TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2649891.2.1
(621 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus adde... 71 1e-022
gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus adde... 71 2e-021
>gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 5', mRNA sequence
Length = 707
Score = 70.7 bits (148), Expect(3) = 1e-022
Identities = 27/65 (41%), Positives = 43/65 (66%)
Frame = -2 / -2
Query: 614 MAKPGQADQLRSAGRLRIQYXRVPCSWNGMDIAFKVDAGSNANYLAVLVEYESGDGELRS 435
MA G + L + G + + + RVPC + G +IAF V+ G++ + A+L+EYE GDG+LR+
Sbjct: 664 MASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILIEYEDGDGDLRA 485
Query: 434 VELMQ 420
V + Q
Sbjct: 484 VYIKQ 470
Score = 48.7 bits (100), Expect(3) = 1e-022
Identities = 17/27 (62%), Positives = 23/27 (85%)
Frame = -2 / -2
Query: 299 SGRTVVASDVIPAGWTPGATYRSVVNF 219
+GRT+ A++VIP W PGATYRS+VN+
Sbjct: 361 TGRTLTANNVIPQNWQPGATYRSLVNY 281
Score = 24.4 bits (47), Expect(3) = 1e-022
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = -2 / -2
Query: 383 MQQSWGXVWRXNSG 342
M+++WG W NSG
Sbjct: 445 MEETWGANWCLNSG 404
>gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 5', mRNA sequence
Length = 821
Score = 70.7 bits (148), Expect(3) = 2e-021
Identities = 27/65 (41%), Positives = 43/65 (66%)
Frame = -2 / +3
Query: 614 MAKPGQADQLRSAGRLRIQYXRVPCSWNGMDIAFKVDAGSNANYLAVLVEYESGDGELRS 435
MA G + L + G + + + RVPC + G +IAF V+ G++ + A+L+EYE GDG+LR+
Sbjct: 444 MASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAILIEYEDGDGDLRA 623
Query: 434 VELMQ 420
V + Q
Sbjct: 624 VYIKQ 638
Score = 44.6 bits (91), Expect(3) = 2e-021
Identities = 16/25 (64%), Positives = 21/25 (84%)
Frame = -2 / +3
Query: 299 SGRTVVASDVIPAGWTPGATYRSVV 225
+GRT+ A++VIP W PGATYRS+V
Sbjct: 747 TGRTLTANNVIPQNWQPGATYRSLV 821
Score = 24.4 bits (47), Expect(3) = 2e-021
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = -2 / +3
Query: 383 MQQSWGXVWRXNSG 342
M+++WG W NSG
Sbjct: 663 MEETWGANWCLNSG 704
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 268,500,661
Number of Sequences: 355925
Number of extensions: 3261255
Number of successful extensions: 41469
Number of sequences better than 1.0e-020: 2
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 8412403350
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)