TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2576887.2.1
(1138 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BX679712.1|BX679712 BX679712 RS Pinus pinaster cDNA clon... 80 3e-037
gb|DT633007.1|DT633007 EST1147938 Normalized pine embryo li... 45 2e-025
gb|DR685771.1|DR685771 EST1075848 Normalized pine embryo li... 45 2e-025
gb|DR023234.1|DR023234 STRS1_56_G08.b1_A034 Shoot tip pitch... 45 2e-025
gb|DR117606.1|DR117606 RTMG1_8_A07.b1_A029 Roots minus magn... 43 3e-025
gb|DR117675.1|DR117675 RTMG1_8_A07.g1_A029 Roots minus magn... 43 3e-025
gb|DR119366.1|DR119366 RTMG1_22_G02.g1_A029 Roots minus mag... 43 3e-025
gb|CX653171.1|CX653171 COLD1_63_G09.g1_A029 Root cold Pinus... 43 3e-023
>gb|BX679712.1|BX679712 BX679712 RS Pinus pinaster cDNA clone RS32A09, mRNA sequence
Length = 480
Score = 80.3 bits (169), Expect(3) = 3e-037
Identities = 29/58 (50%), Positives = 35/58 (60%)
Frame = +1 / +3
Query: 265 FTIHGLWPDYDDGTWPSCCRRTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLF 438
FTIHGLWPDY+DG+WP CC F+ KI L L++ WPSL S C +G F
Sbjct: 270 FTIHGLWPDYNDGSWPQCCNGPSFDESKIASLHGELERDWPSLSFSSPSDCHGKRGHF 443
Score = 74.4 bits (156), Expect(3) = 3e-037
Identities = 23/30 (76%), Positives = 27/30 (90%)
Frame = +1 / +3
Query: 154 REFDYFALSLQWPGTICASTRHCCATNGCC 243
REFDYF LSL+WPGT+C +T+HCCATN CC
Sbjct: 156 REFDYFVLSLEWPGTVCKNTKHCCATNACC 245
Score = 39.6 bits (80), Expect(3) = 3e-037
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = +1 / +2
Query: 427 KGLFWAHEWEKHGTCS 474
K FW HEWEKHGTC+
Sbjct: 431 KRSFWEHEWEKHGTCA 478
>gb|DT633007.1|DT633007 EST1147938 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFQ19 3' end, mRNA sequence
Length = 839
Score = 45.1 bits (92), Expect(5) = 2e-025
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +1 / +1
Query: 250 EPLQTFTIHGLWPDYDDGTWPSCC 321
+P F+IHGLWP+Y +G WP C
Sbjct: 208 KPASDFSIHGLWPNYSNGKWPQFC 279
Score = 40.9 bits (83), Expect(5) = 2e-025
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +1 / +1
Query: 436 FWAHEWEKHGTCS 474
FW HEWEKHGTCS
Sbjct: 382 FWGHEWEKHGTCS 420
Score = 37.3 bits (75), Expect(5) = 2e-025
Identities = 15/31 (48%), Positives = 18/31 (58%)
Frame = +1 / +1
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKS 408
C +F+ KI LKE L+KYW SL C S
Sbjct: 277 CDSSREFDYSKISDLKEELNKYWDSLSCPSS 369
Score = 34.1 bits (68), Expect(5) = 2e-025
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1 / +1
Query: 154 REFDYFALSLQWPGTICASTRHCC 225
R D++ QWPG+ C + R CC
Sbjct: 121 RSLDFYYFVQQWPGSYCDTRRGCC 192
Score = 30.9 bits (61), Expect(5) = 2e-025
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = +1 / +1
Query: 580 NGKEYALSDVIDTIKHAFGGSPQIVCKRGSIEELRL 687
+G+EY+L ++ + I+ + G P+I C R E +L
Sbjct: 517 DGREYSLRNIKEAIRESTGQLPRIECNRSEEGEHQL 624
>gb|DR685771.1|DR685771 EST1075848 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAB769 3' end, mRNA sequence
Length = 835
Score = 45.1 bits (92), Expect(5) = 2e-025
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +1 / +2
Query: 250 EPLQTFTIHGLWPDYDDGTWPSCC 321
+P F+IHGLWP+Y +G WP C
Sbjct: 200 KPASDFSIHGLWPNYSNGKWPQFC 271
Score = 40.9 bits (83), Expect(5) = 2e-025
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +1 / +2
Query: 436 FWAHEWEKHGTCS 474
FW HEWEKHGTCS
Sbjct: 374 FWGHEWEKHGTCS 412
Score = 37.3 bits (75), Expect(5) = 2e-025
Identities = 15/31 (48%), Positives = 18/31 (58%)
Frame = +1 / +2
