TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750436.2.1
(656 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR011057.1|DR011057 HEAT1_3_C08.b1_A029 Root at 37 C for... 145 8e-037
gb|DR095417.1|DR095417 STRR1_20_H04.g1_A033 Stem Response R... 144 3e-036
gb|DR011118.1|DR011118 HEAT1_3_C08.g1_A029 Root at 37 C for... 125 1e-030
gb|BX253975.1|BX253975 BX253975 Pinus pinaster differenciat... 125 3e-028
gb|DR055172.1|DR055172 RTCA1_22_B04.b1_A029 Roots minus cal... 122 2e-027
gb|DR385984.1|DR385984 RTHG1_12_D02.b1_A029 Roots plus adde... 122 2e-027
gb|BQ697862.1|BQ697862 NXPV_061_G08_F NXPV (Nsf Xylem Plani... 122 3e-027
gb|DR054232.1|DR054232 RTCA1_16_B11.b1_A029 Roots minus cal... 122 3e-027
gb|DR687852.1|DR687852 EST1077935 Normalized pine embryo li... 114 5e-025
gb|BQ701526.1|BQ701526 NXSI_065_F04_F NXSI (Nsf Xylem Side ... 114 7e-025
>gb|DR011057.1|DR011057 HEAT1_3_C08.b1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_3_C08_A029 3', mRNA sequence
Length = 712
Score = 145 bits (312), Expect(2) = 8e-037
Identities = 59/104 (56%), Positives = 81/104 (77%)
Frame = -1 / -1
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
+E+I+S E +L A+ E G+G +L +HEIQ R DA +E+ERSLLELHQVFLDMAV+
Sbjct: 427 LEKIVSTGESENILQKAIQEQGRGRILETIHEIQERHDAVKEIERSLLELHQVFLDMAVL 248
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE QGE+L++IE +VANAS+YV+ G K+L AK++Q+ SRK +C
Sbjct: 247 VEAQGEQLNNIEYNVANASNYVEHGTKQLYTAKKHQKRSRKWMC 116
Score = 28.1 bits (55), Expect(2) = 8e-037
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = -3 / -3
Query: 369 EKQPQVSLHRHHNPAAPRPACHRANRHQPQEVIE 268
EK+P++ +H HH+ A P + ++ QE+I+
Sbjct: 140 EKKPKMDVHWHHSVADTHPHYCCSYSNKHQEIIK 39
>gb|DR095417.1|DR095417 STRR1_20_H04.g1_A033 Stem Response Resistant Pinus taeda cDNA clone
STRR1_20_H04_A033 5', mRNA sequence
Length = 808
Score = 144 bits (308), Expect(2) = 3e-036
Identities = 58/104 (55%), Positives = 80/104 (76%)
Frame = -1 / -2
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
+E+I+S E +L + E G+G +L +HEIQ R DA +E+ERSLLELHQVFLDMAV+
Sbjct: 591 LEKIVSTGESENILQKVIQEQGRGRILETIHEIQERHDAVKEIERSLLELHQVFLDMAVL 412
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE QGE+L++IE +VANAS+YV+ G K+L AK++Q+ SRK +C
Sbjct: 411 VEAQGEQLNNIEYNVANASNYVEHGTKQLYTAKKHQKRSRKWMC 280
Score = 28.1 bits (55), Expect(2) = 3e-036
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = -3 / -1
Query: 369 EKQPQVSLHRHHNPAAPRPACHRANRHQPQEVIE 268
EK+P++ +H HH+ A P + ++ QE+I+
Sbjct: 304 EKKPKMDVHWHHSVADTHPHYCCSYSNKHQEIIK 203
>gb|DR011118.1|DR011118 HEAT1_3_C08.g1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_3_C08_A029 5', mRNA sequence
Length = 792
Score = 125 bits (267), Expect(2) = 1e-030
Identities = 51/81 (62%), Positives = 67/81 (82%)
Frame = -1 / -3
Query: 587 GAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHVANASHYVQ 408
G +L +HEIQ R DA +E+ERSLLELHQVFLDMAV+VE QGE+L++IE +VANAS+YV+
Sbjct: 508 GRILETIHEIQERHDAVKEIERSLLELHQVFLDMAVLVEAQGEQLNNIEYNVANASNYVE 329
Query: 407 GGNKELGKAKEYQRSSRKCLC 345
G K+L AK++Q+ SRK +C
Sbjct: 328 HGTKQLYTAKKHQKRSRKWMC 266
Score = 28.1 bits (55), Expect(2) = 1e-030
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = -3 / -2
Query: 369 EKQPQVSLHRHHNPAAPRPACHRANRHQPQEVIE 268
EK+P++ +H HH+ A P + ++ QE+I+
Sbjct: 290 EKKPKMDVHWHHSVADTHPHYCCSYSNKHQEIIK 189
>gb|BX253975.1|BX253975 BX253975 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP091F01, mRNA sequence
Length = 522
Score = 125 bits (268), Expect = 3e-028
Identities = 51/104 (49%), Positives = 74/104 (71%)
Frame = -1 / +2
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+R+I E++ A+ E G+G ++ + EIQ R DA R++E+ LL+LHQ+FLDMAV+
Sbjct: 47 IDRLIETGDSEQIFQKAIQEQGRGRIMDTLAEIQERHDAVRDIEKKLLDLHQIFLDMAVL 226
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE+QG+ LD+IE+ V+NA +VQ G L KAK QR+SRK +C
Sbjct: 227 VESQGDMLDNIETQVSNAVDHVQSGTVALQKAKTLQRNSRKWMC 358
>gb|DR055172.1|DR055172 RTCA1_22_B04.b1_A029 Roots minus calcium Pinus taeda cDNA clone
