TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419405.2.1
(1293 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus adde... 100 7e-046
gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip libra... 92 5e-039
gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinu... 100 9e-039
gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus adde... 100 9e-039
gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added... 100 9e-039
gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus adde... 95 4e-036
gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus adde... 100 7e-028
gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compr... 94 1e-025
>gb|DR387113.1|DR387113 RTHG1_19_C01.g1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_19_C01_A029 5', mRNA sequence
Length = 821
Score = 99.6 bits (211), Expect(4) = 7e-046
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +1 / +3
Query: 448 KYHFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSPVYFAV 627
K HFDLSGTAF +A +G + L ++G++ + F RVPCE+PG I F+V +S +FA+
Sbjct: 405 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 584
Query: 628 LVEYEDGDGDVVQVDLMESKT 690
L+EYEDGDGD+ V + ++ +
Sbjct: 585 LIEYEDGDGDLRAVYIKQANS 647
Score = 60.2 bits (125), Expect(4) = 7e-046
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +1 / +3
Query: 286 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 426
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 231 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 371
Score = 41.8 bits (85), Expect(4) = 7e-046
Identities = 15/25 (60%), Positives = 18/25 (72%)
Frame = +1 / +3
Query: 808 SGKTLVARNVIPANWRPNTFYRSFV 882
+G+TL A NVIP NW+P YRS V
Sbjct: 747 TGRTLTANNVIPQNWQPGATYRSLV 821
Score = 40.0 bits (81), Expect(4) = 7e-046
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1 / +3
Query: 136 AADAGAENFNTSEAAVYWGPWQKARATWYGQPNGAGPD 249
+A A +F + A WQ A ATWYG P+G G D
Sbjct: 84 SAIAYGVHFEKHDLATQGNDWQPATATWYGSPDGDGSD 197
>gb|AW011133.1|AW011133 ST17B02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST17B02, mRNA sequence
Length = 598
Score = 92.3 bits (195), Expect(4) = 5e-039
Identities = 37/86 (43%), Positives = 55/86 (63%)
Frame = +1 / +2
Query: 433 YYPVSKYHFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSP 612
Y + HFDLSG AFG +A +G L +SG + + + R CE+PG I FHV E S+
Sbjct: 122 YCAFGRTHFDLSGAAFGHMATNGKTPALLNSGELPVLYRRTLCEYPGKNITFHVTEGSTA 301
Query: 613 VYFAVLVEYEDGDGDVVQVDLMESKT 690
+F++L+ YEDGDGDV + L ++++
Sbjct: 302 YWFSILIGYEDGDGDVGAMHLKQAES 379
Score = 54.7 bits (113), Expect(4) = 5e-039
Identities = 18/33 (54%), Positives = 26/33 (78%)
Frame = +1 / +2
Query: 328 LFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 426
+FK G+GCG+CYK++C + SCS + TV+ITD
Sbjct: 8 VFKGGEGCGACYKVKCMEKGSCSEKPVTVVITD 106
Score = 35.9 bits (72), Expect(4) = 5e-039
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +1 / +2
Query: 808 SGKTLVARNVIPANWRPNTFYRSFVQY 888
S +TL ARNVIP +W PN Y++ + +
Sbjct: 482 SKRTLSARNVIPEHWYPNATYKARLNF 562
Score = 35.4 bits (71), Expect(4) = 5e-039
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +1 / +2
Query: 715 WAPMRESWGSVWRMDTNHRMQPPFSIRIRNESGK 816
W MR WG+ W + N ++ PFS+R+ + K
Sbjct: 386 WMEMRHVWGANWCLLGNDSLKXPFSVRVTTLTSK 487
