TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419405.2.3
(1600 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus adde... 97 9e-042
gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip libra... 90 1e-037
gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinu... 97 8e-034
gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus adde... 97 8e-034
gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added... 97 8e-034
gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus adde... 97 4e-033
gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus adde... 97 9e-028
gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compr... 92 3e-024
>gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_19_C01_A029 5', mRNA sequence
Length = 821
Score = 97.3 bits (206), Expect(4) = 9e-042
Identities = 37/81 (45%), Positives = 56/81 (69%)
Frame = -3 / +3
Query: 1106 KYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAV 927
K HFDLSGTAF +A G + L ++G++ + F RVPCE+PG I F+V ++ +FA+
Sbjct: 405 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 584
Query: 926 LVEYEDGDGDVVQVDLMESKT 864
L+EYEDGDGD+ V + ++ +
Sbjct: 585 LIEYEDGDGDLRAVYIKQANS 647
Score = 60.2 bits (125), Expect(4) = 9e-042
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = -3 / +3
Query: 1268 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 231 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 371
Score = 44.6 bits (91), Expect(4) = 9e-042
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = -3 / +3
Query: 746 SGKTLVANNVIPANWRPNTFYRSFV 672
+G+TL ANNVIP NW+P YRS V
Sbjct: 747 TGRTLTANNVIPQNWQPGATYRSLV 821
Score = 25.8 bits (50), Expect(4) = 9e-042
Identities = 9/22 (40%), Positives = 11/22 (50%)
Frame = -3 / +3
Query: 1397 NYTASDAAVYWGPWQRARATWY 1332
++ D A WQ A ATWY
Sbjct: 105 HFEKHDLATQGNDWQPATATWY 170
>gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST17B02, mRNA sequence
Length = 598
Score = 90.4 bits (191), Expect(4) = 1e-037
Identities = 37/86 (43%), Positives = 53/86 (61%)
Frame = -3 / +2
Query: 1121 YYPVSKYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNP 942
Y + HFDLSG AFG +A G L +SG + + + R CE+PG I FHV E S
Sbjct: 122 YCAFGRTHFDLSGAAFGHMATNGKTPALLNSGELPVLYRRTLCEYPGKNITFHVTEGSTA 301
Query: 941 VYFAVLVEYEDGDGDVVQVDLMESKT 864
+F++L+ YEDGDGDV + L ++++
Sbjct: 302 YWFSILIGYEDGDGDVGAMHLKQAES 379
Score = 54.7 bits (113), Expect(4) = 1e-037
Identities = 18/33 (54%), Positives = 26/33 (78%)
Frame = -3 / +2
Query: 1226 LFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
+FK G+GCG+CYK++C + SCS + TV+ITD
Sbjct: 8 VFKGGEGCGACYKVKCMEKGSCSEKPVTVVITD 106
Score = 35.4 bits (71), Expect(4) = 1e-037
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -3 / +2
Query: 839 WTPMRESWGSVWRMDTNHRMQPPFSIRIRNESGK 738
W MR WG+ W + N ++ PFS+R+ + K
Sbjct: 386 WMEMRHVWGANWCLLGNDSLKXPFSVRVTTLTSK 487
Score = 33.6 bits (67), Expect(4) = 1e-037
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3 / +2
Query: 746 SGKTLVANNVIPANWRPNTFYRSFVQY 666
S +TL A NVIP +W PN Y++ + +
Sbjct: 482 SKRTLSARNVIPEHWYPNATYKARLNF 562
>gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_9_H09_A029 5', mRNA sequence
Length = 806
Score = 97.3 bits (206), Expect(3) = 8e-034
Identities = 37/81 (45%), Positives = 56/81 (69%)
Frame = -3 / +2
Query: 1106 KYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAV 927
K HFDLSGTAF +A G + L ++G++ + F RVPCE+PG I F+V ++ +FA+
Sbjct: 434 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 613
Query: 926 LVEYEDGDGDVVQVDLMESKT 864
L+EYEDGDGD+ V + ++ +
Sbjct: 614 LIEYEDGDGDLRAVYIKQANS 676
Score = 60.2 bits (125), Expect(3) = 8e-034
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = -3 / +2
Query: 1268 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 260 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 400
Score = 25.8 bits (50), Expect(3) = 8e-034
Identities = 9/22 (40%), Positives = 11/22 (50%)
Frame = -3 / +2
Query: 1397 NYTASDAAVYWGPWQRARATWY 1332
++ D A WQ A ATWY
Sbjct: 134 HFEKHDLATQGNDWQPATATWY 199
>gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 3', mRNA sequence
Length = 793
Score = 97.3 bits (206), Expect(3) = 8e-034
Identities = 37/81 (45%), Positives = 56/81 (69%)
Frame = -3 / -1
Query: 1106 KYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAV 927
K HFDLSGTAF +A G + L ++G++ + F RVPCE+PG I F+V ++ +FA+
Sbjct: 346 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 167
Query: 926 LVEYEDGDGDVVQVDLMESKT 864
L+EYEDGDGD+ V + ++ +
Sbjct: 166 LIEYEDGDGDLRAVYIKQANS 104
Score = 60.2 bits (125), Expect(3) = 8e-034