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKS 408
C +F+ KI LKE L+KYW SL C S
Sbjct: 269 CDSSREFDYSKISDLKEELNKYWDSLSCPSS 361
Score = 34.1 bits (68), Expect(5) = 2e-025
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1 / +2
Query: 154 REFDYFALSLQWPGTICASTRHCC 225
R D++ QWPG+ C + R CC
Sbjct: 113 RSLDFYYFVQQWPGSYCDTRRGCC 184
Score = 30.9 bits (61), Expect(5) = 2e-025
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = +1 / +2
Query: 580 NGKEYALSDVIDTIKHAFGGSPQIVCKRGSIEELRL 687
+G+EY+L ++ + I+ + G P+I C R E +L
Sbjct: 509 DGREYSLRNIKEAIRESTGQLPRIECNRSEEGEHQL 616
>gb|DR023234.1|DR023234 STRS1_56_G08.b1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_56_G08_A034 3', mRNA sequence
Length = 754
Score = 45.1 bits (92), Expect(5) = 2e-025
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +1 / -2
Query: 250 EPLQTFTIHGLWPDYDDGTWPSCC 321
+P F+IHGLWP+Y +G WP C
Sbjct: 531 KPASDFSIHGLWPNYSNGKWPQFC 460
Score = 40.9 bits (83), Expect(5) = 2e-025
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +1 / -2
Query: 436 FWAHEWEKHGTCS 474
FW HEWEKHGTCS
Sbjct: 357 FWGHEWEKHGTCS 319
Score = 37.3 bits (75), Expect(5) = 2e-025
Identities = 15/31 (48%), Positives = 18/31 (58%)
Frame = +1 / -2
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKS 408
C +F+ KI LKE L+KYW SL C S
Sbjct: 462 CDSSREFDYSKISDLKEELNKYWDSLSCPSS 370
Score = 34.1 bits (68), Expect(5) = 2e-025
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1 / -2
Query: 154 REFDYFALSLQWPGTICASTRHCC 225
R D++ QWPG+ C + R CC
Sbjct: 618 RSLDFYYFVQQWPGSYCDTRRGCC 547
Score = 30.9 bits (61), Expect(5) = 2e-025
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = +1 / -2
Query: 580 NGKEYALSDVIDTIKHAFGGSPQIVCKRGSIEELRL 687
+G+EY+L ++ + I+ + G P+I C R E +L
Sbjct: 222 DGREYSLRNIKEAIRESTGQLPRIECNRSEEGEHQL 115
>gb|DR117606.1|DR117606 RTMG1_8_A07.b1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_8_A07_A029 3', mRNA sequence
Length = 860
Score = 43.2 bits (88), Expect(5) = 3e-025
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1 / +1
Query: 250 EPLQTFTIHGLWPDYDDGTWPSCC 321
+P F+IHGLWP+Y G WP C
Sbjct: 226 KPASDFSIHGLWPNYRTGKWPQFC 297
Score = 39.6 bits (80), Expect(5) = 3e-025
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +1 / +1
Query: 436 FWAHEWEKHGTCS 474
FW HEW+KHGTCS
Sbjct: 400 FWGHEWDKHGTCS 438
Score = 36.8 bits (74), Expect(5) = 3e-025
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1 / +1
Query: 154 REFDYFALSLQWPGTICASTRHCC 225
R FD++ QWPG+ C + R CC
Sbjct: 139 RSFDFYYFVQQWPGSYCDTRRGCC 210
Score = 35.0 bits (70), Expect(5) = 3e-025
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +1 / +1
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKS 408
C +F+ KI L+E L+KYW SL C S
Sbjct: 295 CDSSREFDYSKISDLEEELNKYWGSLSCPSS 387
Score = 33.1 bits (66), Expect(5) = 3e-025
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1 / +1
Query: 580 NGKEYALSDVIDTIKHAFGGSPQIVCKR 663
+G EY+LSD+ + IK + G P I C R
Sbjct: 535 DGSEYSLSDIKEAIKQSTGQLPGIDCNR 618
>gb|DR117675.1|DR117675 RTMG1_8_A07.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_8_A07_A029 5', mRNA sequence
Length = 835
Score = 43.2 bits (88), Expect(5) = 3e-025
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1 / +3
Query: 250 EPLQTFTIHGLWPDYDDGTWPSCC 321
+P F+IHGLWP+Y G WP C
Sbjct: 231 KPASDFSIHGLWPNYRTGKWPQFC 302
Score = 39.6 bits (80), Expect(5) = 3e-025
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +1 / +3