>gb|CO158884.1|CO158884 FLD1_9_H09.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_9_H09_A029 5', mRNA sequence
Length = 806
Score = 99.6 bits (211), Expect(3) = 9e-039
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +1 / +2
Query: 448 KYHFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSPVYFAV 627
K HFDLSGTAF +A +G + L ++G++ + F RVPCE+PG I F+V +S +FA+
Sbjct: 434 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 613
Query: 628 LVEYEDGDGDVVQVDLMESKT 690
L+EYEDGDGD+ V + ++ +
Sbjct: 614 LIEYEDGDGDLRAVYIKQANS 676
Score = 60.2 bits (125), Expect(3) = 9e-039
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +1 / +2
Query: 286 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 426
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 260 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 400
Score = 40.0 bits (81), Expect(3) = 9e-039
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1 / +2
Query: 136 AADAGAENFNTSEAAVYWGPWQKARATWYGQPNGAGPD 249
+A A +F + A WQ A ATWYG P+G G D
Sbjct: 113 SAIAYGVHFEKHDLATQGNDWQPATATWYGSPDGDGSD 226
>gb|DR743783.1|DR743783 RTCU1_18_D05.b1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 3', mRNA sequence
Length = 793
Score = 99.6 bits (211), Expect(3) = 9e-039
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +1 / -1
Query: 448 KYHFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSPVYFAV 627
K HFDLSGTAF +A +G + L ++G++ + F RVPCE+PG I F+V +S +FA+
Sbjct: 346 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 167
Query: 628 LVEYEDGDGDVVQVDLMESKT 690
L+EYEDGDGD+ V + ++ +
Sbjct: 166 LIEYEDGDGDLRAVYIKQANS 104
Score = 60.2 bits (125), Expect(3) = 9e-039
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +1 / -1
Query: 286 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 426
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 520 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 380
Score = 40.0 bits (81), Expect(3) = 9e-039
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1 / -1
Query: 136 AADAGAENFNTSEAAVYWGPWQKARATWYGQPNGAGPD 249
+A A +F + A WQ A ATWYG P+G G D
Sbjct: 667 SAIAYGVHFEKHDLATQGNDWQPATATWYGSPDGDGSD 554
>gb|DR385457.1|DR385457 RTHG1_8_F09.g1_A029 Roots plus added mercury Pinus taeda cDNA clone
RTHG1_8_F09_A029 5', mRNA sequence
Length = 717
Score = 99.6 bits (211), Expect(3) = 9e-039
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +1 / +3
Query: 448 KYHFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSPVYFAV 627
K HFDLSGTAF +A +G + L ++G++ + F RVPCE+PG I F+V +S +FA+
Sbjct: 405 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 584
Query: 628 LVEYEDGDGDVVQVDLMESKT 690
L+EYEDGDGD+ V + ++ +
Sbjct: 585 LIEYEDGDGDLRAVYIKQANS 647
Score = 60.2 bits (125), Expect(3) = 9e-039
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +1 / +3
Query: 286 QYPFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 426
Q PF S + G+ LFK G GCG+CY+++C ++ CS TV+ITD
Sbjct: 231 QSPFQSKIAAGSPALFKGGTGCGACYQVKCTENGLCSQTAVTVVITD 371
Score = 40.0 bits (81), Expect(3) = 9e-039
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1 / +3
Query: 136 AADAGAENFNTSEAAVYWGPWQKARATWYGQPNGAGPD 249
+A A +F + A WQ A ATWYG P+G G D
Sbjct: 84 SAIAYGVHFEKHDLATQGNDWQPATATWYGSPDGDGSD 197
