TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2969966.2.1
(1029 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciat... 133 4e-057
gb|BF518211.1|BF518211 NXSI_036_D07_F NXSI (Nsf Xylem Side ... 197 1e-049
gb|CF479164.1|CF479164 RTWW3_24_E11.g1_A022 Well-watered lo... 154 3e-043
gb|DN612931.1|DN612931 EST965981 Subtracted pine embryo lib... 121 9e-042
gb|DT628876.1|DT628876 EST1155446 Subtracted pine embryo li... 121 9e-042
gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo li... 107 2e-041
gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo li... 107 2e-041
>gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP032F08, mRNA sequence
Length = 674
Score = 133 bits (284), Expect(2) = 4e-057
Identities = 56/97 (57%), Positives = 70/97 (72%)
Frame = +3 / +1
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
TGWSVFVS KRLVAGD+VLF+ +E +L LGIR A++ Q+ +PSSVLSS +H G+L
Sbjct: 184 TGWSVFVSYKRLVAGDAVLFLRDENGELRLGIRRASQQQSSVPSSVLSSHGIHSGVLAAV 363
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAVFHT 311
T S F I+YNPR SPTEFVIP KY+K+ H+
Sbjct: 364 AHAVATKSMFHIYYNPRTSPTEFVIPYHKYVKSFNHS 474
Score = 109 bits (233), Expect(2) = 4e-057
Identities = 42/70 (60%), Positives = 51/70 (72%)
Frame = +3 / +1
Query: 303 FHTRISVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSYWRSVKVGWDESTAGER 482
F+ S+GMRF+M FETE+++ RRY GTI + D DP+RWP+S WRS KVGWDE A R
Sbjct: 463 FNHSFSIGMRFKMRFETEDATERRYTGTIVGIGDVDPMRWPNSRWRSFKVGWDEHAAQXR 642
Query: 483 PPRVSLWEIE 512
RVS WEIE
Sbjct: 643 QDRVSPWEIE 672
Score = 84.0 bits (177), Expect(2) = 7e-033
Identities = 48/95 (50%), Positives = 54/95 (56%)
Frame = -3 / -1
Query: 289 YFERGITNSVGLALGL*KIVKRLFVXXXXXXXARSPMCMLSLERTEEGITVCGRVACLIP 110
Y GITNSVGL LGL* + LFV A +P CM LE TEEG +C A IP
Sbjct: 452 YL*YGITNSVGLVLGL**MWNILFVATA*ATAASTPECMPWLESTEEGTLLCCWEALRIP 273
Query: 109 SKSWFFSFHMNSTESPATSLLALTNTLQPVSCRIL 5
+ + FS ST SPATSLL LT TL PV + L
Sbjct: 272 NLNSPFSSRRKSTASPATSLL*LTKTLHPVVSKCL 168
Score = 77.6 bits (163), Expect(2) = 7e-033
Identities = 39/66 (59%), Positives = 43/66 (65%)
Frame = -3 / -1
Query: 514 GSISHRETLGGLSPAVDSSQPTFTDLQ*ELGQRTGSASLTSVIVPMYLRTLDSSVSNNIL 335
GSISH ETL LS A SS PT DL ELG GS S IVP+YL ++ SSVSN IL
Sbjct: 674 GSISHGETLSCLSCAACSSHPTLNDLHLELGHLIGSTSPIPTIVPVYLLSVASSVSNLIL 495
Query: 334 NRIPTD 317
NRIP +
Sbjct: 494 NRIPIE 477
>gb|BF518211.1|BF518211 NXSI_036_D07_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_036_D07 5' similar to Arabidopsis thaliana
sequence At1g30330 putative protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 323
Score = 197 bits (424), Expect = 1e-049
Identities = 81/106 (76%), Positives = 88/106 (83%)
Frame = +3 / +1
Query: 87 NEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXXXXXXXTNSRFTIFYNPRASPTE 266
NEK QLLLGIR A RPQ VMPS VLSSDSMHIGLL TNSRFTIFYNPRASP+E
Sbjct: 1 NEKGQLLLGIRRANRPQAVMPSLVLSSDSMHIGLLAAAAHAAATNSRFTIFYNPRASPSE 180
Query: 267 FVIPLSKYIKAVFHTRISVGMRFRMLFETEESSVRRYMGTITEVSD 404
FVIPL+KY+KAV+HTR+S+GMRF MLFETEE VRRYMGTIT + D
Sbjct: 181 FVIPLAKYVKAVYHTRVSIGMRFXMLFETEEXXVRRYMGTITGIXD 318
Score = 144 bits (309), Expect = 1e-033
Identities = 70/105 (66%), Positives = 77/105 (73%)
Frame = -3 / -1
Query: 403 SLTSVIVPMYLRTLDSSVSNNILNRIPTDIRV*KTALMYFERGITNSVGLALGL*KIVKR 224