Query: 436 FWAHEWEKHGTCS 474
FW HEW+KHGTCS
Sbjct: 405 FWGHEWDKHGTCS 443
Score = 36.8 bits (74), Expect(5) = 3e-025
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1 / +3
Query: 154 REFDYFALSLQWPGTICASTRHCC 225
R FD++ QWPG+ C + R CC
Sbjct: 144 RSFDFYYFVQQWPGSYCDTRRGCC 215
Score = 35.0 bits (70), Expect(5) = 3e-025
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +1 / +3
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKS 408
C +F+ KI L+E L+KYW SL C S
Sbjct: 300 CDSSREFDYSKISDLEEELNKYWGSLSCPSS 392
Score = 33.1 bits (66), Expect(5) = 3e-025
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1 / +3
Query: 580 NGKEYALSDVIDTIKHAFGGSPQIVCKR 663
+G EY+LSD+ + IK + G P I C R
Sbjct: 540 DGSEYSLSDIKEAIKQSTGQLPGIDCNR 623
>gb|DR119366.1|DR119366 RTMG1_22_G02.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_22_G02_A029 5', mRNA sequence
Length = 798
Score = 43.2 bits (88), Expect(5) = 3e-025
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1 / +2
Query: 250 EPLQTFTIHGLWPDYDDGTWPSCC 321
+P F+IHGLWP+Y G WP C
Sbjct: 242 KPASDFSIHGLWPNYRTGKWPQFC 313
Score = 39.6 bits (80), Expect(5) = 3e-025
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +1 / +2
Query: 436 FWAHEWEKHGTCS 474
FW HEW+KHGTCS
Sbjct: 416 FWGHEWDKHGTCS 454
Score = 36.8 bits (74), Expect(5) = 3e-025
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1 / +2
Query: 154 REFDYFALSLQWPGTICASTRHCC 225
R FD++ QWPG+ C + R CC
Sbjct: 155 RSFDFYYFVQQWPGSYCDTRRGCC 226
Score = 35.0 bits (70), Expect(5) = 3e-025
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +1 / +2
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKS 408
C +F+ KI L+E L+KYW SL C S
Sbjct: 311 CDSSREFDYSKISDLEEELNKYWGSLSCPSS 403
Score = 33.1 bits (66), Expect(5) = 3e-025
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1 / +2
Query: 580 NGKEYALSDVIDTIKHAFGGSPQIVCKR 663
+G EY+LSD+ + IK + G P I C R
Sbjct: 551 DGSEYSLSDIKEAIKQSTGQLPGIDCNR 634
>gb|CX653171.1|CX653171 COLD1_63_G09.g1_A029 Root cold Pinus taeda cDNA clone
COLD1_63_G09_A029 5', mRNA sequence
Length = 807
Score = 43.2 bits (88), Expect(5) = 3e-023
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1 / +1
Query: 250 EPLQTFTIHGLWPDYDDGTWPSCC 321
+P F+IHGLWP+Y G WP C
Sbjct: 250 KPASDFSIHGLWPNYRTGKWPQFC 321
Score = 39.6 bits (80), Expect(5) = 3e-023
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +1 / +3
Query: 436 FWAHEWEKHGTCS 474
FW HEW+KHGTCS
Sbjct: 504 FWGHEWDKHGTCS 542
Score = 36.8 bits (74), Expect(5) = 3e-023
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1 / +1
Query: 154 REFDYFALSLQWPGTICASTRHCC 225
R FD++ QWPG+ C + R CC
Sbjct: 163 RSFDFYYFVQQWPGSYCDTRRGCC 234
Score = 33.1 bits (66), Expect(5) = 3e-023
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1 / +1
Query: 580 NGKEYALSDVIDTIKHAFGGSPQIVCKR 663
+G EY+LSD+ + IK + G P I C R
Sbjct: 640 DGSEYSLSDIKEAIKQSTGQLPGIDCNR 723
Score = 27.6 bits (54), Expect(5) = 3e-023
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1 / +3
Query: 346 KILPLKEVLDKYWPSLYCSKS 408
+I L+E L+KYW SL C S
Sbjct: 429 QISDLEEELNKYWGSLSCPSS 491
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 559,007,105
Number of Sequences: 355925
Number of extensions: 8632800
Number of successful extensions: 39856
Number of sequences better than 1.0e-020: 9
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 17407559000
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)