>gb|DR745426.1|DR745426 RTCU1_29_B05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_29_B05_A029 5', mRNA sequence
Length = 619
Score = 95.0 bits (201), Expect(3) = 4e-036
Identities = 38/68 (55%), Positives = 48/68 (70%)
Frame = +1 / +1
Query: 448 KYHFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSPVYFAV 627
K HFDLSGT+F +A G + ++GI+ I F RVPCE+PG I FHV E +S +FA+
Sbjct: 415 KIHFDLSGTSFTDMASPGTSSDFLNAGIVSILFRRVPCEYPGNNIAFHVNEGASDHWFAI 594
Query: 628 LVEYEDGD 651
LVEYEDGD
Sbjct: 595 LVEYEDGD 618
Score = 60.6 bits (126), Expect(3) = 4e-036
Identities = 21/45 (46%), Positives = 31/45 (68%)
Frame = +1 / +1
Query: 292 PFMSMGSCGNQPLFKDGKGCGSCYKIRCRKDPSCSGRTETVIITD 426
PF S + G+ LF+ GKGCG+CY+++C ++ CS TV+ITD
Sbjct: 247 PFQSKVAAGSPALFEGGKGCGACYQVKCTENSVCSQTAVTVVITD 381
Score = 35.0 bits (70), Expect(3) = 4e-036
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1 / +1
Query: 196 WQKARATWYGQPNGAGPD 249
WQ A ATWYG P+G G +
Sbjct: 154 WQPATATWYGSPDGDGSE 207
>gb|DR743865.1|DR743865 RTCU1_18_D05.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_18_D05_A029 5', mRNA sequence
Length = 707
Score = 99.6 bits (211), Expect(2) = 7e-028
Identities = 38/81 (46%), Positives = 57/81 (70%)
Frame = +1 / -2
Query: 448 KYHFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSPVYFAV 627
K HFDLSGTAF +A +G + L ++G++ + F RVPCE+PG I F+V +S +FA+
Sbjct: 703 KKHFDLSGTAFTDMASAGASSNLLNTGVVSVLFRRVPCEYPGKNIAFNVNGGASDYWFAI 524
Query: 628 LVEYEDGDGDVVQVDLMESKT 690
L+EYEDGDGD+ V + ++ +
Sbjct: 523 LIEYEDGDGDLRAVYIKQANS 461
Score = 45.5 bits (93), Expect(2) = 7e-028
Identities = 18/32 (56%), Positives = 21/32 (65%)
Frame = +1 / -2
Query: 808 SGKTLVARNVIPANWRPNTFYRSFVQYS*LAS 903
+G+TL A NVIP NW+P YRS V Y * S
Sbjct: 361 TGRTLTANNVIPQNWQPGATYRSLVNYH*SPS 266
>gb|BE496299.1|BE496299 NXCI_022_D05_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_022_D05 5' similar to Arabidopsis
thaliana sequence At4g28250 putative
beta-expansin/allergen protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 458
Score = 94.1 bits (199), Expect(3) = 1e-025
Identities = 37/77 (48%), Positives = 53/77 (68%)
Frame = +1 / +1
Query: 454 HFDLSGTAFGRLAKSGLNDKLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSSPVYFAVLV 633
HFDLSG AFGR+A +G L ++G + + + R CE+PG I FHV E S+ +F++L+
Sbjct: 13 HFDLSGAAFGRMATNGKTPALLNTGELPVLYRRTLCEYPGKNITFHVTEGSTAYWFSILI 192
Query: 634 EYEDGDGDVVQVDLMES 684
EYEDGDGDV + L ++
Sbjct: 193 EYEDGDGDVGAMHLKQA 243
Score = 35.4 bits (71), Expect(3) = 1e-025
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +1 / +1
Query: 808 SGKTLVARNVIPANWRPNTFYRSFVQY 888
S +TL ARNVIP +W PN Y++ + +
Sbjct: 352 SKRTLSARNVIPDHWYPNATYKARLNF 432
Score = 25.4 bits (49), Expect(3) = 1e-025
Identities = 9/34 (26%), Positives = 15/34 (44%)
Frame = +1 / +1
Query: 715 WAPMRESWGSVWRMDTNHRMQPPFSIRIRNESGK 816
W M WG+ W + ++ PFS + + K
Sbjct: 256 WMEMNHVWGANWCLLGGTPLKAPFSXXVTTLTSK 357
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 503,746,057
Number of Sequences: 355925
Number of extensions: 6799972
Number of successful extensions: 43818
Number of sequences better than 1.0e-020: 10
length of database: 72,425,745
effective HSP length: 54
effective length of database: 53,205,795
effective search space used: 20005378920
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)