TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440503.2.1
(542 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR071863.1|DR071863 RTDK1_22_C03.g1_A029 Roots, dark Pin... 73 1e-033
gb|DR071782.1|DR071782 RTDK1_22_C03.b1_A029 Roots, dark Pin... 73 2e-033
gb|DR024294.1|DR024294 STRS1_63_H04.g1_A034 Shoot tip pitch... 76 2e-027
gb|DR684227.1|DR684227 EST1074304 Normalized pine embryo li... 76 2e-024
gb|CF671881.1|CF671881 RTCNT1_59_H08.g1_A029 Root control P... 78 1e-021
>gb|DR071863.1|DR071863 RTDK1_22_C03.g1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_22_C03_A029 5', mRNA sequence
Length = 644
Score = 73.5 bits (154), Expect(3) = 1e-033
Identities = 26/63 (41%), Positives = 43/63 (68%)
Frame = +3 / +3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
G P QLIGF+S+H+ QT ++ F V PC HFS E+G++++ GSH ++VG+ ++ +
Sbjct: 444 GAPQKQLIGFRSVHVPGKQTTNITFVVKPCDHFSTVEENGQRLLAIGSHSLLVGDTQYSV 623
Query: 510 SFM 518
S +
Sbjct: 624 SLV 632
Score = 65.7 bits (137), Expect(3) = 1e-033
Identities = 25/39 (64%), Positives = 30/39 (76%)
Frame = +3 / +3
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFAT 119
R + TGYPGRTYRFY G VF+FG GLSYS YS+ F++
Sbjct: 126 RPNHHTGYPGRTYRFYTGKKVFDFGQGLSYSAYSYEFSS 242
Score = 41.8 bits (85), Expect(3) = 1e-033
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +3 / +3
Query: 204 IGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDGS 329
+ + C +LKF + V+NH MDG+H+VL++ + P G+
Sbjct: 324 VENSPCRKLKFRCSILVRNHDNMDGRHAVLLYSKSPATHKGA 449
>gb|DR071782.1|DR071782 RTDK1_22_C03.b1_A029 Roots, dark Pinus taeda cDNA clone
RTDK1_22_C03_A029 3', mRNA sequence
Length = 769
Score = 73.0 bits (153), Expect(3) = 2e-033
Identities = 26/63 (41%), Positives = 43/63 (68%)
Frame = +3 / +2
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
G P QLIGF+S+H+ QT ++ F V PC HFS E+G++++ GSH ++VG+ ++ +
Sbjct: 308 GAPQKQLIGFRSVHVPGKQTTNITFVVKPCDHFSTVEENGQRLLAIGSHSLIVGDTQYSV 487
Query: 510 SFM 518
S +
Sbjct: 488 SLV 496
Score = 65.2 bits (136), Expect(3) = 2e-033
Identities = 24/34 (70%), Positives = 28/34 (82%)
Frame = +3 / +2
Query: 18 TGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFAT 119
TGYPGRTYRFY G VF+FG GLSYS YS+ F++
Sbjct: 5 TGYPGRTYRFYTGKKVFDFGQGLSYSAYSYEFSS 106
Score = 41.8 bits (85), Expect(3) = 2e-033
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +3 / +2
Query: 204 IGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDGS 329
+ + C +LKF + V+NH MDG+H+VL++ + P G+
Sbjct: 188 VENSPCRKLKFRCSILVRNHDNMDGRHAVLLYSKSPATHKGA 313
>gb|DR024294.1|DR024294 STRS1_63_H04.g1_A034 Shoot tip pitch canker susceptible Pinus taeda
cDNA clone STRS1_63_H04_A034 5', mRNA sequence
Length = 841
Score = 75.8 bits (159), Expect(2) = 2e-027
Identities = 29/63 (46%), Positives = 42/63 (66%)
Frame = +3 / -1
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
G P QLIGF+S+H+ QTA V FEV PC H S E+G +V+ GSH ++VG+ ++ +
Sbjct: 475 GAPQKQLIGFRSVHVPGKQTAKVTFEVKPCDHLSTVKENGERVLTIGSHSLIVGDTQYPV 296
Query: 510 SFM 518
S +
Sbjct: 295 SLV 287
Score = 68.4 bits (143), Expect(2) = 2e-027
Identities = 25/39 (64%), Positives = 32/39 (82%)
Frame = +3 / -1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFAT 119
R + TGYPGRTYRFY G VF+FG+GLSYS+YS+ F++
Sbjct: 793 RPNHDTGYPGRTYRFYTGKKVFDFGHGLSYSRYSYEFSS 677
>gb|DR684227.1|DR684227 EST1074304 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAAQ30 3' end, mRNA sequence
Length = 902
Score = 76.2 bits (160), Expect(3) = 2e-024
Identities = 29/42 (69%), Positives = 31/42 (73%)
Frame = +3 / +3
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPP 128
R +P+ GYPGRTYRFY G TVF FGYGLSYS YS F T P
Sbjct: 438 RPNPSRGYPGRTYRFYTGETVFEFGYGLSYSTYSSTFPTSTP 563
Score = 42.8 bits (87), Expect(3) = 2e-024
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +3 / +1
Query: 336 PASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATED 446
P QLIGFQ +++ + ++ V+F +SPC S TED
Sbjct: 790 PKKQLIGFQRVYVESGTSSEVKFLISPCDDLSTVTED 900
Score = 30.4 bits (60), Expect(3) = 2e-024
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +3 / +3
Query: 219 CDRLKFPAVVRVQNHGPMDGKHSVLV 296
C++ KF V V NHGPM K +V
Sbjct: 678 CEKAKFNLQVLVHNHGPMGWKSRCVV 755
>gb|CF671881.1|CF671881 RTCNT1_59_H08.g1_A029 Root control Pinus taeda cDNA clone
RTCNT1_59_H08_A029 5', mRNA sequence
Length = 638
Score = 77.6 bits (163), Expect(2) = 1e-021
Identities = 35/99 (35%), Positives = 50/99 (50%)
Frame = +3 / -2
Query: 204 IGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDGSGRPASQLIGFQSLHLRAT 383
+G C L F VV V+N G G H L+F + P +G P QLIGF + ++AT
Sbjct: 436 VGDTDCKGLLFDFVVEVKNKGTRTGNHVALLFSKTPFLYHIAGSPKKQLIGFNRVEVKAT 257
Query: 384 QTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDE 500
+ ++F V CK S +GR+ + G H + VG E
Sbjct: 256 EYQRIQFRVDVCKDLSIVDREGRRKLLLGMHTLSVGSCE 140
Score = 45.1 bits (92), Expect(2) = 1e-021
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +3 / -2
Query: 36 TYRFYRGPTVFNFGYGLSYSKYSHRFATKP 125
TYRFY G TV++FGYGLSY+ + P
Sbjct: 637 TYRFYTGETVYDFGYGLSYTSFQSSVVFAP 548
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 192,897,872
Number of Sequences: 355925
Number of extensions: 2526814
Number of successful extensions: 40257
Number of sequences better than 1.0e-020: 5
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 7001290530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)