RTCA1_22_B04_A029 3', mRNA sequence
Length = 761
Score = 122 bits (261), Expect = 2e-027
Identities = 48/75 (64%), Positives = 64/75 (85%)
Frame = -1 / +1
Query: 569 VHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHVANASHYVQGGNKEL 390
+HEIQ R DAA+E+E+SLLELHQ+FLDMAV+VE QGE+L+DIE HV +A++YV G K+L
Sbjct: 7 IHEIQERHDAAKEIEKSLLELHQIFLDMAVLVEAQGEQLNDIEHHVIHAANYVDHGTKQL 186
Query: 389 GKAKEYQRSSRKCLC 345
AK+YQR++RK +C
Sbjct: 187 KGAKKYQRNTRKWMC 231
>gb|DR385984.1|DR385984 RTHG1_12_D02.b1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_12_D02_A029 3', mRNA sequence
Length = 764
Score = 122 bits (261), Expect = 2e-027
Identities = 47/104 (45%), Positives = 75/104 (72%)
Frame = -1 / +3
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ II E +L A+ E G+G ++ + EIQ R D +E+E++LLEL Q+FLDMAV+
Sbjct: 66 IDHIIETGESENMLQRAIQEQGRGQIIEVIREIQERHDTVKEIEKNLLEL*QIFLDMAVL 245
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
V+TQG++LD+IE++V A+ +V+GG ++L KA+ Q+++RK C
Sbjct: 246 VQTQGQELDNIEANVGRANSFVEGGTRKLRKARNLQKNTRKWTC 377
>gb|BQ697862.1|BQ697862 NXPV_061_G08_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_061_G08 5' similar to Arabidopsis
thaliana sequence At5g08080 syntaxin-like protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 511
Score = 122 bits (260), Expect = 3e-027
Identities = 50/104 (48%), Positives = 73/104 (70%)
Frame = -1 / +3
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+R+I E++ A+ E G+G ++ + EIQ R DA R++E+ LL+L Q+FLDMAV+
Sbjct: 78 IDRLIETGDSEQIFQKAIQEQGRGRIMDTLAEIQERHDAVRDIEKKLLDLQQIFLDMAVL 257
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE+QG+ LD+IE+ V+NA +VQ G L KAK QR+SRK +C
Sbjct: 258 VESQGDMLDNIETQVSNAVDHVQSGTVALQKAKTLQRNSRKWMC 389
>gb|DR054232.1|DR054232 RTCA1_16_B11.b1_A029 Roots minus calcium Pinus taeda cDNA clone
RTCA1_16_B11_A029 3', mRNA sequence
Length = 805
Score = 122 bits (260), Expect = 3e-027
Identities = 50/104 (48%), Positives = 73/104 (70%)
Frame = -1 / +3
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+R+I E++ A+ E G+G ++ + EIQ R DA R++E+ LL+L Q+FLDMAV+
Sbjct: 84 IDRLIETGDSEQIFQKAIQEQGRGRIMDTLAEIQERHDAVRDIEKKLLDLQQIFLDMAVL 263
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE+QG+ LD+IE+ V+NA +VQ G L KAK QR+SRK +C
Sbjct: 264 VESQGDMLDNIETQVSNAVDHVQSGTVALQKAKTLQRNSRKWMC 395
>gb|DR687852.1|DR687852 EST1077935 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABX34 3' end, mRNA sequence
Length = 837
Score = 114 bits (244), Expect = 5e-025
Identities = 47/97 (48%), Positives = 68/97 (70%)
Frame = -1 / +3
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+R+I E++ A+ E G+G ++ + EIQ R DA R++E+ LL+L Q+FLDMAV+
Sbjct: 546 IDRLIETGDSEQIFQKAIQEQGRGRIMDTLAEIQGRHDAVRDIEKKLLDLQQIFLDMAVL 725
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQR 366
VE+QG+ LD+IE+HV+NA +VQ G L KAK QR
Sbjct: 726 VESQGDMLDNIETHVSNAVDHVQSGTVALQKAKTLQR 836
>gb|BQ701526.1|BQ701526 NXSI_065_F04_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_065_F04 5' similar to Arabidopsis thaliana
sequence At1g61290 similar to syntaxin-related protein
knolle see http://mips.gsf.de/proj/thal/db/index.html,
mRNA sequence
Length = 380
Score = 114 bits (243), Expect = 7e-025
Identities = 47/88 (53%), Positives = 63/88 (71%)
Frame = -1 / +1
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
IE+IIS E L A+ E G+G +L +HEIQ R DAA+ +E+SLLELHQ+FLDMAV+
Sbjct: 115 IEKIISTGESETFLQKAIQEQGRGQILDTIHEIQERHDAAKXIEKSLLELHQIFLDMAVL 294
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKE 393
V E+L+DIE HV +A++YV G K+
Sbjct: 295 VXXXXEQLNDIEHHVIHAANYVXXGTKQ 378
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 246,852,629
Number of Sequences: 355925
Number of extensions: 2981882
Number of successful extensions: 27515
Number of sequences better than 1.0e-020: 11
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 8950329070
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)