SL V+VP+YLRTL SSVSN+I RIP + RV* TAL YF RG+TNS GLALGL*KIV R
Sbjct: 317 SLMPVMVPIYLRTLXSSVSNSIXKRIPIETRV**TALTYFARGMTNSDGLALGL*KIVNR 138
Query: 223 LFVXXXXXXXARSPMCMLSLERTEEGITVCGRVACLIPSKSWFFS 89
LFV ARSP+CMLSLE T EGIT CG A LIP+ S FS
Sbjct: 137 LFVAAA*AAAARSPICMLSLESTNEGITACGLFARLIPNNSCPFS 3
>gb|CF479164.1|CF479164 RTWW3_24_E11.g1_A022 Well-watered loblolly pine roots WW3 Pinus
taeda cDNA clone RTWW3_24_E11_A022 5', mRNA sequence
Length = 714
Score = 154 bits (331), Expect(2) = 3e-043
Identities = 58/76 (76%), Positives = 66/76 (86%)
Frame = +3 / +2
Query: 369 RRYMGTITEVSDADPVRWPSSYWRSVKVGWDESTAGERPPRVSLWEIEPLTTFPMYPSLF 548
RRYMGTI +SD D VRWP+S+WRSVKVGWDESTAGER PRVSLWEIEPLTTFPMY S +
Sbjct: 2 RRYMGTIIGISDLDQVRWPNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTTFPMYSSPY 181
Query: 549 PLRVKHPWYSGLAALH 596
PLR+K PW G+++LH
Sbjct: 182 PLRLKRPWPQGISSLH 229
Score = 41.8 bits (85), Expect(2) = 3e-043
Identities = 20/67 (29%), Positives = 26/67 (38%)
Frame = +3 / +2
Query: 615 MWLRGVTGEGGFHSSNFQSPGIGSWGQQRLHPSLLSNDHDQXXXXXXXXXXXXXXXXLKQ 794
MW+R +G+ + NFQ G+ W QQR SLL D +
Sbjct: 269 MWMRDDSGDRSVQALNFQGMGMNHWMQQRFDSSLLGMQPDVYQAMAAAALQEMRSVDPSK 448
Query: 795 QFLHLQQ 815
Q L QQ
Sbjct: 449 QLLQFQQ 469
>gb|DN612931.1|DN612931 EST965981 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PSABL95, mRNA sequence
Length = 596
Score = 121 bits (259), Expect(2) = 9e-042
Identities = 45/94 (47%), Positives = 68/94 (72%)
Frame = +3 / -1
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
TGWSVFVS+KRLVAGD+ +F+ E +L +G+R A R Q +PSSV+SS SMH+G++
Sbjct: 428 TGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQQNNVPSSVISSHSMHLGVIATA 249
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
T + F+++Y PR SP+EF+IP +Y++++
Sbjct: 248 SHAVTTKTMFSVYYKPRTSPSEFIIPYDQYMESM 147
Score = 79.4 bits (167), Expect(2) = 7e-022
Identities = 43/91 (47%), Positives = 53/91 (58%)
Frame = -3 / +1
Query: 277 GITNSVGLALGL*KIVKRLFVXXXXXXXARSPMCMLSLERTEEGITVCGRVACLIPSKSW 98
GI NS GL LGL* + +FV A +P CML L+ T++G C R+A L P++S
Sbjct: 172 GIMNSDGLVLGL**TLNMVFVVTACDAVAMTPRCML*LDITDDGTLFCWRMARLTPTRSS 351
Query: 97 FFSFHMNSTESPATSLLALTNTLQPVSCRIL 5
FS SPATSLL LT TLQPV + L
Sbjct: 352 PFSPLKKINASPATSLLELTKTLQPVVSKCL 444
Score = 69.8 bits (146), Expect(2) = 9e-042
Identities = 27/44 (61%), Positives = 33/44 (75%)
Frame = +3 / -1
Query: 318 SVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSYWRSVK 449
SVGMRF+M FE EE +R+ GTI +SDADPV WP+S WR +K
Sbjct: 134 SVGMRFKMKFEGEEVPEQRFTGTIVGISDADPVNWPNSKWRCLK 3
Score = 45.1 bits (92), Expect(2) = 7e-022
Identities = 24/45 (53%), Positives = 28/45 (62%)
Frame = -3 / +1
Query: 451 TFTDLQ*ELGQRTGSASLTSVIVPMYLRTLDSSVSNNILNRIPTD 317
T L E GQ TGSASL +VP+ L + SS SN ILN IPT+
Sbjct: 1 TLRHLHFEFGQFTGSASLIPTMVPVNLCSGTSSPSNFILNLIPTE 135
>gb|DT628876.1|DT628876 EST1155446 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PIMD303 5' end, mRNA sequence
Length = 596
Score = 121 bits (259), Expect(2) = 9e-042
Identities = 45/94 (47%), Positives = 68/94 (72%)
Frame = +3 / +1
Query: 21 TGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXX 200
TGWSVFVS+KRLVAGD+ +F+ E +L +G+R A R Q +PSSV+SS SMH+G++
Sbjct: 169 TGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQQNNVPSSVISSHSMHLGVIATA 348