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = -3 / -1
Query: 1268 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 520 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 380
Score = 25.8 bits (50), Expect(3) = 8e-034
Identities = 9/22 (40%), Positives = 11/22 (50%)
Frame = -3 / -1
Query: 1397 NYTASDAAVYWGPWQRARATWY 1332
++ D A WQ A ATWY
Sbjct: 646 HFEKHDLATQGNDWQPATATWY 581
>gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_8_F09_A029 5', mRNA sequence
Length = 717
Score = 97.3 bits (206), Expect(3) = 8e-034
Identities = 37/81 (45%), Positives = 56/81 (69%)
Frame = -3 / +3
Query: 1106 KYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAV 927
K HFDLSGTAF +A G + L ++G++ + F RVPCE+PG I F+V ++ +FA+
Sbjct: 405 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 584
Query: 926 LVEYEDGDGDVVQVDLMESKT 864
L+EYEDGDGD+ V + ++ +
Sbjct: 585 LIEYEDGDGDLRAVYIKQANS 647
Score = 60.2 bits (125), Expect(3) = 8e-034
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = -3 / +3
Query: 1268 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 231 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 371
Score = 25.8 bits (50), Expect(3) = 8e-034
Identities = 9/22 (40%), Positives = 11/22 (50%)
Frame = -3 / +3
Query: 1397 NYTASDAAVYWGPWQRARATWY 1332
++ D A WQ A ATWY
Sbjct: 105 HFEKHDLATQGNDWQPATATWY 170
>gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_29_B05_A029 5', mRNA sequence
Length = 619
Score = 97.3 bits (206), Expect(3) = 4e-033
Identities = 38/68 (55%), Positives = 49/68 (72%)
Frame = -3 / +1
Query: 1106 KYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAV 927
K HFDLSGT+F +A PG + ++GI+ I F RVPCE+PG I FHV E ++ +FA+
Sbjct: 415 KIHFDLSGTSFTDMASPGTSSDFLNAGIVSILFRRVPCEYPGNNIAFHVNEGASDHWFAI 594
Query: 926 LVEYEDGD 903
LVEYEDGD
Sbjct: 595 LVEYEDGD 618
Score = 60.6 bits (126), Expect(3) = 4e-033
Identities = 21/45 (46%), Positives = 31/45 (68%)
Frame = -3 / +1
Query: 1262 PFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 1128
PF S + G+ LF+ GKGCG+CY+++C ++ CS TV+ITD
Sbjct: 247 PFQSKVAAGSPALFEGGKGCGACYQVKCTENSVCSQTAVTVVITD 381
Score = 23.1 bits (44), Expect(3) = 4e-033
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -3 / +1
Query: 1358 WQRARATWY 1332
WQ A ATWY
Sbjct: 154 WQPATATWY 180
>gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 5', mRNA sequence
Length = 707
Score = 97.3 bits (206), Expect(2) = 9e-028
Identities = 37/81 (45%), Positives = 56/81 (69%)
Frame = -3 / -2
Query: 1106 KYHFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAV 927
K HFDLSGTAF +A G + L ++G++ + F RVPCE+PG I F+V ++ +FA+
Sbjct: 703 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 524
Query: 926 LVEYEDGDGDVVQVDLMESKT 864
L+EYEDGDGD+ V + ++ +
Sbjct: 523 LIEYEDGDGDLRAVYIKQANS 461
Score = 47.8 bits (98), Expect(2) = 9e-028
Identities = 17/27 (62%), Positives = 20/27 (74%)
Frame = -3 / -2
Query: 746 SGKTLVANNVIPANWRPNTFYRSFVQY 666
+G+TL ANNVIP NW+P YRS V Y
Sbjct: 361 TGRTLTANNVIPQNWQPGATYRSLVNY 281
>gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compression wood Inclined) Pinus taeda
cDNA clone NXCI_022_D05 5' similar to Arabidopsis
thaliana sequence At4g28250 putative
beta-expansin/allergen protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA sequence
Length = 458
Score = 92.3 bits (195), Expect(3) = 3e-024
Identities = 37/77 (48%), Positives = 51/77 (66%)
Frame = -3 / +1
Query: 1100 HFDLSGTAFGRLAKPGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAVLV 921
HFDLSG AFGR+A G L ++G + + + R CE+PG I FHV E S +F++L+
Sbjct: 13 HFDLSGAAFGRMATNGKTPALLNTGELPVLYRRTLCEYPGKNITFHVTEGSTAYWFSILI 192
Query: 920 EYEDGDGDVVQVDLMES 870
EYEDGDGDV + L ++
Sbjct: 193 EYEDGDGDVGAMHLKQA 243
Score = 33.1 bits (66), Expect(3) = 3e-024
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3 / +1
Query: 746 SGKTLVANNVIPANWRPNTFYRSFVQY 666
S +TL A NVIP +W PN Y++ + +
Sbjct: 352 SKRTLSARNVIPDHWYPNATYKARLNF 432
Score = 25.4 bits (49), Expect(3) = 3e-024
Identities = 9/34 (26%), Positives = 15/34 (44%)
Frame = -3 / +1
Query: 839 WTPMRESWGSVWRMDTNHRMQPPFSIRIRNESGK 738
W M WG+ W + ++ PFS + + K
Sbjct: 256 WMEMNHVWGANWCLLGGTPLKAPFSXXVTTLTSK 357
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 670,555,073
Number of Sequences: 355925
Number of extensions: 8815565
Number of successful extensions: 53265
Number of sequences better than 1.0e-020: 10
length of database: 72,425,745
effective HSP length: 54
effective length of database: 53,205,795
effective search space used: 25432370010
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)