYE+GD
Sbjct: 586 FAILVEYEDGD 618
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 213,758,882
Number of Sequences: 355925
Number of extensions: 2573180
Number of successful extensions: 29059
Number of sequences better than 1.0e-020: 14
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 8412403350
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)