Query: 201 XXXXXTNSRFTIFYNPRASPTEFVIPLSKYIKAV 302
T + F+++Y PR SP+EF+IP +Y++++
Sbjct: 349 SHAVTTKTMFSVYYKPRTSPSEFIIPYDQYMESM 450
Score = 79.4 bits (167), Expect(2) = 7e-022
Identities = 43/91 (47%), Positives = 53/91 (58%)
Frame = -3 / -1
Query: 277 GITNSVGLALGL*KIVKRLFVXXXXXXXARSPMCMLSLERTEEGITVCGRVACLIPSKSW 98
GI NS GL LGL* + +FV A +P CML L+ T++G C R+A L P++S
Sbjct: 425 GIMNSDGLVLGL**TLNMVFVVTACDAVAMTPRCML*LDITDDGTLFCWRMARLTPTRSS 246
Query: 97 FFSFHMNSTESPATSLLALTNTLQPVSCRIL 5
FS SPATSLL LT TLQPV + L
Sbjct: 245 PFSPLKKINASPATSLLELTKTLQPVVSKCL 153
Score = 69.8 bits (146), Expect(2) = 9e-042
Identities = 27/44 (61%), Positives = 33/44 (75%)
Frame = +3 / +1
Query: 318 SVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSYWRSVK 449
SVGMRF+M FE EE +R+ GTI +SDADPV WP+S WR +K
Sbjct: 463 SVGMRFKMKFEGEEVPEQRFTGTIVGISDADPVNWPNSKWRCLK 594
Score = 45.1 bits (92), Expect(2) = 7e-022
Identities = 24/45 (53%), Positives = 28/45 (62%)
Frame = -3 / -1
Query: 451 TFTDLQ*ELGQRTGSASLTSVIVPMYLRTLDSSVSNNILNRIPTD 317
T L E GQ TGSASL +VP+ L + SS SN ILN IPT+
Sbjct: 596 TLRHLHFEFGQFTGSASLIPTMVPVNLCSGTSSPSNFILNLIPTE 462
>gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAB186 3' end, mRNA sequence
Length = 841
Score = 107 bits (228), Expect(2) = 2e-041
Identities = 40/67 (59%), Positives = 49/67 (73%)
Frame = +3 / +2
Query: 315 ISVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSYWRSVKVGWDESTAGERPPRV 494
+S+GMRF+M FETE+++ R Y G IT + DADP RWP S WR +KV WDE A E+ RV
Sbjct: 230 LSIGMRFKMRFETEDTAERSYTGMITAIGDADPARWPGSKWRLLKVEWDEHAANEQQERV 409
Query: 495 SLWEIEP 515
S WEIEP
Sbjct: 410 SAWEIEP 430
Score = 83.1 bits (175), Expect(2) = 2e-041
Identities = 33/70 (47%), Positives = 45/70 (64%)
Frame = +3 / +2
Query: 90 EKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXXXXXXXTNSRFTIFYNPRASPTEF 269
E +L LGIR ATR ++ + SSVLS+ + H+ +L T S F +FYNPR SP EF
Sbjct: 8 ENGELRLGIRRATRQKSGILSSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTSPAEF 187
Query: 270 VIPLSKYIKA 299
+IP KY+K+
Sbjct: 188 IIPYQKYVKS 217
>gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFN79 3' end, mRNA sequence
Length = 783
Score = 107 bits (228), Expect(2) = 2e-041
Identities = 40/67 (59%), Positives = 49/67 (73%)
Frame = +3 / +2
Query: 315 ISVGMRFRMLFETEESSVRRYMGTITEVSDADPVRWPSSYWRSVKVGWDESTAGERPPRV 494
+S+GMRF+M FETE+++ R Y G IT + DADP RWP S WR +KV WDE A E+ RV
Sbjct: 230 LSIGMRFKMRFETEDTAERSYTGMITAIGDADPARWPGSKWRLLKVEWDEHAANEQQERV 409
Query: 495 SLWEIEP 515
S WEIEP
Sbjct: 410 SAWEIEP 430
Score = 83.1 bits (175), Expect(2) = 2e-041
Identities = 33/70 (47%), Positives = 45/70 (64%)
Frame = +3 / +2
Query: 90 EKNQLLLGIRHATRPQTVMPSSVLSSDSMHIGLLXXXXXXXXTNSRFTIFYNPRASPTEF 269
E +L LGIR ATR ++ + SSVLS+ + H+ +L T S F +FYNPR SP EF
Sbjct: 8 ENGELRLGIRRATRQKSGILSSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTSPAEF 187
Query: 270 VIPLSKYIKA 299
+IP KY+K+
Sbjct: 188 IIPYQKYVKS 217
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 542,593,501
Number of Sequences: 355925
Number of extensions: 7990533
Number of successful extensions: 49830
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 15479337080
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)